Takotsubo syndrome (TTS) is a stress-induced cardiomyopathy, characterized by an increased concentration of catecholamines, free radicals, and inflammatory cytokines, endothelial dysfunction, and increased apoptotic activity. High doses of isoprenaline are used in animal models to induce Takotsubo (TT)-like myocardial injury. The aim of the study was to investigate the antiapoptotic effects of liraglutide in experimental TTS and its role in the NF-κB pathway. Wistar rats were pretreated with liraglutide for 10 days, and on days 9 and 10, TT-like myocardial injury was induced with isoprenaline. After the sacrifice on day 11, hearts were isolated for histopathological and immunohistochemical analysis. Liraglutide reduced isoprenaline-induced cardiomyocyte apoptosis by decreasing cleaved caspase-3 (CC3), BCL-2-associated X protein (BAX), and NF-κB and increasing B-cell lymphoma/leukemia-2 (BCL-2). An increase in NF-κB in isoprenaline-treated rats was in positive correlation with proapoptotic markers (BAX and CC3) and in negative correlation with antiapoptotic marker BCL-2. Liraglutide increased BCL-2 and decreased NF-κB, BAX, and CC3, preserving the same correlations of NF-κB to apoptotic markers. It is concluded that liraglutide protects cardiomyocytes against isoprenaline-induced apoptosis in experimental TT-like myocardial injury through downregulation of the NF-κB pathway.

Aim To investigate an association between eye injury and later academic and social performance. Materials A retrospective longitudinal study of taking measurements multiple times was conducted including all severe eye injuries admitted during 2011-2017 at the main regional clinic to investigate changes in academic success and social inclusion before and after an injury. Parents/caregivers were surveyed asking questions on academic performance, and community/social involvement. In 2023 data on employment status were obtained. Kappa (ĸ) and non-parametric Wilcox-on signed-rank test for multiple comparisons were used. For association of employment status and post-injury visual acuity Fisher score was tested. Results The total sample for assessing social inclusion and academic success was 36 and 25 children, respectively. In the following academic year ĸ agreements decreased from 0.88 (p<0.001) comparing pre-injury with a one-year post to 0.5106 (p<.0001) in the second year and 0.4750 (p=0.003) in the third, suggesting a deteriorating trend. A comparison of academic success before injury to two and three years after injury showed the trend significantly worsening with time (p=0.005, and 0.003, respectively). No association between an eye injury and social inclusion, as well as between employment and final visual acuity was noticed. Conclusion Our findings suggest no association between social inclusion, employment, and eye trauma, while in later years academic success may be more impacted by the eye injury. Due to eye injury unpredictability in childhood age, both an informed and supportive climate environment at home and school is essential to minimize deleterious responses to eye trauma.

Aim To investigate prognostic significance of preoperative levels of the Carbohydrate anti-gen 19-9 (CA 19-9) in patients with stage III rectal adenocarcinoma who underwent a treatment at the Clinical Centre of the University of Sarajevo. Materials A retrospective cohort study included 84 patients who underwent radical anterior rectal resection due to grade III rectal adenocarcinoma, followed by adjuvant chemotherapy according to the FOLFOX protocol (Oxaliplatin, Leucovorin, 5-Fluorouracil (5-FU)). The patients were divided into two groups according to CA 19-9 values (≥27 U/mL and <27 U/mL, respectively). Results High pre-operative CA 19-9 values predicted an increased probability of postoperative metastases, especially liver, lung and abdominopelvic metastases, as well as three-year disease-free survival (3Y-DFS) and three-year overall survival (3Y-OS). The 3Y-DFS rate for patients with high CA 19-9 was 64.5%, while for those with low CA 19-9 it was 87.2%. The 3Y-OS rate for patients with high CA 19-9 was 89.8%, while for those with low CA 19-9 it was 65.7%. Univariate and multivariate regression analysis confirmed that a high level of CA 19-9 is an independent predictor for DFS and OS shorter than three years. Conclusion Pre-operatively elevated values of CA 19-9 in rectal adenocarcinoma have a significant role in predicting the outcome in patients with stage III rectal adenocarcinoma.

