The John Cunningham virus (JCV) is a polyomavirus that usually infects people at a young age and does not cause any symptoms in immunocompetent individuals. However, in immunocompromised individuals, such as kidney transplant recipients, JCV can cause severe and potentially fatal disease. Unfortunately, JCV has not been researched as extensively as the BK virus and is not mentioned in relevant kidney transplant guidelines. This lack of attention to JCV can lead to less consideration in kidney transplant patients’ care. Surveillance using locally available diagnostic methods is of the utmost importance. The presence of JCV can be diagnosed with urine decoy cells, viruria, or viremia verified by the PCR method. A low threshold for considering JCV as a possible cause of any neurological or renal dysfunction in kidney transplant recipients must be maintained. In such cases, kidney and brain biopsy are indicated. Maintaining the appropriate immunosuppression while avoiding over-immunosuppression to prevent JCV disease is crucial, and the approach should be individual, according to overall immunological risk. We hypothesize that the presence of the JCV can indicate overt immunosuppression and identify kidney transplant recipients more prone to opportunistic infections and diseases, including some malignancies. To explore that, future observational studies are needed.

Bryophytes are a group of plants vital to many ecosystems and biogeochemical cycles and are well known bioindicators and biomonitoring tools. However, they have been deemed industrially less important than vascular plants and their potential for applications other than as biomonitoring tools has been overlooked. In recent years, however, bryophytes, and in particular mosses, are starting to gain attention as viable phytoremediation agents. Studies indicate that some moss species have the ability to uptake heavy metals such as Pb, Cd, Zn, Cu, As, and Cr from contaminated water. Tested mosses could both adsorb and absorb significant amounts of specific heavy metals without adverse effects on the plant. Results suggest that moss biomass, either dry or wet, could be used as a bio-sorbent in filtration of heavy metals. The review of available literature shows a promising prospect of moss to be used in phytofiltration of heavy metals. Existing knowledge on this topic could be the basis for further research which isneeded. KEYWORDS:moss; heavy metal; phytofiltration; bio-sorbent

Livestock production is a potential environmental pollutan because of the high concentartions of animals on the small area. Production of biogas with anaerobic degradation from organic waste is one of the pledge alternative energetic solutions, especially from organic manure made from animal farming and other residuals of agricultural production.The aim of this paper was to determine posibillity of using manure different origin from extensive animal breeding for production of biogas in labaratory conditions. The results obtained will be based on posibillity of use waste streams from extensive animal husbandry as basic substrate for anaerobic digestion.In this regard, cosubstrates were formed as mixture 1 chicken excrement; mixture 2 sheep manure; mixture 3 cow manure; all three basic substrates were in mixture with the sludge from the wastewater treatment plant. The results showed production of 71,5 ml CH4/gVS for mixture 1, 68,65 ml CH4/gVS for mixture 2 and 48,68 ml CH4/gVS for mixture 3. KEYWORDS:extensive breeding, manure, anaerobic digestion, biogas, biomethane potentional

Nowadays, bottled water has become part of the lifestyle, replacing tap water, and water from freestanding dispensers is increasingly being used. The main goal of this research was to determine the health suitability of bottled water in freestanding dispensers. The research included the microbiological analysis of a total of 100 samples of bottled water from freestanding dispensers. The samples were mostly taken in the wider area of the city of Doboj (doctor's offices, shops, public buildings), and one dispenser in the city of Tuzla. Water samples were taken twice, in the period from April to June 2022. Water samples from 6 different manufacturers (Vivia, Kristal, Nevra, Gora, Aqua doria, Aqua team) were analyzed. 9% of water samples (9/100) were microbiologically correct. Microbiologically defective samples contained a higher total number of bacteria at 22°C and 37°C, as well as a higher number of coliform bacteria than the maximum allowed values. No significant differences in microbiological quality were found between older and/or recently installed water dispensers, as well as in terms of environmental conditions, while visible differences were observed between dispensers that were regularly hygienically maintained.KEYWORDS:dispensers; bottled water; coliform bacteria; biofilms; total bacteria count

– In this paper, the influence of waste shear strength parameters on landfill slope stability is studied. Namely, using the limit equilibrium method in GeoStudio 2018, stability analyses of a typical landfill slope are performed using semi-probabilistic and probabilistic approaches. The semi-probabilistic computations of slope stability are performed based on the available literature recommendations for the waste shear strength parameters. The results obtained for different recommendations are compared and discussed. In the probabilistic computations, the waste shear strength parameters are treated as random variables with Gaussian random distribution, where the parameters of the distribution are again selected based on the literature recommendations. Here, the influence of dispersion in the values of waste shear strength parameters is also examined. In addition, sensitivity analyses are also performed to gain insights into the relative importance of parameters. With the aforementioned analyses, an effort was made to investigate and derive conclusions about how the selection of waste shear strength parameters affects landfill slope stability.

