Kampus Sveučilišta Jurja Dobrile u Puli karakteriziraju istaknute arhitektonske vrijednosti i vrijednosti otvorenog prostora. Otvoreni prostori čine više od 50% površine kampusa i uvelike određuju njegovu estetiku. Kartiranje dendroflore i kategorizacija zelenih površina kampusa provedeno je s ciljem pružanja temeljnih znanja za učinkovitije i isplativije upravljanje zelenim površinama kampusa. Poticanje zelenih otvorenih površina napravili su studenti Pejzažne arhitekture na Univerzitetu u Sarajevu – Šumarski fakultet tokom međunarodne studentske radionice CAMPULA u listopadu 2019. Studija je rezultirala jasnim informacijama o funkcionalnim svojstvima zelenih elemenata u Kampusu. Na području kampusa kartirana je ukupno 301 jedinka (203 zimzelena stabla, 80 listopadnih stabala, 9 zimzelenih grmova, 6 listopadnih grmova i 3 zimzelene palme) . Ukupno su kartirana 32 taksona (20 zimzelenih i 12 listopadnih). Poboljšano upravljanje zelenim otvorenim prostorom pulskog kampusa može rezultirati iznimno vrijednim ekološkim, društvenim i estetskim akademskim zelenim prostorom.

The efficient development and utilisation of magnetic nanoparticles (MNPs) for applications in enhanced biosensing relies on the use of magnetisation dynamics, which are primarily governed by the time-dependent motion of the magnetisation due to externally applied magnetic fields. An accurate description of the physics involved is complex and not yet fully understood, especially in the frequency range where Néel and Brownian relaxation processes compete. However, even though it is well known that non-zero, non-static local fields significantly influence these magnetisation dynamics, the modelling of magnetic dynamics for MNPs often uses zero-field dynamics or a static Langevin approach. In this paper, we developed an approximation to model and evaluate its performance for MNPs exposed to a magnetic field with varying amplitude and frequency. This model was initially developed to predict superparamagnetic nanoparticle behaviour in differential magnetometry applications but it can also be applied to similar techniques such as magnetic particle imaging and frequency mixing. Our model was based upon the Fokker–Planck equations for the two relaxation mechanisms. The equations were solved through numerical approximation and they were then combined, while taking into account the particle size distribution and the respective anisotropy distribution. Our model was evaluated for Synomag®-D70, Synomag®-D50 and SHP-15, which resulted in an overall good agreement between measurement and simulation.

Many recent research has focused on graphic design and shape of the packaging, and their effects on the customer's decision-making process as two separate categories. Since the shape of the packaging along with the graphic design plays an important role in the visual appearance of the packaging and attracts the customer's attention, the main objective of this research was to find out which packaging shapes and graphic elements customers find most attractive, as well as to understand the general opinion of customers about the influence of packaging aesthetics on their decision-making process when purchasing a product. Methodology of this research was conducted in two stages, from which the first one included creating three different graphic design solutions combined with three shape categories, and three variations of added value, for the same food packaging. The second part of the research covered an online questionnaire, in which a group of 50 people participated. This questionnaire focused on three variables: graphic design of the packaging, packaging shape and it’s added value. These variables were evaluated using the Likert scale. Through the questionnaire, participants were asked to answer general questions about the impact of the packaging visual appearance and added value, on their decision-making process when buying a product, as well as to choose the most appealing packaging between the given options. The research results showed that customers preferred non-standard packaging shapes and simple graphic design, as well as packaging with added value. The results of this research prove that the aesthetics of packaging has an impact on the customer's perception of a product, which means that packaging plays an important role in product marketing.

Abstract – Traffic safety should always be prioritized in all traffic systems. As the railway is a high-level complex technical and technological system, many factors affect its safety. Two factors that are the subject of research in this paper are train stopping distance and overlap. Keywords – Railway Traffic Safety, Train Stopping Distance, Overlap.

