Pharmacognosy as a science of natural medicinal raw materials and drugs of natural origin is a science that protects nature and affects the survival and protection of man. From the earliest times, man was exploring everything that nature provides. With the development of science and technology, research into natural raw materials, as well as those with healing activity, was perfected. The obtained results could reliably be used in official pharmacopoeia, medicine and dentistry. With the development of computer technology, there has been more detailed information on the use of natural medicinal raw materials through Phytotherapy, which is increasingly being applied. All these studies indicate that pharmacognosic studies with the goal of using natural medicinal substances and medicines of natural origin must not be called alternative medicine, but it is the same as all other medicines that are only adapted to the nature of natural raw materials.

This study evaluates the genotoxic potential of two Ru(III) complexes with thiosemicarbazone based ligands. The complexes were tested for in vitro protective effect on chromosome aberrations in peripheral human lymphocytes using the cytokinesis block micronucleus (CBMN) assay at concentrations 1.5; 3.7 and 7.4 μg/mL. The cell culture treated with the tested complexes, at 3.7 μg/mL concentration, decreased a frequency of micronucleus for 37% and 32%, when compared with the control cell cultures. At concentration of 7.4 (1.5) μg/mL of this complexes exhibited slightly lower effect of micronucleus for 30% (35%) and 27% (29%), when compared with the control cell cultures.