The paper presents the engineering practice, which the company “Regeneracija” Ltd. Velika Kladuša – Bosnia and Herzegovina uses to perform preliminary experimental testing and measurements, followed by mathematical modeling of critical pressure of these vessels, in order to obtain the projected quality of pressure vessels made of composite materials. The paper will confirm the hypothesis that it is possible to relate mathematical connection and dependence of the critical pressure of vessels of composite materials (Pkr) with mechanical characteristics of vessel material (σM), vessel diameter (D), and vessel wall thickness (s). In this way, by varying the mentioned parameters, it is possible to achieve the desired product quality in the production of composite material containers by achieving the projected critical and thus working pressure. Generally speaking, the mathematical model of critical pressure obtained in this way will be a good indicator for design engineers to know how much critical pressure a given vessel can withstand, and based on that to take quick control of working or projected pressure, but also for designing completely new vessels made of composite materials as a substitute for the expensive experimental testing.

This paper investigates the local and global character of the unique positive equilibrium of a mixed monotone fractional second-order difference equation with quadratic terms. The corresponding associated map of the equation decreases in the first variable, and it can be either decreasing or increasing in the second variable depending on the corresponding parametric values. We use the theory of monotone maps to study global dynamics. For local stability, we use the center manifold theory in the case of the non-hyperbolic equilibrium point. We show that the observed equation exhibits three types of global behavior characterized by the existence of the unique positive equilibrium, which can be locally stable, non-hyperbolic when there also exist infinitely many non-hyperbolic and stable minimal period-two solutions, and a saddle. Numerical simulations are carried out to better illustrate the results.

Natural disasters that cause tremendous material harm and threaten human lives, infrastructure, and economic and social activities become more frequent. While most material and human casualties are the effects of sudden and brief natural disasters, it is not possible to disregard long-term disasters, most frequently triggered by climate change. Damage from long-term disasters is quite significant and hampers the sustainability of the economy and life in densely populated cities. Following climate change, it is concluded that special attention should be paid to coastal cities, cities along rivers and the potential risks of possible disasters should be reduced before they materialize. The reduction of disaster risk is an endeavor involving various professional fields, viewpoints, and stakeholders. An integrated approach that brings together all the necessary elements is therefore required. The planning for protection against natural disasters and in the very moments when it occurs, it is necessary to quickly obtain reliable data to coordinate activities in the field well. However, there are problems in the field with the availability, access, and spatial data use. In this sense, the Spatial Data Infrastructure (SDI) can provide an appropriate framework for sharing spatial data through the cooperation of administration, public, and private institutions and citizens.

Catheter ablation for atrial fibrillation (AF) is one of the most commonly performed electrophysiology procedures. Despite significant advances in our understanding of AF mechanisms in the last years, ablation outcomes remain suboptimal for many patients, particularly those with persistent or long-standing AF. A possible reason is that ablation techniques mainly focus on anatomic, rather than patient-specific functional targets for ablation. The identification of such ablation targets remains challenging. The purpose of this study is to investigate a novel approach based on directed networks, which allow the automatic detection of important arrhythmia mechanisms, that can be convenient for guiding the ablation strategy. The networks are generated by processing unipolar electrograms (EGMs) collected by the catheters positioned at the different regions of the atria. Network vertices represent the locations of the recordings and edges are determined using cross-covariance time-delay estimation method. The algorithm identifies rotational activity, spreading from vertex to vertex creating a cycle. This work is a simulation study and it uses a highly detailed computational 3D model of human atria in which sustained rotor activation of the atria was achieved. Virtual electrodes were placed on the endocardial surface, and EGMs were calculated at each of these electrodes. The propagation of the electric wave fronts in the atrial myocardium during AF is very complex, so in order to properly capture wave propagation patterns, we split EGMs into multiple short time frames. Then, a specific network for each of these time frames was generated, and the cycles repeating in consecutive networks point us to the stable rotor's location. The respective atrial voltage map served as reference. By detecting a cycle between the same 3 nodes in 19 out of 58 networks, where 10 of these networks were in consecutive time frames, a stable rotor was successfully located.

