Purpose. The aim of the study was to examine the effects of five weeks detraining period on body composition changes in elite professional Indonesian male soccer players. Material and methods. The body composition of 24 top-level male soccer players (age: 26.33 ± 4.06 years) competing in First League of Indonesia was measured before and after the 5-weeks detraining period. Body composition variables, including body mass, body fat percentage, fat-free mass, and total body water percentage, were measured using the bioelectrical impedance method. The results. The paired samples T-test showed significant changes in the means of body composition variables for body fat percentage, fat-free mass, and total body water percentage. Mean values for body fat percentage increased during the detraining period from 10.87 ± 2.47% to 11.98 ± 2.75% (10.3%). A decrease in mean values for fat-free mass (61.34 ± 5.74 kg to 59.80 ± 6.22; 2.49%) and total body water percentage (from 65.13 ± 1.84% to 64.40 ± 1.99%; 1.12%) was observed. No significant changes occurred in body mass (from 69.05 ± 8.07 kg to 69.05 ± 8.48 kg; 0.04%) from initial to final testing. Conclusions. Since there was a significant difference in body fat percentage, fat-free mass, and total body water percentage% before and after detraining period., it is suggested that 5 weeks of detraining period.  for the professional soccer players may not be necessary, and the duration should be shortened to maintain body composition status.

Abstract Objectives To develop two ethical checklists to evaluate (i) management of ethical concerns in research projects and (ii) awareness of ethical conduct of healthcare laboratory professionals. Methods Comprehensive discussion among the members of IFCC Task Force on Ethics based on pertinent literature. Results This Checklist for Clinical Research Projects should be useful to evaluate research proposals from an ethical perspective before submitting it to an IRB or its equivalent, thereby diminishing rejection rates and resulting in more time-effective projects. The checklist designed to evaluate the ethical conduct in laboratory medicine could be useful for self evaluation (internal audits) and for certification/accreditation processes performed by third parties. Conclusions These checklists are simple but powerful tools useful to guide professionals to adhere to ethical principles in their practice, especially in developing countries where accredited ethics committees may be difficult to find.

To investigate a robot’s role in children’s welfare and safety, we conducted interviews with 8 Subject Matter Experts and Professionals (SMEs) across the disciplines of robotics, child technology, psychology, and psychiatry disciplines. Through qualitative analysis, we synthesize the challenges of safeguarding, compounding limitations, and potential solutions for involving robots in safeguarding as broadly defined in SME interviews. While they agree robots should not be responsible for making judgement calls, the experts also identified the ways robots can be a valuable addition to the safeguarding team. However, more conversations spanning disciplines need to occur to inform policy and legal frameworks that will better establish a robot’s role in intimate spaces in children’s lives. While this line of inquiry is specific to robots in safeguarding, many of the themes reflect the nuances of finding appropriate places for child-robot interactions in the context of children’s welfare.

Introduction: Acute lymphoblastic leukemia (ALL) is the most frequent pediatric leukemia; it can be defined according to chromosomic and genomic data. Cytogenetic analyses and determination of chromosomal numbers (such as hypo- or hyperdiploidy) and/or specific chromosomal rearrangements are basic for ALL classification and treatment. Even though cure rates of childhood ALL are at ~95%, pharmacogenetic aspects are of raising importance. Material and Methods: We have analyzed the literature for ALL subtypes, corresponding therapy options, and pharmacogenetic implications. Results: Data for ALL subtypes such as B-ALL, T-ALL, Ph-like ALL, DS-ALL, ETP-ALL, BCR-ABL1-like ALL are presented here. The gene polymorphism which lead to metabolizability of 6-MP are ITPA variants (94C>A) and IVS2+21A>C, in conjunction with TPMT (238G>C, TPMT*3B 460G>A and *3C 719A>G and NUDT15 (415C>T). For methotrexate metabolism gene polymorphisms are found for gene MTHFR as C677T and A1298C. Conclusion: In the last decade in many hospital laboratories, pharmacogenetic aspects gain more and more importance. Application of many molecular biology methods provided progress in treatment and diagnosis of ALL patients. Combination therapy is proposed as an alternative to single drug treatments.

