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Anamarija Matijević, Ajla Šakonjić, S. Murtić

Stress caused by drought induces plant morphology, biochemistry, and physiology changes, leading to considerable reductions in plant growth and productivity. This study aimed to evaluate the antioxidant defence system of impatiens seedlings (Impatiens walleriana L.) under drought. The antioxidant response of impatiens to drought was evaluated using following parameters: the activity of catalase, guaiacol peroxidase, pyrogallol peroxidase and ascorbate peroxidase, total phenolic and flavonoids contents and total antioxidant capacity. The experiment was conducted during 2020 in a greenhouse under controlled conditions. Half of the impatiens seedlings (20 plants), after the acclimation period in the greenhouse, were exposed to drought for a period of five days, while the second half was not (20 plants were regularly watered). The results of the study showed that the exposure of impatiens seedlings to drought increased the activity of enzymatic components, total phenolics and flavonoids contents and total antioxidant capacity of leaves. Greater exposure of impatiens to drought (in the observed period) implied a higher plant enzymatic and non-enzymatic antioxidant defence system activity. These results confirm that impatiens has evolved both enzymatic and non-enzymatic antioxidant defence mechanisms to adapt and survive the short-term drought exposure.

Public-Private Partnership (PPP) is an organizational model of public service delivery in which a public partner joins with a private one for a joint delivery of a public service, creating added value for both parties in this “partnership”. From the point of view of the public partner, it decreases public expenditure, increases the quality and distribution of public services, and reduces agency costs. From the point of view of the private partner, the benefits are in achieving the planned rate of return, reducing sub-investment costs, reducing agency costs, and avoiding debt risk. There are numerous studies on the correlation between economic development and investment through PPP, which have led to this research. According to the legal framework in Bosnia and Herzegovina, which is composed of entity and cantonal laws, a subject of PPPs may also be construction, use, maintenance and management or reconstruction of property for the purposes of meeting public needs in the health service sector. The aim of the paper is to explore the legal and institutional assumptions of investing through the PPP model in Bosnia and Herzegovina health service sector and to make recommendations for improving the PPP situation in the health service sector. In addition to the introduction and conclusion, the paper is structured in three parts. The first part represents a concise theoretical framework in which the subject of the research is situated, the second part presents the state of affairs pertaining to investments in the health service sector, and the third part analyzes the current state of the use of this form of public service delivery in Bosnia and Herzegovina. The concluding section makes recommendations for improvements in terms of improving the investment through PPP in the sector of health care services.

V. Rebić, M. Aljičević, V. Landeka, Džanela Mešinović, Amina Karić

Cottage cheese is the largest segment of the dairy market and is most often consumed as a fresh food. The microbiological quality of domestic cottage cheese can pose a problem for public health. Cottage cheese belongs to a group of foods having a potential public health risk. The aim of this study was to conduct microbiological research to determine the level of sanitary safety of cottage cheese types acquired from the most frequents markets of Sarajevo Canton (Bosnia and Herzegovina). Of the total (n=40) analysed cheese types, 22 samples (55%) proved to be meet sanitary requirements, while 18 samples (45%) did not meet microbiological quality, pursuant to the microbiological criteria for cheese stipulated by the National Regulation of Microbiological Criteria B&H and Guidelines for Microbiological Food Criteria B&H. The study included 24 samples of pasteurised and 16 samples of unpasteurised milk. However, five cheese samples (20.8%) from pasteurised milk, and 13 samples (81.3%) from unpasteurised milk were non-compliant. Microbiological analysis was conducted for compulsory and several recommended microorganisms: Salmonella spp., Listeria monocytogenes, coagulase positive staphylococci and other species (Staphylococcus aureus), Escherichia coli, and yeasts and moulds. Our findings confirm that the most common bacteria were S. aureus and E. coli. Also, yeasts and moulds were detected but were within the permissible concentrations. Salmonella spp. and L. monocytogenes were not detected in any of the examined samples.

