Introduction. At the end of 2019 and the year before, there was a significant spread of measles in the World Health Organization (WHO) European Region.Gap statement. Among the countries that reported, a measles outbreak was Bosnia and Herzegovina (BiH).Aim. To describe the measles outbreak in BiH (an entity of the Federation of BiH, FBiH) in 2019.Methodology. Confirmatory IgM serology, measles nucleic acid detection by real-time RT-PCR and virus genotyping were done in the WHO-accredited laboratory for measles and rubella at the Clinical Center of the University of Sarajevo, Unit for Clinical Microbiology. Genotype was determined in all measles-RNA-positive cases by sequence analysis of the 450 nt fragment coding the C-terminal of measles virus nucleoprotein (N).Results. From 1 January to 31 December 2019, 1332 measles cases were reported, with the peak observed in April 2019 (413/1332, 31.01 %). Sarajevo Canton had the highest incidence, number of cases and percentage (206.4; 868/1332; 65.17 %) of measles cases. Around four-fifths of infected persons were unvaccinated (1086/1332, 81.53 %), while 4.58 % of the patients (61/1332) were immunized with one dose of measles-containing vaccine. The highest proportion of cases was found in children 0-6 years of age (738/1332, 55.41 %). Measles IgM positivity was determined in 75.88 % (346/456), while virus RNA was detected in 82.46 % (47/57) of the swab samples. All measles virus sequences belonged to genotype B3. SNP (position 216: C=>T) was detected in 1 of the 40 sequences obtained during this outbreak.Conclusion. Due to suboptimal immunization coverage, BiH belongs to countries at a high risk for measles outbreaks. Post-COVID-19 (coronavirus disease 2019) pandemic, targeted and tailored strategies are required to ensure routine vaccination demand and acceptance and broad partner and stakeholder group participation.

Background. Autism spectrum disorder (ASD), attention-deficit/hyperactivity disorder (ADHD), and schizophrenia (SCZ) are highly heritable and linked to disruptions in foetal (neuro)development. While epigenetic processes are considered an important underlying pathway between genetic susceptibility and neurodevelopmental conditions, it is unclear (i) whether genetic susceptibility to these conditions is associated with epigenetic patterns, specifically DNA methylation (DNAm), already at birth; (ii) to what extent DNAm patterns are unique or shared across conditions, and (iii) whether these neonatal DNAm patterns can be leveraged to enhance genetic prediction of (neuro)developmental outcomes. Methods. We conducted epigenome-wide meta-analyses of genetic susceptibility to ASD, ADHD, and schizophrenia, quantified using polygenic scores (PGSs) on cord blood DNAm, using four population-based cohorts (npooled=5,802), all North European. Heterogeneity statistics were used to estimate DNAm pattern overlap between PGSs. Subsequently, DNAm-based measures of PGSs were built in a target sample, and used as predictors to test incremental variance explained over PGS in 130 (neuro)developmental outcomes spanning birth to 14 years. Outcomes. Probe-level analyses showed SCZ-PGS associated with neonatal DNAm at 246 loci (p<9x10-8), predominantly in the major histocompatibility complex. Functional characterization of SCZ-PGS loci confirmed strong genetic effects, significant blood-brain concordance and enrichment for immune-related pathways. 8 loci were identified for ASD-PGS (mapping to FDFT1 and MFHAS1), and none for ADHD-PGS. Regional analyses indicated a large number of differentially methylated regions for all PGSs (SCZ-PGS: 157, ASD-PGS: 130, ADHD-PGS: 166). DNAm signals are largely unique for individual PGSs. Finally, a DNAm-based measure of genetic susceptibility at birth nominally increased explained variance for several child cognitive and motor outcomes above PGS, but not after multiple testing correction. Interpretation. Genetic susceptibility for neurodevelopmental conditions, particularly schizophrenia, is detectable in cord blood DNAm at birth in a population-based sample, with largely distinct DNAm patterns between PGSs. These findings support the early-origins perspective on schizophrenia. Funding. HorizonEurope; European Research Council Keywords. Population-based; Genetic susceptibility; DNA methylation; Epigenetics; Neurodevelopmental conditions; Generation R Study; PREDO; ALSPAC; MoBa

