Background Because of the COVID-19 pandemic, people were recommended to implement new health behaviors into their daily routines to prevent the viral spread. The aim of this study was to investigate whether specific health behaviors, such as wearing face masks, taking immunity boosters and visiting risky places were associated with a higher level of stress due to COVID-19 in the general adult population. Method This cross-sectional study was conducted from September 1, 2020 to October 1, 2021 in eight randomly chosen cities of two Serbian speaking countries (Republic of Serbia and Republic of Srpska - Bosnia and Herzegovina). Participants filled a socio-epidemiologic questionnaire, COVID Stress Scales (CSS) and the Perceived Stress Scale (PSS). Results The study included 2,301 participants with an average age of 36.72 ± 13.82 years of whom 54.9% were female (p = 0.001). Most participants were healthy, highly educated, employed, married, non-smokers and consumed alcohol. The mean total CSS score was 32.7 ± 23.8 out of 144, suggesting a relatively low stress due to COVID-19. The mean PSS was 19.43 ± 5.05 out of 40 indicating slightly increased level of general stress. Participants who reported higher CSS scores were more likely to wear face masks, use immunity boosters, go to cafes and clubs, have chronic illnesses, have suspicious, but not proven contact with COVID-19 positive people, and use multiple sources of information about COVID-19. Conclusion Few participants experienced high levels of stress due to COVID-19. People who used face masks, immunity boosters and visited risky places reported a higher level of stress during the pandemic. Supplementary Information The online version contains supplementary material available at 10.1186/s41043-025-00833-2.

Realistically modeling interactions between road users — like those between drivers or between drivers and pedestrians — within experimental settings come with pragmatic challenges. Due to practical constraints, research typically focuses on a limited subset of potential scenarios, raising questions about the scalability and generalizability of findings about interactions to untested scenarios. Here, we aim to tackle this by laying the methodological groundwork for defining representative scenarios for dyadic (two-actor) interactions that can be analyzed individually. This paper introduces a conceptual guide for operationalizing controlled dyadic traffic interaction studies, developed through extensive interdisciplinary brainstorming to bridge theoretical models and practical experimental design. It elucidates critical trade-offs in scenario selection, interaction approaches, measurement strategies, and timing coordination, thereby enhancing reproducibility and clarity for future traffic interaction research and streamlining the design process. The methodologies and insights we provide aim to enhance the accessibility and quality of traffic interaction research, offering a guide that aids researchers in setting up studies and ensures clarity and reproducibility in reporting, bridging the gap between theoretical traffic interaction models and practical applications in controlled experiments, thereby contributing to advancements in human factors research on traffic management and safety.

This paper presents the most common traditional dovetail joints used in furniture making in past centuries, which are among the almost forgotten skills, practiced today by only a relatively small number of restoration workshops. The paper deals with the basic joints used in the restoration of furniture and traditional woodworking (houses, churches, temples, etc.) for the purpose of protecting rich cultural heritage. The history of furniture making is also the history of the art of making joints. In restoration, knowledge of these joints facilitates and accelerates the dating of furniture pieces to be restored. For this reason, the aim of this paper is to present an overview of the use of dovetail joints. The decorative effect of these joints and their constructive ingenuity certainly deserve more attention and research. A historical review of the development of traditional dovetail corner joints demonstrates the importance of the skill of crafting joints that stand the test of time and survive to this day. With the development of art, the joints became more and more decorative, but also of higher quality to withstand the various stresses that occur when furniture is used. Practical examples of making traditional dovetail corner joints are used to show how important it is to preserve the craftsmanship of these traditional joints from being forgotten, which is necessary when restoring furniture and other historical structural components made of wood such as ceilings, floors and altars.

This study aimed to determine the levels of Cd, Cr and Pb in basil (Ocimum basilicum L.) cultivated on artificially contaminated substrates and to assess their potential harmful effects on human health via the calculation of the target hazard quotients (THQ). A pot experiment was performed in a completely randomized design for each tested heavy metal. It included four contamination treatments (0, 20, 50 and 100 mg kg-1 for Cd, and 0, 100, 250 and 500 mg kg-1 for Cr and Pb). Concentrations of Cd, Cr and Pb in plants samples were  determined by atomic absorbtion spectroscopy. The results of this study showed that the concentrations of Cd, Cr and Pb were several times higher in roots than in the aboveground parts  of basil plants regardless of contamination levels. These are desirable results because only aboveground parts of basil  are used for medicinal purposes or consumption. The THQ values for Cd, Cr and Pb observed in this study were lower than 1 regardless of contamination levels, indicating that the consumption of basil from the study site (up to 10 g per day) does not pose a risk to human health from the point of view of heavy metal investigated.