C. Costa, C. Coração

O presente trabalho investiga como os elementos discursivos e os estético-políticos da cypher Favela Vive, e de certa tradição do rap nacional, representam, na presença do território, a nostalgia e a melancolia. Para isso, mobilizamos um olhar decolonial sobre o contexto de sociabilidade dos artistas e sua relação com a cultura midiática, propondo uma maneira particular de abordar ambos os sentimentos. Nesse sentido, notamos que, apesar de o saldo do espírito nostálgico ser a melancolia, a potencialidade desse movimento está na reelaboração estético-política do presente e do passado, que deixa entrever a redenção ou o porvir.

Samela Selimović, S. Huseinović, J. Kamberović, Sanida Bektić, A. Đozić

Benthic diatoms have gained prominence as environmental bioindicators since the beginning of the twentieth century. Epilithic diatom taxa are frequently used for ecological water-quality assessments of rivers. Diversity of these dominant group of protists in the benthic communities can be indicative of changes in the freshwater ecosystem. The aim of this study was to analyze the diversity and ecology of diatoms of the Spreča River on the locations affected by urban and industrial pollution. Field sampling of the epilithic diatoms was carried out in the period of May 2021 at two locations, first before and second after the Lukavac city, which is known as industrial city in Bosnia and Herzegovina. Taxonomic verification was carried out using light microscope on permanent slides and revealed in total 38 diatom taxa. The genera with the largest number of species were Diatoma, Navicula and Nitzshia. Identified taxa are widespread species that are known for tolerating  high levels of pollution. A slightly higher number of taxa was recorded at the first location, while at the second, a higher proportion of halophilic taxa was observed.

Denis Čaušević, Semir Mašić, Ivor Doder, Kęstutis Matulaitis, Seth Spicer

Background. As basketball constantly evolves, physical performance becomes more and more important. Physical fitness assessments are the most reliable way to find out at what level a basketball player is prepared to perform. Therefore, the main aim of this research was to determine if the speed, agility and power of under-16-year-old (U16) basketball players is related to their playing positions. Methods. The study included (n = 40) young basketball (aged 14.99 ± 0.84 years) players. The variables included height, body mass, body mass index (BMI), fat-free mass (FFM), the percentage of body fat (BF%), the counter movement jump (CMJ), counter movement jump with free arms (CMJ free arms), squat jump (SJ), 5m, 10m, 15m and 20m sprints, T-test, Illinois test and 505 test. Results. The results showed that centers are taller and heavier than other positions, while the speed, agility and power of forwards are greater than other positions. Conclusions. At the youth basketball level there are differences in anthropometric and physical fitness testing results between positions. This supports the fact that athletes may be more likely to be selected for a given position based on how their anthropometric and athletic abilities pair with the demands of a given position. Keywords: body composition; physical testing; playing position; performance.

Milena Mlakić, I. Odak, Ivan Faraho, M. Bosnar, M. Banjanac, Z. Lasić, Ž. Marinić, Danijela Barić et al.

In this research, the synthesis, photochemistry, and computational study of new cis- and trans-isomers of amino-thienostilbenes is performed to test the efficiency of their production and acid resistance, and to investigate their electronic structure, photoreactivity, photophysical characteristics, and potential biological activity. The electronic structure and conformations of synthesized thienostilbene amines and their photocyclization products are examined computationally, along with molecular modeling of amines possessing two thiophene rings that showed inhibitory potential toward cholinesterases. New amino-styryl thiophenes, with favorable photophysical properties and proven acid resistance, represent model compounds for their water-soluble ammonium salts as potential styryl optical dyes. The comparison with organic dyes possessing a trans-aminostilbene subunit as the scaffold shows that the newly synthesized trans-aminostilbenes have very similar absorbance wavelengths. Furthermore, their functionalized cis-isomers and photocyclization products are good candidates for cholinesterase inhibitors because of the structural similarity of the molecular skeleton to some already proven bioactive derivatives.