In diffusion research, journalistic coverage is acknowledged as a significant factor in spreading awareness and fostering knowledge about innovation, potentially accelerating or impeding the adoption process. With regards to AI-related innovations, this dynamic has largely been studied within the context of Western developed countries. There is far less understanding of how this process unfolds in the news ecosystem of post-communist countries, particularly those with lower democratic standards and weaker economic development, such as Bosnia and Herzegovina. With the intention of gaining preliminary insights, this study investigated how the journalistic organizations in Bosnia and Herzegovina covered the emergence and societal adoption of ChatGPT, a novel form of generative AI, during the initial six-month period following its widespread availability. The content analysis of relevant news messages (N=542) published by 40 legacy and digital- only news outlets was used to explore the key characteristics of journalistic coverage, the attention given to the issue over time and the media depictions of this innovative AI technology. Results indicate that a small group of news outlets, predominantly legacy news organizations, provided significantly more content on ChatGPT than others, particularly public broadcasting services. Findings highlight a tendency among news outlets to focus on either the risks or benefits of ChatGPT and similar AI-based products and amplify sources associated with the business sector and high-tech industry, overrepresented by male voices.

Mining of the thick coal layers that include roof caving operation can results in residual coal quantities in the gob as a potential threat causing occurence of spontaneous oxidation process, smoldering, and endogenous mine fire that can affect the safety and regular mine operations. Endogeneous fire occurences in Zenica coal mines are directly linked to complex natural conditions reflecting in complex geological conditions, great depth of mining, high methane content in coal seams, and tendency of coal to spontaneous oxidation process. The subject of the paper is the analysis of endogenous fire supression method applyed in conditions of complete coal thickness longwall mining in Raspotočje mine, that has been rehabilitiated upon the endogeneous fire and then reactivated. The following methods were used in fire fighting: passive fire fighting methods (sealing of the area affected by the fire), active method (injection of electrofilter ash) and ventilation methods. Furthermore additional data (position of gob area and sealing objects, air flow regulators, routes of possible air migration, suggested technical solutions, etc) were added in the linear and canonic schemes for the purpose of defining efficient solutions for fire fighting. Key words: endogenous fire, longwall mining, advancing mining, fire fighting.

Morus alba L. is a plant with a long history of dietary and medicinal uses. We hypothesized that M. alba possesses a significant biological potential. In that sense, we aimed to generate the chemical, antimicrobial, toxicological, and molecular profile of M. alba leaf and fruit extracts. Our results showed that extracts were rich in vitamin C, phenols, and flavonoids, with quercetin and pterostilbene concentrated in the leaf, while fisetin, hesperidin, resveratrol, and luteolin were detected in fruit. Extracts exhibited antimicrobial activity against all tested bacteria, including multidrug-resistant strains. The widest inhibition zones were in Staphylococcus aureus ATCC 33591. The values of the minimum inhibitory concentration ranged from 15.62 μg/ml in Enterococcus faecalis to 500 μg/ml in several bacteria. Minimum bactericidal concentration ranged from 31.25 μg/ml to 1000 μg/ml. Extracts impacted the biofilm formation in a concentration-dependent and species-specific manner. A significant difference in the frequency of nucleoplasmic bridges between the methanolic extract of fruit (0.5 μg/ml, 1 μg/ml, 2 μg/ml), as well as for the frequency of micronuclei between ethanolic extract of leaf (2 μg/ml) and the control group was observed. Molecular docking suggested that hesperidin possesses the highest binding affinity for multidrug efflux transporter AcrB and acyl-PBP2a from MRSA, as well as for the SARS-CoV-2 Mpro. This study, by complementing previous research in this field, gives new insights that could be of great value in obtaining a more comprehensive picture of the Morus alba L. bioactive potential, chemical composition, antimicrobial and toxicological features, as well as molecular profile.