Background: Invasive ductal cancer (IDC) represents about 75% of all breast malignancies. There are many breast cancer prognostic factors, but the ones that have the most impact on the survival rates in advanced breast cancer are tumor size and regional lymph node involvement. Axillary lymph node dissection (ALND) has particularly important and undoubtful role in current surgical options for breast cancer treatment. With the introduction of sentinel lymph node biopsy (SLNB) for breast cancer patients it was possible to identify those to whom regional spread of the disease did not occur at the time of surgery, and thus spare them an unnecessary ALND procedure. Objective: To determine the rate of sentinel lymph node (SLN) detection using only methylene blue dye as a mapping agent, as well as to correlate the number of positive SLNs with the number of positive non-sentinel lymph nodes (non-SLNs). Methods: The study represents a prospective study that included 50 female patients with histologically confirmed invasive ductal carcinoma (IDC) who underwent SLNB using only methylene blue dye as the mapping agent, while the detection and harvest of SNL was done by visual control only. All patients also underwent an obligatory complete ALND, which was as that time the institutional oncological protocol for surgical treatment of histologically confirmed IDC. The final data such as tumor size, SLN and non-SLN status were obtained by further analysis of pathohistological reports from tumor biopsy and other surgical specimens. Results: The accuracy rate of SLN detection was 98%. The number of detected SLN was in the range of 1 to 6, with an average of 2 for each patient. The number of positive SLN was in significant correlation with the number of tumor-affected non-SNL (p<0,001). Further analysis showed that for each increase in the number of positive SLN by 1, the risk of positive non-SLN increased 6-fold, OR=6,22 (p<0,001). Conclusion: Use of methylene blue dye as a sole mapping agent when performing SLNB in patients with IDC is a reliable and effective method that can be safely implemented in medical institutions that lack availability of nuclear medicine services or significant monetary funds.

Abstract Extended-spectrum β-lactamases (ESBLs) hydrolyse extended-spectrum cephalosporins (ESC) and aztreonam. As ESBL-producing organisms have been identified in food producing animals, the aim of our study was to detect and analyse such Escherichia coli isolates from poultry. Antibiotic susceptibility of the isolates was determined with disk-diffusion and broth microdilution methods. ESBLs were detected with the double-disk synergy and inhibitor-based test with clavulanic acid. The transferability of cefotaxime resistance was determined with conjugation experiments, and genes encoding ESBLs, plasmid-mediated AmpC β-lactamases, and quinolone resistance determinants identified by polymerase chain reaction. The study included 108 faecal samples (cloacal swabs) from 25 different poultry farms in the Zenica-Doboj Canton, Bosnia and Herzegovina. Of these, 75 (69.4 %) were positive for E. coli, of which 27 were resistant to cefotaxime, amoxicillin, cefazoline, and cefriaxone, and susceptible to imipenem, meropenem, ertapenem, and amikacin. All 27 cefotaxime-resistant isolates were positive in double-disk synergy and combined disk tests. Eighteen isolates transferred cefotaxime resistance to E. coli recipient. Twenty-one isolates were positive for the blaCTX-M-1 cluster genes and seven for blaCTX-M-15. Fourteen were positive for the blaTEM genes. The most frequent plasmid incompatibility group was IncFIB, whereas IncFIA and Inc HI1 were present in only a few isolates. Two different sequence types (STs) were identified: ST117 and ST155. The emergence of ESBL-producing E. coli in farm animals presents a public health threat, as they can colonise the intestine and cause infections in humans.

