The objective of this paper is to evaluate and distinguish the most critical segments of the residential building stock in Bosnia and Herzegovina in terms of its energy performance, in order to define the guidelines and priorities in the development of the energy efficiency strategies and building refurbishment plans. The research methodology will be explained systematic in the case of the most dominant typology in Bosnia and Herzegovina: the single-family houses built from 1981 to 1991. The analysis will include an overview of the average features for the selected category, which were obtained from the statistical survey and embraces several aspects: urbanism, architecture building physics and heating system. Subsequent methodological steps included the selection of the “representative” or the “typical” buildings, which are characterized by average or the most typical properties of each category within the entire database. This paper will present the analysis, which intend to demonstrate that, according to their potential in terms of possible energy savings, based on the presented improvement measures, the highlighted building typology – the single-family houses of 27 to 37 years of age – can be considered as the most relevant building typology in developing the strategies and plans of the residential building stock refurbishment. This paper is a part of the research on the typology of existing residential buildings in Bosnia and Herzegovina, which was initiated in 2014. Being the first-of-a-kind systematic approach on the subject matter, this research intends to catalyse the shift to the energy efficient housing models in a post-transition and post-conflict country such as Bosnia and Herzegovina.

Building industry produces 38% of total waste and 40% of total CO2 emissions and uses 50% of all natural resources (EIB 2015). Taking this into account, decreasing the waste from any part of the building will help reducing total waste during the building life span. In this paper we will showcase the indicators for measuring of the waste creation from building façade upon transformation. Façade should follow transformations of the building without waste creation or without causing large financial or environmental impacts. The planning of building industry waste management begins in the design phase, as the waste prevention is the preferred option, and reuse, recycling, and other types of recovery are a second option according to EC. (Directive 2008/98/EC 2008). This paper will show the comparison between the conventional façade system made of wood and the prototype of GDC (Green Design Center) reversible façade system addressing the waste elimination. The reversible façade has been designed using design protocol for designing of low waste façade system for the future circular construction industry developed by Durmisevic 2017 as part of EU BAMB project. The protocol contains a rulebook with tools to measure the waste production during the transformation of building façade. This strategy aims to extend functional lifespan of external envelope of buildings and its components and materials, which would reduce the amount of consumed resources and generated waste during the lifespan of the façade. The contribution of this paper will also be in the field of connections in the building industry, as the importance of type of connections in the façade system with high reuse potential is noticeable.

This research focuses on analyzing residents' perception and attitude toward tourism development in the Federation of Bosnia and Herzegovina (FB&H). For the purpose of this research, the existing models on residents' perception and attitudes were modified and a new theoretical model of six constructs was tested applying Confirmatory Factor Analysis (CFA). The dimensions of the model, perception of tourism management, perception of economic, social and environmental impacts, residents' satisfaction, support for further tourism development, were formed as latent variables, accompanied with a set of three or four consonant questions. The model suggests that the support for tourism development is indirectly stipulated by the perception of tourism management, and by economic, social and environmental impacts. Analyses have shown that 6 out of the 7 suggested hypotheses have been confirmed. The findings indicate that the residents of the FB&H strongly support tourism development as they perceive it brings more positive impacts, which are greater than the actual governmental investment into the sector.

The architecture initiated during the time of the Austro-Hungarian period was distinctly expressed both in Sarajevo and in the whole region of Bosnia-Herzegovina. A substantial number of these buildings was assessed for their historical, architectural, ambient and aesthetical values and, as such, represent the valuable historic heritage of these regions. Standing among them in its impressive dimension and being of a great national value is the palace of the former Provincial Government, which was designed by Josip Vancaš in Vienna (1884). The aim of the present paper is to emphasize the importance of atrial and open spaces integrated into the volume of the building. These spaces can be used to synthesize and functionally transform spaces into controlled and contextually selected forms, contemporary shapes and materials by applying functional creativity without endangering the ambience and historical values of the building. On the contrary, by closing the courtyard and atrial spaces, it would be possible to ensure a buffer zone between the interior and the exterior as well as to create a micro-climate and eliminate processes that generally occur in façades facing such spaces. These spaces could be synthesized and functionally transformed into useable, controlled and contextually selected forms by creatively applying contemporary shapes and materials without endangering the ambiance and historic values of the building. The view towards the sky through a diagonal lattice would present an association to the “lattice window” or “mušebak”, creating a comfortable microclimate. Several different structural concepts were studied. The aim of the paper was to evaluate structural concepts in terms of feasibility and energy efficiency measures pay off. There are numerous examples of transparent roof extensions introduced when rehabilitating the existing cultural-historical buildings, the extensions known for their power in terms of quality and quantity or the harmonious bond between the old and the new. The atrium roof structure will be supported on the walls of existing building. Since the roof structure would be on the interior of the building footprint it is important to consider manageable erection process.

