Introduction: 2,4-Diaminobutyric acid (2,4-DABA) is an excitatory amino acid with neurotoxic, hepatotoxic, and potentially carcinogenic effects, found in aquatic ecosystems with a tendency to accumulate in plants and animals. Due to its potential impact on development, its embryotoxicity is being studied. Zebrafish (Danio rerio), with high homology to the human genome, serve as an excellent model for studying development and developmental abnormalities. Objective: To investigate the effect of 2,4-DABA on proteins crucial for zebrafish eye development using molecular docking. Methods: Proteome screening was conducted using the FINDSITEcomb software, selecting 1119 proteins based on homology, tissue specificity, and expression timing. Six proteins that met the criteria were analyzed using molecular docking in the AutoDock Vina program. Results: The interaction of the fzd8a protein with 2,4-DABA showed the lowest Gibbs free energy value of -4.6 kCal/mol, while the interaction with the pbx4 protein had the highest value of -3.4 kCal/mol. A similarity was observed in the amino acid sequence of proteins that bind to 2,4-DABA, particularly in those containing an -SH group. Conclusion: 2,4-DABA may affect eye structure development in zebrafish by interacting with proteins throughout the entire development period. The results of in silico analyses provide a basis for further in vivo research, which should be conducted on live embryos to confirm these findings.

Introduction/Aim: Procaine is a local anesthetic from the amino ester group that blocks voltage-gated sodium channels. Since it can cross the placenta in its ionized form, its potential impact on embryonic development is of concern. Zebrafish, which have a significant degree of homology with the human genome, allow for the study of cardiovascular system development with reliable extrapolation to humans. Investigation of the effects of procaine on key proteins involved in zebrafish heart development using molecular docking. Methods: First, a screening of the interaction between procaine and the entire human proteome was performed using FINDSITEcomb software. Based on significant interactions with a high degree of analysis precision, 113 proteins were selected. Using the ZFIN database, the degree of homology between the selected human proteins and zebrafish proteins, tissue specificity, and expression timing were determined. Nine proteins met all the criteria: kcnh6a, kcnh7, kcnh5a, kcnh2a, psen2, rbfa, and zfpl1, and were further investigated through molecular docking in the AutoDock Vina program. Results: Most of the proteins were highly expressed during the blastula stage. Docking results showed that the scn1laa protein and procaine had the lowest Gibbs free energy value (-6 kCal/mol), while the zfp11 protein had the highest value (-4.4 kCal/mol). Procaine binding to the tested proteins revealed similar amino acid sequences within the same protein family. Conclusion: Procaine interacts with proteins involved in zebrafish heart development under in silico conditions. Further analyses on live embryos are needed to complement these findings.

Myocardial infarction (MI) is a serious cardiovascular disease and the primary cause of mortality, with a complex etiopathology. Identifying the genetic basis of myocardial infarction (MI) is essential for developing personalized medical treatments. This study examined the possible association between polymorphisms in the methylenetetrahydrofolate reductase (MTHFR) gene and MI. In the study, 120 patients with MI and 120 age-and-sex-matched controls were genotyped for C677T and A1298C MTHFR polymorphisms by the allele-specific or amplification refractory mutation systempolymerase chain reaction (ARMS-PCR). In the case of the C677T polymorphism, the T/T and C/T genotypes were associated with a significantly increased risk of MI under the dominant genetic model (odds ratio (OR)=2.060; P=0.006). Although there was no significant association between the A1298C variant and MI, this polymorphism was linked to a higher level of creatinine in MI patients (P<0.002). A similar association was observed for the C677T polymorphism (P=0.003). An A-T haplotype represented an increased risk for MI (OR=1.630; P=0.014), whereas the A-C haplotype had a protective role (R=0.517; P=0.002). These findings indicate that C677T MTHFR polymorphism is strongly associated with and increased risk of MI, making it a potential genetic risk factor and a possible predictor of MI.

