The Dinaridic segment of Neotethys was affected by a widespread shortening and related subduction-accretion-obduction processes that commenced in the middle Jurassic. In the Dinarides, the Krivaja-Konjuh Ophiolite Complex (KKOC) stands as the largest ophiolite complex with a well-exposed metamorphic sole which is the key to understanding the dynamics of intraoceanic subduction initiation in this part of Neotethys. In this contribution we present Sm-Nd geochronology on a granulite facies amphibolite from the KKOC, as well as a detailed petrological description. A five-point isochrone age calculated from clinopyroxene, plagioclase, garnet, amphibole and whole rock is 162 ± 14 Ma (MSWD = 6.2), whereas garnet and whole rock yield 160 ± 7 Ma. Ages calculated from all data points except clinopyroxene are 162 ± 5 Ma (MSWD = 1.09). Petrographic investigations suggest that these ages date granulite facies metamorphic conditions (i.e. peak metamorphism of Grt-Cpx amphibolite) rather than post-peak exhumation or obduction processes. Phase textural relationships are in line with previous research, which indicated a peak metamorphism equilibration pressure and temperature of ~1 GPa and ~800 °C, respectively. Granulite facies conditions are elucidated for an igneous precursor, which underwent a multi-stage metamorphism that gave rise to recrystallization of igneous clinopyroxene and plagioclase, epitaxial growth of amphibole, and garnet blastosis. Taking into account the age of gabbronorite from the youngest segment of the KKOC oceanic crust (Taorcian to Bathonian) and ages of radiolarian assemblages from the KKOC melange (Bajocian to Bathonian), it may be inferred that within ~25 Ma the Dinaridic segment of Neotethys evolved rapidly from active ridge spreading through a stage of intraoceanic subduction and arc magmatism toward sub-ophioliteexhumation and further obduction along the Adria passive margin at the end of the Jurassic era.

This paper presents the methodology for the development of an optimization model for the optimization of the cross-section dimensions of a bridge crane girder designed as a welded I-profile. To carry out this optimization, the CAD/CAE software package CATIA V5 was used. In order to develop an optimization model, a CAD geometrical model and structural analysis model were developed. Optimization was carried out by the iterative method using a simulated hardening algorithm. Additionally, the optimization process is carried out by using the PEO (Product Engineering Optimization) CATIA module that contains tools for setting the optimization criteria, design parameters, constraints, and algorithms. The goal of the optimization is to achieve the minimal mass of the girder, while satisfying all functional and geometrical constraints. As a result of the optimization process, minimal girder dimensions were obtained and due to that, a minimal amount of material can be used for the manufacturing of the girder.

Abstract Background We have developed a new platform named "Biologer" intended for recording species observations in the field (but also from literature resources and collections). The platform is created as user-friendly, open source, multilingual software that is compatible with Darwin Core standard and accompanied by a simple Android application. It is made from the user’s perspective, allowing everyone to choose how they share the data. Project team members are delegated by involved organisations. The team is responsible for development of the platform, while local Biologer communities are engaged in data collection and verification. New information Biologer has been online and available for use in Serbia since 2018 and was soon adopted in Croatia and Bosnia and Herzegovina. In total, we have assembled 536 users, who have collected 163,843 species observation records data from the field and digitalised 33,458 literature records. The number of active users and their records is growing daily. Out of the total number of gathered data, 89% has been made open access by the users, 10% is accessible on the scale of 10×10 km and only 1% is closed. In the future, we plan to provide a taxonomic data portal that could be used by local and national initiatives in Eastern Europe, aggregate all data into a single web location, create detailed data overview and enable fluent communication between users.

The biological performance of flocculent sludge in sequencing batch reactor for the treatment of fish canning wastewater was evaluated in terms of organic matter and nutrient removal by gradual increase of salt concentration in the nitritation-denitritation process. Salinity negatively affected the biological system performance in a way that reduced organic and nutrient removal. The removal efficiency of organic matter and nitrogen showed good performance below 20 g NaCl/L, while phosphate accumulating organisms activity was deteriorated and declined during whole experiment. Nitrogen removal occurred as ammonium oxidation with nitrite accumulation. Nitrite reduction was not affected by salt concentration.

