Within the immune system, microRNAs (miRNAs) exert key regulatory functions. However, what are the mRNA targets regulated by miRNAs and how miRNAs are transcriptionally regulated themselves remain for the most part unknown. We found that in primary human memory T helper lymphocytes, miR-150 was the most abundantly expressed miRNA, and its expression decreased drastically upon activation, suggesting regulatory roles. Constitutive MIR150 gene expression required the RFX family of transcription factors, and its activation-induced down-regulation was linked to their reduced expression. By performing miRNA pull-down and sequencing experiments, we identified PDGFA-associated protein 1 (PDAP1) as one main target of miR-150 in human T lymphocytes. PDAP1 acted as an RNA-binding protein (RBP), and its CRISPR/Cas-9–mediated deletion revealed that it prominently contributed to the regulation of T-cell proliferation. Overall, using an integrated approach involving quantitative analysis, unbiased genomics, and genome editing, we identified RFX factors, miR-150, and the PDAP1 RBP as the components of a regulatory axis that restrains proliferation of primary human T lymphocytes.

In the scope of the genetic, morphological and phenological research of the genus Tilia in Bosnia and Herzegovina, with control populations in Germany, multibracteate silver-leaved linden (T. tomentosa Moench.) was discovered in Mostar. In addition to the usual primary bract, there are also bracts of the second and the third order, which as phenomenon is, to our knowledge, new for the science. Since the bracts vary in size, as well as in shape, and additional bracts do not appear on all the sampled trees, the task in the future is to further investigate this phenomenon.

Abstract Purpose: The purpose of this article is to examine the relationship between employee participation in the decision-making process (EPDMP) and organizational citizenship behavior (OCB) while considering the mediating role of affective commitment (AC). Methods: The article is based on primary cross-sectional data collected using questionnaires and applying a convenience sampling method among employees in Bosnia and Herzegovina (B&H). The sample consists of 302 employees from 127 companies across multiple industries. Results: First, there is a positive relationship between EPDMP and two dimensions of OCB – organizational citizenship behavior directed at individuals (OCBI) and organizational citizenship behavior directed at the organization (OCBO). Second, the findings suggest that AC mediates the relationship between EPDMP and OCBI and OCBO. Furthermore, OCBI is found to mediate the relationship between AC and OCBO. Conclusion: This article extends the literature by introducing the mediating role of AC in the relationship between EPDM and two dimensions of OCB and the mediating role of OCBI in the relationship between AC and OCBO.

Motivated by the recent paper [M.R.S. Kulenović, M. Nurkanović, and A.A. Yakubu, Asymptotic behaviour of a discrete-time density-dependent SI epidemic model with constant recruitment, J. Appl. Math. Comput. 67 (2021), pp. 733–753. DOI:10.1007/s12190-021-01503-2], in this paper, we consider the class of the SI epidemic models with recruitment where the Poisson function, a decreasing exponential function of the population of infectious individuals, is replaced by a general probability function that satisfies certain conditions. We compute the basic reproduction number We establish the global asymptotic stability of the disease-free equilibrium (GAS) for We use the Lyapunov function method developed in [P. van den Driessche and A.-A. Yakubu, Disease extinction versus persistence in discrete-time epidemic models, Bull. Math. Biol. 81 (2019), pp. 4412–4446], to demonstrate the GAS of the disease-free equilibrium and uniform persistence of the considered class of models. We show that the considered type of model is permanent for . For the transcritical bifurcation appears. For we prove the global attractivity result for endemic equilibrium and instability of the disease-free equilibrium. We apply theoretical results to specific escape functions of the susceptibles from infectious individuals. For each case, we compute the basic reproduction number .

Aim To examine the prevalence of depression in patients after acute myocardial infarction (AMI), as well as the relationship between the depression and quality of life. Methods The survey was conducted via sociodemographic questionnaire, Beck Depression Inventory (BDI), and Short Form 36 Health Survey questionnaire (SF-36). The result of SF-36 is expressed in subscales that make up the health status profile, i.e. physical functioning, physical role, emotional role, social functioning, mental health, vitality, pain and general health. Results The study included 120 patients, of which 70 males and 50 females aged between 41 and 88 years (mean 64.73±11.218). All patients were hospitalized at the Clinical Centre of the University of Sarajevo, Clinic for Cardiovascular Disease and Rheumatism, due to complications caused by AMI. After AMI 59 (49.17%) patients had depression. Depression was negatively associated with physical functioning, physical role, emotional role, social functioning, mental health, vitality, pain and general health. Physical functioning (r= -0.701; p<0.01) and physical role (r = -0.538; p<0.01) had the highest correlation with depression. Conclusion The evaluation of depressive symptoms after AMI is imperative, because the appearance of symptoms could have an effect on the patient's quality of life.

This study was to examine the response of biofertilizer formulation with the addition of consortium of microbes and biosurfactant DEA palm olein to the growth of cacao seedling. This research used randomized block design in factorial with two factors. The first factor was a biofertilizer formulation with addition of consortium of microbes consisting of seven levels: control; 2.5x107; 5x107; 7.5x107; 10x107; 12.5x107; and 15x107 CFU/mL. The second factor was the addition of biosurfactant DEA palm olein consisting of two levels, which are with and without the biosurfactant DEA palm olein. The result showed that the biofertilizer formulation with the addition of consortium of microbes was significantly affected the cacao height on 4 WAT (week after treatment), the leaf number on 6 and 12 WAT, and also in its root fresh weight parameters. The best level of the first factor was 5x107 CFU/mL. The respond of cacao height on 12 WAT and cacao root dry weight was significant to the addition of biosurfactant DEA palm olein. The best level of the second factor was with the addition of biosurfactant DEA palm olein.

A clear margin is an important prognostic factor for most solid tumours treated by surgery. Intraoperative fluorescence imaging using exogenous tumour-specific fluorescent agents has shown particular benefit in improving complete resection of tumour tissue. However, signal processing for fluorescence imaging is complex, and fluorescence signal intensity does not always perfectly correlate with tumour location. Raman spectroscopy has the capacity to accurately differentiate between malignant and healthy tissue based on their molecular composition. In Raman spectroscopy, specificity is uniquely high, but signal intensity is weak and Raman measurements are mainly performed in a point-wise manner on microscopic tissue volumes, making whole-field assessment temporally unfeasible. In this review, we describe the state-of-the-art of both optical techniques, paying special attention to the combined intraoperative application of fluorescence imaging and Raman spectroscopy in current clinical research. We demonstrate how these techniques are complementary and address the technical challenges that have traditionally led them to be considered mutually exclusive for clinical implementation. Finally, we present a novel strategy that exploits the optimal characteristics of both modalities to facilitate resection with clear surgical margins.