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Publikacije (37582)

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V. Jurišić, G. Bogdanovic, T. Srdić, N. Kraguljac, A. Kerenji, M. Baltić, V. Baltic

Z. Ipekci, A. Altinkut, K. Kazan, K. Bajrovic, N. Gozukirmizi

High frequency of plant regeneration from Paulownia elongata was obtained on Murashige and Skoog (MS) medium and Woody Plant Medium (WPM), with appropriate supplements of growth regulators. Leaves, leaves with petioles, internodes and nodes excised from 3-month-old non-aseptically grown P. elongata were used as explants. The highest shoot regeneration efficiency (93.7%) was obtained from the nodes of P. elongata on MS medium supplemented with 0.1 mg/ml naphthaleneacetic acid (NAA) and 1 mg/ml 6-benzylaminopurine (BAP). The highest root formation efficiency (100%) from the regenerated shoots was obtained on WPM supplemented with 1 mg/ml indolebutyric acid (IBA). Rooted plantlets were transplanted to soil with a survival efficiency of almost 100%. The regeneration system reported here could be useful for rapid multiplication of elite genotypes of P. elongata in a short period of time.

P. Schrade, H. Klein, I. Egry, Z. Ademovic, D. Klee

Density measurements of aqueous albumin solutions as a function of concentration and temperature are reported. The solvents were H(2)O, D(2)O, and a physiological H(2)O-based buffer. An anomaly of the density at very small concentrations of albumin in D(2)O was found. Furthermore, the partial specific volume of albumin is remarkably different in D(2)O and H(2)O. We attribute both effects to structural differences of the solvents. Copyright 2001 Academic Press.

A. Rajakumar, Kerry A. Whitelock, L. Weissfeld, A. Daftary, N. Marković, K. Conrad

Transcription factors orchestrate the development of extraembryonic tissues. Because placental hypoxia likely plays an important role in both normal and abnormal placentation, we have been investigating the hypoxia-inducible transcription factors (HIFs) in the human placenta. In this report, we focus on the placentas from women with preeclampsia. Because the placenta is a large, heterogeneous organ, we employed a systematic and unbiased approach to placental sampling, and our results are based on the analyses of eight biopsy sites per placenta. We observed no significant differences in HIF-1a or -2a mRNA expression between normal term and preeclamptic placentas. Nor was HIF protein expression significantly different, with the notable exception of HIF-2a, which, on average, was increased by 1.7-fold in the preeclamptic placentas (P , 0.03 vs. normal term placentas). Considering all 48 paired placental biopsy sites (eight sites each for six normal term and six preeclamptic placentas), HIF-2a protein levels in the preeclamptic placentas exceeded those in the normal term placentas in 39, or 81%, of the paired sites (P , 0.0013). The HIF-2a immunoreactivity was mainly located in the nuclei of the syncytiotrophoblast and fetoplacental vascular endothelium in the preeclamptic villous placenta. To control for the earlier gestational age of the preeclamptic placentas, an additional group of placentas from preterm deliveries without preeclampsia were also evaluated. The HIF protein expression was comparable in these preterm specimens and the normal term placentas. We conclude that protein expression of HIF-2a, but not of HIF-1a or -1b, is selectively increased in the preeclamptic placenta. The molecular mechanism(s) of this abnormality as well as the genes affected downstream are currently under investigation. To our knowledge, this is the first report of abnormal HIF-2a expression in human disease other than cancer. developmental biology, gene regulation, placenta, syncytiotrophoblast, trophoblast

R. Škrbić, Dragana Babić‐Djurić, Svjetlana Stojisavljević‐Šatara, Nataša Stojaković, L. Nežić

Using ATC/DDD methodology, we analyzed antibiotic utilization in the Clinical Centre of Banja Luka, one of the largest clinical centres in Bosnia and Herzegovina, during the war and postwar period (1994–2000), as well as the role of drug donations on doctors' prescribing decisions. The retrospective analysis of antibiotic utilization (group J according to the Anatomical Therapeutical Chemical – ATC classification) was based upon the data provided from the hospital computer centre and calculated as the number of defined daily doses (DDD) per 100 bed days. The pharmacoepidemiological analysis showed that the total use of antibiotics changed markedly; in the war year of 1994, as well as in 1998, antibiotics were the second most frequently used group of drugs (19.7% and 14.1% of total drug utilization respectively), while in the following years antibiotics were considerably less used. These dynamics were significantly influenced by drug donations, the percentage of which in the overall antibiotic supply in 1996 was 91.5%, while in 1999 and in 2000 it decreased considerably to 46.8% and 45.6%, respectively. The most widely prescribed antibiotics were penicillins, aminoglycosides, sulphonamides and tetracyclines. Among these, the aminopenicillins, co‐trimoxazole, gentamicin and tetracyclines were mainly (70–100%) supplied as a drug donations. However, macrolides, cephalosporins and quinolones were less used due to fact that they were considerably less often delivered through drug donations. It can be concluded that the drug donations had a significant impact on prescribing practice and the rational use of antibiotics in the Clinical Centre studied.

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