Using the Design of Experiments methodology (Response-Surface Methodology and Derringer's Desirability Function), a simple, fast and robust RP-HPLC method was developed for the analysis of enrofloxacin (EFC), its impurity A (fluoroquinolonic acid, FQ) and impurity B (ciprofloxacin, CPX). Gradient elution of samples was performed on a Zorbax Eclipse XDB C18 column (150 × 4.6 mm, 3.5 μm) with a mobile phase consisting of 32 mM phosphate buffer pH 3.5 – methanol (0 min-19.6% methanol; 15.5 min-19.6% methanol; 29.5 min-80% methanol; 30 min-19.6% methanol; 35 min-19.6% methanol), delivered at a flow rate of 1.5 mL min−1, wavelength of detection 278 nm (for EFX and CFX) and 265 nm for FQ. A good linear response was achieved in the range 15–35 μg mL−1 (EFX) and LOQ-150% for impurities (CFX and FQ). Other validation parameters were also tested: precision, accuracy, sensitivity and robustness. The developed method was shown to be simple, practical and suitable for the analysis of EFC and its impurities (CPX, FQ) in veterinary drugs.

Time is a uniquely human yet culturally ubiquitous concept acquired over childhood and provides an underlying dimension for episodic memory and estimating durations. Because time, unlike distance, lacks a sensory representation, we hypothesized that subjects at different ages attribute different meanings to it when comparing durations; pre-kindergarten children compare the density of events, while adults use the concept of observer-independent absolute time. We asked groups of pre-kindergarteners, school-age children, and adults to compare the durations of an "eventful" and "uneventful" video, both 1-minute long but durations unknown to subjects. In addition, participants were asked to express the durations of both videos non-verbally with simple hand gestures. Statistical analysis has revealed highly polarized temporal biases in each group, where pre-kindergarteners estimated the duration of the eventful video as "longer." In contrast, the school-age group of children and adults claimed the same about the uneventful video. The tendency to represent temporal durations with a horizontal hand gesture was evident among all three groups, with an increasing prevalence with age. These results support the hypothesis that pre-kindergarten-age children use heuristics to estimate time, and they convert from availability to sampling heuristics between pre-kindergarten and school age.

Nanomaterials have been the focus of intensive development and research in the medical and industrial sectors over the past several decades. Some studies have found that these compounds can have a detrimental impact on living organisms, including their cellular components. Despite the obvious advantages of using nanomaterials in a wide range of applications, there is sometimes skepticism caused by the lack of substantial proof that evaluates potential toxicities. The interactions of nanoparticles (NPs) with cells of the immune system and their biomolecule pathways are an area of interest for researchers. It is possible to modify NPs so that they are not recognized by the immune system or so that they suppress or stimulate the immune system in a targeted manner. In this review, we look at the literature on nanomaterials for immunostimulation and immunosuppression and their impact on how changing the physicochemical features of the particles could alter their interactions with immune cells for the better or for the worse (immunotoxicity). We also look into whether the NPs have a unique or unexpected (but desired) effect on the immune system, and whether the surface grafting of polymers or surface coatings makes stealth nanomaterials that the immune system cannot find and get rid of.

ABSTRACT The research determines the gap (Great Decoupling) between labour productivity and workers’ compensation in the two blocks of EU countries (Western versus Eastern). The division of countries into two groups provides a basis further to determine whether the previous socio-economic and political evolutionary development of these countries blocks still has a significant impact on the functional distribution of national income, on the extent to which labour productivity growth is transmitted to workers. The results are heterogeneous. In the sample of highly developed Western EU countries where higher levels of labour productivity, as well as high levels of technological development, lead to an increase in labour productivity to be followed by a lower increase in workers’ compensation. On the sample of Eastern EU countries, results indicate different relationships and the strength of causality between productivity and labour compensation. Central-East EU countries had a more positive relationship between real workers’ compensation and labour productivity, compared to the Southeast Europe (Balkan) countries where an increase in workers’ compensation causes a reduction in labour productivity. The results also offer a solid basis for understanding wage/income/productivity relationships d for creating policies for a more efficient distribution of national income.