Simple Summary Achieving margins of >5 mm is challenging in the larynx and hypopharynx because resections are constrained by their complex anatomy. The aim of this study was to retrospectively assess the clinical relevance of resection margins defined by the RCP in total laryngectomies (TLs) and total laryngopharyngectomies (TLPs). Similar survival rates for close and clear margins for primary and recurrent LSCC were found. This may suggest that a margin > 5 mm is not clinically relevant in terms of survival, therefore a margin of 1–5 mm should be accepted in certain subsites. Margins < 1 mm are related to significantly worse outcomes and should be avoided. Abstract Background: Laryngeal and hypopharyngeal cancer is complex and resection margins are therefore constrained. The aim of this study was to investigate the clinical relevance of resection margins in laryngeal and hypopharyngeal surgery. Methods: A retrospective cohort study was performed for patients treated with a total laryngectomy (TL) or laryngopharyngectomy (TLP) for laryngeal or hypopharyngeal squamous cell carcinoma (LSCC and HSCC, respectively). Within the groups primary LSCC, recurrent LSCC, primary HSCC, and recurrent HSCC the relationship between the status of the resection margin according to the Royal Collage of Pathology and the recurrence and survival rates were investigated. Results: Positive resection margins were found in 54% for primary LSCC, 29% for recurrent LSCC, 62% for primary HSCC, and 44% for recurrent HSCC. For primary and recurrent LSCC, there was a linear association between total recurrence and narrowing margins (p = 0.007 resp. p = 0.008). Multivariate survival analysis for primary and recurrent LSCC showed a significantly worse disease free and disease-specific survival in case of positive margins compared to clear margins. Conclusion: Similar survival rates were recorded for close and clear margins for primary and recurrent LSCC. This may suggest that a margin > 5 mm is not clinically relevant in terms of survival. Therefore, a margin of 1–5 mm should be accepted in certain subsites. Margins < 1 mm are related to significantly worse outcomes and should be avoided.

This paper presents a system that is able to detect physical intrusion in a specific space based on temperature and humidity change. This specific space was housing hardware components important for information security management infrastructure. Presented system is able to predict that two spaces are connected and that there is a physical breach in protected space. The presented prediction approach involves identifying patterns in historical data, where the subsequent outcomes are already known in advance, and validating these patterns using more recent data. System is implemented using k-Nearest Neighbours, Random Forest, and Support Vector Machine algorithms in Python programming language on Raspberry Pi. Real observed data to predict if specific temperature and humidity indicates intrusion were used. This approach can be used to detect intrusions in the room or in other closed space. More specifically thermal equilibrium phenomenon between two spaces after barrier between them are opened was monitored. Through process of supervised learning using labelled data, system was able to detect intrusion by using k-nearest neighbours, random forest, and support vector machine with different accuracy. Presented model shows better results using k-nearest neighbours and support vector machine with accuracy of 100% compared to random forest with accuracy of 95%. The system is low cost because of cheap Raspberry Pi controller and sensors.

Antibiotics are medications that inhibit bacterial growth and are used in the treatment of bacterial infections, both gram-positive and gram-negative. Antibiotics also represent products of metabolism of certain microorganisms that adversely affect the development and reproduction of other microorganisms. They are one of the greatest discoveries of modern medicine; however, in today's age, the greatest challenge is the resistance of bacteria to antibiotics. There are numerous reasons for the emergence of antibiotic resistance. One reason lies in inadequate education about antibiotics, their application, and usage. The aim of this study was to survey students of the University of Sarajevo's Faculty of Health Sciences about their general knowledge of antibiotics and their methods of application. A total of 151 students participated in this study, including 125 females (83%) and 26 males (17%). The questions consisted of two parts: the first part covered general knowledge about antibiotics, while the second part addressed bacterial resistance to antibiotics.

Approximately half of the cases of chronic kidney disease (CKD) in childhood are caused by congenital anomalies of the kidney and urinary tract (CAKUT). Specific genes were identified as having significant importance in regard to the underlying genetic factors responsible for the CAKUT phenotype, and in our research, we focused on analyzing and comparing the expression levels of ectodysplasin A2 receptor (EDA2R), protocadherin9 (PCDH9), and TNF receptor-associated factor 7 (TRAF7) proteins in the cortex and medulla of healthy control kidneys during developmental phases 2, 3, and 4. We also performed an analysis of the area percentages of the mentioned proteins in the cortical and medullary sections of healthy embryonic and fetal kidneys compared to those affected by CAKUT, including duplex kidneys (DK), horseshoe kidneys (HK), hypoplastic kidneys (HYP), and dysplastic kidneys (DYS). We found that the CAKUT candidate gene proteins EDA2R, PCDH9, and TRAF7 are all expressed during normal human kidney development stages. In DYS, the expression of EDA2R was higher than in normal kidneys, likely due to EDA2R’s role in apoptosis, which was upregulated in specific cases and could possibly contribute to the formation of DYS. The expression of PCDH9 was lower in HK, which can be attributed to the possible role of PCDH9 in cell migration suppression. Decreased PCDH9 expression is linked to increased cell migration, potentially contributing to the development of HK. The level of TRAF7 expression was reduced in all examined kidney disorders compared to normal kidneys, suggesting that this reduction might be attributed to the crucial role of TRAF7 in the formation of endothelium and ciliogenesis, both of which are essential for normal kidney development. Further research is required to ascertain the function of these proteins in both the typical development of the kidney and in CAKUT.

