The low seroprevalent human adenovirus type 26 (HAdV26)-based vaccine vector was the first adenovirus-based vector to receive marketing authorization from European Commission. HAdV26-based vaccine vectors induce durable humoral and cellular immune responses and, as such, represent a highly valuable tool for fighting infectious diseases. Despite well-described immunogenicity in vivo, the basic biology of HAdV26 still needs some refinement. The aim of this study was to determine the pro-inflammatory cytokine profile of epithelial cells infected with HAdV26 and then investigate the underlying molecular mechanism. The expression of studied genes and proteins was assessed by quantitative polymerase chain reaction, western blot, and enzyme-linked immunosorbent assay. Confocal microscopy was used to visualize HAdV26 cell uptake. We found that HAdV26 infection in human epithelial cells triggers the expression of pro-inflammatory cytokines and chemokines, namely IL-6, IL-8, IL-1β, and TNF-α, with the most pronounced difference shown for IL-6. We investigated the underlying molecular mechanism and observed that HAdV26-induced IL-6 gene expression is αvβ3 integrin dependent and NF-κB mediated. Our findings provide new data regarding pro-inflammatory cytokine and chemokine expression in HAdV26-infected epithelial cells, as well as details concerning HAdV26-induced host signaling pathways. Information obtained within this research increases our current knowledge of HAdV26 basic biology and, as such, can contribute to further development of HAdV26-based vaccine vectors.

Ayurvedic medicines are categorized into seven constitutional forms ‘Prakriti’ which is a constituent in the Ayurvedic system of medicine to determine drought tolerance and drug responsiveness. Prakriti assessment entails a thorough physical examination as well as queries about physiological or behavioral characteristics. The prevalence of certain "doshas" is attributed by Ayurveda to the fundamental constituent of a person. Vata, pitta, and Kapha are the three main doshas mentioned. Ayurveda-dosha studies have been used for a long time, but the quantitative reliability measurement of these diagnostic methods still lags. The careful and appropriate analysis leads to an effective treatment. In this paper, we demonstrate the result of certain machine learning methods like Support Vector Machine (SVM), Naive Bayes (NB), Decision Tree (DT), K-Nearest Neighbour (KNN), Artificial Neural Network (ANN), and Adaboost algorithm for various performance characteristics to predict human body constituencies. From the observations of results it is shown that the AdaBoost algorithm with hyperparameter tuning provides enhanced accuracy and recall of 0.97, precision and F-score of 0.96, the lower RSME value obtained is 0.64. The experimental results reveal that the improved model, which is based on ensemble learning methods, outperforms traditional methods significantly. According to the findings, advancements in the proposed algorithms could give machine learning a promising future.

The use of the biological medicines, also called “biologics,” has contributed to the progress of the treatment of many chronic diseases, such as cancer, rheumatoid arthritis, Crohn’s disease, multiple sclerosis, and psoriasis. However, biologicals are expensive for healthcare systems in several countries. Their availability has been a global issue, which has affected many patients that suffer from various diseases. A biosimilar medicine, also called “biosimilar,” is a medicine with similar characteristics in terms of quality, biological activity, safety, and efficacy as the approved original biological medicine, known as “originator biologic.” Biosimilars generate competition within the market because they lower the prices of biologics and thus allow for an increase in patient access. However, there are barriers when it comes to the acceptability rate of biosimilars and how interchangeable they are with the originator biologic. In this review, we present a national regulatory framework for biologics along with its limitations, a system of monitoring the safety profile of biologics, the guideline for interchangeability, and a list of approved and available biologics in Bosnia and Herzegovina. Additionally, recommendations were made here in order to provide opportunities for greater acceptance of biosimilars and better access to biologics. These recommendations include, but are not limited to, strengthening the national regulatory framework for biologics, capacity building, increasing awareness among healthcare providers for reporting adverse drug events and active pharmacovigilance, and better definitions of interchangeability. Finally, awareness among healthcare providers regarding biosimilars and biologics should be raised through continuous education and workshops, and by including this important topic in the graduate and postgraduate curriculum programs in the country.

Aims: Industrial processed food, pharmaceutical and cosmetical products contain numerous substances the safety of which has been widely concerned. Potassium sorbate (PS) is a common preservative used in a wide-range of products. Sunset yellow (SY) is one of the most commonly used dye in food and pharmaceutical industry. Several studies suggested cytotoxic and genotoxic potential of SY and PS in different cell lines. These effects can lead to organ damage and cancer development. The aim of this study is to investigate cytotoxic and genotoxic potential of two widely used food additives, SY and PS, in Jurkat cell line. Methodology: PrestoBlue assay was used to assess the cytotoxic potential of SY and PS. For observation of DNA damage, cytostatic and cytotoxic effects, the cytokinesis-block micronucleus cytome (CBMN) assay was performed. Results: Decrease of cell viability in Jurkat cell line was observed after 24-hour exposure to both SY and PS. CBMN assay has revealed significant increase of necrotic cells (P<0.05). Genotoxic biomarkers were in physiological range after 24-hour exposure to both analyzed additives. Conclusion: Our findings suggest that SY, as well as PS have cytotoxic potential in Jurkat cell line, as a result of increased number of necrotic cells. Higher cytotoxic effect was caused by SY compared to PS. However, genotoxic potential was not recorded for any of the food additives analyzed.

