Visual Abstract The auditory brainstem response (ABR) is a measure of subcortical activity in response to auditory stimuli. The wave V peak of the ABR depends on the stimulus intensity level, and has been widely used for clinical hearing assessment. Conventional methods estimate the ABR average electroencephalography (EEG) responses to short unnatural stimuli such as clicks. Recent work has moved toward more ecologically relevant continuous speech stimuli using linear deconvolution models called temporal response functions (TRFs). Investigating whether the TRF waveform changes with stimulus intensity is a crucial step toward the use of natural speech stimuli for hearing assessments involving subcortical responses. Here, we develop methods to estimate level-dependent subcortical TRFs using EEG data collected from 21 participants listening to continuous speech presented at 4 different intensity levels. We find that level-dependent changes can be detected in the wave V peak of the subcortical TRF for almost all participants, and are consistent with level-dependent changes in click-ABR wave V. We also investigate the most suitable peripheral auditory model to generate predictors for level-dependent subcortical TRFs and find that simple gammatone filterbanks perform the best. Additionally, around 6 min of data may be sufficient for detecting level-dependent effects and wave V peaks above the noise floor for speech segments with higher intensity. Finally, we show a proof-of-concept that level-dependent subcortical TRFs can be detected even for the inherent intensity fluctuations in natural continuous speech.

Objective: This comprehensive research aimed to thoroughly examine the effectiveness of a diode laser (445 nm) in combination with non-surgical treatment in patients with chronic periodontitis (CP) by evaluating a wide range of clinical and microbiological parameters. Materials and methods: Thirty-one subjects diagnosed with CP were included in this study. The total number of treated periodontal pockets was 862. The subjects were randomly assigned to group 1, which underwent scaling and root planing and laser therapy (SRP+L), and group 2, which underwent scaling and root planing (SRP) only. All respondents underwent a periodontal diagnostic protocol. The parameters plaque index (PI), gingival index (GI), bleeding on probing index (BOP), probing depth (PD), clinical attachment level (CAL), and tooth mobility (TM) were registered. Clinical periodontal measurements were performed at baseline and one and three months after therapy. Microbiological analysis was conducted on Aggregatibacter actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg), and Tannerella forsythia (Tf) using real-time polymerase chain reaction (PCR). For microbiological analysis, samples were taken at baseline, immediately after therapy, and after three months. Laser irradiation was performed immediately after SRP. Results: All clinical parameters improved statistically from baseline to three months after therapy. For all examined clinical parameters, better results were achieved in group 1 than in group 2. This study showed a more significant reduction in Pg and Tf from baseline to three months in group 1 compared to group 2. Conclusion: These results showed that the diode laser wavelength 445 nm was also usable in treating periodontal diseases as an additional method to SRP.

Aim To assess the relationship between the attitudes of general practitioners/family medicine doctors (GP/FD) and of their patients toward industry-sponsored clinical research. Methods A cross-sectional survey included volunteer GPs/FDs who then enrolled and interviewed their patients. Data were analyzed in hierarchical models (patients nested in GPs/FDs, nested in countries/regions). Results A total of 201 GPs/FDs from nine European countries responded to the invitation and enrolled 995 of their patients. We observed mild associations between some of the GPs/FDs’ attitudes (general opinion on sponsored clinical studies, appreciation of the general values of such studies, views about the importance of participant protection/privacy) and some of the patients’ attitudes (appreciation of the general values and of risks associated with sponsored clinical studies, importance assigned to potential personal benefits from participation). We observed no association between GPs/FDs’ attitudes and patients’ willingness to participate in such studies. However, willingness to participate increased with higher patients’ appreciation of the general values of sponsored studies, decreased with higher patients’ appreciation of associated risks, and showed a quadratic trend across the levels of importance assigned by patients to potential personal benefits (willingness was higher when the assigned importance was very low or very high). More importance to GP/FD’s advice in this respect was assigned by patients who assigned more importance to potential personal benefits, who were better educated, and who resided in rural/suburban dwellings. Conclusions In the present convenience sample, lay-person attitudes about and willingness to participate in industry-sponsored clinical studies were associated with the attitudes of their GPs/FDs.