The Internet of Things (IoT) is one of the key technologies for the digital transformation of both business and society. The paper primarily aims to investigate the structure and dynamics of academic publications in English dealing with issues of IoT development – from a marketing perspective, using methods of systematic mapping, i.e., co-citation analysis, bibliographic coupling, and analysis of the co-occurrence of keywords in the Scopus and Web of Science (WoS) databases. Analysis of research papers enables the identification of the most influential articles, papers, and journals, as well as visualization of the keywords and co-authorship co-occurrences. There has been an exponential increase in studies on IoT applications in marketing, indexed in Scopus and WoS citation databases. An insight into analyzed papers shows that companies utilize large amounts of data generated by the IoT to gain insights into customers’ supply and value creation, which helps strengthen their relationships with customers and pursue more efficient marketing policies and practices, ultimately leading to a competitive advantage. The IoT is expected to evolve into a vast network that includes smart devices and significantly influences people’s behavior, especially decision-making at different stages of the purchasing process.

Purpose The present study aimed to investigate a) the associations between bilateral performance utilizing countermovement jump (CMJ), squat jump (SJ), speed and unilateral CMJ, isokinetic peak torque in knee extension and flexion with angular velocities of 60°/s and 180°/s and tensiomyography (TMG) parameters; b) whether the asymmetries derived from unilateral tests are associated with bilateral CMJ, SJ and speed in elite female soccer players. Methods Thirty-five elite female soccer players (average age: 20 ± 5 years) completed CMJ, SJ, speed, isokinetic muscle strength and TMG tests. Results Compared to the non-dominant leg, the dominant leg demonstrated greater peak torque output in both knee flexion (7.4%) and knee extension (5.6%) isokinetic tasks, as well as m. vastus medialis contraction time (7.6%), and soccer-specific agility test (4.1%). Conversely, the hamstring to quadriceps peak torque ratio at 180°/s (8.5%) was significantly greater in the non-dominant leg. The associations between CMJ, SJ and speed performance were positive and ranged from weak (r = 0.350) to high (r = 0.710). For speed and TMG-derived variables, correlations were negative and ranged from weak (r = −0.345, p = 0.042, for vastus medialis contraction time) to moderate (r = −0.530, p = 0.001, for biceps femoris contraction time). Furthermore, both bilateral CMJ and SJ negatively correlated with TMG-derived variables, ranging from weak (r = −0.350, p = 0.039, for vastus lateralis contraction time) to moderate (r = −0.537, p = 0.003, for rectus femoris contraction time). Conclusion The overall significant, albeit inconsistent, correlations between the diverse performance scores obtained highlight the necessity for a multifaceted and thorough diagnostic strategy in female soccer players.

Background Type 1 diabetes mellitus (T1DM) management in children and adolescents requires intensive supervision and monitoring to prevent acute and late diabetes complications and to improve quality of life. Digital health interventions, in particular diabetes mobile health apps (mHealth apps) can facilitate specialized T1DM care in this population. This study evaluated the initial usability of and satisfaction with the m-Health intervention Diabetes: M app, and the ease of use of various app features in supporting T1DM care in rural and remote areas of Bosnia-Herzegovina with limited access to specialized diabetes care. Methods This cross-sectional study, performed in February–March 2023, evaluated T1DM pediatric patients who used the Diabetes: M app in a 3-month mHealth-based T1DM management program, along with their parents and healthcare providers (HCPs). All participants completed self-administered online questionnaires at the end of the 3-month period. Data were analyzed by descriptive statistics. Results The study population included 50 T1DM patients (children/parents and adolescents) and nine HCPs. The mean ± SD age of the T1DM patients was 14 ± 4.54 years, with 26 (52%) being female. The mean ± SD age of the HCPs was 43.4 ± 7.76 years; all (100%) were women, with a mean ± SD professional experience of 17.8 ± 8.81 years. The app was reported usable in the domains of ease-of-use and satisfaction by the T1DM children/parents (5.82/7.0), T1DM adolescents/young adults (5.68/7.0), and HCPs (5.22/7.0). Various app features, as well as the overall app experience, were rated positively by the participants. Conclusion The results strongly support the usability of mHealth-based interventions in T1DM care, especially in overcoming care shortage and improving diabetes management and communications between HCPs and patients. Further studies are needed to compare the effectiveness of apps used to support T1DM management with routine care.

