The world’s leading health institutions warn that adolescents do not meet the minimum level of physical activity necessary for the proper physical development of young people, which is considered one of the main problems of public health. In addition to personal preference, environmental and cultural factors play an important role in the involvement of young people in sports. This study aimed to examine potential differences in involvement in sports, anthropometric characteristics and motor abilities, in adolescents of different residential statuses. The sample included 83 adolescents, of whom 45 (54.2%, 13.51±0.55 yrs) were from urban areas and 38 (45%, 13.45±0.50 yrs) were from rural areas in the Belgrade region. Surveying detected their involvement in sports, and anthropometric characteristics were assessed through body height (BH) body mass (BM), body mass index (BMI), and motor abilities through explosive strength of lower and upper extremities and agility. A Mann-Whitney U test has shown that place of residency impacts involvement in sports and motor abilities, while there was no impact on anthropometric characteristics. Cohen’s criteria (r) detected the level of impact. Adolescents from urban areas are more involved at sports and have better motor abilities. There is no difference in anthropometric characteristics. Considering the relatively small sample of respondents and variables assessed, the obtained results can hardly be generalized. However, this does not diminish the importance of the present study which examined the urban-rural state of sports activity, anthropometric characteristics and motor abilities of adolescents in Serbia, thus making an important contribution to this field.

A growing issue on a global scale is the emergence of helminth species and populations that are resistant to one or more anthelmintics. The majority of currently available anthelmintics used to control parasitic nematodes of cattle and sheep belong to only three main groups, benzimidazoles, imidazothiazoles and avermectins/milbemycins. The availability of reliable and precise techniques for its identification and monitoring is a critical component of the success of helminth control programs intended to prevent the spread of resistance in nematode populations. In vivo method like fecal egg count reduction test and in vitro methods such as egg hatch assays, larval motility test, larval development test and polymerase chain reaction (PCR) can be used for the detection of anthelmintic resistance although each has some reliability, repeatability, sensitivity, and ease of interpretation issues. The genetic basis of resistance to the majority of anthelmintics are still not well understood. Thanks to recent developments in high-throughput sequencing, it is now possible to define features such as drug resistance using genome-wide techniques. Keywords: Anthelmintics; Helminths; Resistance; Detection assays; Molecular diagnostics; Parasite control

Chronic myeloid leukemia (CML) is a hematological disorder characterized by increased proliferation of the granulocytic cell lineage. We diagnose CML by presence of the breakpoint cluster region-abelson (BCR-ABL1) oncogene using the quantitative real-time polymerase chain reaction (QRT-PCR). This method provides an accurate and unambiguous follow-up of the treatment response to tyrosine kinase inhibitors (TKIs). This study aimed to determine the molecular response to the first and second generation of TKIs in first and second line of treatment using the QRT-PCR method. We conducted a retrospective study on 48 CML patients treated with the first and second generation of TKIs in first and second-line treatment. Treatment responses have been followed-up every 3 months using the QRT-PCR method. Patients were divided into three groups according to molecular responses to the first line of TKIs. Results obtained in this study showed that the first group of patients did not achieve major molecular response (MMR) in the first 18 months of TKI treatment. The second and third group of patients achieved MMR and deep molecular response (DMR) in the first 18 months of TKI treatment. These results indicate that patients with MMR and DMR in the first 18 months of TKIs treatment had a favourable clinical course of the disease. Inadequate molecular responses to the first line of TKIs can be improved with in increase of the dose of TKIs or by switching to other TKIs. Continuous and timely molecular monitoring of TKI’s response in CML patients provides a careful observation of the disease's course and a proper treatment approach.

