Aim Any aesthetic procedure in the head and/or in the face might have an impact on psychological status of the treated participants. Aim of this study was to investigate whether Botulinum toxin treatment for aesthetic purpose in the face influences on the level of happiness, depression and anxiety. Methods This prospective cohort observational study included 30 participants, who were treated by botulinum toxin (Botox) due to aesthetic corrections. The treatment included laugh lines, frown lines and horizontal forehead lines. Preprocedural, three and six months after the treatment the participants were assessed by The Oxford Happiness Questionnaire (OHQ), The Beck Depression Inventory (BDI) and The Beck Anxiety Inventory (BAI) to determine the level of happiness, anxiety and depression was used. Results Three months after the treatment by Botox the level of happiness was significantly increased (5.26±0.43 vs 4.3±0.34; p<0.0001). The levels of depression (7.6±6.0 vs 14.2±8.3; p<0.0001) and anxiety (8.8±6.3 vs 16.4±8.8; p<0.0001) were significantly decreased compared with preprocedural level. Significant increased level of happiness and decreased levels of depression and anxiety remained six months after the treatment, but attenuated. A dose of applied botulinum toxin was negatively correlated with the level of depression (r = -0.394; p=0.0421) and anxiety (r = -0.387; p=0.0302). Conclusion Botulinum toxin treatment for aesthetic purpose in the face positively influences psychological status of the treated individual in the short-therm.

Aim This study compared the extent of coronary artery calcification in patients with and without type 2 diabetes mellitus (T2DM) using Coronary Computed Tomography Angiography (CCTA). Methods This retrospective, observational cohort study included 107 patients who underwent CCTA at the Clinical Centre of the University of Sarajevo between July and December 2024. Patients were divided into two groups: those with T2DM (n=51) and those without T2DM (n=56). Laboratory parameters, demographic data, and calcium scores were analysed. The calcium score was categorised into six groups based on cardiovascular risk and the comparison was made using appropriate statistical analysis. Results Patients with T2DM had significantly higher calcium scores than non-diabetic patients (p=0.0001). In the T2DM group, 35.3% of patients had a calcium score >400, indicating high cardiovascular risk. Patients without diabetes were more frequently classified into lower-risk categories (p=0.0001). A significant correlation was found between calcium score and age (r=0.442, p=0.001) and gender (r=-0.218, p=0.024), with men having higher calcium scores. Additionally, total cholesterol, LDL, and uric acid levels were significantly higher in diabetic patients (p=0.005; p=0.025; p=0.03, respectively). Conclusion This study confirms a strong association between T2DM and increased coronary artery calcification. Age and male gender are significant predictors of higher calcium scores. Further research is needed to explore these relationships, particularly within the Bosnian population. Keywords Coronary angiography, coronary artery calcification, coronary disease, diabetes mellitus type 2.

Differentiated or HPV‐independent vulvar intraepithelial neoplasia (dVIN) can progress rapidly to invasive cancer and accurate pathological diagnosis is essential to facilitate appropriate interventions. Histological similarities of dVIN with non‐neoplastic lesions, however, often make the diagnosis less reproducible. We investigated among a diverse group of pathologists whether the diagnostic agreement improves with the use of p53 immunohistochemistry (IHC) interpreted using the pattern‐based schema.

Aim To investigate the relationship between postoperative serum thyroglobulin level ​​and outcome of therapy with I-131 of follicular thyroid cancer. Methods A total of 106 patients with follicular thyroid cancer who were being treated and monitored at the Clinic for Nuclear Medicine at Clinical Center of the University of Sarajevo were included. The inclusion criteria were: surgery of total thyroidectomy, histopathological diagnosis of follicular thyroid cancer, and applied therapy with radioactive iodine. Exclusion criteria were patients with incomplete data, who were not treated with radioidine treatment, or had a different histopathological diagnosis. Postoperative serum thyroglobulin levels were correlated with results of whole body scintigraphies after 12 months (first diagnostic scintigraphy) and whole body scintigraphies after 24 months (second diagnostic scintigraphy). Results The higher frequency of recurrent disease in patients with elevated level of thyroglobulin was found compared to patients with lower postoperative thyroglobulin level. Elevated level ​​of postoperative thyroglobulin correlated with positive scintigraphy findings, i.e., with the occurrence of recurrence and/or metastases in patients with follicular cancer. The cut-off level of postoperative thyroglobulin for recurrence and/or metastasis, i.e. for failure of ablative therapy with I-131, was >12.6 ng/mL. Conclusion  Our study showed that level of postoperative thyroglobulin is an important prognostic factor for the outcome of radiodine therapy of follicular thyroid cancer and should be taken into account in deciding on therapy in this type of cancer in everyday practice Keywords: prognosis, radioiodine, thyroid neoplasm, recurrence.