Background Recent findings point to the key role of cathepsin S (CTSS) in the survival of malignant cells, as well as the significance of the anti-apoptotic properties of high-density lipoprotein (HDL) that contribute to enhanced cell survival. The purpose of this study is to analyse CTSS as a potential biomarker in lymphoma. Also, in order to better understand the role of CTSS in the origin and development of lymphoma, its association with cystatin C (Cys C), lipids, and inflammatory markers was analysed. Methods The study included 90 subjects: 11 Hodgkin (HL) and 44 B-cell non-Hodgkin lymphoma (NHL) patients, as well as 35 healthy subjects. CTSS was determined using the Invitrogen ELISA kit (Thermo Fisher Scientific, Inc., Waltham, MA, USA). The non-denaturing 3%-31% polyacrylamide gradient gel electrophoresis method was used to separate plasma HDL particles. Results The level of CTSS was significantly higher in NHL patients than in control subjects: 12.20 (9.75-14.57) vs 9.97 (8.44-10.99), P<0.001. In NHL patients, there was a positive correlation between CTSS and the proportions of HDL3a, HDL3b, and the sum of the HDL3 subclasses (r=0.506, P<0.001; r=0.411, P=0.006, r=0.335, P=0.026, respectively). In addition, the area under the receiver operating characteristic curve (AUC curve) of CTSS was 0.766 (CI: 0.655-0.856) for NHL patients. There was no significant difference in CTSS values between the control group and patients with HL, nor significant correlations between CTSS and HDL subclasses in the HL group. Conclusions CTSS is significantly elevated in patients with NHL and has the potential to be a new diagnostic bio - marker for the detection of NHL. Also, this study was the first to unveil the association between serum CTSS levels and the proportions of anti-apoptotic HDL3a and HDL3b subclasses in NHL patients.

Background: Recent findings point to the key role of cathepsin S (CTSS) in the survival of malignant cells, as well as the significance of the anti-apoptotic properties of high-density lipoprotein (HDL) that contribute to enhanced cell survival. The purpose of this study is to analyze CTSS as a potential biomarker in lymphoma. Also, in order to better understand the role of CTSS in the origin and development of lymphoma, its association with cystatin C (Cys C), lipids, and inflammatory markers was analyzed.  Methods: The study included 90 subjects: 11 Hodgkin (HL) and 44 B-cell non-Hodgkin lymphoma (NHL) patients, and 35 healthy subjects. CTSS was determined using the Invitrogen ELISA kit (Thermo Fisher Scientific, Inc., Waltham, MA, USA). Results: The level of CTSS was significantly higher in NHL patients than in control subjects: 12.20 (9.75-14.57) vs 9.97 (8.44-10.99), P<0.001. In NHL patients, there was a positive correlation between CTSS and the proportions of HDL3a, HDL3b, and the sum of the HDL3 subclasses (r=0.506, P<0.001; r=0.411, P=0.006, r=0.335, P=0.026, respectively). In addition, the area under the receiver operating characteristic curve (AUC curve) of CTSS was 0.766 (CI: 0.655-0.856). Conclusions CTSS is significantly elevated in patients with NHL and has the potential to be a new diagnostic biomarker. Moreover, demonstrating a correlation between CTSS levels and the proportion of anti-apoptotic HDL3a and HDL3b subclasses improves understanding of NHL, as well as contributes to the development of new therapeutic strategies for this cancer.

Background Among many genes which have been analyzed to understand obesity and related metabolic traits among children and adolescents, not many studies are conducted on LGALS3 gene, especially in population of children. A positive correlation of circulating galectin 3 serum levels with impaired blood glucose, high blood pressure and higher values of serum lipids and was found in general population. The aim was to investigate possible association of rs4644 with body mass index, glycaemia, and lipid profile in Serbian adolescents. Methods The study included 72 boys and 79 girls, 14-15 years of age. Among boys 51 (67.1%) had normal values of BMI, 11 (14.5%) were overweight, and 14 (18.4%) were obese. Among girls, 53 (63.9%) had normal BMI, 16 (19.3%) were overweight, and 14 (16.9%) were obese. Diabetes type 1 or 2, genetic syndromes, generalized inflammation, cardiovascular and malignant diseases were criteria for exclusion. Genotyping was performed by Real time PCR.

This text is a review of a book by Zoran Bibanović Bosnia and Herzegovina. Cultural Gate of Europe, Library National Treasure of Bosnia and Herzegovina, Sarajevo, 2023, presented delivered at the presentation held at the Bosnian Cultural Center in Sarajevo on 29 February 2024.