Simple Summary The contamination of feed with mycotoxins is a global concern, resulting in adverse effects on productivity and animal health and, therefore, a great economic loss. Ochratoxin A and T-2 mycotoxins are among the mycotoxins that contaminate animal feed. These mycotoxins could adversely affect the health of broilers, and the most effective method to mitigate the toxic effects of mycotoxins is the use of detoxifying agents. In the present experiment, broiler chickens were allotted into five groups. Group 1 received a non-contaminated diet; group 2 received a non-contaminated diet + 3 g/kg of a mycotoxin binder (MMDA); group 3 received a non-contaminated diet + 0.5 mg/kg OTA + 1 mg/kg T-2 toxin; group 4 received a non-contaminated diet + 0.5 mg/kg OTA + 1 mg/kg T-2 toxin + 1 g/kg MMDA; and group 5 received a non-contaminated diet + 0.5 mg/kg OTA + 1 mg/kg T-2 toxin + 3 g/kg MMDA for 35 days. The results revealed that OTA and T-2 toxin negatively affected the productive parameters and some blood and carcass characteristics of broiler chickens. The addition of the detoxifying agent (MMDA at 1 or 3 g/kg feed) to contaminated diets alleviated the adverse effects observed on productivity and the broilers heath related parameters. Abstract The present study was conducted to evaluate the efficacy of the feed additive, a novel multicomponent mycotoxin detoxifying agent (MMDA) containing modified zeolite (clinoptilolite), Bacillus subtilis, B. licheniformis, Saccharomyces cerevisiae cell walls, and silymarin, as detoxifiers of 0.5 mg/kg (0.5 ppm) ochratoxin A (OTA) and 1 mg/kg (1 ppm) T-2 toxin on broiler chickens. A total of 240 1-old broiler chickens (Ross 308) were randomly distributed into five different dietary treatments: (1) control (non-contaminated diet); (2) non contaminated diet + 3 g/kg of MMDA; (3) non-contaminated diet + 0.5 mg/kg OTA + 1 mg/kg T-2 toxin; (4) non-contaminated diet + 0.5 mg/kg OTA + 1 mg/kg T-2 toxin + 1 g/kg MMDA; and (5) non-contaminated diet + 0.5 mg/kg OTA + 1 g/kg T-2 toxin + 3 g/kg MMDA. The results showed that, in the starter period, from 1 to 10 days, the presence of OTA and T-2 mycotoxins reduced the consumption of feed and the growth of the broilers, and no effects of the detoxifying product were observed in the productivity of the chickens, at any of the doses tested, compared to the contaminated control (treatment 3). However, in the growing period, the same negative effect of mycotoxins was registered, but a recovery was observed in the consumption of feed and in the weight of the broilers that consumed 3 g/kg of the MMDA mycotoxin binder, reaching similar values to those of chickens fed uncontaminated control diets. The presence of mycotoxins in feed led to a reduction in the concentration of total proteins and albumin in blood compared to controls, and the presence of the detoxifying product partially reversed this effect. The breast yield of the chickens fed with mycotoxins was lower than that of the animals fed with the control feed and was not affected by the presence of the product tested, at 1 or 3 g/kg. The weight of the different organs (liver, gizzard, kidneys, or spleen), the intestinal pH, the histology of the small intestine, and oral lesions were not affected by the experimental treatments. In summary, the productive parameters and some blood and carcass characteristics of broiler chickens were impaired by the dietary presence of OTA and T-2 toxin. The tested product included at 1 or 3 g/kg feed in contaminated diets improved performance and seems to be effective in partly counteracting the deleterious effects of the tested mycotoxins.

Aktualna globalna pozicija Bosne i Hercegovine potvrđuje nalaz prethodnih istraživanja da u našoj državi ne postoji jedinstven ili barem harmonizovan i efikasan inovacioni sistem, primjeren potrebama razvoja društva i ekonomije znanja. Prvi koraci u utvrđivanju razloga ovakvog stanja su SWOT analize položaja i uloge inovacionog sistema u okviru opšteg strateškog planiranja i sektorskog planiranja istraživanja i transfera tehnologije. Najvažniji rezultati dobiveni iz tih matrica su diskutovani u radu. Oni su potom uspoređeni sa rezultatima naših najnovijih istraživanja. Prvi rezultat je sažeta SWOT matrica inovacionog sistema. Rezultate razrade pojedinih faktora smo prikazali u detaljnoj SWOT matrici zasnovanoj na elementima snage. I ova matrica je pokazala da je ujednačeno i stabilizirano visoko obrazovanje najvažniji element snage inovacionog sistema. Zbog toga su obilježja univerziteta kao subjekta inovacionog sistema posebno razmotrena.

