Summary The apple (Malus domestica Borkh.) possesses a number of health-promoting properties. The main beneficial effects of apple fruits on human health are associated with the antioxidant properties of components synthesising in apple fruits during their growth and development. Phenolic compounds and flavonoids are such components that contribute significantly to the antioxidant capacity of fruits, thus enhancing their health properties. The purpose of this study is to compare the antioxidant properties (namely antioxidant capacity, ascorbic acid, total phenolic and flavonoid contents) of the ‘Idared’ and ‘Braeburn’ apple cultivars, grown under optimal growing conditions in the Goražde area. Ascorbic acid contents were estimated using the 2.6-dichlorophenolindophenol titration method, whereas total phenolics were determined using the Folin-Ciocalteu method. Furthermore, total flavonoids were determined using the aluminium chloride colorimetric assay, whereas the ferric reducing/antioxidant power assay was used to determine the total antioxidant capacity of the apple fruits examined. The average values of the ascorbic acid, the total phenolic and flavonoids contents, and the total antioxidant capacity of the ‘Idared’ apples under consideration were 8.09 mg 100 g−1, 34.56 mg gallic acid equivalents 100 g−1, 12.62 mg catechin equivalents 100 g−1 and 323.28 μmol Fe2+ 100 g−1of fresh weight, respectively. The average values of the total phenolic and flavonoids contents, as well as the antioxidant capacity of the ‘Braeburn’ apples examined were as follows: 8.89 mg 100 g−1, 33.4 mg gallic acid equivalents 100 g−1, 13.56 mg catechin equivalents 100 g−1 and 367.01 μmol Fe2+ 100 g−1 of fresh weight, respectively. The analyses performed indicate that the differences recorded in the parameter values between the apple cultivars ‘Idared’ and ‘Braeburn’ were statistically non-significant. A correlation data analysis confirmed that phenolic compounds are the carriers of the antioxidant activity of apples.

The aberrant DNA methylation plays a critical role in a number of different malignancies, including melanoma. DNA methylation is catalyzed by DNA methyltransferases (DNMTs), involved in methylation maintenance (DNMT1) and de novo DNA methylation (DNMT3A and DNMT3B). The current study investigated the association of genetic variants in the DNMT1 and DNMT3B with the clinicopathologic features and the clinical course of melanoma patients. In the present study, DNMT1 (rs2228612, rs2228611, and rs2114724) and DNMT3B (rs406193 and rs2424932) polymorphisms were examined in 123 melanoma patients. Single nucleotide polymorphisms were assessed using TaqMan SNPs Genotyping Assays according to the manufacturer's protocols. The carriers of the variant genotype of DNMT1 rs2228612 had poorer overall survival and recurrence-free survival, (P=0.000 and 0.000, respectively), and an increased risk for adverse outcome [hazard ratio (HR)=6.620, 95% confidence interval (CI): 2.214-19.791, P=0.001]. DNMT1 rs2228612 was also associated with ulceration (P=0.045), nodal status (P=0.030), progression (P=0. 007), and stage of disease (P=0.003). Univariate analysis indicated that tumor-infiltrating lymphocytes could be a marker of good prognosis in melanoma patients (HR=0.323, 95% CI: 0.127-0.855, P=0.025), whereas the genotype distribution of the DNMT3B rs406193 polymorphism correlated significantly with the presence of tumor-infiltrating lymphocytes (P=0.012). The multivariate analysis showed that the DNMT1 rs2228612 polymorphism (HR=12.126, 95% CI: 2.345-62.715, P=0.003) is an independent predictor of poor overall survival in melanoma patients. As expected, disease progression was also found to be an independent prognostic factor in melanoma patients (HR=37.888, 95% CI: 3.615-397.062, P=0.002). DNMT1 rs2228612 was found to be an independent predictor of poor overall survival in melanoma patients. DNMTs polymorphisms could serve as a potential target for novel therapeutic approaches.

Six Sigma is a version, a philosophy, a strategy and a set of tools for improvement of the quality services and processes. Till now, this method was mostly used in the world of manufacture. Telecommunication is the industry that is based on the service, where the customers are the main focus, and their needs very often seem unpredictable. In this work, a critical review had been given to the application of the Six Sigma methodology in the department of customers support of a telecom company. Through SWOT analysis, given is a review of the opportunities and challenges of this methodology, and also what is necessary to be changed for this methodology to have a purpose and application in one telecommunication company.

The effect of pH and initial concentration on the removal of Cu(II) and Ni(II) ions from their monocomponent and two-component aqueous solutions using Na2CO3 as a chemical precipitation agent was investigated in this paper. Monocomponent aqueous solutions  of Cu(II) and Ni(II)  ions with their initial concentrations of 50 and 500 mg/L and two-component aqueous solution with initial concentration of 500 mg/L were prepared. The precipitation was carried out by batch method at room temperature by stirring the solution at 300 rpm for 5 minutes, resulting in the formation of precipitates. The resulting precipitate was separated by filtration from the solution. The experiment proved that Na2CO3 is a good agent for removing Cu(II) and Ni(II) ions from their monocomponent water solutions of 50 and 500 mg/L concentration and two-component water solution with initial concentration of each metal 500 mg/L. The percentage of Cu(II) removal was higher at lower pH values compared to Ni(II) removal.

The action of forces in the back and abdomen under conditions of loading of different external forces at different bending angles is unexplored area. This paper presents a methodology that enables calculation of the magnitudes of forces in the back and abdominal muscles using the combined techniques of the CATIA software system, appropriate mathematical model and polynomial regression analysis. The person of 180cm in height and 85 kg in weight is loaded with 5 + 5 kg of cargo in both hands, and three cases of bending angles of 150, 300 and 600 relative to the vertical axis are analysed.