Introduction The system of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (cas) is a new technology that allows easier manipulation of the genome. Its potential to edit genes opened a new door in treatment development for incurable neurological monogenic diseases (NMGDs). The aim of this systematic review was to summarise the findings on the current development of CRISPR-cas for therapeutic purposes in the most frequent NMGDs and provide critical assessment. Methods and data acquisition We searched the MEDLINE and EMBASE databases, looking for original studies on the use of CRISPR-cas to edit pathogenic variants in models of the most frequent NMGDs, until end of 2017. We included all the studies that met the following criteria: 1. Peer-reviewed study report with explicitly described experimental designs; 2. In vitro, ex vivo, or in vivo study using human or other animal biological systems (including cells, tissues, organs, organisms); 3. focusing on CRISPR as the gene-editing method of choice; and 5. featured at least one NMGD. Results We obtained 404 papers from MEDLINE and 513 from EMBASE. After removing the duplicates, we screened 490 papers by title and abstract and assessed them for eligibility. After reading 50 full-text papers, we finally selected 42 for the review. Discussion Here we give a systematic summary on the preclinical development of CRISPR-cas for therapeutic purposes in NMGDs. Furthermore, we address the clinical interpretability of the findings, giving a comprehensive overview of the current state of the art. Duchenne’s muscular dystrophy (DMD) paves the way forward, with 26 out of 42 studies reporting different strategies on DMD gene editing in different models of the disease. Most of the strategies aimed for permanent exon skipping by deletion with CRISPR-cas. Successful silencing of the mHTT gene with CRISPR-cas led to successful reversal of the neurotoxic effects in the striatum of mouse models of Huntington’s disease. Many other strategies have been explored, including epigenetic regulation of gene expression, in cellular and animal models of: myotonic dystrophy, Fraxile X syndrome, ataxias, and other less frequent dystrophies. Still, before even considering the clinical application of CRISPR-cas, three major bottlenecks need to be addressed: efficacy, safety, and delivery of the systems. This requires a collaborative approach in the research community, while having ethical considerations in mind.

We examined the associations between germline variants and breast cancer mortality using a large meta-analysis of women of European ancestry. Meta-analyses included summary estimates based on Cox models of twelve datasets using ~10.4 million variants for 96,661 women with breast cancer and 7697 events (breast cancer-specific deaths). Oestrogen receptor (ER)-specific analyses were based on 64,171 ER-positive (4116) and 16,172 ER-negative (2125) patients. We evaluated the probability of a signal to be a true positive using the Bayesian false discovery probability (BFDP). We did not find any variant associated with breast cancer-specific mortality at P < 5 × 10−8. For ER-positive disease, the most significantly associated variant was chr7:rs4717568 (BFDP = 7%, P = 1.28 × 10−7, hazard ratio [HR] = 0.88, 95% confidence interval [CI] = 0.84–0.92); the closest gene is AUTS2. For ER-negative disease, the most significant variant was chr7:rs67918676 (BFDP = 11%, P = 1.38 × 10−7, HR = 1.27, 95% CI = 1.16–1.39); located within a long intergenic non-coding RNA gene (AC004009.3), close to the HOXA gene cluster. We uncovered germline variants on chromosome 7 at BFDP < 15% close to genes for which there is biological evidence related to breast cancer outcome. However, the paucity of variants associated with mortality at genome-wide significance underpins the challenge in providing genetic-based individualised prognostic information for breast cancer patients.

In 2012, alcohol liver disease resulted in 3.3 million—5.9% of global deaths. This study introduced whey protection capacity against chronic alcohol-induced liver injury. Rats were orally administered to 12% ethanol solution in water (ad libitum, average 8.14 g of ethanol/kg body weight (b.w.)/day) alone or combined with whey (per os, 2 g/kg b.w./day). After 6-week treatment, chronic ethanol consumption induced significant histopathological liver changes: congestion, central vein dilation, hepatic portal vein branch dilation, Kupffer cells hyperplasia, fatty liver changes, and hepatocytes focal necrosis. Ethanol significantly increased liver catalase activity and glutathione reductase protein expression without significant effects on antioxidative enzymes: glutathione peroxidase (GPx), copper–zinc-containing superoxide dismutase (CuZnSOD) and manganese-containing superoxide dismutase (MnSOD). Co-treatment with whey significantly attenuated pathohistological changes induced by ethanol ingestion and increased GSH-Px and nuclear factor kappa B (NF-κB) protein expression. Our results showed positive effects of whey on liver chronically exposed to ethanol, which seem to be associated with NF-κB-GPx signaling.

In this paper, we consider the topic from the theory of cosine operator functions in 2-dimensional real vector space, which is an interplay between functional analysis and matrix theory. For the various cases of a given real matrix A= [α , β; γ , δ] we find out the appropriate cosine operator function C(t)= [a(t), b(t); c(t), d(t)], (t \in R) in a real vector space R2 as the solutions of the Cauchy problem C''(t)=AC(t), C(0)=I, C'(0)=0.