UDK: 630*9:502.1(234.422 Vranica) Nature conservation and sustainable management of forest resources become more important in Bosnia and Herzegovina, driven by the accession process toward the European Union as well as other international processes directed toward responsible management of forest resources. The forest certification has been widely adopted in the forestry sector and it implies meeting the sustainable forest management standard, whereas identification and proper management of high conservation value forests are one of the basic requirements. The NATURA 2000 ecological network is to become an important driver of reforms in the field of nature protection and forestry sector, due to the designation of new sites in forest area, which are under the responsibility of forestry institutions. This paper illustrates the scientific understanding of identification and management processes related to high conservation value forests that were proposed within potential NATURA 2000 habitats. The paper is based on analysis of main guiding principles for site designation and the role of the cross-sectoral approach applied identification and management of sites with high conservation value attributes. The case study research design was selected focusing on the Vranica Mountain due to recent activities implemented in this site. The in-depth face-to-face interviewing was used to collect qualitative data containing the key stakeholders' attitudes regarding the harmonization of NATURA 2000 habitats with the high conservation value forests – (HCVF), as well as the involvement of stakeholders in the processes of cross-sectoral cooperation. Results of this paper can be useful for the key forest and nature protection policy-makers, as well as to those responsible for managing of protected areas, or other stakeholders directly or indirectly involved in the process of identification and management of HCVFs and NATURA 2000 sites. Harmonisation of guiding principles and cross-sectoral cooperation during the identification and management of HCVFs and NATURA 2000 forest habitats enables the proper implementation of conservation and management measures based on sustainable forest management activities.

Ex-vivo expanded tumour infiltrating lymphocytes (TIL) show promise in delivering durable responses among several solid tumour indications. However, characterising, quantifying and tracking the active component of TIL therapy remains challenging as the expansion process does not distinguish between tumour reactive and bystander T-cells. Achilles Therapeutics has developed ATL001, a patient-specific TIL-based product, manufactured using the VELOS™ process that specifically targets clonal neoantigens present in all tumour cells within a patient. Two Phase I/IIa clinical trials of ATL001 are ongoing in patients with advanced Non-Small Cell Lung Cancer, CHIRON (NCT04032847), and metastatic or recurrent melanoma, THETIS (NCT03997474). Extensive product characterisation and immune-monitoring are performed through Achilles’ manufacturing and translational science programme. This enables precise quantification and characterisation of the active component of this therapy – Clonal Neoantigen T cells (cNeT) – during manufacture and following patient administration, offering unique insight into the mechanism of action of ATL001 and aiding the development of next generation processes.ATL001 was manufactured using procured tumour and matched whole blood from 8 patients enrolled in the THETIS (n=5) and CHIRON (n=3) clinical trials. Following administration of ATL001, peripheral blood samples were collected up to week 6. The active component of the product was detected via re-stimulation with clonal neoantigen peptide pools and evaluation of IFN-γ and/or TNF-α production. Deconvolution of individual reactivities was achieved via ELISPOT assays. Immune reconstitution was evaluated by flow cytometry. cNeT expansion was evaluated by restimulation of isolated PBMCs with peptide pools and individual peptide reactivities (ELISPOT).The median age was 57 (range 30 – 71) and 6/8 patients were male. The median number of previous lines of systemic anti-cancer treatment at the time of ATL001 dosing was 2.5 (range 1 – 5). Proportion of cNeT in manufactured products ranged from 0.20% - 77.43% (mean 26.78%) and unique single peptide reactivities were observed in 7 of 8 products (range 0 – 28, mean 8.6). Post-dosing, cNeTs were detected in 5/8 patients and cNeT expansion was observed in 3/5 patients.These data underscore our ability to sensitively detect, quantify and track the patient-specific cNeT component of ATL001 – during manufacture and post dosing. As the dataset matures, these metrics of detection and expansion will be correlated with product, clinical and genomic characteristics to determine variables associated with peripheral cNeT dynamics and clinical response.NCT04032847, NCT03997474The first 8 patients described have all been located within the UK and both trials (CHIRON and THETIS) have been approved by the UK MHRA (among other international bodies, e.g FDA). Additionally, these trials have been approved by local ethics boards at active sites within the UK. Patient‘s are fully informed by provided materials and investigators prior to consenting to enrol into either ATL001 trial.

One of the advantages provided by fused deposition modelling (FDM) 3D printing technology is the manufacturing of product materials with infill structure, which provides advantages such as reduced production time, product weight and even the final price. In this paper, the tensile mechanical properties, tensile strength and elastic modulus, of PLA, Tough PLA and PC FDM 3D printed materials with the infill structure were analysed and compared. Also, the influence of infill pattern on tensile properties was analysed. Material testing were performed according to ISO 527-2 standard. All results are statistically analysed and results showed that infill pattern have influence on tensile mechanical properties for all three materials.