Antibiotic-resistant bacteria are currently an important public health concern posing a serious threat due to their resistance to the current arsenal of antibiotics. Uropathogens Escherichia coli (UPEC), Proteus mirabilis, Klebsiella pneumoniae and Enterococcus faecalis, antibiotic-resistant gram-negative bacteria, cause serious cases of prolonged UTIs, increasing healthcare costs and potentially even leading to the death of an affected patient. This review discusses current knowledge about the increasing resistance to currently recommended antibiotics for UTI therapy, as well as novel therapeutic options. Traditional antibiotics are still a part of the therapy guidelines for UTIs, although they are often not effective and have serious side effects. Hence, novel drugs are being developed, such as combinations of β-lactam antibiotics with cephalosporins and carbapenems. Siderophoric cephalosporins, such as cefiderocol, have shown potential in the treatment of individuals with significant gram-negative bacterial infections, as well as aminoglycosides, fluoroquinolones and tetracyclines that are also undergoing clinical trials. The use of cranberry and probiotics is another potential curative and preventive method that has shown antimicrobial and anti-inflammatory effects. However, further studies are needed to assess the efficacy and safety of probiotics containing cranberry extract for UTI prevention and treatment. An emerging novel approach for UTI treatment is the use of immuno-prophylactic vaccines, as well as different nanotechnology solutions such as nanoparticles (Nanoparticles). Nanoparticles have the potential to be used as delivery systems for drugs to specific targets. Furthermore, nanotechnology could enable the development of nano antibiotics with improved features by the application of different Nanoparticles in their structure, such as gold and copper Nanoparticles. However, further high-quality research is required for the synthesis and testing of these novel molecules, such as safety evaluation and pharmacovigilance.

The aim of this study was to analyze various environmental factors influencing dyslexia to enhance our understanding of its risk factors, including the exposure of mothers of dyslexic children to potential negative developmental influences, perinatal and postnatal developmental characteristics of dyslexic children, genetic predisposition, socioeconomic status, and reading exposure in dyslexic children.Mothers of both dyslexic and non-dyslexic children took part in the study. The home literacy environment and the development of motor skills emerge as significant risk indicators for dyslexia. These findings hold profound implications for public health, emphasizing the critical importance of early childhood in providing children with the best possible educational opportunities.Key words:risk factors, dyslexia, child development, developmental influences, home literacy environment

To maximize the impact of precision medicine approaches, it is critical to identify genetic variants underlying disease and to accurately quantify their functional effects. A gene exemplifying the challenge of variant interpretation is the von Hippel–Lindautumor suppressor (VHL). VHL encodes an E3 ubiquitin ligase that regulates the cellular response to hypoxia. Germline pathogenic variants in VHL predispose patients to tumors including clear cell renal cell carcinoma (ccRCC) and pheochromocytoma, and somatic VHL mutations are frequently observed in sporadic renal cancer. Here we optimize and apply saturation genome editing to assay nearly all possible single-nucleotide variants (SNVs) across VHL’s coding sequence. To delineate mechanisms, we quantify mRNA dosage effects and compare functional effects in isogenic cell lines. Function scores for 2,268 VHL SNVs identify a core set of pathogenic alleles driving ccRCC with perfect accuracy, inform differential risk across tumor types and reveal new mechanisms by which variants impact function. These results have immediate utility for classifying VHL variants encountered clinically and illustrate how precise functional measurements can resolve pleiotropic and dosage-dependent genotype–phenotype relationships across complete genes. Saturation genome editing characterizes von Hippel–Lindau (VHL) coding variants and their associations with diseases. Function scores for 2,268 VHL single-nucleotide variants (SNVs) classify pathogenic alleles driving renal cell carcinoma and suggest new mechanisms by which variants impact function.

This research aims to evaluate JavaScript frontend frameworks by their performance and popularity to provide practitioners with guidance in making informed decisions when choosing frontend frameworks for their projects. For that purpose, three applications with the same features were built using three best-known JavaScript frontend frameworks: Angular, React, and Vue. Comparative analysis was employed to conduct the framework comparison using the content of the three applications. For performance evaluation, tools like PageSpeed Insights, WebPageTest, GTmetrix, Pingdom, Lighthouse, and Google Performance were used. In addition, the research also offers the overall comparison of JavaScript frameworks including documentation quality, ability to develop mobile applications, learning curve, built-in features, etc. The results indicate that while React is accepted as the most popular frontend framework, Vue is the one that offers the best performance. The research also compares JavaScript frameworks by specific features they offer such as state management and analyses the implications of these features to the development process. According to the results, these features have big implications on the development process and they are very important when we are making decisions on which framework we will choose for some project.