Sequencing for seismic structures Structures illuminated by seismic waves at the core-mantle boundary of the Earth are traditionally found by focusing on a specific target area. Kim et al. used an unsupervised manifold learning algorithm called “the Sequencer” to automatically detect anomalies in seismic data (see the Perspective by Miller). Using this technique, they uncovered structures at the core-mantle boundary across the entire Pacific region all at once. They found many structures previously identified, but also a new, ultra-low-velocity zone beneath the Marquesas Islands. Science, this issue p. 1223; see also p. 1183 An unsupervised machine learning algorithm uncovers the structure of the core-mantle boundary region under the Pacific. Scattering of seismic waves can reveal subsurface structures but usually in a piecemeal way focused on specific target areas. We used a manifold learning algorithm called “the Sequencer” to simultaneously analyze thousands of seismograms of waves diffracting along the core-mantle boundary and obtain a panoptic view of scattering across the Pacific region. In nearly half of the diffracting waveforms, we detected seismic waves scattered by three-dimensional structures near the core-mantle boundary. The prevalence of these scattered arrivals shows that the region hosts pervasive lateral heterogeneity. Our analysis revealed loud signals due to a plume root beneath Hawaii and a previously unrecognized ultralow-velocity zone beneath the Marquesas Islands. These observations illustrate how approaches flexible enough to detect robust patterns with little to no user supervision can reveal distinctive insights into the deep Earth.

In this paper we present two different, software and reconfigurable hardware, open architecture approaches to the PUMA 560 robot controller implementation, fully document them and provide the full design specification, software code and hardware description. Such solutions are necessary in today’s robotics and industry: deprecated old control units render robotic installations useless and allow no upgrades, advancements, or innovation in an inherently innovative ecosystem. For the sake of simplicity, just the first robot axis is considered. The first approach described is a PC solution with data acquisition I/O board (Humusoft MF634). This board is supported with Matlab Real-Time Windows Toolbox for real-time applications and thus whole controller was designed in Matlab environment. The second approach is a robot controller developed on field programmable gate arrays (FPGA) board. The complexity of FPGA design can be overcome by using a third party software package, such as self-developed Matlab FPGA Real Time Toolbox. In both cases, parameters of motion controller are calculated by using simulation of the PUMA 560 robot first axis motion. Simulations were conducted in Matlab/Simulink using Robotics Toolbox.

The aim of this research was to determine the influence of motor abilities on the success of performing a freestyle swimming, pre and post the completed swimming training program. The study was conducted on a sample of 90 female students, aged 11-12 years, fifth graders from primary schools in Novi Grad Municipality in Sarajevo, using 6 motor variables and 1 criterion. Regression analysis was used to determine the effect of balance and flexibility on the freestyle swimming performance. Analyzing the presented results of the regression analysis, it was found that after the pre-test measurement of the variables for estimating the balance, the following predictor motor variable had the greatest and statistically significant influence on the criterion variable: standing upright on the balance beam with eyes open (BETA) = .581, which is significant at the level of p<.001. For flexibility, the following predictor motor variable is: upper body bent forward while sitting with legs side by side (BETA) = .411, which is significant at the p<.001 level. After the post-test measurement of the variables for the assessment of motor abilities (balance and flexibility), the same variables as for the pre-test measurement had the statistically significant influence on the criterion variable (freestyle swimming of the sections 25 m long – crawl, chest or back swimming). At standing on the right leg longitudinally, open-eyed, on the balance bench (BETA) = .448, which is significant at the level of p<.001, and in flexibility the variable - bench ab with legs side by side (BETA) = .463, which is significant at the level of p<.001. All movements in swimming require well-developed flexibility (mobility), and in particular the mobility of the torso and upper extremities.

