Significant efforts have been done in last two decades to develop nanoscale spectroscopy techniques owning to their great potential for single-molecule structural detection and in addition, to resolve open questions in heterogeneous biological systems, such as protein–DNA complexes. Applying IR-AFM technique has become a powerful leverage for obtaining simultaneous absorption spectra with a nanoscale spatial resolution for studied proteins, however the AFM-IR investigation of DNA molecules on surface, as a benchmark for a nucleoprotein complexes nanocharacterization, has remained elusive. Herein, we demonstrate methodological approach for acquisition of AFM-IR mapping modalities with corresponding absorption spectra based on two different DNA deposition protocols on spermidine and Ni2+ pretreated mica surface. The nanoscale IR absorbance of distinctly formed DNA morphologies on mica are demonstrated through series of AFM-IR absorption maps with corresponding IR spectrum. Our results thus demonstrate the sensitivity of AFM-IR nanospectroscopy for a nucleic acid research with an open potential to be employed in further investigation of nucleoprotein complexes.

Simple Summary With 30% of clinically negative early-stage oral cancer patients harboring occult metastasis, an accurate staging of metastatic lymph nodes (LN) is of utmost importance for treatment planning. A magnetic sentinel lymph node biopsy (SLNB) procedure is offered as an alternative to conventional SLNB in oral oncology, however, a grading system is missing. A proper grading system is preferred to connect the different components of the magnetic SLNB: preoperative imaging, intraoperative detection, and histopathological examination of sentinel lymph nodes (SLNs). This study aims to provide a first grading system based on the distribution of a magnetic tracer, by means of preoperative magnetic resonance imaging (MRI), intraoperative estimation of iron content, and histopathological assessment of resected nodes. Pre- and post-operative MRI and harvested SLNs of eight tongue cancer patients with successful magnetic SLNB procedure were used for analyses. Abstract A magnetic sentinel lymph node biopsy ((SLN)B) procedure has recently been shown feasible in oral cancer patients. However, a grading system is absent for proper identification and classification, and thus for clinical reporting. Based on data from eight complete magnetic SLNB procedures, we propose a provisional grading system. This grading system includes: (1) a qualitative five-point grading scale for MRI evaluation to describe iron uptake by LNs; (2) an ex vivo count of resected SLN with a magnetic probe to quantify iron amount; and (3) a qualitative five-point grading scale for histopathologic examination of excised magnetic SLNs. Most SLNs with iron uptake were identified and detected in level II. In this level, most variance in grading was seen for MRI and histopathology; MRI and medullar sinus were especially highly graded, and cortical sinus was mainly low graded. On average 82 ± 58 µg iron accumulated in harvested SLNs, and there were no significant differences in injected tracer dose (22.4 mg or 11.2 mg iron). In conclusion, a first step was taken in defining a comprehensive grading system to gain more insight into the lymphatic draining system during a magnetic SLNB procedure.

The aim of the study was to assess the seroprevalence of SARS-CoV-2 in the Republic of Srpska, Bosnia and Herzegovina, and to analyse the knowledge, attitudes and practices of the population toward COVID-19. This population-based study was conducted in a group of 1,855 randomly selected individuals from all municipalities from 1 December 2020 to 15 January 2021. All individuals were asked to sign a consent form and to fill in a questionnaire, following which a blood samples were collected. Total anti-SARS-CoV-2 antibodies were determined in serum specimens using the total Ab ELISA assay. The overall seroprevalence rate was 40.3%. Subjects aged <65 years were 2.06 times more likely to be seropositive than those aged ≥65, and 30% of seropositive individuals presented no COVID-19 symptoms. The household members of seropositive individuals were 2.24 times more likely to develop COVID-19 symptoms than the household members of seronegative individuals. More than 95% of respondents believe that preventive measures are very important to control the infection transmission. Majority of respondents wear the masks properly, maintain the required physical distance whenever possible and wash hands with soap. Nearly 50% of individuals were of the opinion that the vaccine could prevent the infection. This study showed that an overall SARS-CoV-2 seropositivity rate by the middle of January 2021 was very high. Attitudes and practices regarding the COVID-19 indicate that additional efforts should be taken in order to improve the health education with a focus on preventive measures and vaccination.

