In filamentous fungi, gene silencing by RNA interference (RNAi) shapes many biological processes, including pathogenicity. Recently, fungal small RNAs (sRNAs) have been shown to act as effectors that disrupt gene activity in interacting plant hosts, thereby undermining their defence responses. We show here that the devastating mycotoxin-producing ascomycete Fusarium graminearum (Fg) utilizes DICER-like (DCL)-dependent sRNAs to target defence genes in two Poaceae hosts, barley (Hordeum vulgare Hv) and Brachypodium distachyon (Bd). We identified 104 Fg-sRNAs with sequence homology to host genes that were repressed during interactions of Fg and Hv, while they accumulated in plants infected by the DCL double knock-out (dKO) mutant PH1-dcl1/2. The strength of target gene expression correlated with the abundance of the corresponding Fg-sRNA. Specifically, the abundance of three tRNA-derived fragments (tRFs) targeting immunity-related Ethylene overproducer 1-like 1 (HvEOL1) and three Poaceae orthologues of Arabidopsis thaliana BRI1-associated receptor kinase 1 (HvBAK1, HvSERK2 and BdSERK2) was dependent on fungal DCL. Additionally, RNA-ligase-mediated Rapid Amplification of cDNA Ends (RLM-RACE) identified infection-specific degradation products for the three barley gene transcripts, consistent with the possibility that tRFs contribute to fungal virulence via targeted gene silencing. Significance Statement Fusarium graminearum is one of the most devastating fungal pathogens in cereals, while understanding the mechanisms of fungal pathogenesis is a prerequisite for developing efficient and environmentally friendly crop protection strategies. We show exploratory data suggesting that fungal small RNAs play a critical role in Fusarium virulence by suppressing plant immunity.

Motivated by the recent anomalies observed in semileptonic decays of B-mesons, the class of models denoted as"4321"is introduced and analysed. The importance of calculating next-to-leading-order (NLO) effects in performing precise compatibility tests of data with 4321 predictions is discussed. On the one hand, the coupling of the $U_1 \sim (\textbf{3},\textbf{1})_{2/3}$ leptoquark appearing in the spectrum to the third-generation fermions is large, giving rise to sizable NLO corrections in the relevant semileptonic operators. On the other hand, the new source of flavor violation is introduced with the vector-like fermions, resulting in the processes whose relevance can only be quantified at the loop order.

Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes coronavirus disease 2019 (COVID-19) whose pandemic was declared on March 11 2021 (1). Spectrum of COVID-19 clinical manifestations is very wide. Most patients report to ambulance with mild or moderate symptoms, but some of them rapidly develops acute respiratory distress syndrome (ARDS), respiratory failure, acute cardiac injury, multiple organ failure and death (2). Older age, diabetes mellitus and cardiovascular disease are reported as high predictors of morbidity and mortality. Aim: To determine correlation between diabetes mellitus and severity of clinical picture in patients with COVID-19. Methods: Current study involve retrospective analysis of 1513 patients with Real Time PCR confirmed COVID-19 hospitalized in Clinic for infectious disease, University Clinical Center, Sarajevo, Bosnia and Herzegovina, in a period of June 2020 to December 2020. Results: Among them 417 had previously diagnosed of diabetes mellitus. Results show that patients with diabetes mellitus are likely to require treatment in Intensive care unit, and oxygenic support with invasive ventilation. There was no statistically significant difference in outcome of the disease. Conclusion: Even this study didn’t find increased mortality in patients with COVID-19 and diabetes mellitus, further studies should be done to determine risk for patients with DM to develop severe form of disease.                   Peer Review History: Received 23 March 2021; Revised 17 April; Accepted  5 May, Available online 15 May 2021 UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency.  Received file:      Reviewer's Comments: Average Peer review marks at initial stage: 6.0/10 Average Peer review marks at publication stage: 7.5/10 Reviewer(s) detail: Dr. Branislav Rankovic, University of Kragujevac, Serbia, rankovic@kg.ac.rs Dr. Poualeu  Kamani  Sylviane  Laure, University of Dschang, Cameroon, poualeusylviane@yahoo.fr Similar Articles: THE RISKS AND ADVANTAGES OF ANTI-DIABETES THERAPY IN THE POSITIVE COVID-19 PATIENT

