UDK 582.475:577.151.64(497.6) Uporabom 13 izoenzimskih sustava, te analizom 20 gen lokusa, analizirali smo genetičku strukturu populacije 13 populacija smreke (Picea abies (L.) Karst.) iz Bosne i Hercegovine. Rezultati izoenzimske analize su vrijednovani preko alelnih frekvencija koje su obračunate iz razlika između gen lokusa, efektivnog broja alela i te stvarne i očekivane heterozigotnosti. Genetička struktura je prikazana za sve gen lokuse koji su pokazali polimorfizam, u istraživanim populacijama smreke.

UDK 630*44:582.632.2          582.632.2:582.728.4 Hrast kitnjak (Quercus petraea Liebl.) u šumama Bosne i Hercegovine već duži niz godina je izložen brojnim negativnim uticajima. Poseban značaj u procesu odumiranja stabala hrasta kitnjaka ima hrastova imela - Loranthus europaeus Jacq. U ovom radu analizirano je prisustvo parazitske cvjetnice na stablima hrasta. Rezultati pokazuju značajnu pojavu imele u objektu istraživanja kao bitnog faktora destabilizacije šumskih sastojina. U odmakloj fazi razvoja poluparazita redovito dolazi do pojave ulančavanja drugih štetnih biotičkih i abiotičkih agenasa.

Seismotectonics of Bosnia seems to follow the Mediterranean marine regime. Earthquakes occur mostly in the outer Dinaric Alps (southern Bosnia), while the strongest earthquakes occur within the Sarajevo Fault system in southern and northwestern Bosnia. In addition to active tectonics being strong, crustal earthquakes occur often as well. Due to rich hydrogeology, crustal loading such as by snow and rain, or reservoir inundation, represents the most important secondary seismogenic source in the region. Despite its exquisite and active geomorphology no comprehensive and reliable geodynamical studies exist on the region. Seismic sensors coverage is extremely poor also. One centenary analogue, and a few recently installed digital seismometers are insufficient for a region that exhibits mild to high seismic activity. Significant investments are needed in order for GPS, seismic and other sensor instrumented networks to be put in place or enhanced. Technical personnel needs to be educated to enable support provide for studies that are done within broader scientific activities. Such efforts that presently seek to include Bosnia under their scope are ESF COST Action 625, NATO Stability Pact DPPI program, and EUREF CERGOP geophysics projects.

Pineal gland has and antistressogenic role. Its main hormone, melatonin, has radio protective effect on endocrine and other dynamic tissues. In our previous study, we have shown that pinealectomy changes the behavior of suprarenal gland in totally irradiated rats. The aim of this study is to evaluate the effect of exogenous melatonin on suprarenal gland of rats with or without pineal gland. Four months after pinealectomy (experimental group) or shampinealectomy (control group), adult Wistar male rats were daily treated with 0,2 mg of melatonin intraperitoneally, during two weeks. Thereafter, all animals were totally irradiated with 8 Gy of Gamma rays produced from Cobalt 60. Animals who survived were sacrificed on the 17(th) post irradiation day. Qualitative and quantitative characteristics of the suprarenal gland were studied using histological methods. The results show that exogenous melatonin had protective role on suprarenal gland in totally irradiated rats and that those effects were more pronounced in the presence of pineal gland.

The coronary collateral circulation is an alternative source of blood supply to the myocardium jeopardized by the failure of the original stenotic or occluded vessel to provide adequate blood flow to this region. One hundred coronary angiograms and left ventriculograms of patients with coronary artery disease from the Cardiology Department of University Clinics Centre in Sarajevo were reviewed. The role of collateral circulation in preserving myocardial function was assessed by comparing regional left ventricular contractility in 34 instances of total arterial occlusion and adequate colateral circulation with that in 34 instances of total arterial occlusion and inadequate collateral circulation. Among the group with adequate collaterals, regional left ventricular contraction was normal in 41%, hypokinetic in 53% and akinetic or dyskinetic in only 5%. Among the group with inadequate collaterals, regional contraction was normal in 9%, hypokinetic in 20 % and akinetic or dyskinetic in 70%. These data indicate that collateral circulation plays an important role in preserving myocardial contractility in patients with coronary artery disease.

The aim of our study was to establish the influence of antidepressive drugs on serum and brain beta-endorphins in experimental animals. Experiment was performed on albino Wistar rats. Antidepressant amitryptiline was used, and for quantification of sera and brain beta-endorphins RIA technique. Our results showed difference between sera and brain beta-endorphins concentration in amitryptiline pretreated animals, vs. those in serum and brain of control group treated with 0.95% NaCl. This study shows that use of psychoactive drugs have influence on sera and brain beta-endorphins concentration. Beta-endorphins could be of great importance, used as markers for evaluation of antidepressant drug effects.

During the 1992-1995 siege, as well as after the war activities, citizens of Sarajevo were most probably exposed to various potential genotoxic agents. The effects of those potential genotoxins were evaluated by micronucleus-cytokinesis blocked assay. The study included 30 individuals who resided in the area of Sarajevo during the war and the postwar period. Point bi-serial coefficient analysis did not reveal any relationship between the frequencies of binuclear cells with micronuclei as well as total number of micronuclei and smoking habits or gender. Simple linear regression revealed statistically significant positive correlation between the age and micronuclei formation. Due to the war related environmental contamination more extensive study is recommended.

Following the abdominal surgery, extensive adhesions often occur and they can cause difficulties during laparoscopic operations. However, previous laparotomy is not considered to be a contraindication for laparoscopy. The aim of this study is to present that an insertion of Veres needle in the region of umbilicus is a safe method for creating a pneumoperitoneum for laparoscopic operations after previous laparotomy. In the last three years, we have performed 144 laparoscopic operations in patients that previously underwent one or two laparotomies. Pathology of digestive system, genital organs, Cesarean Section or abdominal war injuries were the most common causes of previous laparotomy. During those operations or during entering into abdominal cavity we have not experienced any complications, while in 7 patients we performed conversion to laparotomy following the diagnostic laparoscopy. In all patients an insertion of Veres needle and trocar insertion in the umbilical region was performed, namely a technique of closed laparoscopy. Not even in one patient adhesions in the region of umbilicus were found, and no abdominal organs were injured.