Background and Objectives: Spinal surgery, particularly for cervical pathologies such as myelopathy and radiculopathy, requires a blend of theoretical knowledge and practical skill. The complexity of these conditions, often necessitating surgical intervention, underscores the need for intricate understanding and precision in execution. Advancements in neurosurgical training, especially with the use of low-cost 3D models for simulating cervical spine tumor removal, are revolutionizing this field. These models provide the realistic and hands-on experience crucial for mastering complex neurosurgical techniques, filling gaps left by traditional educational methods. Materials and Methods: This study aimed to assess the effectiveness of 3D-printed cervical vertebrae models in enhancing surgical skills, focusing on tumor removal, and involving 20 young neurosurgery residents. These models, featuring silicone materials to simulate the spinal cord and tumor tissues, provided a realistic training experience. The training protocol included a laminectomy, dural incision, and tumor resection, using a range of microsurgical tools, focusing on steps usually performed by senior surgeons. Results: The training program received high satisfaction rates, with 85% of participants extremely satisfied and 15% satisfied. The 3D models were deemed very realistic by 85% of participants, effectively replicating real-life scenarios. A total of 80% found that the simulated pathologies were varied and accurate, and 90% appreciated the models’ accurate tactile feedback. The training was extremely useful for 85% of the participants in developing surgical skills, with significant post-training confidence boosts and a strong willingness to recommend the program to peers. Conclusions: Continuing laboratory training for residents is crucial. Our model offers essential, accessible training for all hospitals, regardless of their resources, promising improved surgical quality and patient outcomes across various pathologies.

This paper presents an in-depth analysis of the Kopčić House, a significant example of modernist architecture in Sarajevo, Bosnia and Herzegovina, focusing on its structural-specific features and seismic performance. The Kopčić House embodies a confined masonry structure with innovative construction features, combining load-bearing masonry walls with reinforced concrete elements. This architectural approach was pioneering for its time, combining traditional construction methods with innovative materials and techniques. Detailed analysis using numerical modeling techniques, specifically 3D modeling with the 3Muri software (Vers.14.2.0.4), was conducted to assess the seismic resilience of the structure. The analysis considered different load distributions and eccentricities to comprehensively evaluate the building’s response to lateral forces. The findings of this research reveal the structural capacity and potential vulnerabilities of the Kopčić House when subjected to seismic events. While the building demonstrates inherent strength due to its confined masonry design, areas requiring structural strengthening were identified through numerical simulations. This study contributes to the broader understanding of confined masonry construction within the context of modernist architecture. By integrating historical research with advanced structural analysis, this work aims to bridge the gap between architectural heritage and contemporary engineering practices.

Simple Summary This review assesses the integration of nanotechnology and immunotherapy, with a specific focus on the utilization of nanomaterials to modulate the immune system in conditions such as cancer and autoimmunity. Liposomes, polymers, and inorganic nanoparticles (NPs) are versatile nanomaterials capable of effectively transporting immunomodulatory molecules. Their interactions with immune cells have contributed to the development of checkpoint inhibitors, improved cancer vaccines, and the optimization of adoptive cell therapies. These approaches enable the fine-tuning of immune responses with minimal adverse effects. Positioned at the forefront of the convergence of nanotechnology and immunology, nanomaterial-based platforms have the potential to revolutionize patient-centered immunotherapy. These systems are used in the transformative era of disease management by prioritizing safety, personalization, and compliance with regulations. Abstract Immunotherapy is a rapidly advancing field of research in the treatment of conditions such as cancer and autoimmunity. Nanomaterials can be designed for immune system manipulation, with precise targeted delivery and improved immunomodulatory efficacy. Here, we elaborate on various strategies using nanomaterials, including liposomes, polymers, and inorganic NPs, and discuss their detailed design intricacies, mechanisms, and applications, including the current regulatory issues. This type of nanomaterial design for targeting specific immune cells or tissues and controlling release kinetics could push current technological frontiers and provide new and innovative solutions for immune-related disorders and diseases without off-target effects. These materials enable targeted interactions with immune cells, thereby enhancing the effectiveness of checkpoint inhibitors, cancer vaccines, and adoptive cell therapies. Moreover, they allow for fine-tuning of immune responses while minimizing side effects. At the intersection of nanotechnology and immunology, nanomaterial-based platforms have immense potential to revolutionize patient-centered immunotherapy and reshape disease management. By prioritizing safety, customization, and compliance with regulatory standards, these systems can make significant contributions to precision medicine, thereby significantly impacting the healthcare landscape.