In medieval Bosnia, the existence of a unified legal code that defined the legal principles, duties, and obligations of the parties involved in a particular dispute was not recorded. One of the most significant relationships in the history of medieval Bosnia was the relationship between the ruler and the nobility. The rights and obligations of the nobles in relation to the ruler in the medieval Bosnian state were defined through the institution of "faithful service" on one side and "noble faith" on the other. Simply put, as long as the nobles faithfully served the ruler, they were secure and their possessions were protected. This article focuses on instances of treason against the ruler and the judicial body responsible for investigating charges and delivering judgments according to established legal customs. The nobles mentioned in the Bosnian charters played a role in defining the relationship between the ruler and the respective noble, as did members of the hierarchy of the Bosnian Church. The Bosnian Church was a religious institution formed in the territory of Bosnia after the displacement of the Catholic diocese in the mid-13th century and was first mentioned in the 1320s. It was considered a heretical movement by the Roman Church, and its followers were excommunicated. It was accepted by the Bosnian ruling dynasty of Kotromanić and by the majority of noble families. From the perspective of neighboring states, the hierarchy of this church was considered to be the authority characterized by exceptional moral values. The aim of this article is to provide the research results regarding the role of the head (djed) of the Bosnian Church in determining the culpability of Bosnian nobles during the 14th and 15th centuries.

This study aims to assess and monitor the health of an urban protected area by analyzing the levels of persistent organic pollutants (POPs) and mercury (Hg) in soil and sediments. Based on the results, the detected concentrations of organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs), polycyclic aromatic hydrocarbons (PAHs), and Hg are above the threshold maximum values for soils, and the prescribed target values for sediments. In the investigated protected area PCBs pose a very high ecological risk. The presence of 16 priority PAHs in analyzed soils and sediments poses a moderate to high cancer risk and Hg poses a considerable health risk to children. The research suggests that preserving urban protected areas is crucial for environmental and urban sustainability. In urban environments these areas should be evaluated in terms of their environmental, eco-geochemical, economic, and socio-cultural dimensions. The value of the existence of this natural oasis lies in its aesthetic and psycho-hydrological impact, local climate regulation, residential isolation, and significant art-architectural and horticultural shaping. The connection between eco-geochemical and management practices, planning, and urban green spaces policy should become an adopted innovation in the cities in the future.

Geopolymer is a type of inorganic polymer which hardens after being synthesized at ambient temperature. Geopolymers are synthesized by the reaction of poly-condensation of the geopolymer precursor and alkali activator. Ecologically, it would be more favourable to use industrial by-products since the hazardous waste is being taken care of. The most frequently used raw materials for geopolymer production are: kaolin clays, meta-kaolin, fly ash, blast furnace slag, fly ash and slag mixture, fly ash and meta-kaolin mixture and so forth. Fly ash from the Stanari Termal Power Plant, granulated slag from ArcelorMittal Zenica and quarc sand from Tuzla were used as initial raw material for the purpose of the study presented in this work. Based on the results obtained through research carried out in laboratory conditions at the Faculty of Engineering and Natural Sciences in Zenica and at the "Kakanj" Cement Factory, it can be concluded that fly ash from the TPP Stanari together with granulated blast furnace slag from ArcelorMittal Zenica can successfully use for the production of geopolymer. This test determined that the best compressive strength results were obtained by samples with a ratio of water glass/NaOH=2.0 and that the optimal heat treatment temperature was 60°C.

The discovery and introduction into practice of various substances that have a harmful effect on insects, known as insecticides, have undoubtedly achieved great, and one might even say grandiose, results in protecting people, animals and plants from numerous insects. As is known, their application eradicated certain infectious diseases, facilitated the suppression and treatment of ectoparasitises in humans and animals, and improved and increased agricultural production. In order to increase agricultural production, a large amount (millions of tons) of these funds are used in the world on an annual basis. This is primarily due to the prevailing opinion that agricultural production has become practically impossible without the use of insecticides and other pesticides. The facts just mentioned, that is, this kind of application of insecticides, which is often irrational, is causing increasing concern. In addition to the increasing development of resistance, and thus the decrease in the effectiveness of insecticides, there is also a growing concern about environmental pollution. There are not rare cases everywhere, even here in our country, that non-target insects, that is beneficial insects, ie. bees, but also other organisms in soil and water. The goal of this paper is to point out not only the possible harms of irrational use of insecticides, but also to list some of the insecticides that appeared on the market at the end of the last and the beginning of this century, and those of plant origin as potential substitutes for insecticides that are currently used.

