In this paper, machinability influences from the start to end of final product production in a steel plant were analyzed, including chemical composition, deoxidizing agents and casting parameters, which drastically influence the macrostructure and segregation (i.e., chemical nonhomogeneity) of continuously cast and subsequently rolled material. The data (seven parameters from secondary metallurgy, four parameters from the casting process and the content of ten chemical elements) from the serial production of calcium-treated steel grades (254 batches of 25 different steel grades from January 2018 to March 2020) were used for predicting machinability. Machinability was determined based on ISO 3685:1993, where the machinability of each individual batch is represented as the cutting speed and the tool is worn out within fifteen minutes. For the prediction of these cutting speeds, linear regression and genetic programming were used. Out of 25 analyzed steel grades, 20MnV6 steel grade was the most problematic and also the most often produced. Out of 57 produced batches of 20MnVS6 steel, 23 batches had nonconforming machinability. Based on the modeling results, the steelmaking process was optimized. Consequently, 40 additional batches of 20MnV6 (from March 2020 to July 2020) were subsequently produced based on an optimized steelmaking process. In all 40 cases, the required machinability was achieved without changing other properties required by the customers.

U radu je razvijen matematički model trofaznog, dvonamotajnog energetskog transformatora sprege Dyn5, podesan za proračune ustaljenih složeno­periodičnih režima. Model obezbeđuje uvažavanje postojanja nenultog sprežnog broja transformatora, omogućavajući proračune ustaljenih složenoperiodičnih radnih režima uz istovremeno prisustvo nesimetrije i/ili neuravnoteženosti. Na ilustrativnim primerima je pokazano kakav je uticaj energetskih transformatora na uspostavljanje složenoperiodičnih režima usled harmo­nijskog izobličenja napona napajanja na njegovim primarnim priključcima.

The COVID-19 pandemic has shaken the world unprecedentedly, where it has affected the vast global population both socially and economically. The pandemic has also opened our eyes to the many threats that novel virus infections can pose for humanity. While numerous unknowns are being investigated in terms of the distributed damage that the virus can do to the human body, recent studies have also shown that the infection can lead to lifelong sequelae that could affect other parts of the body, and one example is the brain. As part of this work, we investigate how viral infection can affect the brain by modelling and simulating a neuron's behaviour under demyelination that is affected by the cytokine storm. We quantify the effects of cytokine-induced demyelination on the propagation of action potential signals within a neuron. We used information and communication theory analysis on the signal propagated through the axonal pathway under different intensity levels of demyelination to analyse these effects. Our simulations demonstrate that virus-induced degeneration can play a role in the signal power and spiking rate and the probability of releasing neurotransmitters and compromising the propagation and processing of information between the neurons. We also propose a transfer function that models these attenuation effects that degenerates the action potential, where this model has the potential to be used as a framework for the analysis of virus-induced neurodegeneration that can pave the way to improved understanding of virus-induced demyelination.

The process of travertine formation and carbonate deposition in the rivers is unique, delicate, and depends on the activity of algae and mosses. Although diatoms have been used extensively in hydrobiological studies, the comparative analysis data on diatom communities of the travertine barriers in karstic rivers are still scarce. ‡ § | ¶ ‡ ‡ # § ¤,« »,˄ ˄,» »,˄ © Kamberović J et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The study aimed to detect the diatom composition on travertine barriers in the Una River, the large karstic river in Bosnia and Herzegovina. An integrated classical morphological identification approach with metabarcoding was applied on eight samples across the river length profile. Morphological analyses were performed using both light and scanning electron microscopes. Subsequent DNA metabarcoding of the chloroplastic gene 312bp rbcL was done. The DADA2 pipeline was used for the bioinformatic treatment of the demultiplexed MiSeq reads to infer Amplicon Sequence Variants (ASVs). ASVs were taxonomically assigned using the Diat.barcode v7 reference database. A total of 126 species were identified using the morphological approach, while 133 ASVs were taxonomically assigned to 58 unique taxa with the molecular approach. Diatom community structures in terms of molecular and morphological approaches were congruent with 49 shared species. Species from genera Gomphonema, Navicula and Encyonema were less assigned in molecular analysis. The most abundant taxa in the Una River are alkaliphilous, belonging to the genera Gomphonema, Nitzshia and Navicula. Although specific for their extremely good chemical status, the travertine barriers of the Una River are largely inhabited with meso-eutraphentic taxa.