Background: Variants in recombination-activating genes (RAG) are common genetic causes of autosomal recessive forms of combined immunodeficiencies (CID) ranging from severe combined immunodeficiency (SCID), Omenn syndrome (OS), leaky SCID, and CID with granulomas and/or autoimmunity (CID-G/AI), and even milder presentation with antibody deficiency. Objective: We aim to estimate the incidence, clinical presentation, genetic variability, and treatment outcome with geographic distribution of patients with the RAG defects in populations inhabiting South, West, and East Slavic countries. Methods: Demographic, clinical, and laboratory data were collected from RAG-deficient patients of Slavic origin via chart review, retrospectively. Recombinase activity was determined in vitro by flow cytometry-based assay. Results: Based on the clinical and immunologic phenotype, our cohort of 82 patients from 68 families represented a wide spectrum of RAG deficiencies, including SCID (n = 20), OS (n = 37), and LS/CID (n = 25) phenotypes. Sixty-seven (81.7%) patients carried RAG1 and 15 patients (18.3%) carried RAG2 biallelic variants. We estimate that the minimal annual incidence of RAG deficiency in Slavic countries varies between 1 in 180,000 and 1 in 300,000 live births, and it may vary secondary to health care disparities in these regions. In our cohort, 70% (n = 47) of patients with RAG1 variants carried p.K86Vfs*33 (c.256_257delAA) allele, either in homozygous (n = 18, 27%) or in compound heterozygous (n = 29, 43%) form. The majority (77%) of patients with homozygous RAG1 p.K86Vfs*33 variant originated from Vistula watershed area in Central and Eastern Poland, and compound heterozygote cases were distributed among all Slavic countries except Bulgaria. Clinical and immunological presentation of homozygous RAG1 p.K86Vfs*33 cases was highly diverse (SCID, OS, and AS/CID) suggestive of strong influence of additional genetic and/or epigenetic factors in shaping the final phenotype. Conclusion: We propose that RAG1 p.K86Vfs*33 is a founder variant originating from the Vistula watershed region in Poland, which may explain a high proportion of homozygous cases from Central and Eastern Poland and the presence of the variant in all Slavs. Our studies in this cohort of RAG1 founder variants confirm that clinical and immunological phenotypes only partially depend on the underlying genetic defect. As access to HSCT is improving among RAG-deficient patients in Eastern Europe, we anticipate improvements in survival.

COVID-19 pandemic: implications on the surgical treatment of gastrointestinal and hepatopancreatobiliary tumours in Europe Editor In the context of the ongoing COVID19 pandemic, the field of surgical oncology demands careful crisis management considering the overall importance of timely treatment1,2. Based on an online survey (Google Forms; Google LLC, Mountain View, California, USA) conducted among university-affiliated surgical departments throughout Europe, we offer a multicentre perspective on the instantaneous performance of gastrointestinal (GI) and hepatopancreatobiliary (HPB) tumour surgery. Ninety-five surgeons from 79 surgical departments in 20 European countries completed the survey. The survey revealed an alarming status quo. Some 59 per cent of participants stated that the COVID-19 pandemic is having a strong or very strong impact on ongoing treatment of their patients (Fig. 1a). A decrease in patient referral since onset of the viral outbreak was observed by 90 per cent of respondents. Twentynine per cent reported a decrease of more than 60 per cent compared to the pre-pandemic standard (Fig. 1b). In addition to a re-evaluation of hospital capacities, the excessive workload of general practitioners, the role of the media and general recommendations to avoid person-to-person contact, including medical appointments, have to be reconsidered3. It can be assumed that telemedical options are not offered sufficiently (only 12 per cent of respondents reported telemedical options for all outpatient appointments)4. Moreover, almost one-third reported that Fig 1 Impact of the COVID-19 pandemic

ABSTRACT The digital printing process contributes to the quality of indoor air through the emission of chemical stressors such as total volatile organic compounds (TVOCs) and suspended particulate matter (SPM). The concentration levels of SPM and TVOCs were determined using the gravimetric and gas chromatography method, respectively. The results show that the digital printing machines and the cutting machine do not produce PM2.5 particles. The significant cumulative mass concentrations of PM10 suspended particles of 9.91 μg/m3 for a single-colour digital printing machine, 4.45 μg/m3 for a four-colour digital printing machine, and 2.02 μg/m3 for a cutting machine, were detected. By comparing the cumulative values of TVOC concentration levels measured, it can be concluded that the values have been 4 to 12 times higher for the four-colour digital printing machine than for the single-colour digital printing machine. Namely, the TVOC concentration levels measured for the four-colour digital printing machine ranged from 28.69 to 57.30 mg/m3, while the values for the single-colour digital printing machine ranged from 2.31 to 13.51 mg/m3. Considering the fact that there are no standards of quality of indoor air in printing offices defined in the Republic of Serbia, the results obtained could represent a valid basis and a worthy guideline for the definition of values necessary for the indoor air quality assessment.