Bronchial asthma is a chronic disease of the airways, heterogeneous and of unknown etiology. In Brazil, decompensated bronchial asthma is the fourth leading cause of hospitalizations, with a higher incidence in the Northeast and Southeast regions, with descending mortality rates. The most common symptoms and signs are dyspnea, wheezing, retrosternal chest tightness and cough, resulting from the inflammatory process, with bronchial hyperreactivity and bronchoconstriction. This article aims to alert health professionals about the early diagnosis and treatment of exacerbated asthma in an emergency in the pandemic context of COVID-19, through this case report. Also dealing with the differential diagnosis in respiratory symptoms for COVID-19 with assessment of epidemiological risks and radiography, not very sensitive and specific for COVID-19, but widely available. This case report is about a 19-year-old Caucasian woman, single, born in Caruaru-PE, with complaints of low intensity chest discomfort, dyspnea and fatigue for three days, presenting exacerbation of symptoms in the last four hours, with previous diagnosis of asthma since childhood, currently with treatment interrupted due to fear of worsening for COVID-19 and waking up at night every day during the last month. After therapeutic conduct and clinical improvement, the patient was discharged with instructions on continuity of oral corticosteroids, return to bronchial asthma control therapy, need for follow-up in Primary Health Care (PHC) and symptoms of severity for bronchial asthma and COVID- 19 that indicate the need for emergency care.

Traffic management is a significantly difficult and demanding task. It is necessary to know the main parameters of road networks in order to adequately meet traffic management requirements. Through this paper, an integrated fuzzy model for ranking road sections based on four inputs and four outputs was developed. The goal was to determine the safety degree of the observed road sections by the methodology developed. The greatest contribution of the paper is reflected in the development of the improved fuzzy step-wise weight assessment ratio analysis (IMF SWARA) method and integration with the fuzzy measurement alternatives and ranking according to the compromise solution (fuzzy MARCOS) method. First, the data envelopment analysis (DEA) model was applied, showing that three road sections have a high traffic risk. After that, IMF SWARA was applied to determine the values of the weight coefficients of the criteria, and the fuzzy MARCOS method was used for the final ranking of the sections. The obtained results were verified through a three-phase sensitivity analysis with an emphasis on forming 40 new scenarios in which input values were simulated. The stability of the model was proven in all phases of sensitivity analysis.

To provide the quality required for its use, raw kaolin must be subjected to certain processing procedures like delamination which is applied to the layered structure materials. The aim of this research is to determine the possibility of performing delamination in the extruder and to estimate the effects of this process on the physico-chemical properties of kaolin. The results showed that delamination can be efficiently realized in the extruder by shearing densely packed layers under the influence of friction force and added Na2CO3 electrolyte. The crystal structure of the system was thus disrupted. This was confirmed by the Hinckley index (HI) decrease and by the dilatometric characteristics changes during heating. The particle size was reduced and, furthermore, new adsorption centers were formed, which led to an increase in the total cation exchange capacity (CEC). The plasticity of kaolin decreased with the increasing delamination degree, as well as the amount of water required for plastic processing, which is favorable in drying ceramic products.

We discuss neutrino magnetic moments as a way of constraining physics beyond the Standard Model. In fact, new physics at the TeV scale can easily generate observable neutrino magnetic moments, and there exists a multitude of ways of probing them. We highlight in particular direct dark matter detection experiments (which are sensitive to neutrino magnetic moments because of the predicted modifications to the solar neutrino scattering rate), stellar cooling, and cosmological constraints.

Lung-resident (LR) mesenchymal stem and stromal cells (MSCs) are key elements of the alveolar niche and fundamental regulators of homeostasis and regeneration. We interrogated their function during virus-induced lung injury using the highly prevalent respiratory syncytial virus (RSV) which causes severe outcomes in infants. We applied complementary approaches with primary pediatric LR-MSCs and a state-of-the-art model of human RSV infection in lamb. Remarkably, RSV-infection of pediatric LR-MSCs led to a robust activation, characterized by a strong antiviral and pro-inflammatory phenotype combined with mediators related to T cell function. In line with this, following in vivo infection, RSV invades and activates LR-MSCs, resulting in the expansion of the pulmonary MSC pool. Moreover, the global transcriptional response of LR-MSCs appears to follow RSV disease, switching from an early antiviral signature to repair mechanisms including differentiation, tissue remodeling, and angiogenesis. These findings demonstrate the involvement of LR-MSCs during virus-mediated acute lung injury and may have therapeutic implications. AUTHOR SUMMARY This work identifies a novel function of lung-resident MSCs during virus-induced acute lung injury. These findings contribute to the understanding of host response and lung repair mechanisms during a highly prevalent clinical situation and may have therapeutic implications.