Integrated fruit production (IFP) is an economical, high-quality fruit production which prioritizes ecologically acceptable means of production, which minimize side-effects aiming to increase environment conservation and human health. Following the market demands and increasing production standards, integrated production imposes itself, which is to enable lucrativeness, market competition and ecological acceptability of agricultural products. Introducing and implementing multi-criteria model of decision-making is based on DEXi method (multi-attribute analysis). This method makes selection of the most adequate fruit sort for initiating fruit production. This model of decision-making is based on opinions of experts from the Feld of integrated production. The main criterion in evaluating IFP according to DEXi and expert system are: economic, technological, ecological and socio-political. The result of multi-criteria expert system DEXi have shown that the plum fruit sort yields the best results according to integrated production concept and can be recommended as the first planting alternative. The second alternative recommends apple while the third one recommends pear.

Neste trabalho efetuamos um estudo sistematico das propriedades estruturais e eletronicas da hidroxiapatita (HA), atraves da teoria funcional de densidade (DFT) usando o codigo – CASTEP. Os resultados obtidos atraves da tecnica de aproximacao GGA – PBE demonstraram em termos das propriedades estruturais uma diferenca maxima de 13.4 % entre o valor teorico e experimental, enquanto o volume calculado com estes valores foi V = 529,58 A 3 .

AIM: The research was conducted by genotyping two Human Leukocyte Antigen (HLA) gene classes. The main objective of this research was to investigate distribution and frequency of the allelic groups, genotypes and haplotypes in the gene loci of HLA class I (HLA-A*, -B*, -C*) and HLA class II (HLA-DRB1*, -DQB1*) in patients included in the program of cadaveric renal transplantation. MATERIAL AND METHODS: Our study covered 186 blood samples of patients who are registered on the list for cadaveric renal transplantation in Federation of Bosnia and Herzegovina and included 59 control, healthy unrelated individuals. For the HLA typing, we have used three different methods: micro lymphocyte cytotoxicity test (MLCT), Polymerase Chain Reaction (PCR) – Sequence Specific Primers (SSP) and PCR – Sequence-Specific Oligonucleotides (SSO) or Luminex technology. All patients and cadaveric donors were tested using the three methods because the system is polymorphic. RESULTS: Analysis of the results of genotyping HLA class I gene loci identified dominant HLA-A*02, HLA-B*35, HLA-C*07 allelic groups. Analysis of the HLA class II gene loci genotyping showed that HLA-DRB1*11 and HLA-DQB1*03 loci had the highest incidence in HLA class II. CONCLUSION: Based on our results and previous research, there were no observed differences between allelic frequencies and genotypes of healthy people and people with ESRD. Differences between allelic groups occurred, but they were not statistically significant, except HLA-C*01 (p = 0.020).

There are differences with respect to the commonly isolated Malassezia species, not only between healthy individuals and the patients with various skin diseases, but also between different countries. We investigated the species composition of Malassezia microflora on the skin of patients with Malassezia-associated diseases and of healthy subjects (HS). Two hundred and fifty skin scrapings from patients with pityriasis versicolor (PV), seborrheic dermatitis (SD), atopic dermatitis (AD), psoriasis (PS), and healthy subjects (HS), fifty each, were inoculated into Sabouraud dextrose agar and into modified Dixon agar and identified using conventional culture-based methods. In PV and PS lesions, the most common species was M. globosa (62% and 52%, respectively), while M. restricta was predominant in SD lesions (28%). M. sympodialis was the most common species recovered from AD (52%) and healthy trunk skin (30%). Fewer cultures were positive for Malassezia growth in patients with AD than in patients with other skin conditions, and even in controls. Our data are in agreement with other studies and suggest that the pathogenic species of PV is M. globosa. The evidence that any given species is clinically important in the pathogenicity of SD, AD and PS is still lacking.