Southeast Asia has the most significant tropical peat/peat carbon storage area in the world, with Indonesia being the primary location for much of it. Anthropogenic changes to peatlands have resulted in a threat to these endangered ecosystems; policies that have favored industrial and elite interests above those of local communities have resulted in severe consequences for the environment and public health, not only in Indonesia and its region, but for the world community in terms of contributions to climate change. Decentralization has been seen as a means of sharing authority and accountability with lower government levels and providing additional opportunities for shared governance. Still, there is reason to question the means of these approaches and the results of such efforts. The research question is: What can be learned about the administration’s role in leading stakeholder involvement from the case of Indonesian peatland management? The authors utilize outcome additionality as a framework connected to the resilience of peatlands.

Abstract Jennings, J, Štaka, Z, Wundersitz, DW, Sullivan, CJ, Cousins, SD, Čustović, E, and Kingsley, MI. Position-specific running and technical demands during male elite-junior and elite-senior Australian rules football match-play. J Strength Cond Res 37(7): 1449–1455, 2023—The aim of this study was to compare position-specific running and technical demands of elite-junior and elite-senior Australian rules football match-play to better inform practice and assist transition between the levels. Global positioning system and technical involvement data were collated from 12 Victorian U18 male NAB League (n = 553) and 18 Australian Football League (n = 702) teams competing in their respective 2019 seasons. Players were grouped by position as nomadic, fixed, or ruck, and data subsets were used for specific analyses. Relative total distance (p = 0.635, trivial effect), high-speed running (HSR) distance (p = 0.433, trivial effect), acceleration efforts (p = 0.830, trivial effect), deceleration efforts (p = 0.983, trivial effect), and efforts at >150 m·min−1 (p = 0.229, trivial effect) and >200 m·min−1 (p = 0.962, trivial effect) did not differ between elite-junior and elite-senior match-play. Elite juniors covered less total and HSR distance during peak periods (5 seconds–10 minutes) of demand (p ≤ 0.022, small-moderate effects). Within both leagues, nomadic players had the greatest running demands followed by fixed position and then rucks. Relative disposals (p = 0.330, trivial effect) and possessions (p = 0.084, trivial effect) were comparable between the leagues. During peak periods (10 seconds to 2 minutes), elite juniors had less technical involvements than elite seniors (p ≤ 0.001, small effects). Although relative running demands and positional differences were comparable between the leagues, elite juniors perform less running, HSR, and technical involvements during peak periods when compared with elite seniors. Therefore, coaching staff in elite-senior clubs should maintain intensity while progressively increasing the volume of training that recently drafted players undertake when they have transitioned from elite-junior leagues.

High-quality academic publishing is built on rigorous peer review [...].

Abstract Background Small renal masses (SRMs) are defined as contrast-enhanced masses with a diameter of ≤4 cm, usually consistent with clinical stage T1a renal cell carcinoma (RCC). This study aimed to explore the histopathological features of a contemporary series of SRMs and investigate parameters that could predict their pathological nature, metastatic potential, and recurrence potential. Materials and methods Small renal masses were identified in 166 of 427 patients who underwent surgery for suspected RCC. The radical nephrectomy/partial nephrectomy ratio was 1:44, and autotransplantation was performed in a single case. Variables associated with metastatic recurrence of SRM were analyzed using χ2 and logistic regression models. Results Renal cell carcinoma was confirmed in 86% (n = 143) of cases, whereas benign tumors were present in 14% (n = 23) of cases. Seventeen percent of the RCC cases were high-grade (Fuhrman G3–4). Among SRMs with a diameter of >2 cm, 71% were malignant. The mean ± standard deviation diameter of the removed SRMs was 28 ± 12 mm, significantly higher in the malignant SRMs group (31 ± 8 vs. 24 ± 9 mm) (p = 0.005). During follow-up, local recurrence was identified in 4 patients versus new distant metastasis in 11 patients. Metastatic lesions were detected in the lungs (1.8%), bone (1.2%), distant (1.9%) and regional lymph nodes (1.2%), liver (0.6%), and multiple organs (2.4%). Patients with malignant SRMs were significantly older than those with benign tumors (p = 0.036). The multivariate analysis identified tumor size, Fuhrman grade, stage, nodal and distant metastasis status, tumor localization, and treatment modality as characteristics significant for tumor recurrence. Conclusions The majority of treated neoplasms were malignant, but their incidence was low among SRMs with a diameter of <2 cm. Our results emphasized the importance of histopathological features and treatment modalities in predicting malignant recurrence.