Background Understanding the anatomy and morphological variability of the orbital region is of great importance in clinical practice, forensic medicine, and biological anthropology. Several methods are used to estimate sex based on the skeleton or parts of the skeleton: classic methods and the geometric morphometric method. The objective of this research was to analyse sex estimation of the orbital region on a sample of skulls from a Bosnian population using the geometric morphometric method. Materials and Methods The research was conducted on three-dimensional models of 211 human adult skulls (139 males and 72 females) from the Osteological Collection at the Faculty of Medicine in Sarajevo. The skulls were recorded using a laser scanner to obtain skull 3D models. We marked 12 landmarks on each model to analyse sexual dimorphism. Landmarks were marked using the program Landmark Editor. After marking the landmarks, we used the MorphoJ program to analyse the morphological variability between male and female orbital regions. Results After Procrustes superimposition, generating a covariant matrix, and introducing sex as a variable for classification, a discriminant functional analysis (DFA) was applied which determined the estimation for males with 86.33% accuracy and for females with 88.89% based on the form of the orbital region. The results of regression analysis showed that the size of the orbital region has a statistically significant effect on its shape's sexual dimorphism. After excluding the influence of size and providing DFA, we concluded that sex estimation was possible with 82.01% accuracy for males and 80.55% accuracy for females based on the shape of the orbital region in the examined sample. Conclusion Sex estimation based on the orbital region was possible with more than 80% accuracy for both sexes, which is a high percentage of correct estimation. Therefore, we recommend using the orbital region of the skull for sex estimation.

In this study, using the methods of spatial autocorrelation in geographic information systems (GIS), an analysis of the spatial distribution of mortality rates in Bosnia and Herzegovina was performed in order to identify high-risk areas with increased mortality and depopulation. Spatial analysis is based on the calculation of global (Global Moran’s I and Getis-Ord General G) and local (Anselin Local Moran’s I and Getis-Ord Gi*) statistical indices of spatial autocorrelation. The values of global statistical indices confirmed a clustering of high mortality values, while local statistical indices confirmed high mortality rates in the municipalities of northwestern and eastern Bosnia and Herzegovina. Conducted research provides an insight into spatial patterns of demographic processes, while results obtained by this research are significant for demographic development and future spatial demographic research in Bosnia and Herzegovina.

This study aimed to explore the use of nominal and verbal Anglicisms by Bosnian high school students, the students’ familiarity with these Anglicisms and their attitudes towards them and it also aimed to see whether these factors vary with respect to the frequency of students’ use of English in speaking, listening, reading and writing. The findings point to a very frequent use of Anglicisms among Bosnian participants, particularly verbal Anglicisms which tended to be significantly more frequently employed than nominal Anglicisms, and slightly greater familiarity with the English form rather than with the Bosnian equivalent form. Moreover, the frequency of the use of Anglicisms was related to the frequency of reading and writing in English, while the familiarity with the Bosnian equivalent was significantly related to speaking and reading and familiarity with the English equivalent form to all four aspects of English language use. The participants also expressed rather positive attitudes towards Anglicisms, perceiving them as a useful addition and not a serious threat to their native language.

The objective of this paper was to present basic clinical characteristics and outcomes of treating Covid 19 patients during the second wave of the pandemic. In the retrospective study for the period from September 2020 to February 2021 it was analyzed disease history data and radiological lung changes, time from the initial start of the disease until hospitalization, parameters of blood gas analysis, comorbidities, and the outcome. The research covered 409 patients, out of which 263 (64.3%) were males. Average age was 67.07± 12.44 years (min. 20; max. 93). A high comorbidities prevalence (82.9%) was noticed out of which arterial hypertension (69.2%), diabetes mellitus (37.7%) and obesity (24.7%). On the radiological lung scan the most noticed changes were consolidation (46.2%), “ground glass” (41.3%) and interstitial changes (13%). Bilateral lung infiltration was noticed in 91.9% of the patients. Average oxygen saturation was 84.29%±10.28% (min. 35; max. 98; med. 87%). In patients with unilateral lung infiltration, average oxygen saturation was 85.09%±8.60% (med. 89%, min.61% max 98%), while in patients with bilateral lung infiltration average was 84.22%±10.42% (med. 87%, min. 35%, max. 98%). From the total all patients’ death was noticed in 35.7% cases. Morbidity of patients with unilateral lung infiltration was 27.3% and in patients with unilateral infiltration 36.4%. Hospital admission in the first week of the disease indicates the severity of the clinical condition and can be a predictor of poor outcome. Bilateral pulmonary infiltration, obesity and diabetes mellitus are risk factors for high mortality.