During the three-year field research (2019-2022) in the area of the Canton of Sarajevo, we discovered many invasive plant species in localities where they had not been recorded before. Among them, Ailanthus altissima (Mill.) Swingle, Ambrosia artemisiifolia L. and Reynoutria japonica Houtt. stand out in particular due to their abundance and coverage. This study aimed to provide data on the localities where these invasive plants were found, including altitude, longitude and latitude, soil type and their floristic and phytocoenological features. Data from these localities should contribute to the creation of a database that can be used for more a efficient fight against the aforementioned invasive species. In total, 42 new localities were recorded for Ailanthus altissima, 10 for Ambrosia artemisiifolia and 15 for Reynoutria japonica. Due to their abundance and appearance, Ailanthus altissima and Reynoutria japonica have already become a big problem for urban horiculture in Sarajevo, while Ambrosia artemisiifolia poses a health concern, and therefore, urgent measures and necessary to remove them.

Ragweed is a neophyte which inhabits various crops and ruderal habitats. That is the reason why this paper is dedicated to phytocenology and ecological relations of ragweed in such a habitat. Research was carried out in the area of a former railway freight and customs terminal, in the period from 2021 to 2023. Phytocenological image was made and all the parameters were assessed: life form, phytocenological affiliation, floral elements, indicator values, and soil analysis. The aim of the paper is to present the places where ragweed may be found, as well as under what ecological conditions and with what other species it occurs. This research yielded new data on the phytocenology of ragweed, determined in the vegetation of the order Chenopodietalia. A total of 69 species from 23 families were identified. The most numerous is the Asteraceae family. Phytocenologically, the largest number of species belongs to the order Chenopodietalia, and as far as the indicator values are concerned, the most numerous are the representatives of tertiary vegetation. Hemicryptophytes predominate regarding life forms, and concerning the floral elements, 40 different elements from 9 groups were noted. The most numerous group are Eurasian species, and the most numerous individual floral elements are euras-smed and eurassubozean-smed.

Here we demonstrate quasi-phase-matched up- and down-conversion in a periodically poled van der Waals semiconductor (3R-MoS2). This work opens the new and unexplored field of phase-matched nonlinear optics with microscopic van der Waals crystals.

The aim of this paper is to identify models for preventive risk recognition as tool for crine prevention in the renewable energy sector through a systematic review of the available literature. These models should represent a formalized set of steps or protocols that can be used at any stage of renewable energy projects, particularly in the construction of renewable energy power plants, to mitigate and minimize the risk of criminal offenses. Models of preventive risk recognition ensure that the power plant construction project progresses smoothly from start to finish without unnecessary financial losses due to their ability to identify all weak points in the entire process. With the help of the models described in this paper, the transition to green energy is accelerated and sustainable development is fully supported,

This paper examines the complex landscape of digital currencies, non-fungible tokens (NFTs), and distributed ledger technology (DLT), focusing on their implications within the accounting and financial reporting sector. The surge in popularity of these assets has brought about reporting challenges and complexities. The lack of comprehensive accounting standards and the digitization of financial reporting processes further compound the situation. These challenges underscore the need to update accounting practices to align with the security and transparency offered by DLT. The study examines the International Financial Reporting Standards (IFRS) for digital currency reporting, analysing their implications and potential solutions for the accounting community. Central to this exploration is the question: How can the accounting sector navigate the multifaceted challenges and harness the multifarious opportunities that stem from digital currencies, NFTs, and DLT? Using a comprehensive research approach, including a literature review, empirical analysis, case studies, and comparative analysis, this study identifies strategies for managing the reporting complexities of digital assets. It also highlights the importance of collaborative dialogue between stakeholders and regulators to ensure consistency in an evolving landscape. This paper guides the accounting and investment sector in making informed decisions, fortified by a nuanced understanding of the evolving digital asset terrain.