The design process of motor yachts mainly relies on the experience of designers, who have confidence in the knowledge acquired from designing units with similar hull-form characteristics. However, once a new concept needs to be developed, the acquired experience on a standard platform is no longer sufficient to achieve in a short time a successful design. The design of a motor yacht implies considering multiple aspects of ship hydrodynamics: resistance, propulsion, seakeeping, and manoeuvring. Such factors have been widely discussed individually on different kinds of ships, but an appropriate joint investigation of hulls like motor yachts is missing in the open literature. Therefore, the present paper intends to cover this gap, providing guidelines for the design of motor yachts in a length range between 20 and 40 meters. As a preliminary study, a series of 15 yacht hulls has been developed, starting from a reference hull form. Seakeeping, manoeuvring, and propulsive performances have been evaluated at a reference environmental condition and speeds according to the ISO 22834:2022 guidelines. Such calculations allow for developing response surfaces of the hydrodynamic properties for the series of yachts as a function of the hull's main dimensions. As a final result, the obtained responses allow for identifying the best compromise solutions for the main dimension selection of a new motor yacht in the length range of 20-40 meters.

Adhesive bonding has proven to be a reliable method of joining materials, and the development of new adhesives has made it possible to use bonding in a variety of applications. This article addresses the challenges of bonding metals such as the aluminum alloy EN AW-5754 and the stainless steel X5CrNi18-10. In this study, the effects of laser cleaning and texturing on the surface properties and strength of two bonded joints were investigated and compared with mechanical preparation (hand sanding with Scotch-Brite and P180 sandpaper). The bonded joints were tested with three different epoxy adhesives. During the tests, the adhesion properties of the bonded surface were determined by measuring the contact angle and assessing the wettability, the surface roughness parameters for the different surface preparations, and the mechanical properties (tensile lap-shear strength). Based on the strength test results, it was found that bonded joints made of stainless steel had 16% to 40% higher strength than aluminum alloys when using the same adhesive and surface preparation. Laser cleaning resulted in maximum shear strength of the aluminum alloy bond, while the most suitable surface preparation for both materials was preparation with P180 sandpaper for all adhesives.

Introduction: Falls in the elderly population are one of the main geriatric syndromes and a clear indicator of the fragility of the elderly population. Falls are a leading cause of death and injury in the elderly, resulting in disability and immobility requiring ongoing medical care and high treatment costs. Difficulties in performing daily life activities increase with age due to decline in physical and cognitive functioning. Research objectives: To assess the ability to perform daily life activities, to analyze the risks of falling in elderly people. Methods: 100 people over the age of 65 participated in the research. The research instruments were a questionnaire for the assessment of daily life activities - Barthel Scale / Index (BI) and a questionnaire on screening assessments for the evaluation of falls. Results: The Barthel index shows that the majority of respondents perform daily life activities without problems. Intrinsic factors prove that the median score was 12 with an interquartile range of 7 to 15, which represents the risk of falls in people of the third age. Extrinsic risk factors for falls in people of the third age indicate a median of 4 with an interquartile range of 2 to 5, which represents the risk for falls in older people. The most influential risk factors for falling in our survey are difficulty walking upstairs, incorrect or insufficient use of orthopedic aids, feeling of instability when standing up for the first time, complaining of weakness or reduced sensation in one or the other leg, feeling of low self-confidence, instability when walking and fear from falling. Conclusion: Analyzing the risk factors for a fall, we obtained the result that there is an evident risk for a fall. Based on the estimated risk of falling, the most influential risk factors that affect the performance of daily life activities of elderly people were singled out.