Polyphenols are abundant natural plant micronutrients that commonly contribute to human health due to their anti-inflammatory, antioxidant, antiviral, anti-carcinogenic, anti-aging, anti-allergic, and other biological activities. Their therapeutic benefits mainly depend on the structure, stability, chemical interactions, and absorption, which ultimately affect the bioavailability of these compounds. The bioactivity of polyphenols is evaluated by in vitro and in vivo studies, sometimes yielding inconsistent results due to numerous differences between used models. Among the main differences is the production of reactive oxygen species (ROS) in cultured cell models, potentially leading to misinterpretation of the effects of polyphenolic compounds. Little attention is paid to the polyphenol stability in cell culture medium and the potential generation of artifacts due to their chemical instability. Stability tests of polyphenols are strongly advised to be performed in parallel with cell culture, to help avoid misleading conclusions. This review highlights the existing challenges with cell-based research, focusing on polyphenols' stability in the cell culture media. We also emphasize that new methods analyzing the molecular interactions of compounds with cell culture media supplements are essential to provide a comprehensive understanding of the polyphenols in in vitro models.

Carnassial teeth, as one of the most important teeth in dogs, are commonly affected by different types of injuries and pathological disorders, which require endodontic treatment. Lateral canals, which leave the main root canal, and apical deltas are present in the root canal system of dogs. These canals represent small branches that leave the main root canal at a sharp angle and terminate in a region of the periodontal ligament on the external root surface. In this study, 200 teeth were included, of which 100 were superior fourth premolars and 100 were inferior first molars. Lateral canals were present in a total of 30 roots of superior fourth premolar teeth, whereas in inferior first molars, these canals were found in 3% of roots. In superior fourth premolars, lateral canals were mostly present in the apical third, 86%, while 14% were present in the middle third of the root. In inferior first molars, lateral canals were found in 3% of the roots, 2% in the apical third and 1% in the middle third of the roots. The knowledge of the complex root canal morphology is essential for any endodontic treatment. Due to the presence and frequency of lateral canals, it is imperative to perform more thorough cleaning and instrumentation while performing endodontic treatment on superior fourth premolars compared to inferior first molars.

Abstract Energy production, supply and consumption are global issue with many economic, environmental and social implications. Mentioned issue is even more expressed in remote rural areas, in particular in developing countries, as are the countries of the Western Balkans (WB). Renewable energy sources (RES) could represent optimal energy alternative for sustainable performing of agricultural and other activities, as well as for improving the current state of living conditions in rural communities. The main goal of research is to mark the most suitable RES alternative (six alternatives) for wider implementation in rural space of WB. The applied methodology framework implies experts’ opinion (engagement of eight experts) and the use of multi-criteria decision-making methods (MCDM), (specifically fuzzy-rough LMWA and fuzzy-rough CRADIS methods) under the predefined criteria (nine criteria). Derived results show that the implementation of the solar energy plants could play an optimal solution, while as the relatively unsuitable alternative could be marked the use of energy potential of watercourses. Gained final result, i.e. ranking order of the considered alternatives is additionally verified by the appliance of other MCDM methods, while the sensitivity analysis was also performed.

In this research is presented a new method for determining the weights of criteria called simple weight calculation (SIWEC) method. The steps of this method are presented in the practical example of determining the importance of criteria for the needs of sales of agricultural products in the Semberija region. During the presentation of this method two methods are elaborated the simple SIWEC method which includes numerical ratings and the fuzzy SIWEC method which includes ratings in the form of linguistic value. In the selected example is presented how to use this method in order to determine the importance of criteria and in both cases the criterion of sales reliability is given the greatest weight. The contribution SIWEC method is reflected in its simplicity, which facilitates decision-making.• The method presented in this research apart from others is that it uses the evaluation of the criteria by decision makers, so the criteria should not be ranked and compared, but simply evaluated.• Unlike similar methods, the presented method uses the adjusted steps of the method for ranking the alternatives, and decision makers are given a different importance in the decision-making.