Cycling is one of the most demanding sports in terms of aerobic ability. Individual profiling, selection of cyclists implies adequate analysis of anthropometric characteristics and body composition. In addition to the analysis of anthropometric characteristics and body composition, it is necessary to detect and assess motor (physical) abilities, which are often defined by the term fitness profile. In this study, the anthropometric space, body composition and fitness abilities of cyclist M.M, a member of the Cycling Club "Borac" from Čačak and a member of the Serbian national team were analyzed in detail. As many as 60 variants were measured to assess the defined segments (anthropometric space, body composition, fitness profile). The obtained results of anthropometry and physical status confirmed the presence of the ectomorphic-mesomorphic somatotype of the cyclist, which is represented in the so-called road disciplines and endurance disciplines. Also, the fitness profile of the competitor determines extremely good results in all motor skills. To conclude that in addition to the dominance of aerobic abilities, cyclists define exceptional parameters of anaerobic abilities and fitness (motor) abilities (strength, speed, coordination, ...) and their pronounced synergistic effect.  Article visualizations:

A growing body of work has highlighted the importance of political beliefs and attitudes in predicting endorsement and engagement in prosocial behavior. Individuals with right-wing political orientation are less likely to behave prosocially than their left-wing counterparts due to high levels of Right-wing authoritarianism (RWA). Here, we aimed to extend prior work by testing how political values relate to COVID-19 discretionary behavioral intentions (i.e., prosocial and non-mandatory behaviors aimed at controlling the spread of the pandemic). Furthermore, we tested whether identification with the national group would influence the relationship between RWA and prosocial behavior. A cross-sectional study conducted on 350 Italian participants showed that right-wing political orientation had a negative effect on COVID-19 discretionary behavioral intentions via RWA. Furthermore, a moderated mediation model revealed that this effect was only significant for participants who are lowly identified with the national group. The results suggest that highlighting group belongingness might effectively motivate more conservative individuals to engage in prosocial behavior.

The conductivity and the state of the surface of supports are of vital importance for metallization via electrodeposition. In this study, we show that the metallization of a carbon fiber-reinforced polymer (CFRP) can be carried out directly if the intermediate graphene oxide (GO) layer is chemically reduced on the CFRP surface. Notably, this approach utilizing only the chemically reduced GO as a conductive support allows us to obtain insights into the interaction of rGO and the electrodeposited metal. Our study reveals that under the same contact current experimental conditions, the electrodeposition of Cu and Ni on rGO follows significantly different deposition modes, resulting in the formation of three-dimensional (3D) and free-standing metallic foils, respectively. Considering that Ni adsorption energy is larger than Ni cohesive energy, it is expected that the adhesion of Ni on rGO@CFRP is enhanced compared to Cu. In contrast, the adhesion of deposited Ni is reduced, suggesting diffusion of H+ between rGO and CFRP, which promotes the hydrogen evolution reaction (HER) and results in the formation of free-standing Ni foils. We ascribe this phenomenon to the unique properties of rGO and the nature of Cu and Ni deposition from electrolytic baths. In the latter, the high adsorption energy of Ni on defective rGO along with HER is the key factor for the formation of the porous layer and free-standing foils.

During the last few decades, great research endeavors have been applied to healthcare robots, aiming to develop companions that extend the independent living of elderly people. To deploy such robots into the market, it is expected that certain applications should be addressed with repeatability and robustness. Such application is the assistance with medication-related activity, a common need for the majority of elderly people, referred from here on as medication adherence. This paper presents a novel and complete pipeline for assistance provision in monitoring and serving of medication, using a mobile manipulator embedded with action, perception and cognition skills. The challenges tackled in this work comprise, among others, that the robot locates the medication box placed in challenging spots by applying vision based strategies, thus enabling robust grasping. The grasping is performed with strategies that allow environmental contact, accommodated by the manipulator’s admittance controller which offers compliance behavior during interaction with the environment. Robot navigation is applied for the medication delivery, which, combined with active vision methods, enables the automatic selection of parking positions, allowing efficient interaction and monitoring of medication intake activity. The robot skills are orchestrated by a partially observable Markov decision process mechanism which is coupled with a task planner. This enables assistance scenario guidance and offers repeatability as well as gentle degradation of the system upon a failure, thus avoiding uncomfortable situations during human–robot interaction. Experiments have been conducted on the full pipeline, including robot’s deployment in 12 real house environments with real participants that led to very promising results with valuable findings for similar future applications.