Inflammation in the central nervous system (CNS) can impair the function of neuronal mitochondria and contributes to axon degeneration in the common neuroinflammatory disease multiple sclerosis (MS). Here we combine cell type-specific mitochondrial proteomics with in vivo biosensor imaging to dissect how inflammation alters the molecular composition and functional capacity of neuronal mitochondria. We show that neuroinflammatory lesions in the mouse spinal cord cause widespread and persisting axonal ATP deficiency, which precedes mitochondrial oxidation and calcium overload. This axonal energy deficiency is associated with impaired electron transport chain function, but also an upstream imbalance of tricarboxylic acid (TCA) cycle enzymes, with several, including key rate-limiting, enzymes being depleted in neuronal mitochondria in experimental models and in MS lesions. Notably, viral overexpression of individual TCA enzymes can ameliorate the axonal energy deficits in neuroinflammatory lesions, suggesting that TCA cycle dysfunction in MS may be amendable to therapy.

The performance commercial banks in the Croatia have been widely studied, but there is little known about the examination of the nature of competition. For these reasons, it is important to investigate whether the banking sector in Croatia exerts monopoly or monopolistic behavior.  This research empirically explores the nature of competition in the Croatian banking sector by covering 24 commercial banks between 2008 and 2018. In this vein, the panel data analysis was used along with H-Statistics. This study for Croatia and its findings confirms early findings of the existence of monopolistic competition in the most transition and developing countries. In fact, the findings obtained from this study reveals that the commercial banks in Croatia operate under monopolistic structure. Moreover, the results reveal that variable of RIETD, RLTA, RETA, and RDTA are statically significant. It indicates that banks operated in Croatia are focus more on traditional loans than other activities.

In order to determine the pollinizer success rates between twelve apple cultivars in 2021 and 2022, 671 apple embryos were collected from 19 different orchards in Ullensvang (southwestern Norway) and Svelvik (southeastern Norway). Genomic DNA was extracted from the collected embryos and, afterward, a genetic characterization with 15 polymorphic microsatellite markers was conducted. An identical set of markers was also used on all twelve mother cultivars, as well as on six crabapple pollinizers, which were found in the investigated orchards. The obtained molecular data enabled paternity analyses to be performed with the objective of assigning a male parent to each embryo. The paternity analyses identified pollen donors for all, except for 3% of the embryos. In most cases, it was possible to identify the most successful pollinizers for each cultivar, with ‘Aroma’ and ‘Discovery’ being the most efficient pollen donors overall. Tree abundance seems to be a major factor in pollinizer success, while semi-cross-compatible characteristics represent a hindrance. Only 7% of the analyzed embryos were determined to have been fertilized by pollinizers outside the orchard, confirming the significance of pollinizer proximity for efficient pollination.

In lung adenocarcinoma, antibodies against endogenous retroviruses promote anti-tumour activity, and expression of endogenous retroviruses can predict outcomes of immunotherapy. B cells are frequently found in the margins of solid tumours as organized follicles in ectopic lymphoid organs called tertiary lymphoid structures (TLS)^ 1 , 2 . Although TLS have been found to correlate with improved patient survival and response to immune checkpoint blockade (ICB), the underlying mechanisms of this association remain elusive^ 1 , 2 . Here we investigate lung-resident B cell responses in patients from the TRACERx 421 (Tracking Non-Small-Cell Lung Cancer Evolution Through Therapy) and other lung cancer cohorts, and in a recently established immunogenic mouse model for lung adenocarcinoma^ 3 . We find that both human and mouse lung adenocarcinomas elicit local germinal centre responses and tumour-binding antibodies, and further identify endogenous retrovirus (ERV) envelope glycoproteins as a dominant anti-tumour antibody target. ERV-targeting B cell responses are amplified by ICB in both humans and mice, and by targeted inhibition of KRAS(G12C) in the mouse model. ERV-reactive antibodies exert anti-tumour activity that extends survival in the mouse model, and ERV expression predicts the outcome of ICB in human lung adenocarcinoma. Finally, we find that effective immunotherapy in the mouse model requires CXCL13-dependent TLS formation. Conversely, therapeutic CXCL13 treatment potentiates anti-tumour immunity and synergizes with ICB. Our findings provide a possible mechanistic basis for the association of TLS with immunotherapy response.