Chaetopteryx villosa (Fabricius, 1798) is a caddisfly species distributed throughout Europe, except in the Balkan and Apennine Peninsula. However, phylogenetically close species belonging to the C. villosa group are widespread throughout entire Europe. Species of this group (C. villosa, C. gessneri, C. fusca, C. sahlbergi, C. atlantica, C. bosniaca, C. vulture, and C. trinacriae) have distinct distributions with some overlaps. Adult forms of these species are morphologically similar, whereas larval morphology is only known for some species. There are also indications of species hybridization (e.g., C. villosa x fusca). Presumably, the molecular approach for the species determination of this group would be highly beneficial. In the BOLD database, there are 154 specimens with COI-5P barcodes of C. villosa species. Out of the remaining species, C. sahlbergi has 27 specimens with a barcode, C. fusca 20, C. gessneri 5, C. bosniaca 5, and C. atlantica 1, whereas sequences from the species C. vulture and C. trinacriae are missing. Therefore, we tested the power of discrimination of the COI-5P marker in the C. villosa group, as the most common barcoding markers for species identification in animals. Only sequences from public records originating from experienced research groups or taxonomists and containing a specimen photograph were taken as input. A total of 75 ‡,§ ‡,§ ‡,§ ‡,§ ‡,§ © Destanović D et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. sequences from the BOLD database were obtained. Out of these sequences, 11 belonged to C. fusca, 5 to C. gessneri, 52 to C. villosa, 5 to C. bosniaca, and 2 to C. sahlbergi. For the generation of overview trees, COI-5P barcodes of Rhyacophila fasciata and Rh. nubila were used as outgroups. All sequences were trimmed at 5’ and 3’ ends, resulting in a final alignment length of 516 base pairs. Multiple sequence alignments and editing were done in the MEGA-X software. Analysis of nucleotide polymorphism was done in DNASP6 software. MEGA-X was used to calculate the pairwise distance and overall mean pdistance, and to construct the overview trees. Analysis of DNA polymorphism revealed 14 haplotypes of C. villosa, 3 haplotypes of C. fusca, 2 haplotypes of C. gessneri, and one for species C. bosniaca and C. sahlbergi. There were no significant interspecific and intraspecific differences among haplotypes based on pairwise distances. The p-distance between one of the haplotypes of C. fusca and C. villosa was 0.000, whereas the p-distance among haplotypes of C. villosa varied from 0.001 to about 0.055. The mean overall p-distance among haplotypes of all species equaled 0.03. No species-specific clusters were observed when phylogenetic trees were constructed except for C. gessneri, regardless of the method used (i.e., NJ, UPGMA, ML, ME, or MP). To minimize the possibility of species misidentification, we used only records submitted by NTNU-Norwegian University of Science and Technology (Norway), SNSB-Zoologische Staatssammlung Muenchen (Germany), Zoologisches Forschungsmuseum Alexander Koenig (Germany), University of Oulu, Zoological Museum (Finland), prof Hans Malicky and prof Mladen Kučinić. No records identified as hybrids were included in the analyses. With the exception of C. gessneri, COI-5P marker failed to separate the species of the C. villosa group. However, it is highly unlikely that poor species determination was the basis for such a result. To enable the comprehensive and unbiased evaluation of the relationships within this group, data coverage in BOLD database for most of the studied species should be enhanced, encompassing different geographical distribution of samples. Further studies are needed to detect the array of molecular markers suitable for the species delineation in a complex group such as C. villosa.