Recent advancements in unmanned aerial vehicle (UAV) technology have opened new avenues for dynamic data collection in challenging environments, such as sports fields during fast-paced sports action. For the purposes of monitoring sport events for dangerous injuries, we envision a coordinated UAV fleet designed to capture high-quality, multi-view video footage of collision events in real-time. The extracted video data is crucial for analyzing athletes' motions and investigating the probability of sports-related traumatic brain injuries (TBI) during impacts. This research implemented a UAV fleet system on the NetLogo platform, utilizing custom collision detection algorithms to compare against traditional TV-coverage strategies. Our system supports decentralized data capture and autonomous processing, providing resilience in the rapidly evolving dynamics of sports collisions. The collaboration algorithm integrates both shared and local data to generate multi-step analyses aimed at determining the efficacy of custom methods in enhancing the accuracy of TBI prediction models. Missions are simulated in real-time within a two-dimensional model, focusing on the strategic capture of collision events that could lead to TBI, while considering operational constraints such as rapid UAV maneuvering and optimal positioning. Preliminary results from the NetLogo simulations suggest that custom collision detection methods offer superior performance over standard TV-coverage strategies by enabling more precise and timely data capture. This comparative analysis highlights the advantages of tailored algorithmic approaches in critical sports safety applications.

Food waste is a pressing global problem with significant environmental, economic and social impacts. This review examines the state of food waste management in Serbia and contextualizes the challenges and opportunities in a global and EU framework. In the Republic of Serbia, an estimated 247,000 tons of food is wasted annually, indicating critical gaps in waste management infrastructure, consumer awareness and missing legislation. While existing policies address general waste management, there is a lack of targeted measures for food waste prevention and resource recovery. The overview recommends aligning Serbian policy with an EU legislative frame, introducing extended producer responsibility and promoting public–private cooperation to improve food donation and recycling. This is the first comprehensive study specifically addressing food waste management in Serbia and assessing its compliance with European and global best practices. By comparing Serbia’s current status with established international models, this paper identifies critical gaps and proposes actionable strategies to improve the efficiency and sustainability of the food waste management system in Serbia. These include investment in infrastructure, public awareness campaigns and the use of innovative digital tools to reduce waste and support a circular economy.

The requisition for maintainable constructions has been greatly raising over the last several years. To fulfil the maintainability necessities of a construction, decisions or changes must be done to a construction in the course of the preconstruction and design steps. This can be plausible utilizing building information modelling. To indicate the utilize of building information modelling in maintainable planning, an example nursing-house is received for modelling research. The energy efficiency of nursing-home is analysed utilizing Autodesk Revit and Green Building Studio simulation which contained different characteristics such as annual heating and cooling loads, annual energy usage. Through using the utilize of different building, insulation and roof materials in the nursing-home modelling, the nursing-home modelling is changed into a greener construction modelling. In addition, the effects of using green walls on the facade of the building on the energy performance were analysed. Utilizing simulation, the utilize of non-natural sources can be dramatically decreased through substituting for them with the utilize of sustainable natural sources by that means energy saving. Building information modelling has substantiated to be effective in providing maintainability with alternative material’s assessment and earlier decision-making. Furthermore, this study employed an integrated new MCDM model to evaluate the performance of four natural stones for utilize in a nursing home setting.

To understand requirements for immunization via the oral mucosa, an in vitro model that recapitulates the physical barrier of the mouth, allows for quantification of antigen uptake and permeability and mounts an inflammatory response to antigen and adjuvant is needed. The physical structure of 4 models of the human oral mucosa was determined by histochemical staining and transepithelial electrical resistance (TEER) measurements. A TR146 based air-liquid interface (ALI) model most closely mimicked in vivo conditions. This was confirmed by validation studies using dextran and caffeine as diffusant molecules. Apparent permeability coefficients (Papp) of adenovirus (Ad) and adeno-associated virus (AAV) in this model were 4.3 × 10-13 and 2.2 × 10-10 respectively, while 100% of the total dose of H1N1 influenza remained in the epithelial layer. Sodium glycocholate and a hyperosmotic formulation improved the amount of Ad (p = 0.02) and AAV (p = 0.003) that entered the epithelium, respectively. Significant amounts of IL-6 (45.1 pg/mL), GM-CSF (94.7 pg/mL) and IFN-γ (4.3 pg/mL) were produced in response to influenza infection. Treatment with an AS03-like adjuvant induced production of IL-6 (34.9 pg/mL), TNF-∝ (43 pg/mL), GM-CSF (121.2 pg/mL) and IFN-γ (14.1 pg/mL). This highlights the contribution of differentiated epithelial cells to the immune response to vaccines and adjuvants.

