Mechanical (hardness and adhesion) and electrical (sheet resistance) characteristics of electrolytically produced copper coatings have been investigated. Morphologies of Cu coatings produced galvanostatically at two current densities from the basic sulfate electrolyte and from an electrolyte containing levelling/brightening additives without and with application of ultrasound for the electrolyte stirring were characterized by SEM and AFM techniques. Mechanical characteristics were examined by Vickers microindentation using the Chen–Gao (C–G) composite hardness model, while electrical characteristics were examined by the four-point probe method. Application of ultrasound achieved benefits on both hardness and adhesion of the Cu coatings, thereby the use of both the larger current density and additive-free electrolyte improved these mechanical characteristics. The hardness of Cu coatings calculated according to the C–G model was in the 1.1844–1.2303 GPa range for fine-grained Cu coatings obtained from the sulfate electrolyte and in the 0.8572–1.1507 GPa range for smooth Cu coatings obtained from the electrolyte with additives. Analysis of the electrical characteristics of Cu coatings after an aging period of 4 years showed differences in the sheet resistance between the top and the bottom sides of the coating, which is attributed to the formation of a thin oxide layer on the coating surface area.

Background: An estimated 64.3 million people are living with heart failure worldwide. Functional MR in chronic HFrEF reflects primarily the severity of LV dysfunction and is not related to structural alterations of the mitral valvular apparatus. FMR in patients with HFrEF independently of the etiology of HFrEF and its underlying mechanisms, contributes to progression of the symptoms of HF and is independent predictor of worse clinical outcomes. Objective: The purpose of this study was to assess the severity of functional mitral regurgitation (FMR) and its clinical implications in patients with chronic heart failure with reduced ejection fraction (HFrEF). Methods: We enrolled 146 consecutive adult patients with CHF with reduced ejection fraction (HFrEF) who presented to outpatient clinics. All patients underwent clinical and physical examination. Baseline examination included medical history, detailed assessment of current medication, electrocardiogram recording, transthoracic echocardiogram and comorbidities. Heart failure with reduced ejection fraction was defined in line with the new guidelines as history of HF signs and symptoms as well as a LV ejection fraction (LVEF) below 40%. Cardiovascular risk factors were recorded according to the respective guidelines. FMR was defined and graded according to the ESC/EACTS Guidelines for the management of valvular heart disease. The extent of FMR was assessed at baseline and after a median follow-up period of 4 years in 146 consecutive HFrEF patients (left ventricular ejection fraction <40%). All of the patients received the heart failure (HF) medications in agreement with 2016 and 2021 ESC Guidelines for the diagnosis and treatment of acute and chronic heart failure. Major adverse cardiac events were defined as a composite of all-cause death and the need for admission for HF. Results: A total of 146 chronic HFrEF patients (mean age of 63±11 years, 62% male, mean LVEF of 25±11%) of which 19% patients had severe FMR at baseline, with a mean EROA of 31.4±2.7 mm2 and a mean Reg Vol of 45.9±5.3 ml. There was a significant interaction between FMR and NYHA functional class in predicting death or need for hospitalization, (P < 0.0001 for the interaction term FMR NYHA III-IV). During a median follow-up period of 4.2 (IQR) 3.1-5.8) years, the primary endpoint occurred in 52 (36%) patients (21 HF admissions, and 31 deaths). There was a strong graded association between the presence and degree of FMR and risk of death or admission (P <0.0001) at 4 years follow-up period. Regarding HF therapy, 129 patients (88%) received RAAS antagonists, 17 patients (12%) received ARNI, 86 patients (59%) received beta-blockers, 75 patients (51%) were treated with MRA. 31 patients (21%) underwent cardiac resynchronization therapy (CRT) with a response rate of 64%. 24 patients (16%) underwent ICD implantation. Conclusion: Guideline-directed medical therapy is the first-line treatment for chronic HF patients who also have FMR. After this first-line approach, surgical or MitraClip transcatheter therapy can be considered in patients with persistent severe and symptomatic FMR in order to improve symptoms, quality of life and functional status.