The aim of this paper is focused on reducing sodium chloride content by partial replacement with potassium chloride and magnesium chloride in cooked cheese samples. For the production of cheese, standardized cow's milk from a domestic market producer was used, and the cheese was produced by heating the milk to a temperature of 95°C and coagulation with acetic acid. The one salted only with NaCl was designated as the standard sample, and the other samples were salted with combinations of salts in which NaCl reduction was performed: sample A1 had a ratio of 15% KCl:85% NaCl, sample A2 30% KCl:70% NaCl, sample B1 15% MgCl2:85% NaCl and sample B2 30% MgCl2:70% NaCl. The cheese samples were stored at + 4°C and color parameters and sensory properties were analyzed on the 1st, 3rdand 5thdays of storage. Based on the performed analyses, it was concluded that it is completely acceptable to replace sodium chloride with potassium chloride in the ratio of 15% KCl:85% NaCl. It is acceptable to replace sodium chloride with potassium chloride inthe ratio of 30% KCl:70% NaCl, with the note that on the 5thday of storage there is a gradual deterioration of the sensory properties compared to the samples analyzed on the 1stday of storage. Replacement of sodium chloride with magnesium chloride in the ratios 15% MgCl2:85% NaCl and 30% MgCl2:70% NaCl is not acceptable.As such, it is not recommended in the production of cooked cheeses due to the appearance of a metallic and bitter taste that is present in cheese samples from the 1st to the 5th day of storage. KEYWORDS:cooked cheese, sodium chloride, potassium chloride, magnesium chloride

Objectives: The aim of this study was to determine the short-term consequences of coronavirus disease 2019 (COVID-19) infection on pulmonary diffusion in patients with severe (but not critical) and moderately severe COVID-19 pneumonia during three months after COVID-19 infection. Methods: A prospective study included 81 patients with an RT-PCR-test confirmed diagnosis of COVID-19 infection treated in the COVID Department of Lung Diseases of University Clinical Hospital Mostar. Inclusion criteria were ≥18-year-old patients, COVID-19 infection confirmed using real-time RT-PCR, radiologically confirmed bilateral COVID-19 pneumonia, and diffusion capacity of the lungs for carbon monoxide (DLCO) one and three months after COVID-19 infection. The pulmonary function was tested using the MasterScreen Body Jaeger (Jaeger Corporation, Omaha, USA) and MasterScreen PFT Jaeger (Jaeger Corporation, Omaha, USA) according to American Thoracic Society guidelines one and three months after COVID-19 infection. Results: Forced vital capacity significantly increased three months after COVID-19 infection compared to the first-month control (p<0.0005). Also, a statistically significant increase in the FEV1 value (p<0.0005), FEV1%FVC ratio (p<0.005), DLCO/SB (p<0.0005), DLCO/VA value (p<0.0005), and total lung capacity (TLC) (p<0.0005) was observed in all patients. Conclusion: Our study showed that recovery of DLCO/VA and spirometry parameters was complete after three months, while DLCO/SB was below normal values even after three months. Therefore, one month after the COVID-19 infection patients had partial recovery of lung function, while a significant recovery of lung function was observed three months after the COVID-19 infection.

One of the main causes of the dismal prognosis in patients who survive the initial bleeding after aneurysmal subarachnoidal hemorrhage is the delayed cerebral ischaemia caused by vasospasm. Studies suggest that cerebral magnesium and pH may potentially play a role in the pathophysiology of this adverse event. Using phosphorous magnetic resonance spectrocopy (31P-MRS), we calculated the cerebral magnesium (Mg) and pH levels in 13 patients who suffered from aSAH. The values between the group that developed clinically significant vasospasm (n = 7) and the group that did not (n = 6) were compared. The results of this study show significantly lower cerebral Mg levels (p = 0.019) and higher pH levels (p < 0.001) in the cumulative group (all brain voxels together) in patients who developed clinically significant vasospasm. Further clinical studies on a larger group of carefully selected patients are needed in order to predict clinically significant vasospasm.

This paper presents a multi-criteria decision-making model based on the application of two methods, DIBR II and MABAC. The DIBR II method was used to define weight coefficients. The MABAC method was used to rank alternatives, and it was applied in a rough environment. Four experts were engaged in defining the criteria and alternatives as well as in the relation of criteria. The model was applied for ranking the methods and techniques of Lean organization systems management in the maintenance of technical systems of special purposes. At the end of the application was conducted a sensitivity analysis which proved the stability of the obtained results.