A conciliação medicamentosa tem como objetivo prevenir erros de medicação resultantes de discrepâncias da prescrição, e associada à anamnese, permite conhecer de forma global a história de saúde do paciente. O início da pandemia pelo novo coronavírus, causador da Covid-19, impôs o isolamento social, circunstância que afetou diretamente o acesso ao serviço de conciliação medicamentosa. Para realizá-la, o farmacêutico precisa entrevistar o paciente ou seus acompanhantes, especialmente quando este possui dificuldades de comunicação e entendimento, ou está em uso de intubação orotraqueal (IOT). O objetivo foi implementar um serviço de conciliação medicamentosa via remota, para obter dados destinados ao desenvolvimento adequado da farmacoterapia,além de garantir a assistência completa, visando a segurança do paciente desde a admissão hospitalar. Após a implementação do serviço remoto, em março de 2021, foi verificada a ampliação do número de pacientes comconciliação medicamentosa realizada nas Unidades de Terapia Intensiva de Covid-19, chegando a uma taxa média de 99,2%. O índice garantiu uma melhoria na linha de cuidado do paciente durante a internação hospitalar.

Problem-based learning (PBL) allows students to learn medical statistics through problem solving experience. The aim of this study was to assess the efficiency of PBL modules implemented in the blended learning courses in medical statistics through knowledge outcomes and student satisfaction. The pilot study was designed as a randomized controlled trial that included 53 medical students who had completed all course activities. The students were randomized in two groups: the group with access to PBL modules within the blended learning course (hPBL group) and the group without access to PBL modules–only blended learning course (BL group). There were no significant differences between the groups concerning socio-demographic characteristics, previous academic success and modality of access to course materials. Students from hPBL group had a significantly higher problem solving score (p = 0.012; effect size 0.69) and the total medical statistics score (p = 0,046; effect size 0.57). Multivariate regression analysis with problem solving as an outcome variable showed that problem solving was associated with being in hPBL group (p = 0.010) and having higher grade point average (p = 0.037). Multivariate regression analysis with the medical statistics score as an outcome variable showed the association between a higher score on medical statistics with access to PBL modules (p = 0.045) and a higher grade point average (p = 0.021). All students in hPBL group (100.0%) considered PBL modules useful for learning medical statistics. PBL modules can be easily implemented in the existing courses within medical statistics using the Moodle platform, they have high applicability and can complement, but not replace other forms of teaching. These modules were shown to be efficient in learning, to be well accepted among students and to be a potential missing link between teaching and learning medical statistics. The authors of this study are planning to create PBL modules for advanced courses in medical statistics and to conduct this study on other universities with a more representative study sample, with the aim to overcome the limitations of the existing study and confirm its results.

O objetivo deste estudo foi identificar o perfil clínico-funcional de idosas residentes de uma instituição de longa permanência por meio do instrumento Índice de Vulnerabilidade Clínico Funcional-20 (IVCF-20). Compuseram a amostra 29 idosas, com idade a partir de 65 anos. Tal instrumento é composto por 20 questões que contemplam aspectos multidimensionais da condição de saúde do idoso e o classifica em robusto, com risco de fragilização ou frágil. Os resultados obtidos no presente estudo categorizaram majoritariamente idosas com risco de fragilização.

OBJECTIVE Cystatin C (Cys-C) concentration has not been examined sufficiently among healthy newborn population, particularly in terms of reference values. This study aimed to establish gender-, postnatal age- and birth weight-specific Cys-C concentration for healthy term newborns. Its objective was also to examine if there were any differences between our measured concentration and the reference interval established by the CALIPER study. METHODS Serum samples from a total of 90 healthy term newborns were used to determine Cys-C concentration. Cys-C was measured within first three days of birth using particle-enhanced turbidimetric immunoassay (PETIA) on the Architect plus ci8200 analyzer. RESULTS Median concentration of the Cys-C was 2.05 mg/L. There were no statistically significant differences in Cys-C concentration regarding gender (p=0.779), birth weight (p=0.505), postnatal age (p=0.512) or Apgar score (p=0.799). The value of the 2.5th and 97.5th percentile for Cys-C concentrations for girls was 0.93-3.15 mg/L and for boys it was 1.5-3.36 mg/L. CONCLUSION Cys-C concentration in healthy term newborns does not depend on gender, birth weight, postnatal age, or Apgar score. Our measured concentration range of CyS-C in healthy newborns turned out to be slightly wider than the interval determined in the CALIPER study.

(JMIR Cardio 2022;6