Considering the escalating global prevalence and the huge therapeutic demand for the treatment of hypertension, there is a persistent need to identify novel target sites for vasodilator action. This study aimed to investigate the role of TRPA1 channels in carvacrol-induced vasodilation and to design novel compounds based on carvacrol structure with improved activities. In an isolated tissue bath experiment, it was shown that 1 µM of the selective TRPA1 antagonist A967079 significantly (p < 0.001) reduced vasodilation induced by 3 mM of carvacrol. A reliable 3D-QSAR model with good statistical parameters was created (R2 = 0.83; Q2 = 0.59 and Rpred2 = 0.84) using 29 TRPA1 agonists. Obtained results from this model were used for the design of novel TRPA1 activators, and to predict their activity against TRPA1. Predicted pEC50 activities of these molecules range between 4.996 to 5.235 compared to experimental pEC50 of 4.77 for carvacrol. Molecular docking studies showed that designed molecules interact with similar amino acid residues of the TRPA1 channel as carvacrol, with eight compounds showing lower binding energies. In conclusion, carvacrol-induced vasodilation is partly mediated by the activation of TRPA1 channels. Combining different in silico approaches pointed out that the molecule D27 (2-[2-(hydroxymethyl)-4-methylphenyl]acetamide) is the best candidate for further synthesis and experimental evaluation in in vitro conditions.

The tendency to abolish, to obtain heat from coal in order to reduce atmospheric pollution, leads to the closure of coal deposits. The alternative for obtaining heat, especially in the category of heating smaller residential buildings, with wood pellets, although acceptable from an ecological point of view, has its drawbacks, if one takes into account the scale of the required amount of energy and the role of the forest in purifying the air. Wood pulp and coal as basic energy carriers have hydrocarbon compounds. So, in both cases COx and NOx gases appear as gaseous products. The difference is in their scale. Unlike wood, coal also contains sulfur compounds, which makes it an environmentally unacceptable fuel. By adding a certain amount of wood pulp and additives to the coal mass, in this work, pyrophyllite shale, the impact on the energy and ecological aspects of heat release of such a complex fuel mixture was investigated. The ecological aspect is accentuated through the release of sulfur from coal. The mixture was subjected to the briquetting process, and the obtained samples were tested with relevant parameters, primarily the obtained lower heat value and sulfur content (SOx) in these products. The obtained results are very optimistic with both parameters mentioned above and can be a guide for further research for this idea. Key words: coal, wood sawdust, briquette, calorific value, sulfur, ecology.

Visual impairment can occur in different periods of life, and each person reacts differently in a way that corresponds to their personality, abilities, goals, as well as the life situation in which they find themselves. The aim of this research paper was to show, according to the available literature, the impact of individual educational-rehabilitation treatment on visual perception in students with visual impairment. The sample of respondents included a student, male, aged 9 years, diagnosed with nystagmus, amblyopia, and astigmatism. The student was included in an educational-rehabilitation program in order to improve his visual functioning. For the purpose of assessment and evaluation, the Beery-Buktenica Developmental Test of Visual-Motor Integration (VMI) fifth edition was used, which is intended for the assessment of visual-motor integration, visual perception, and motor coordination. An initial and final assessment was conducted with the student, and the Visual Perception subtest, consisting of 30 tasks, was used for the same. After the initial assessment, an individual educational-rehabilitation treatment was carried out for 2 months. The research data were processed by frequency analysis. On the basis of statistical processing and data analysis, the results showed an improvement in the area of visual perception, thereby confirming the need to implement an educational-rehabilitation program for a student with visual impairment.Keywords: visual perception,visual impairment, individual educational-rehabilitation program.

Abstract This work presents investigation of chemical composition and antioxidant activity of ethanolic extracts of leaves with flowers and berries prepared by ultrasound and Soxhlet extractions of Crataegus monogyna from Bosnia and Herzegovina. Total phenolic, flavonoid, and anthocyanin contents were measured by spectrophotometric methods. The sample of leaves with flowers extracted by Soxhlet extraction was the richest in the content of total phenolic compounds (14.43 mg GAE/g DW) and total flavonoids (2.22 mg QE/g DW). Same extract showed the best antioxidant activity with an IC50 value of 0.71 mg/mL for DPPH and 0.38 mg/mL for ABTS assay, as well as the highest content of gallic acid, caffeic acid, and hyperoside 0.04 mg GAE/g DW, 0.60 mg CA/g DW and 2.61 mg HYP/g DW, respectively, determined by HPLC-ED. Vitexin was not detected. The extract of berries obtained by ultrasound extraction had the highest amount of total anthocyanins (1.69 mg/100 g DW). Graphical Abstract