Background: Non-alcoholic fatty liver disease (NAFLD) is an increasingly common cause of chronic liver disease and is becoming a major public health problem. NAFLD has been recognized as a hepatic manifestation of metabolic syndrome, associated with systemic diseases such as cardiovascular disease (CVD) and chronic kidney disease (CKD). Objective: The aim of this study was to examine the role of serum LFT parameters and renal function parameters as predictors of unmanifested liver disease. Methods: In this study, the presence of possible liver disease detected by biochemical parameters and confirmed by Transient Liver Elastography (TE) in a group of patients with different stages of chronic kidney disease (CKD) was investigated. Patients with various stages of CKD were divided into five subgroups regarding aetiology: nephroangiosclerosis, diabetic nephropathy, glomerulonephritis and pyelonephritis, autoimmune kidney disease, and polycystic and another morphological kidney disease. Liver stiffness was used to quantify liver fibrosis while Controlled attenuation parameter (CAP) was used to quantify liver steatosis. Functional liver tests and biochemical parameters of kidney function were measured in all patients. Results: Statistical analysis used in this study was a decision tree as a predictive model to map observed variables resulting in the conclusion about outcomes. The application of existing laboratory parameters, in combination with other parameters in presence of the defined etiological factors of kidneys diseases, indicate development of hepatic diseases. Higher values of phosphorus and low values of ferritin in patients with autoimmune kidney disease, and polycystic and another morphological kidney disease, expresses steatosis of the hepatic parenchyma. Conclusion: In contrary, low values of phosphorus and higher values of ferritin in patients with nephroangiosclerosis, diabetic nephropathy, glomerulonephritis and pyelonephritis, are in a favour steatosis of the hepatic parenchyma. Serum values of phosphorus and ferritin are valuable predictors of the liver disease in patients with end-stage kidney diseases of different aetiology.

Cotoneaster integerrimus represents a multiploid and facultative apomictic system of widely distributed mountain populations. We used flow cytometry to determine genome size, ploidy level, and reproduction mode variation of the Balkan populations, supplemented by analysis of nuclear microsatellites in order to address: (i) geographic distribution and variation of cytotypes among the populations; (ii) variation of reproduction mode and the frequency of sexuality; (iii) pathways of endosperm formation among the sampled polyploids and their endosperm balance requirements; (iv) genotypic diversity and geographic distribution of clonal lineages of polyploids. The prevalence of apomictic tetraploid cytotype followed by sexual diploids and extremely rare triploids was demonstrated. This prevalence of tetraploids affected the populations’ structure composed from clonal genotypes with varying proportions. The co-occurrence of diploids and tetraploids generated higher cytotype, reproductive mode, and genotypic diversity, but mixed-ploidy sites were extremely rare. The endosperm imbalance facilitates the development and the occurrence of intermediate triploids in mixed-ploidy populations, but also different tetraploid lineages elsewhere with unbalanced endosperm. All these results showed that the South European populations of C. integerrimus have higher levels of cytotype and reproductive diversity compared to the Central European ones. Therefore, the South European populations can be considered as a potential reservoir of regional and global diversity for this species.

The main goal of this paper is to determine the level of independence in performing daily activities in children and youth with cerebral palsy who are included in the regular education and rehabilitation program and to see their progress in relation to children and youth with cerebral palsy who are occasionally included or not included in the education and rehabilitation program. The research was conducted in the Center for Children with Multiple Disabilities "Koraci Nade (eng. Steps of Hope)" in Tuzla as well as in practice with the mobile team of the Centre. Total of 40 respondents were included. Respondents were divided into two groups: respondents who were included in the program and respondents who were occasionally or not at all included in the program. The results of the research indicate that the respondents who were included in the regular program of education and rehabilitation achieve better results in performing activities of everyday life in relation to the respondents who are occasionally or not at all included in the program. Key words: cerebral palsy, motor functioning, daily life activities, treatment

Abstract The electricity supplier, having direct contact with end consumers, has significant potential for contributing to the achievement of country’s strategic goals related to increasing the share of electricity production from renewable energy sources and promoting efficient use of electricity. This paper presents the concept of conducted analyses of energy efficiency at the customers of JP Elektroprivreda BH d.d. Sarajevo for the purpose of identifying consumers where it would be useful to apply some of the energy efficiency measures and/or peak load management, all for reducing electricity costs. The results of the analysis are presented in the paper. Special attention is given to the techno-economic analysis of the application of measures used to improve the energy efficiency. The results of the analyses show that the shortest payback period is achievable by adequate waste heat recovery in compressors and refrigeration systems, reactive power compensation, control of motor drives of pumps and fans, and the replacement of motors with a higher efficiency class.

