Lilium martagon L. var. cattaniae Vis. (Liliaceae) is endemic plant of Dinaridi mountain. In this work we established protocol for fast in vitro propagation and multiplication of Lilium martagon var. cattaniae. The aim was to enable fast production of plant material as potential source of pharmaceutically valuable secondary metabolites. Seeds of L.martagon var. cattaniae were germinated on a Murashige and Skoog basal medium with a supplement of 0.15 mg/l gibberellic acid (GA3), and multiplication was performed on MS medium supplemented with 0.1 mg/l gibberellic acid (GA3), 0.2 mg/l indole-3-butyric acid (IBA) and 0.5 mg/l 6-ben- zylaminopurine (BAP). We used ultrasound assisted extraction to prepare extracts of leaves and bulbs of Lilium martagon var. cattaniae, which were evaluated for their genotoxic potential using Allium test and cytokinesis-block micronucleus test in human lymphocytes culture. There was statistically significant difference between all used concentrations of lilium extracts and control on proliferation of cells of root tip of onion (Allium cepa). In cytokinesis-block micronucleus test no statistically significant difference between frequencies of analyzed parameters in samples treated with tested concentrations of extracts and control was obtained.
SUMMARY The development of modern technology and the Internet has enabled the explosive growth of distance learning. distance learning is a process that is increasingly present in the world. This is the field of education focused on educating students who are not physically present in the traditional classrooms or student’s campus. described as a process where the source of information is separated from the students in space and time. If there are situations that require the physical presence of students, such as when a student is required to physically attend the exam, this is called a hybrid form of distance learning. This technology is increasingly used worldwide. The Internet has become the main communication channel for the development of distance learning.
UDK 546.19:543.422.3 Razvijena je jednostavna i jeftina metoda za određivanje arsena u niskom ppb području. Metoda je zasnovana na redukciji arsenita i arsenata u kiseloj reakcionoj sredini do gasovitog produkta hidrida arsena, arsina i njegovoj reakciji sa živa(II) bromidom. Živa(II) bromid je impregniran na čvrsti nosač (filter papir), a formiranje i detekcija žuto-smeđeg produkta je vršena spektrofotometrijski, upotrebom instrumenta za terensko određivanje arsena, Supralab FD. Za podešavanje kiselosti rastvora korištena je amidosulfonska kiselina, a redukcija arsena je vršena natrij borhidridom. Metoda je korištena u određivanju arsena u uzorcima zemljišta nakon digestije. Validacija metode je izvršena upotrebom standardnog referentnog materijala (SRM 2710) i interlaboratorijskom komparacijom analizom sa ICP-MS. Limit detekcije razvijene metode je 0,06 mg As/dm3 sa limitom kvantifikacije od 0,2 mg As/dm3.
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