Abstract The photosynthetic pigments, antioxidant properties, and heavy metal content in Reseda lutea, Epilobium dodonaei, and Gentianella ciliata were examined in response to stress in the open pit of an abandoned iron mine. The soils were shallow, alkaline, and severely deficient in phosphorus, potassium, and humus. Heavy metal concentrations in the rhizospheres followed the order Fe > Mn > Pb > Zn > Cu > Ni > Cr > Cd for all three species, with Cu, Zn, and Pb exceeding the limits established by Bosnian legislation. The results indicated that the bioelements Cu, Zn, and Mn were within permissible limits set by FAO/WHO. Epilobium dodonaei acted as a Cd accumulator. The highest content of photosynthetic pigments was observed in this species. Positive correlations were detected between Cr and total phenolics, Cr and total flavonoids in E. dodonaei, Pb and total phenolic acids in G. ciliata. Negative correlations were noted between Zn and total phenolic acids in R. lutea, and Fe and total phenolics in E. dodonaei. Increased total proline and DPPH concentrations were associated with heightened Fe levels in E. dodonaei. These findings suggest that the species analyzed employ distinct defense mechanisms, enabling them to effectively adapt to stress. NOVELTY STATEMENT Three abundant plant species with different responses to the stress conditions of the abandoned iron mine were the focus. The analyzed biochemical-physiological parameters for all studied species on alkaline soils and, in general, for Gentianella ciliata are presented for the first time.

Objective. We describe a rare case of satisfactory renal allograft function without immunosuppressive therapy following allogeneic hematopoietic stem cell transplantation (alloHSCT). Case Report. The patient was a 64-year-old male who had undergone a kidney transplant from a sibling donor in 2007. After 16 years, he required alloHSCT for acute myeloid leukemia (AML), with the same sibling serving as the donor for both transplants. HLA was a 50% match. Post-alloHSCT, immunosuppressive therapy was discontinued, and the renal allograft function remained stable. The patient later developed severe complications and succumbed to infection. Insights into the precise tolerance mechanisms were limited because laboratory evaluation for chimerism was not performed. Conclusion. There is potential for immunosuppressive-free renal allograft function after alloHSCT. This case underscores the significant risk of infection-related mortality. To achieve the best outcome, rigorous patient selection, tailored conditioning regimens, robust infection prevention strategies, and the possibility of combined transplantation for carefully selected patients are needed.

Background/Objectives: Squamous cell lung cancer (SCC) presents a significant treatment challenge due to its poor prognosis and limited therapeutic options. In many resource-limited countries, access to advanced molecular testing is often unavailable, making the identification of novel and reliable prognostic markers crucial for improving patient selection for systemic treatments. Methods: This single-center, retrospective study investigated the prognostic value of inflammatory biomarkers, including the systemic immune-inflammation index (SII), neutrophil-to-lymphocyte ratio (NLR), and platelet-to-lymphocyte ratio (PLR), in 134 patients diagnosed with SCC. Patients were stratified into groups based on optimal cut-off values determined by ROC analysis for each biomarker. Results: Elevated levels of the SII, NLR, and PLR were significantly associated with shorter overall survival in patients with SCC (all p < 0.05). Conclusions: These easily accessible and cost-effective laboratory parameters are particularly valuable in settings where molecular testing is not available, aiding in the identification of high-risk patients and optimizing treatment selection for chemotherapy.

Amiodarone, a class III antiarrhythmic drug, is widely used in both human and veterinary medicine to manage ventricular and supraventricular arrhythmias. While its efficacy in rhythm control is well-established, the drug is associated with significant adverse effects, affecting multiple organ systems. In humans, amiodarone-induced toxicity commonly involves the pulmonary, hepatic, thyroid, and dermatological systems, with pulmonary toxicity being one of the most severe complications. Acute hypersensitivity reactions, including anaphylaxis, are rare but have been reported, particularly in association with intravenous (IV) formulations containing polysorbate 80. In veterinary medicine, amiodarone is increasingly used to treat life-threatening arrhythmias in dogs, yet its safety profile is less extensively studied. Unlike humans, dogs appear to be more susceptible to immediate hypersensitivity reactions following IV administration, characterized by severe cutaneous signs, hypotension, and cardiovascular collapse. The present case report describes a 9-year-old Chihuahua that developed a rapid hypersensitivity reaction, including erythema, mucosal hyperemia, and facial edema, immediately after receiving IV amiodarone. The reaction resolved spontaneously within 15 minutes without requiring corticosteroid administration. Haematological and biochemical analyses showed a mildly decreased reticulocyte value, elevated neutrophil count, and increased ALT, while all other parameters were within reference ranges. A review of the literature suggests that excipients such as polysorbate 80 and benzyl alcohol may be primary contributors to amiodarone-induced anaphylaxis in dogs. Histamine-mediated responses, severe hypotension, and cardiovascular complications have been documented in both human and veterinary cases, with dogs displaying heightened sensitivity to IV administration. Additionally, while pulmonary and thyroid toxicity are well-recognized chronic effects in human patients, hepatic and gastrointestinal toxicities are more frequently observed in dogs. This case underscores the need for heightened awareness of amiodarone-induced hypersensitivity reactions in veterinary medicine, particularly in IV formulations. Pre-medication strategies, controlled infusion rates, and close monitoring are essential to mitigating the risk of life-threatening anaphylaxis in human and canine patients. Further research is needed to better understand species-specific differences in amiodarone metabolism and toxicity.