Hodgkin lymphoma (HL) is a rare lymphoid neoplasm in which Hodgkin/Reed–Stenberg (HRS) cells are admixed with a population of non-neoplastic inflammatory cells and fibrosis. Dysregulated expressions of cell cycle regulators and transcription factors have been proven as one of the hallmarks of HL. In that context, SATB1 and p16 have been reported as potential regulators of HL progression and survival. However, to date, no studies have assessed the expression levels of SATB1 and p16 in HL in Croatian patients or their prognostic values. Therefore, we investigated the expression pattern of SATB1 and p16 in paraffin-embedded lymph node biopsies using standard immunohistochemistry. We found that 21% of the patients stained positive for SATB1, while 15% of the patients displayed positive staining for p16. Furthermore, we aimed to understand the prognostic value of each protein through the analysis of the overall survival (OS) and progression-free survival (PFS). SATB1 showed a significantly positive correlation with better OS and PFS, while p16 expression had no impact. Interestingly, when patients were stratified by a combination of the two studied markers, we found that patients in the SATB1+/p16- group tended to have the best prognosis in HL, according to statistical significance. In conclusion, SATB1 and p16 might be potentially useful as diagnostic and prognostic markers for HL.

The association between movement screening and physical fitness testing in athletes is conflicting, and therefore, this study aimed to examine the relationship between Functional Movement Screen (FMS) performance and physical performance in elite female youth football players. Twenty-two players from the national U16 team of Bosnia and Herzegovina underwent FMS and physical performance tests, including speed, agility, and jump assessments. Jump and speed performance score correlated well with ASLR, while the overall FMS score was not associated with any of the performance variables. These findings suggest that while certain movement patterns may impact athletic performance, the relationship between movement screening and physical performance is delicate. Coaches and practitioners should consider individual variations and sport-specific demands when interpreting FMS results in order to optimize and maximize athlete performance and reduce injury risks.

This study investigates the impact of infill density on the mechanical properties of fused deposition modeling (FDM) 3D-printed polylactic acid (PLA) and PLA reinforced with carbon fiber (PLA+CF) specimens, which hold industrial significance due to their applications in industries where mechanical robustness and durability are critical. Exposure to cooling lubricants is particularly relevant for environments where these materials are frequently subjected to cooling fluids, such as manufacturing plants and machine shops. This research aims to explore insights into the mechanical robustness and durability of these materials under realistic operating conditions, including prolonged exposure to cooling lubricants. Tensile tests were performed on PLA and PLA+CF specimens printed with varying infill densities (40%, 60%, 80%, and 100%). The specimens underwent tensile testing before and after exposure to cooling lubricants for 7 and 30 days, respectively. Mechanical properties such as tensile strength, maximum force, strain, and Young’s modulus were measured to evaluate the effects of infill density and lubricant exposure. Higher infill densities significantly increased tensile strength and maximum force for both PLA and PLA+CF specimens. PLA specimens showed an increase in tensile strength from 22.49 MPa at 40% infill density to 45.00 MPa at 100% infill density, representing a 100.09% enhancement. PLA+CF specimens exhibited an increase from 23.09 MPa to 42.54 MPa, marking an 84.27% improvement. After 30 days of lubricant exposure, the tensile strength of PLA specimens decreased by 15.56%, while PLA+CF specimens experienced an 18.60% reduction. Strain values exhibited minor fluctuations, indicating stable elasticity, and Young’s modulus improved significantly with higher infill densities, suggesting enhanced material stiffness. Increasing the infill density of FDM 3D-printed PLA and PLA+CF specimens significantly enhance their mechanical properties, even under prolonged exposure to cooling lubricants. These findings have significant implications for industrial applications, indicating that optimizing infill density can enhance the durability and performance of 3D-printed components. This study offers a robust foundation for further research and practical applications, highlighting the critical role of infill density in enhancing structural integrity and load-bearing capacity.