In this paper, an analytical calculation of load on bridge crane carts winch wheel loads was performed based on which FEM analysis and topological wheel optimization were performed. After the calculation, a standard wheel diameter was adopted. During FEM analysis in the CAD system, SolidWorks noted that certain surface areas had extremely low stress values, which was the main reason for the topological optimization of the wheel. The topological optimization of the geometric 3D model of the wheel is made in the CAESS ProTOp software, resulting in optimized 3D geometric wheel model. These models offer a number of advantages, such as saving materials to produce, reducing their own weight, balance stress conditions and easy customization model optimized technologies of additive manufacturing. This model of analysis and optimization was performed on the laboratory model of the bridge crane and it is applicable to all types of cranes.

<p style="text-align: justify;">Bearing in mind that the wheel sleeves of motor vehicles are exposed to strong dynamic loads, and knowing that these are very responsible parts of the constructions, the need for permanent researches of the influence of certain variables on the process of production of them is imposed. The prepared part which, in this case, is obtained by forging from the appropriate steel has an important role. The process of forging, implies the appearance of certain errors that can manifest in many ways. Such mistakes, despite rigorous control, are difficult to eliminate completely. The paper deals with prepared parts with errors and their impact on the consistency of the tools, as well as the analysis of the influence of the change in the percentage of some elements on the same.</p>

<p style="text-align: justify;">The fracture of a part or element of a construction can also be defined as a macroscopic separation of the material that leads to the loss of a solid body load. The fracture is the end result of plastic deformation and occurs when all the potential of the material in terms of elastic and plastic deformation is exhausted. This is the worst case of engineering practices, as it can have great consequences for people and the environment.<br />The study of fractures and their causes began with numerous disastrous failures that caused apart from material damage and loss of human lives. These studies have been going on for years, but despite great knowledge, many of the causes of fractures have not been fully established till now. For this reason, research has led to the development of new methods and new testing procedures in conditions similar to exploitation conditions in order to minimize fracture, especially those sudden and unexpected. In this paper, a brief overview of the types of fractures, fracture testing and possible causes of fractures of metal parts during exploitation will be presented.</p>

<p style="text-align: justify;">The main focus of this paper is to present the TQM practices of 593 companies in Bosnia and Herzegovina. In addition, our results confirm that there are statistically significant differences in TQM practices between firms in a highly competitive and less competitive environment, as well as between firms with and without ISO certificates. TQM practices do not differ between companies that belong to different groups that we constructed based on their age, location, export-orientation and the firm size. We contribute to the existing body of knowledge by identifying organizational contextual factors that might matter in designing more complex structural models.<br /><br /></p>

This chapter examines the concept of digital assets from an angle that has not yet been explored in legal scholarship around digital death and the transmission of digital assets on death. Digital death is conceived herein as the death of an individual who leaves behind various digital fragments of their identity, either in the form of digital assets broadly or as digital biographies, dossiers, autobiographies and archives. Digital death causes uncertainty as to what happens in this dispersed, interconnected and often unregulated digital space, which Kasket lucidly entitles The New Elysium. Most legal scholars have considered digital assets either from a perspective of ‘hard law’ of succession and probate or the intersection of property, contracts and intellectual property; sometimes referring to data protection, jurisdiction or cybercrime. The scholars have not ventured into exploring theory that goes beyond theories of property, intellectual property and privacy. The chapter begins by examining classical conceptualisations of digital assets as property and the ‘”new” new property’, exploring whether this is the correct way to perceive digital assets conceptually. It will then go on to examine post-mortem privacy in the context of digital assets, and introduce a novel link with the Floridian concept of informational bodies. In the attempt to offer a comprehensive framework and a more nuanced normative support for future policy and law, the chapter interrelates all of the concepts with the ideas of postmortal society, introducing a new concept of ‘postmortal privacy’. Finally, the author uses this conceptualisation to test some of the existing legal regimes in the area of the transmission of digital assets. Suggestions from this chapter remain mainly at an abstract level, due to the scope and the nature of the framework it introduces.

Human brucellosis during pregnancy is characterized by significantly less pronounced adverse obstetric outcomes than in animals, but with remarkably more adverse obstetric outcomes when compared to healthy pregnant women. Seroprevalence of brucellosis in pregnancy and cumulative incidence of brucellosis cases per 1000 delivered obstetrical discharges in endemic regions were reported to be 1.5–12.2% and 0.42–3.3, respectively. Depending on the region, the frequency of pregnant women in the cohorts of patients with brucellosis was from 1.5% to 16.9%. The most common and the most dramatic unfavorable outcomes during brucellosis in pregnancy are the obstetric ones, manifested as abortions (2.5–54.5%), intrauterine fetal death (0–20.6%), or preterm deliveries (1.2–28.6%), depending on the stage of pregnancy. Other unfavorable outcomes due to brucellosis are addressed to infant (congenital/neonatal brucellosis, low birth weight, development delay, or even death), the clinical course of disease in mother, and delivery team exposure. When diagnosed in pregnant women, brucellosis should be treated as soon as possible. Early administration of adequate therapy significantly reduces the frequency of adverse outcomes. Rifampicin in combination with trimethoprim-sulfamethoxazole for 6 weeks is the most commonly used and recommended regimen, although monotherapies with each of these two drugs are also widely used while waiting for the results from prospective randomized therapeutic trials. As no effective human vaccine exists, screening of pregnant women and education of all women of childbearing age about brucellosis should be compulsory preventive measures in endemic regions.