Cardiovascular diseases (CVDs) are the leading cause of morbidity and mortality in the adult population worldwide and represent a severe economic burden and public health concern. The majority of human genes do not code for proteins. However, noncoding transcripts play important roles in ageing that significantly increases the risk for CVDs. Noncoding RNAs (ncRNAs) are critical regulators of multiple biological processes related to ageing such as oxidative stress, mitochondrial dysfunction and chronic inflammation. NcRNAs are also involved in pathophysiological developments within the cardiovascular system including arrhythmias, cardiac hypertrophy, fibrosis, myocardial infarction and heart failure. In this review article, we cover the roles of ncRNAs in cardiovascular ageing and disease as well as their potential therapeutic applications in CVDs.

Gingiva-Derived Mesenchymal Stromal Cells (GMSCs) have been shown to play an important role in periodontitis. However, how P. gingivalis, one of the key etiological agents of the disease, affects healthy (H)- and periodontitis (P)-GMSCs is unknown. To address this problem, we established 10 H-GMSC and 12 P-GMSC lines. No significant differences in morphology, differentiation into chondroblasts and adipocytes, expression of characteristic MSCS markers, including pericyte antigens NG2 and PDGFR, were observed between H- and P-GMSC lines. However, proliferation, cell size and osteogenic potential were higher in P-GMSCs, in contrast to their lower ability to suppress mononuclear cell proliferation. P. gingivalis up-regulated the mRNA expression of IL-6, IL-8, MCP-1, GRO-α, RANTES, TLR-2, HIF-1α, OPG, MMP-3, SDF-1, HGF and IP-10 in P-GMSCs, whereas only IL-6, MCP-1 and GRO-α were up-regulated in H-GMSCs. The expression of MCP-1, RANTES, IP-10 and HGF was significantly higher in P-GMSCs compared to H-GMSCs, but IDO1 was lower. No significant changes in the expression of TLR-3, TLR-4, TGF-β, LAP, IGFBP4 and TIMP-1 were observed in both types of GMSCs. In conclusion, our results suggest that P-GMSCs retain their pro-inflammatory properties in culture, exhibit lower immunosuppressive potential than their healthy counterparts, and impaired regeneration-associated gene induction in culture. All these functions are potentiated significantly by P. gingivalis treatment.

In this paper we explore two types of tilings of a honeycomb strip and derive some closed form formulas for the number of tilings. Furthermore, we obtain some new identities involving tribonacci numbers, Padovan numbers and Narayana's cow sequence and provide combinatorial proofs for several known identities about those numbers.

A macroscopic theory of high-order harmonic generation (HHG) is presented, which applies a focal-averaging method based on the integral solution of the wave equation. The macroscopic high-harmonic yield is the coherent superposition of the single-atom contributions of all atoms of the generating medium, which are positioned at different spatial points of the laser focus and exposed to the space-time-dependent laser pulse. The HHG spectrum obtained in our macroscopic simulations is qualitatively different from the one obtained using the microscopic or single-atom theory of HHG. Coherent intensity focal averaging, the simpler and more approximate of two methods we introduced, gives the spectrum which forms a declining plateau with the same cutoff position as that of the microscopic spectrum. The second, more precise method, which we call coherent spatio-temporal focal averaging, shows that it is possible, changing the macroscopic conditions, to obtain an observable peak in the harmonic spectrum at an energy much lower than the microscopic cutoff energy. Generally, the high-harmonic yield appears to be dominated by the contributions of laser-pulse spatio-temporal regions with lower intensities as well as by interference, so that the high-energy plateau and its sharp cutoff are quenched in the theoretical simulation and, presumably, in the experiment. The height and position of this peak strongly depend on the macroscopic conditions. We confirmed these findings by applying our macroscopic theory to simulate two recent experiments with mid-infrared laser fields, one with a linearly polarized field and the other one with a bicircular field.

The paper deals with theoretical and methodological aspects of examining the position of religion within the university context. The first section offers an overview of assumptions within three recent pieces of research on this topic. In its second section, the paper examines the possibility of applying the curriculum theory to investigating the position of religion within the higher education context of public universities in Bosnia and Herzegovina. The results show that, in the analysed programmes, there is still no separate subject that would, as an introductory course, familiarise students with doctrinal and ritual fundamentals of religions, as well as with the main events of their historical existence. At the same time, there are a certain number of courses that include religion into a wider discourse of a given study, treating it as a philosophical, sociological, historical or cultural phenomenon. The paper also identifies courses that, within a wider theoretical discussion, include the issue of religion, whether as a substantive or an accidental element.

Tamim Ansary, Ometena sudbina: Historija svijeta u očima muslimana, s engleskog prevela Azra Mulović, Sarajevo: Centar za napredne studije, 2020. Str. 472. ISBN 978-9926-471-22-4.

Howard Ball, Working in the Killing Fields: Forensic Science in Bosnia, Potomac Books, 232 str, 2016, ISBN 978-1-61234-730-1

In the last two decades the question of displaying religious symbols in the public sphere has resulted in more intensified discussions within the debates on freedom of faith, belief and the neutrality of state. Strategies of resolution of this question greatly depend on the adopted model of relationship between state and religion. While the USA model of freedom of religion regulates extensively the question of the presence of religious symbols in the public sphere, the French model of laïcité is characterised by the general legal ban on displaying them in public institutions since 1905. Some states permit displaying symbols of the dominant religion in the public sphere, while at the same time prohibiting or limiting presence of symbols of the minority religions.

In the last 5 years, several projects have been initiated in Sarajevo Canton that are focused on strengthening the identity of the city, promoting and implementing the concept of urban mobility, green and smart city. The issue of energy efficiency is included in all projects and from that aspect, among other things, the improvement of the public city passenger transport service is observed. This paper analyzes the state of public urban transport from the energy aspect, the savings that are achieved, the methodology for calculating savings and the effects that would be achieved through the implementation of planned projects for the development and reorganization of public urban passenger transport.