Bosnia and Herzegovina has valuable natural resources with a high percentage of endemic and autochthonous species (Kučinić et al. 2008, Đug and Drešković 2012). The freshwater fauna of Trichoptera in this area is under-investigated, with a lack of morphological description of different life stages and DNA barcode data. Public data show 58,993 barcode entries for Trichoptera in the Barcode of Life Data Systems (BOLD) submitted from 92 countries, and none from Bosnia and Herzegovina (B&H) (BOLD 2021). Previous research in Bosnia and Herzegovina has provided the first DNA barcode for the endemic species Rhyacophila bosnica, stored in GeneBank, under accession number MK211322 by a domestic institution (Kalamujić Stroil et al. 2018). A few DNA barcodes of adult individuals of Trichoptera from Bosnia and Herzegovina were found in BOLD. However, these specimens were collected on B&H territory, but analyzed, processed, and stored by foreign institutions. To change the current state of DNA barcoding of Trichoptera in Bosnia and Herzegovina, we aimed to employ this approach in investigating caddisflies in selected habitats in the Sarajevo Canton. Our fieldwork was done in all five protected areas (spring of the Bosna River, Bijambare, Trebević, Skakavac, and Bentbaša) in which larvae samples were collected according to ‡,§ ‡,§ ‡,§ ‡,§ ‡ © Destanović D et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. the AQEM sampling methodology. The standard animal DNA barcode was successfully obtained using degenerated primers LCO1490JJ and HCO2198-JJ (Astrin and Stüben 2008). Out of 684 collected individuals (313 Trebević, 130 spring of the Bosna River, 117 Bijambare, 71 Bentbaša, 53 Skakavac), a subset of specimens were sequenced. We uncovered 14 different taxa, 11 genera and six families (Limnephilidae, Glossosomatidae, Rhyacophilidae, Goeridae, Hydropsychidae, Polycentropodidae). The preliminary data of Trichoptera composition in the Sarajevo Canton indicated species richness. Based on our sequential data, a new subspecies was discovered in two investigated areas (Valladolid et al. 2020), proving that Trichoptera species diversity in our country is far from entirely uncovered. The benefit and power of the DNA barcoding approach are that it can pinpoint the areas of vast and unknown species diversity more economically, both financially and temporarily, than the morphological approach. Therefore, we believe that it is critical to support the development of DNA barcoding for the bioassessment of freshwater ecosystems in Bosnia and Herzegovina. Several problems prevented us from exploiting sequential data to the fullest. Despite a general notion among scientists that European Trichoptera species are well covered in the BOLD database, most of the sequences we obtained were absent from the database. Secondly, we recognized that morphological data about the larval developmental stage of B&H Trichoptera species are largely missing. The unified, updated, and complete data on this order of insects is urgently needed. However, insufficient financial support by governmental institutions and lack of systematic approach to barcoding the wildlife of Bosnia and Herzegovina hampers this process. Further attempts to collaborate with the stakeholders can be crucial with profound and substantial implications for biomonitoring of aquatic macroinvertebrates in general. New approaches, such as novel DNA barcoding-based methodology can fill an important gap in our knowledge of Balkan caddisflies haplotypes, lineages, and their diversification and distribution patterns.

Race walking (RW) is a cyclic athletic discipline, of aerobic character, which is characterized by high intensity and high energy consumption. The result is correlated with the anthropological dimensions of the walker as well as good technical performance. The study includes 25 male students, third year of study, Faculty of Physical Education and Sports in East Sarajevo. For the purposes of the research, the results of RW at 2km (criterion variable) were measured, on the basis of which average values of walking speed (WS) were calculated. The aim of the research was to determine the correlation between the achieved result depending on the walking speed. By applying the Pearson product of the correlation moment, the obtained results confirmed a significant and high correlation between the result and the walking speed (r = -0.988; p<0.05) with an inverse function that confirms the high dependence of the result on the WS. Article visualizations:

Current technology for blood glucose level monitoring is mainly based on the invasive finger‐prick extraction of a small drop of blood using a lancet and measured via a handheld glucometer, which is not conducive to continuous measurements. Interstitial fluid (ISF) is gaining attention as an alternative biofluid. Its biochemical composition is very similar to that of blood and it can be monitored in a continuous manner via minimally invasive methods that cause no pain and minimize any risk of infection. Herein, a microneedle array (MNA) based transdermal sensing system for the pain free monitoring of ISF glucose is presented. High‐density silicon microneedles (≈9500 microneedles cm−2) are used to prepare a three‐electrode patch for the electrochemical monitoring of glucose. The MNA glucose patch shows very good selectivity when tested in artificial ISF, with a sensitivity of 0.1622 µA mm−1 cm−2 and a detection limit of 0.66 mm. In vivo application of the microneedle array in mice shows that the ISF glucose concentrations obtained with the MNA sensor gave very good correlation with the blood glucose levels determined with a commercial glucometer. This microneedle‐based sensing system hence provides an alternative transdermal diagnostic tool to the invasive existing techniques.