This article examines the ways in which the media in Bosnia and Herzegovina in times of crisis relate to the already existing social polarization and the ways in which they further strengthen it. Theories of social polarization were used, with a special emphasis on interpretive polarization, in which the media play a crucial role. The focus of the research is reporting on the Ukrainian crisis, in order to consider the patterns that have developed in media reporting, especially when it comes to different approaches in the Federation of Bosnia and Herzegovina and the Republika Srpska. The research is based on the method of content analysis and in-depth structured interviews with journalists, editors and political analysts. The sample consists of the 7 most read web portals from BiH: 4 from the Federation of BiH and 3 from the RS, and the corpus totals 50 texts. A search used the keywords: Ukraine, Russia, aggression, attack, invasion, war, and the analyzed period is seven days from the beginning of the attack on Ukraine (from February 24, 2022) and seven days from the date of the first anniversary of the attack on Ukraine (from February 24 .2023). Indicators for analysis are: how the media decide what is newsworthy, how the media choose sources, how the media verify information and how the media shape the story (with special reference to how they shape it in relation to the agenda they set and the public opinion they shape). The goal is to question how much the Ukrainian crisis was used by the media to further polarize B&H society, and the general conclusion is that media reporting only partially fulfills the key roles of providing comprehensive, clear information, contextualizing events and educating the public. The interpretation of the events is selective and in accordance with the dominant narratives and, as such, does not contribute to a complete understanding of the events, and even less to a high-quality social dialogue. As a post-conflict society, Bosnia and Herzegovina should have a much greater sensitivity to the events in Ukraine, and the media should approach this topic with much more attention, responsibility and public interest. This applies equally to the quantity and quality of information available to citizens.

Gender equality is a democratic and civilizational standard, and mechanisms to implement gender mainstreaming have been established in many countries around the world, including Bosnia and Herzegovina. International mechanisms and women's civil society organizations are the main carriers of these changes, but the real impact of gender mainstreaming depends on the ability of institutions and the commitment of policy makers to move gender from the margins to the center in setting public policy at all levels of government. Unfortunately, across globally, the norm and practice diverge, especially at the local level. In the countries of the Western Balkans, these problems are even more pronounced and insufficiently articulated in academic research. Therefore, this paper analyzes the results of a survey conducted in local communities in Bosnia and Herzegovina. The research included an analysis of legal provisions at the local level and interviews with relevant stakeholders (women activists and members of local equality commissions). The analysis shows that gender equality is addressed in a fragmented and inconsistent manner, that it is not adequately addressed in local documents, and that local gender mechanisms (commissions for gender equality) have been formed to fulfill a formal obligation. In communities with no profiled women's organizations, the situation is even worse when it comes to the possibility of monitoring and improving the work of these mechanisms.

Ageing is a complex biological process with variations among individuals, leading to the development of ageing clocks to estimate biological age. Glycans, particularly in immunoglobulin G (IgG), have emerged as potential biomarkers of ageing, with changes in glycosylation patterns correlating with chronological age. For precision analysis, three different plasma pools were analysed over 26 days in tetraplicates, 312 samples in total. In short-term variability analysis, two cohorts were analysed: AstraZeneca MFO cohort of 26 healthy individuals (median age 20) and a cohort of 70 premenopausal Chinese women (median age 22.5) cohort monitored over 3 months. Long-term variability analysis involved two adult men aged 47 and 57, monitored for 5 and 10 years, respectively. Samples were collected every 3 months and 3 weeks, respectively. IgG N-glycan analysis followed a standardized approach by isolating IgG, its subsequent denaturation and deglycosylation followed by glycan cleanup and labelling. Capillary gel electrophoresis with laser-induced fluorescence (CGE-LIF) and ultra-performance liquid chromatography analyses were employed for glycan profiling. Statistical analysis involved normalization, batch correction, and linear mixed models to assess time effects on derived glycan traits. The intermediate precision results consistently exhibited very low coefficient of variation values across all three test samples. This consistent pattern underscores the high level of precision inherent in the CGE method for analysing the glycan clock of ageing. The AstraZeneca MFO cohort did not show any statistically significant trends, whereas the menstrual cycle cohort exhibited statistically significant trends in digalactosylated (G2), agalactosylated (G0) and fucosylation (F). These trends were attributed to the effects of the menstrual cycle. Long-term stability analysis identified enduring age-related trends in both subjects, showing a positive time effect in G0 and bisected N-acetylglucosamine, as well as a negative time effect in G2 and sialylation, aligning with earlier findings. Time effects measured for monogalactosylation, and F remained substantially lower than ones observed for other traits. The study found that IgG N-glycome analysis using CGE-LIF exhibited remarkably high intermediate precision. Moreover, the study highlights the short- and long-term stability of IgG glycome composition, coupled with a notable capacity to adapt and respond to physiological changes and environmental influences such as hormonal changes, disease, and interventions. The discoveries from this study propel personalized medicine forward by deepening our understanding of how IgG glycome relates to age-related health concerns. This study underscores the reliability of glycans as a biomarker for tracking age-related changes and individual health paths.