K. Ram, M. Serdar, D. Londono-Zuluaga, K. Scrivener

The aim of this study is to evaluate how much the changes in the concrete mix design, which enable carbon footprint reduction, are impacting mechanical properties and predicted service life of concrete structure. The starting point of this study was concrete mix used in a recent reinforced concrete Pelješac Bridge in the Adriatic. In the first round of experiments the amount of cement in this initial mix was significantly lowered, without jeopardising workability of the mix. In the second round, the main part of the cement was substituted with the combination of fly ash and limestone or calcined clay and limestone. All supplementary cementitious materials used were sourced in the region of the structure. The calcined clays used in this study were collected locally and found to have a low kaolin content. On all mixes fresh and mechanical properties were tested to ensure that the requested equal or better workability and mechanical stability were reached. Furthermore, on each mix chloride migration was tested to evaluate the resistance of mix to chloride penetration. All mixtures were evaluated based on the overall performance considering mechanical, durability, and carbon footprints. The results indicate that the total cement content had a significant effect on durability and thus service life. The bridge mix design was determined to be ‘over designed,’ as all alternative mixes achieved a similar or higher sustainability index with lower amount of cement.

Çiler Hatipoğlu, Nihada Delibegović Džanić, Elżbieta Gajek, Lina Miloshevska

The popularity of online crowdsourcing platforms was slowly increasing among language learners before the pandemic, but COVID-19 changed the educational systems worldwide. This study aims to uncover whether or not, and if ‘YES’, how the attitudes and habits of language learners concerning the use of crowdsourcing materials in Turkey, Bosnia and Herzegovina, the Republic of North Macedonia and Poland changed during the pandemic. To compare the pre-and during the covid crowdsourcing tool usage, the cross-culturally appropriate questionnaire utilised in the pre-COVID-19 period was used again. The collected data were analysed qualitatively and quantitatively to identify the differences between the periods. The study’s findings showed that the shift from face-to-face to online learning significantly affected the development of crowdsourcing platforms worldwide and their employment in the studied countries. The results also demonstrated that a combination of factors, such as reduced interactions with teachers and peers, an increase in workload, and a lack of support on the part of institutions, led to students taking responsibility for their learning. The number and characteristics of the popular platforms changed from country to country since expectations from students varied.

In oral and written communication there exist various linguistic tools by which one tells or asks their interlocuter to do something. In such cases, one most often uses the imperative, even though in different languages there are different ways to attain a goal, i.e., to realize the intention or request of the message sender. In Latin, there existed along with the imperative a mood called the subjunctive through which speakers could express commands or prohibitions, but also suggestions, desires, requests, counsel, or encouragement for the message receiver to complete a certain act. The content mentioned can be expressed using the present (e.g., You are shutting up now!) or the future tenses (e.g., You are going to the library tomorrow!), or with certain periphrastic forms (e.g., This job ought to be finished!). The form, then, does not always correspond with the anticipated content; that is, one type of content can be expressed using various forms. This paper is directed at the observation and analysis of the various forms and functions of commands used by Cicero in his epistles, as well as on their classification according to which of them prevail in individual groups of letters. Along with this, seeing as in some examples ambiguous forms of the imperative are found, whose function is conditioned due to various pragmatic parameters and contextual frameworks, the goal is to also to point out the importance of context and its elements, especially those of a socio-political, cultural or historical environment and the role of participants in the choice, usage, and interpretation of forms of the command.

Introduction: Laboratory personnel (LP) represent a high-risk group of healthcare workers for whom the primary laboratory environment and specific work activities are a major source of potential exposure to health hazards. This study aimed to evaluate the developed matrix and assess risk based on self-assessment. Methods: This multicenter, qualitative, and cross-sectional study was conducted on LP employed in biomedical laboratories. The respondents were divided into groups according to their territorial affiliation. The data collection tool used was a six-area questionnaire distributed online through a network of professional associations. For the risk assessment, a matrix was developed with scores ranging from 0 to 650, dividing the risk level into four categories. Descriptive and inferential statistical methods were used for the statistical analysis. Results: The developed model combined the classification of risk and risk factors with a certainty of p < 0.001. The regression analysis showed that working conditions had the greatest influence on overall risk, followed by physical, biological, and physical hazards. Of the 640 respondents, the medium risk category was the highest in European Union (EU) countries (81.2%). Comparing the values in the high-risk category between the Bosnians and Herzegovinians (BiH) group and the Republic of Serbia, Republic of Northern Macedonia, and Montenegro (SCM) group with the EU group, a doubling (16.6%: 36.7%) and tripling (16.6%: 52.1%) of the proportion was found, respectively (p < 0.001). Overall, 1.7% of the LPs from BiH fell into the high-risk category. Conclusions: The designed matrix provides a reliable basis for identifying risk predictors in the study population and can serve as a useful tool for conducting risk assessments in biomedical laboratories. The results of the risk assessment indicate significant differences between the studied groups and highlight the need for increased control of BiH workplaces through new regulatory requirements.