<p>In order to mitigate climate change and reduce the anthropogenic greenhouse gas (GHG) emissions, the Kyoto protocol has been adopted in 1997 and the Paris Agreement entered into force in 2016. The Paris Agreement have ratified 190 out of 197 Parties of the United Nations Framework Convention on Climate Change (UNFCCC) and Croatia is one of them as well. Each Party has obliged regularly to submit the national inventory report (NIR) providing the information on the national anthropogenic GHG emissions by sources and removals by sinks to the UNFCCC. Reporting under the NIR is divided into six categories / sectors, and one of them is land use, land use change and forestry (LULUCF) sector, where an issue of uncertainty estimates on carbon emissions and removals occurs. As soil respiration represents the second-largest terrestrial carbon flux, the national studies on soil respiration can reduce the uncertainty and improve the estimation of country-level carbon fluxes. Due to the omission of national data, the members of the University of Zagreb Faculty of Agriculture, Department of General Agronomy have started to study soil respiration rates in 2012, and since then many different studies on soil respiration under different agricultural land uses (i.e. annual crops, energy crop and vineyard), management practices (i.e. tillage and fertilization) and climate conditions (i.e. continental and mediterranean) in Croatia have been conducted. The obtained site specific results on field measurements of soil carbon dioxide concentrations by <em>in situ</em> closed static chamber method will be presented in this paper.</p>

<p>Human activities have changed ecosystems and today &#8776; 60% of the world&#8217;s ecosystems are already degraded. These changes have caused growing environmental costs, including biodiversity loss and land degradation, which in turn has resulted in many economic, social and cultural losses. Protected areas (PAs) are the key tool in biodiversity conservation, moreover they may help to maintain water supplies and food security, strengthen climate resilience and improve human health and well-being. International Union for Conservation of Nature (IUCN) defined PA as &#8222;a clearly defined geographical space, recognized, dedicated, and managed, through legal or other effective means, to achieve the long-term conservation of nature with associated ecosystem services (ES) and cultural values&#8221;. Such areas represent Earth systems in which influence of human interactions with preserved ecosystems are readily evident. The coverage of PA is a widely used indicator of sustainable development, because the loss of biodiversity is recognized as one of the most serious global environmental threats. The &#8220;Big Five&#8221; threats to global biodiversity are fragmentation, habitat loss, overexploitation of natural resources, pollution, and the spread of invasive alien species. New interventions for governing nature are captured by the umbrella of nature-based solutions (NBS) in the European Union (EU) policy context. NBS can offer accessible, sustainable, and feasible benefits via a range of areas affecting public health and social well-being. According to IUCN NBS are defined as &#8220;actions to protect, sustainably manage, and restore natural or modified ecosystems, that address societal challenges effectively and adaptively, simultaneously providing human well-being and biodiversity benefits&#8221;. NBS address these societal challenges over the delivery of ES. The main objective of this study is to use the effect of NBS to enhance the sustainability of management of the PAs that would have environmental, social and economic benefits. The methodology includes determination of heavy metals in soils and needles of Picea alba, and quantification and qualification of PAs benefits based on Protected Areas Benefits Assessment Tool + (PA-BAT+) in six sites: Zlatibor, Golija, Tara, &#272;erdap, Stara planina, and Fru&#353;ka gora. Zlatibor, Golija, and Stara planina are protected as a Nature Park &#8211; protected areas of international, national, i.e., exceptional importance Category I (first) in accordance with the Law on Nature Protection ("Off. Gazette of RS", No. 36/2009, 88/2010 , 91/2010 and 14/2016). By the decision of the UNESCO commission within the MAB program in 2001, Golija was declared as Biosphere Reserve &#8221;Golija - Studenica&#8221;. Tara, &#272;erdap, and Fru&#353;ka gora are protected as National Parks &#8211; protected area of international, national, i.e., exceptional importance Category I (first) in accordance with the Law on National Parks ("Off. Gazette of RS", No. 39/1993, 44/1993-correction, 53/1993, 67/1993, 48/1994, 101/2005 and 36/2009). According to categorization of the IUCN Zlatibor, Golija, and Stara planina are classified in Category V, while Tara, &#272;erdap, and Fru&#353;ka gora are classified in Category II. Based on heavy metals content in soils and needles, different interventions in managed ecosystems are proposed.</p>

