This is the first study performed in population from Bosnia & Herzegovina (BH), in which we analysed a significance of genetic variations in drug-metabolising enzyme, cytochrome P450 (CYP), in pathogenesis of Type 2 diabetes. We have determined allele frequencies for CYP2C9*2, CYP2C19*2, and CYP2D6*4 in diabetic patients and nondiabetic controls. Genomic DNA was extracted from blood samples collected from 37 diabetic and 44 nondiabetic subjects. A real-time polymerase chain reaction was used for the detection of specific CYP polymorphisms, with the application of the specific TaqMan® SNP genotyping tests (Applied Biosystems). Interestingly, results from this study have demonstrated that frequencies of CYP2C19*2 and CYP2D6*4 variants were in line, while frequency of CYP2C9*2 polymorphism seemed to be lower in this sample of BH population as compared to the Caucasians genotype data. Furthermore, no significant difference in allele frequencies for CYP2C9*2, CYP2C19*2, and CYP2D6*4 was demonstrated between diabetic and nondiabetic subjects. Thus, results form this study seem to indicate no relationship between CYP2C9, CYP2C19, and CYP2D6 genotype and diabetes susceptibility in Bosnian population. This in part may reflect a limited study population included in our study and would require larger cohorts to reveal potential relationships between analysed CYP genetic variants and diabetes risk. In addition, it would be pertinent to further explore possible effects of CYP genetic variations on therapeutic and adverse outcomes of oral antidiabetics, which might be the key in optimising therapy for individual patient with Type 2 diabetes.

Genus Artemisia is thought to have reached the Americas across the Bering Strait from Asia during the late Tertiary, but the systematic position of the South American endemic species and the migration routes towards the south have not yet been studied. We used nuclear DNA sequences to unravel the interspecific relationships among the South American Artemisia and their connections with the remaining species of the genus, as well as using fluorescent in situ hybridisation and genome size assessments to characterise this polyploid complex. Most of the species are clustered in a monophyletic clade, nested within the American endemic clade, with the exception of A. magellanica Sch. Bip., which appears segregated from the other American species and constitutes a clade together with A. biennis Willd. Fluorescent in situ hybridisation and genome size data revealed that monoploid genome size remains quite constant across ploidy levels and a proportional increase of ribosomal loci was detected, a dynamic not usually found in this genus. The results are discussed in the light of evolutionary processes which occur in plants, and plausible origins for the South American endemic species are hypothesised.

We present explicit formulae for the eccentric connectivity index and Wiener index of 2-dimensional square and comb lattices with open ends. The formulae for these indices of 2-dimensional square lattices with ends closed at themselves are also derived. The index for closed ends case divided by the same index for open ends case in the limit N →∞ defines a novel quantity we call compression factor. This factor was calculated for both eccentric connectivity and Wiener index for 2- dimensional square lattice.

The paper also synthesizes a feasible process structure that can be applied for the process of fractional crystallization, and gives a simulation of the process by calculating the material balance of the process. The parameters of relevant process paths that were obtained prove that the process presented in this paper is feasible in practice and applicable in industry.

Slavonian Kulen (SK) is traditional dry sausage produced in the region of Slavonia in eastern Croatia. It is made from mince of pork, back fat, spices and salt filled into pork cecum. After stuffing, the sausages are cold-smoked and ripened-dried afterward for several months. Present work aimed to analyse some physicochemical and organoleptic traits of ripe SK as well as the safety of final product. The SK samples (n=12) from different small-scale manufactures were analysed. The following physicochemical attributes were recorded: moisture 38.2%±3.6, protein 35.0%±3.1, fat 23.7%±4.6, moisture/protein ratio 1.1±0.1, pH value 5.37±0.23 and water activity (aw) 0.82±0.02. Mean organoleptic scores, on five-point scale, were 3.7±0.6 for surface appearance, 3.4±0.6 for surface smell, 3.8±0.5 for consistency, 3.2±0.4 for inner smell, 3.0±0.7 for cross section quality, 3.3±0.5 for texture, 3.1±0.4 for taste and aroma, 3.0±0.5 for after taste and 3.2±0.4 for overall quality. Regarding the product safety, the following results (per kg) were determined: histamine 330.8 mg±126.3, tyramine 233.9 mg±124.7, nitrite 6.55 mg±3.88 and benzo(a)pyrene 0.05 たg±0.03. Salmonella spp. and L. monocytogenes have not been found in any sample while counts of S. aureus, enterobacteria and sulfite-reducing clostridia were in accordance with regulations.