Introduction: information and communication technology (ICT) has multiplied its availability and use, resulting in enormous usage, especially among school-age children, who use it for an average of 7.5 hours per day. The amount of time adolescents spend in front of electronic screens has increased significantly between the ages of 10 and 14, but there are significant gender changes. For boys, this increase is 41.6 minutes per day, while for girls it is 22.7 minutes per day. Methods: The study is designed as an epidemiological, cross-sectional, descriptive-analytic, comparative study. The study will be conducted in the form of an online questionnaire on the Microsoft Forums platform during the period 12/15/2021 to 12/30/2021. Respondents were able to access the request for consent using a link and a QR code, which was previously signed by a parent/guardian giving consent for children to participate in this study. Results: The results of this study show that school-aged children generally use cell phones with a touch screen between 2 and 3 hours daily during the work week (39.2%) and TV (39.5%). Pain caused by ICT use in the past 12 months occurred mainly in the neck/shoulder area (42.4%), while pain in the previous month occurred mainly in the lower extremities (29.1%). Conclusion: Increased use of ICT devices was associated with higher levels of musculoskeletal symptoms. The higher pain burden in various body parts is of concern, and further research on the effects of ICT device use in adolescents is needed and warranted.

A. Landini, P. Timmers, A. Frkatović-Hodžić, I. Trbojević-Akmačić, F. Vučković, T. Pribic, Regeneron Genetics Center, G. Tzoneva et al.

It is often difficult to be certain which genes underlie the effects seen in association studies. However, variants that disrupt the protein, such as predicted loss of function (pLoF) and missense variants, provide a shortcut to identify genes with a clear biological link to the phenotype of interest. Glycosylation is one of the most common post-translationalmodifications of proteins, and an important biomarker of both disease and its progression. Here, we utilised the power of genetic isolates, gene-based aggregation tests and intermediate phenotypes to assess the effect of rare (MAF<5%) pLoF and missense variants from whole exome sequencing on the N-glycome of plasma transferrin (N=1907) and immunoglobulin G (N=4912), and their effect on diseases. We identified significant gene-based associations for transferrin glycosylation at 5 genes (p<8.06x10-8) and for IgG glycan traits at 4 genes (p<1.19x10-7). Associations in three of these genes (FUT8, MGAT3 and RFXAP) are driven by multiple rare variants simultaneously contributing to protein glycosylation. Association at ST6GAL1, with a 300-fold up-drifted variant in the Orkney Islands, was detectable by a single-point exome-wide association analysis. Glycome-associated aggregate associations are located in genes already known to have a biological link to protein glycosylation (FUT6, FUT8 for transferrin; FUT8, MGAT3 and ST6GAL1 for IgG) but also in genes which have not been previously reported (e.g. RFXAP for IgG). To assess the potential impact of rare variants associated with glycosylation on other traits, we queried public repositories of gene-based tests, discovering a potential connection between transferrin glycosylation, MSR1, galectin-3, insulin-like growth factor 1 and diabetes. However, the exact mechanism behind these connections requires further elucidation.

27. 12. 2022.
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Adil Zulfikarpašić, Put u Foču, Sarajevo: Bošnjački institut – Fondacija Adila Zulfikarpašića, 2022. 140 str., ISBN 978-9958-743-43-6.

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