Spondylosis deformans (SD) is a common form of degenerative spinal disease in dogs, characterized byosseous bridging between vertebral bodies. This study focused on the lumbar and lumbosacral regions ofthe spine and analyzed radiographic records of 35 dogs of various breeds and age groups over a 12-monthperiod. Computed tomography (CT) was selectively employed in cases where radiographic interpretationwas inconclusive. The objective was to determine lesion localization and assess associations with breed, age,and gender. Findings showed that German Shepherds were most frequently diagnosed with SD, followedby Labrador Retrievers and mixed-breed dogs. Age distribution indicated that no cases were observed indogs aged 1-2 years, while the highest prevalence occurred in the 7-10-year age group. Lesion localizationdata revealed that the L4-L6 region was most commonly affected. These results highlight the age-relatedprogression of SD and its predilection for the lumbar spine, offering valuable insights for clinical assessmentand management in veterinary practice.

This study focuses on evaluating the antioxidant activity of pomegranate (lat. Punica granatum L.) peel extracts obtained through various extraction methods, including ultrasound-assisted extraction (UAE), Soxhlet extraction, and maceration, using methanol and 96% ethanol as solvents. These techniques were chosen for their differing effects on the preservation of thermosensitive phytochemicals. Antioxidant potential was assessed using the DPPH radical scavenging assay, with absorbance measured at 517 nm using a UV-Vis spectrophotometer. The results demonstrated that the efficiency of antioxidant activity largely depended on the extraction method and solvent used. A lower IC50 value indicates higher antioxidant activity, as it reflects a greater efficiency in neutralizing free radicals at a lower concentration. Extracts obtained by ultrasound-assisted extraction exhibited the highest radical scavenging capacity with an IC50 value of 19.049 μg/mL, while those obtained by Soxhlet extraction with ethanol showed comparatively weaker activity with an IC50 value of 34.210 μg/mL, likely due to the thermal degradation of sensitive bioactive compounds. The maceration method, although mild and solvent-efficient, yielded moderate antioxidant activity, highlighting the balance between extraction intensity and preservation of functional constituents. The study emphasizes the importance of optimizing extraction conditions to maximize the recovery of bioactive compounds from plant materials. Given the phytochemical richness and biological potential of pomegranate peel, the findings support its application as a natural source of antioxidants in the development of dermocosmetic and pharmaceutical formulations aimed at combating oxidative stress and disorders related to hyperpigmentation and skin aging.

Background It has long been hypothesized that increasing heritability with age of cognitive and educational performance is partly attributable to evocative gene–environment correlation. However, this hypothesis has not been widely tested. Methods We addressed this gap by examining whether children's education polygenic scores (PGSedu ) were associated with maternal self‐reported positive and literacy‐focused parenting when children were 5 years old, and if evoked parenting differences mediated genetic effects on children's educational outcomes (mother‐reported at 6–8 years of age), while controlling for parental PGSedu . We also investigated whether maternal reports of children's language at 5 years old were associated with parenting and mediated genetic effects on educational performance. These questions were addressed in a sample of 83,627 parent‐offspring trios from the Norwegian Mother, Father and Child Cohort Study, a longitudinal population‐based pregnancy cohort. Results Children's PGSedu were significantly associated with maternal literacy‐focused (β = .03, 95% CI [0.01, 0.05], p = .021) but not positive parenting (β = 0.01, 95% CI [−0.02, 0.05], p = .410), and literacy‐focused parenting significantly mediated the effects of children's PGSedu on their educational performance (β = 0.01, 95% CI [1 × 10−3, 0.01], p = .023). Children's language was associated with maternal parenting and mediated the effects of children's PGSedu on their educational performance (β = 0.01, 95% CI [3 × 10−3, 0.02], p = .002). Conclusions These findings support our hypotheses and suggest early language and parenting may be mechanisms implicated in the pathways from children's genetics to their educational outcomes.

Personalised nutrition (PN) has emerged as an approach to optimise individual health outcomes through more targeted and tailored dietary recommendations based on unique genetic, phenotypic, medical, lifestyle and contextual factors. The application of artificial intelligence (AI) presents an opportunity to achieve personalised nutrition advice at a scale that has population impact. This review introduces a nutrition audience to different AI applications and offers insights into the concepts of AI that might be relevant to the field of nutrition research. The current and future uses of AI in PN are discussed, as well as the potential benefits and challenges to their application. AI-driven solutions have the potential to improve health and reduce the risk of disease because they can consider more information about an individual in making recommendations. However, challenges such as data interoperability, ethical considerations, and model interpretability remain an issue limiting widespread use at this point. This review will provide a foundational understanding of the application of AI within PN and help to identify opportunities to leverage the potential of AI in transforming dietary guidance and enhancing health outcomes through innovative solutions.