The book English in B&H: posh or casual? by Amna Brdarević-Čeljo and Vildana Dubravac makes a significant contribution to the sociolinguistic perspective on the status of English in the world and particularly in Bosnia and Herzegovina. Introducing readers to a wide array of sociolinguistic topics, the authors focus on the variability of English among its users and the prevalent impact of two most prominent world Englishes, namely Standardised British and Standardised American English. The theoretical discussion is further strengthened by research into Bosnian EFL users’ and EL teachers’ preference for one or the other variety in different domains of language use. Taking into consideration both perspectives, the authors ultimately put forward practical suggestions as to how to approach variability and consistency in language education in Bosnia and Herzegovina.

Background: Hypothyroidism occurs as a consequence of chronic autoimmune inflammation of the thyroid gland, which occurs due to the reduced function in the secretion of hormones FT3 and FT4 and requires replacement therapy for life. CoV-19 infection has shown many complications in all organic systems, during the acute phase of infection and in the post COVID period. Objectives: The aim of the study was a) to compare the frequency of patient visits for hypothyroidism and the average dose of levothyroxine in the SANASA polyclinic in the year before COVID pandemic, in the early 2019, with the frequency of patient visits during COVID infection in 2020 and 2021; b) to determine the incidence of hypothyroidism after the COVID 19 infection, the time of onset of hypothyroidism after acute phase of the disease, and the average dose of levothyroxine; and c) to monitor the incidence of subclinical hypothyroidism, which did not require substitution, before and after COVID 19 infection. Methods: In the SANASA polyclinic from the 2019 database we found 58 patients, at the age between 18-70 years, 53 women and 2 men with hypothyroidism and 2 female and 1 male patients with subclinical hypothyroidism. In 2020 there were a total of 89 patients, 73 women and 4 men with hypothyroidism, and 9 women and 3 men with subclinical hypothyroidism. In the 2021 there were 101 patients, 86 women and 7 men with hypothyroidism and 7 female and 1 male patients with subclinical hypothyroidism. Results: There was a significant difference in the number of patients with hypothyroidism and subclinical hypothyroidism during 2020 and 2021 in relation to 2019. The average dose of levothyroxine per patient did not differ statistically, comparing all three years, as well as comparing those who were ill, compared to patients who did not have COVID-19. There were diagnoses of post COVID subclinical hypothyroidism in 2020, as in 2021, with an average time of diagnosis of 2 months after infection for clinical hypothyroidism and 8 weeks for subclinical hypothyroidism. Conclusion: CoV-19 infection adversely affects thyroid tissue causing clinical hypothyroidism, requiring levothyroxine substitution as well as subclinical hypothyroidism which should be monitored.