The Internet of Things (IoT) is integrated and applied in various domains such as Smart Home, Healthcare, Industrial IoT (IIoT), and others. IoT allows physical objects to be able to create, receive and exchange huge amounts of data at any time. The goal of such applications is to automate physical objects that will be able to function without human intervention or with minimal intervention depending on the purpose of the application. Because all domains involved involve the use of sensitive data and that data is usually transmitted over insecure channels, security and privacy issues arise due to potential cyber-attacks. This paper presents various IoT security techniques as well as the main security goals and characteristics. Three types of authentication schemes, one-factor, two-factor, and threefactor are then explained. Since authentication is one of the most important security goals, a special focus is on two-factor authentication schemes.

Bosnia and Herzegovina is among ten countries in the world with the highest mortality rate due to COVID-19 infection. Lack of lockdown, open borders, high mortality rate, no herd immunity, no vaccination plan, and strong domestic anti-vaccination movement present serious COVID-19 concerns in Bosnia and Herzegovina. In such circumstances, we set out to study if the population is willing to receive the vaccine. A cross-sectional study was conducted among 10,471 adults in Bosnia and Herzegovina to assess the attitude of participants toward COVID-19 vaccination. Using a logistic regression model, we assessed the associations of sociodemographic characteristics with vaccine rejection, reasons for vaccine hesitancy, preferred vaccine manufacturer, and information sources. Surprisingly, only 25.7% of respondents indicated they would like to get a COVID-19 vaccine, while 74.3% of respondents were either hesitant or completely rejected vaccination. The vaccine acceptance increased with increasing age, education, and income level. Major motivation of pro-vaccination behaviour was intention to achieve collective immunity (30.1%), while the leading incentive for vaccine refusal was deficiency of clinical data (30.2%). The Pfizer-BioNTech vaccine is shown to be eightfold more preferred vaccine compared to the other manufacturers. For the first time, vaccine acceptance among health care professionals has been reported, where only 39.4% of healthcare professionals expressed willingness to get vaccinated. With the high share of the population unwilling to vaccinate, governmental impotence in securing the vaccines supplies, combined with the lack of any lockdown measures suggests that Bosnia and Herzegovina is unlikely to put COVID-19 pandemic under control in near future.

<p>Since InSight (the Interior Exploration using Geodesy and Heat Transport) landed 26 months ago and deployed an ultra sensitive broadband seismometer(SEIS) on the surface of Mars, around 500 seismic events of diverse variety have been detected, making it possible to directly analyze the subsurface properties of Mars for the very first time. One of the primary goals of the mission is to retrieve the crustal structure below the landing site. Current estimates differ by more than 100% for the average crustal thickness. Since data from orbital gravity measurementsprovide information on relative variations of crustal thickness but not absolute values, this landing site measurement could serve as a tie point to retrieve global crustal structure models. To do so, we propose using a joint inversion of receiver functions and apparent incidence angles, which contain information on absolute S-wave velocities of the subsurface. Since receiver function inversions suffer from a velocity depth trade-off, we in addition exploit a simple relation which defines apparent S-wave velocity as a function of observed apparent P-wave incidence angles to constrain the parameter space. Finally we use the Neighbourhood Algorithm for the inversion of a suitable joint objective function. The resulting ensemble of models is then used to derive the full uncertainty estimates for each model parameter. Before its application on data from InSight mission, we successfully tested the method on Mars synthetics and terrestrial data from various geological settings using both single and multiple events. Using the same method, we have previously been able to constrain the S-wave velocity and depth for the first inter-crustal layer of Mars between 1.7 to 2.1 km/s and 8 to 11 km, respectively. Here we present the results of applying this technique on our selected data set from the InSight mission. Results show that the data can be explained equally well by models with 2 or 3 crustal layers with constant velocities. Due to the limited data set it is difficult to resolve the ambiguity of this bi-modal solution. We therefore investigate information theoretic statistical tests as a model selection criteria and discuss their relevance and implications in seismological framework.</p><div></div><div></div><div></div>