Plant species with strong antioxidant activity used in traditional medicine of B&H-Sambucus nigra, Filipendula vulgaris, Helichrysum italicum, Epilobium angustifolium, Crataegus rhipidophylla, Thymus serpyllum, Vaccinium myrtillus, Symphytum officinale, Corylus avellana, and Rubus fruticosus-were analysed for their phenolic profiles and cholinesterase inhibitory activity. The HPLC-DAD analysis revealed the highest concentration of phenolic acids in S. officinale extract. Catechin, rutin, and quercetin were identified in the majority of extracts. Rutin was most abundant, especially in S. nigra flowers (9.39 mg/g DW). AChE and BChE inhibition was determined spectrophotometrically. All extracts showed activity, with AChE IC50 ranging from 0.08 mg/mL (V. myrtillus) to 8.31 mg/mL (H. italicum), and BChE from 5.35 mg/mL (T. serpyllum) to 13.26 mg/mL (C. rhipidophylla). These findings highlight the neuroprotective potential of B&H medicinal plants, with molecular docking showing phenolics like rosmarinic acid and rutin inhibit cholinesterases. Merging traditional medicinal knowledge and molecular insights offers a novel path for discovery.

This study aims to investigate individual and joint effects of digitalization and inbound open innovation (OI) on technological (product and process) innovations in German firms. In particular, two specific forms of digitalization are considered – artificial intelligence (AI) and big data analytics (BDA), as well as the search breadth as a measure of inbound OI. To answer this research question, an econometric analysis is conducted on a sample of Germany firms using the Mannheim Innovation Panel dataset from the wave conducted in 2019 and covering the period 2016–2018. Moreover, the study reports results for the manufacturing and service sectors separately. This sectoral analysis sheds light on any potential differences in the innovation effects of digitalization and OI practices. Our empirical findings are heterogeneous and show that, overall, AI positively impacts product innovation, while BDA increases the likelihood of process innovation. Moreover, AI and the breadth of knowledge search do not yield synergistic innovation effects, while BDA weakens the positive impact of search breadth on process innovation. Based on empirical findings, we discuss theoretical, managerial and policy implications. This study contributes to the literature by examining the influence of digitalization and inbound OI on technological, product and process innovations. In addition, this study examines the mediating effect that digitalization and inbound OI can exert on technological innovation in German firms. The full sample is divided into manufacturing and service sectors to disentangle potentially heterogeneous effects of inbound OI and digitalization on innovation performance.

Previous studies have demonstrated the feasibility of estimating the speech reception threshold (SRT) based on electroencephalography (EEG), termed SRTneuro, in younger normal-hearing (YNH) participants. This method may support speech perception in hearing-aid users through continuous adaptation of noise-reduction algorithms. The prevalence of hearing impairment and thereby hearing-aid use increases with age. The SRTneuro estimation is based on envelope reconstruction accuracy, which has also been shown to increase with age, possibly due to excitatory/inhibitory imbalance or recruitment of additional cortical regions. This could affect the estimated SRTneuro. This study investigated the age-related changes in the temporal response function (TRF) and the feasibility of SRTneuro estimation across age. Twenty YNH and 22 older normal-hearing (ONH) participants listened to audiobook excerpts at various signal-to-noise ratios (SNRs) while EEG was recorded using 66 scalp electrodes and 12 in-ear-EEG electrodes. A linear decoder reconstructed the speech envelope, and the Pearson's correlation was calculated between the reconstructed and speech-stimulus envelopes. A sigmoid function was fitted to the reconstruction-accuracy-versus-SNR data points, and the midpoint was used as the estimated SRTneuro. The results show that the SRTneuro can be estimated with similar precision in both age groups, whether using all scalp electrodes or only those in and around the ear. This consistency across age groups was observed despite physiological differences, with the ONH participants showing higher reconstruction accuracies and greater TRF amplitudes. Overall, these findings demonstrate the robustness of the SRTneuro method in older individuals and highlight its potential for applications in age-related hearing loss and hearing-aid technology.

Background: Comorbid personality disorders (PDs) in patients with anorexia nervosa (AN) are associated with increased psychopathology, higher suicide risk, and poorer treatment response and outcomes. This study aimed to examine associations between gray matter (GM) volume and PDs in female adolescents with AN before and after short-term psychotherapeutic and nutritional therapy. Methods: Eighteen female adolescents with acute AN, mean age 15.9 years, underwent 3T magnetic resonance imaging before and after weight restoration. The average interval between scans was 2.6 months. Structural brain changes were analyzed using voxel-based morphometry. PDs were assessed using the Structured Clinical Interview for DSM-IV Axis II Disorders (SCID II) and the Assessment of Identity Development Questionnaire. Results: SCID-II total scores showed significant positive associations with GM volume in the mid-cingulate cortex at both time points and in the left superior parietal–occipital lobule at baseline. The histrionic subscale correlated with GM volume in the thalamus bilaterally and the left superior parietal–occipital lobule in both assessments, as well as with the mid-cingulate cortex at follow-up. Borderline and antisocial subscales were associated with GM volume in the thalamus bilaterally at baseline and in the right mid-cingulate cortex at follow-up. Conclusions: PDs in female adolescent patients with AN may be specifically related to GM alterations in the thalamus, cingulate, and parieto-occipital regions, which are present during acute illness and persist after weight restoration therapy.