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Background: Autism spectrum disorder (ASD) is a developmental disability, which is a biologically based neurodevelopmental disorder that affects a child’s social interaction and communication skills. Core deficits are identified in two domains: social communication/interaction and restrictive, repetitive patterns of behavior. Children and youth with ASD have service needs in behavioral, educational, health, leisure, family support, and other areas. Autism is a set of heterogeneous neurodevelopmental conditions, characterized by inability to acquire social skills, repetitive behaviors and failure of speech and nonverbal communication development. Objective: To examine frequency, correlation and predictivity of communication and language skills of autistic spectrum disorders (ASD) children and their parents’ emotions. Methods: The sample consisted of 80 participants who are parents of children with ASD. The study was performed using a questionnaire made out for this research. Three groups of variables were analyzed: parental emotions variables; child’s communication variables, child’s language variables. Statistical analysis was performed by SPSS, and included basic statistical parameters and multiple regression analysis. Results: The most common ways of communicate for children with ASD are: Allowing to cuddle; Recognizes familiar faces; and Makes it known when he needs help or wants an item. The most common language forms of children ASD are: Following simple instructions; Understanding connected words/phrases; Pronouncing single words; and Looking at when called by name. Parents of children with ASD expressed the greatest agreement with the following statements: “My child is more demanding than most other children”; “It seems to me that my child is very sensitive and easily upset”; “My child can’t do much of what he’s expected to know to do”, and “I often lack energy.” Conclusion: Parents of children with ASD proved that their children have significant delays in communication and language. Developed communication and language skills of the child with ASD are valid predictors of parents’ emotions/attitudes. Speech and language therapy work focused on developing the ASD child’s communication and language skills can be expected to improve parents’ emotions/attitudes.

Healthy and controlled immune response in COVID-19 is crucial for mild forms of the disease. Although CD8+ T cells play important role in this response, there is still a lack of studies showing the gene expression profiles in those cells at the beginning of the disease as potential predictors of more severe forms after the first week. We investigated a proportion of different subpopulations of CD8+ T cells and their gene expression patterns for cytotoxic proteins (perforin-1 (PRF1), granulysin (GNLY), granzyme B (GZMB), granzyme A (GZMA), granzyme K (GZMK)), cytokine interferon-γ (IFN-γ), and apoptotic protein Fas ligand (FASL) in CD8+ T cells from peripheral blood in first weeks of SARS-CoV-2 infection. Sixteen COVID-19 patients and nine healthy controls were included. The absolute counts of total lymphocytes (p = 0.007), CD3+ (p = 0.05), and CD8+ T cells (p = 0.01) in COVID-19 patients were significantly decreased compared to healthy controls. In COVID-19 patients in CD8+ T cell compartment, we observed lower frequency effector memory 1 (EM1) (p = 0.06) and effector memory 4 (EM4) (p < 0.001) CD8+ T cells. Higher mRNA expression of PRF1 (p = 0.05) and lower mRNA expression of FASL (p = 0.05) at the fifth day of the disease were found in COVID-19 patients compared to healthy controls. mRNA expression of PRF1 (p < 0.001) and IFN-γ (p < 0.001) was significantly downregulated in the first week of disease in COVID-19 patients who progressed to moderate and severe forms after the first week, compared to patients with mild symptoms during the entire disease course. GZMK (p < 0.01) and FASL (p < 0.01) mRNA expression was downregulated in all COVID-19 patients compared to healthy controls. Our results can lead to a better understanding of the inappropriate immune response of CD8+ T cells in SARS-CoV2 with the faster progression of the disease.

Abstract The main aim of this paper is to explore the factors determining Microfinance institutions (MFIs) self-sufficiency. The data on selected variables for this research were obtained from the public MIX Market Database and cover the year of 2017. The empirical model is constructed with application of a Principal Component Analysis (PCA) and Logistic regression analysis. Sample is consisted of 342 MFIs from all around the world, with 21 independent variables grouped into eight factors/components, and OSS (operational self-sufficiency) as dependent variable. The obtained results suggest that higher revenue and MFIs profitability combined with decrease of credit risk lead to higher probability of MFI to be self-sufficient. These results also confirm widespread belief that MFIs will not be able to achieve their social goals without achieving sustainable profitability. In addition, results also confirm importance of MFIs core mission as with increase in outreach, probability of MFIs achieving self-sustainability also increases.