Increasing drivers (i.e. pressures) on nature are present at the global scale, resulting in the upgrowth of the number of endangered species and habitats, as well as a decrease in the capacity of ecosystems to provide various benefits to the human population. Recent studies indicate that natural ecosystems are frequently unable to adequately and efficiently provide the flow of ecosystem services or nature’s contribution to people (i.e. NCP), emphasising that this negative trend will continue in the future. These trends can be linked with the existence of drivers or pressures on nature, which are numerous and have an increasing trend over time. Pressures on nature are all changes and disturbances in the functioning of biological processes and systems, followed by disruption of the ecosystem structure and the integration of the living world. In order to prevent the loss of biodiversity and ensure NCP, it is important to recognize and understand direct and indirect pressures on nature. In this sense, this paper presents the spatial distribution, intensity, trends and drivers of individual categories of pressures on nature in Bosnia and Herzegovina, as well as their combined effect. The presentation of pressures on nature is based on 16 broadly recognizable groups of ecosystems that include broad natural units (e.g. high mountain complexes), or a complex of ecological factors (e.g. dry and rocky meadows and pastures), the dominant type of use (e.g. agricultural areas) or the dominant type of pressures (e.g. urban areas) (Figures 2 and 3, Table 1). For the purposes of assessing the intensity of certain categories of pressures (direct and indirect) and their trends per ecosystem groups, an expert assessment procedure was carried out, using a structured research instrument, that enabled the analysis of experts’ views when it comes to the intensity and trend of certain categories of pressures for each ecosystem group (Tables 2 and 3). The results of this research indicate that the urban ecosystems, running water ecosystems and agricultural ecosystems are under the highest pressure, while the lowest pressures are associated with the underground and canyon ecosystems (Figures 4–13, 16). Direct pressures are generally rated as more intense than indirect pressures (Figures 14 and 15). For the majority of analysed direct and indirect pressures, similar effects and trends are confirmed as at the global level. According to the opinion of the experts, who participated in this research, overuse and pollution were assessed as the highest direct pressures on nature in Bosnia and Herzegovina, while institutional pressure was recognised as the highest indirect pressure. Keeping in mind that, according to their characteristics, institutional pressures are inseparable from the state authority and its institutions while, in the context of Bosnia and Herzegovina, they are inevitably linked to the socio-political organization and numerous criticisms of the (non)functioning of institutions. Groups of ecosystems with a significant share in total area (e.g. arable land, lowland and mountain deciduous forests and shrubs, etc.) are also characterized by high pressure with a moderate or increasing trend. Although competent institutions in Bosnia and Herzegovina participated in various international processes, systemic and institutional activities that would result in the application of good practices of the international initiatives regarding nature protection and sustainable use of natural resources are often missing. In that sense, the results of this research provide the decision-makers and the scientific community with scientifically based arguments and overall directions for the decision-making process and conducting future research in the field of natural resources and their sustainable use.

Lenticulostriate vasculopathy (LSV) is a relatively common fi nding in routine cranial ultrasound examination that has been associated with many infectious and non-infectious conditions. The aim of this review was to provide a better understanding of LSV ultrasound fi nding, as well as the need for further laboratory and imaging examinations in infants. The most of the published studies represented small series, with few prospective long-term studies involving the control groups. Authors have mostly found an association between LSV, especially higher-grade (although there is no universally accepted classifi cation) with congenital cytomegalovirus (CMV) infection, classifying those children as at risk for sensorineural hearing loss. In contrast, some authors pointed out that LSV could be found relatively often, and believe that isolated LSV, especially lower-grade, is not predictive for an unfavourable outcome and a long-term prognosis. Therefore, although 35 years have passed since the first publication of LSV, there is still no consensus among experts on the clinical signifi cance of isolated LSV, but caution is certainly needed given the fact that most infants with congenital CMV are asymptomatic.

Within the immune system, microRNAs (miRNAs) exert key regulatory functions. However, what are the mRNA targets regulated by miRNAs and how miRNAs are transcriptionally regulated themselves remain for the most part unknown. We found that in primary human memory T helper lymphocytes, miR-150 was the most abundantly expressed miRNA, and its expression decreased drastically upon activation, suggesting regulatory roles. Constitutive MIR150 gene expression required the RFX family of transcription factors, and its activation-induced down-regulation was linked to their reduced expression. By performing miRNA pull-down and sequencing experiments, we identified PDGFA-associated protein 1 (PDAP1) as one main target of miR-150 in human T lymphocytes. PDAP1 acted as an RNA-binding protein (RBP), and its CRISPR/Cas-9–mediated deletion revealed that it prominently contributed to the regulation of T-cell proliferation. Overall, using an integrated approach involving quantitative analysis, unbiased genomics, and genome editing, we identified RFX factors, miR-150, and the PDAP1 RBP as the components of a regulatory axis that restrains proliferation of primary human T lymphocytes.

In the scope of the genetic, morphological and phenological research of the genus Tilia in Bosnia and Herzegovina, with control populations in Germany, multibracteate silver-leaved linden (T. tomentosa Moench.) was discovered in Mostar. In addition to the usual primary bract, there are also bracts of the second and the third order, which as phenomenon is, to our knowledge, new for the science. Since the bracts vary in size, as well as in shape, and additional bracts do not appear on all the sampled trees, the task in the future is to further investigate this phenomenon.