The SI4CARE project is a transnational project which aims to develop both strategy and action plans to improve health and social care in the Adriatic–Ionian region. Starting from a survey of the status quo, each partner has developed some pilots to support the development and monitoring of the policy actions. In particular, partner number three, the Municipality of Miglierina, designed and developed a pilot related to the use of wearable devices for monitoring elderly patients in rural areas. With the collaboration of the complex unity of primary care (UCCP) of the Reventino area, the pilot is based on the use of smart wearable devices to monitor some parameters of older adults after their vaccinations for flu and covid. This paper focused on the design and implementation of the system. It describes its application in the Municipality of Miglierina. Presentation of the results and a discussion of the strengths and weaknesses will be presented, in detail, in future work. Finally, the possibility of extending the experiment to other Adriatic–Ionian regions is addressed.

According to the WHO, as of January 2023, more than 850 million cases and over 6.6 million deaths from COVID-19 have been reported worldwide. Currently, the death rate has been reduced due to the decreased pathogenicity of new SARS-CoV-2 variants, but the major factor in the reduced death rates is the administration of more than 12.8 billion vaccine doses globally. While the COVID-19 vaccines are saving lives, serious side effects have been reported after vaccinations for several premature non-communicable diseases (NCDs). However, the reported adverse events are low in number. The scientific community must investigate the entire spectrum of COVID-19-vaccine-induced complications so that necessary safety measures can be taken, and current vaccines can be re-engineered to avoid or minimize their side effects. We describe in depth severe adverse events for premature metabolic, mental, and neurological disorders; cardiovascular, renal, and autoimmune diseases, and reproductive health issues detected after COVID-19 vaccinations and whether these are causal or incidental. In any case, it has become clear that the benefits of vaccinations outweigh the risks by a large margin. However, pre-existing conditions in vaccinated individuals need to be taken into account in the prevention and treatment of adverse events.

The number of female trainees is on the rise, and so too are the issues surrounding fertility and barriers to family planning. Over the past decade, studies of surgical residents across the United States have demonstrated a strong prevalence of fertility challenges. Strenuous training requirements have American female surgical trainees delaying pregnancy, having fewer children, and reporting increased rates of pregnancy complications, infertility, and use of assistive reproductive technology. Similarly, a survey of British surgical trainees showed over a quarter of trainees felt unsupported by their department during pregnancy, while almost a quarter of both of male and female trainees experienced difficulty in arranging parental leave. Our recent article investigated how our long training programmes are affecting Australasian surgical trainees’ ability to start families, particularly when compared with other specialities. While we acknowledge there are limitations to online surveys, the results of this survey of 1099 doctors demonstrated that surgeons, when compared with physicians and GPs, are experiencing greater challenges in balancing work and family, across multiple areas of fertility and child-caring. Surgical training occurs in the prime reproductive years and as a consequence, when many trainees finally complete their training, they are living the real-world consequences of reduced fertility because of their age. This highlights the critical need for a cultural change that’s becoming increasingly important as the surgical workforce demographic changes. Training bodies are in a prime position to provide solutions to the many obstacles surgical trainees face in family planning. There are several ways the colleges and hospitals can support a more equitable future for its members who want to pursue parenthood.

Elite female karatekas should have specific morphological characteristics suitable for their specializations. This study aimed to determine the differences in morphological characteristics and body composition of female karatekas according to different specializations. This study consisted of a total of 15 female karatekas divided according to specialization in kumite (fighting) and kata (form or movement pattern) disciplines. The subject sample included healthy, female senior karatekas, with no prior injuries divided into kumite (n-8, 22.75±4.65 years) and kata (n-7, 22.00±4.58 years) athletes. Morphological characteristics and body composition were evaluated by a battery of 11 variables: body height (BH), body mass (BM), triceps skinfold (TS), biceps skinfold (BiS), back skinfold (BS), abdominal skinfold (AS), upper leg skinfold (UlS), lower leg skinfold (LlS), body mass index (BMI), fat percentage (FP), and muscle mass percentages (MP). Based on the t-test for small independent samples, findings showed that female kata and kumite karatekas do not have different morphological characteristics. Although there was no difference in morphological characteristics, for more complete conclusions an analysis should be performed on a larger sample of elite female karate athletes.