Introduction Accurate genotyping of Killer cell Immunoglobulin-like Receptor (KIR) genes plays a pivotal role in enhancing our understanding of innate immune responses, disease correlations, and the advancement of personalized medicine. However, due to the high variability of the KIR region and high level of sequence similarity among different KIR genes, the generic genotyping workflows are unable to accurately infer copy numbers and complete genotypes of individual KIR genes from next-generation sequencing data. Thus, specialized genotyping tools are needed to genotype this complex region. Methods Here, we introduce Geny, a new computational tool for precise genotyping of KIR genes. Geny utilizes available KIR allele databases and proposes a novel combination of expectation-maximization filtering schemes and integer linear programming-based combinatorial optimization models to resolve ambiguous reads, provide accurate copy number estimation, and estimate the correct allele of each copy of genes within the KIR region. Results & Discussion We evaluated Geny on a large set of simulated short-read datasets covering the known validated KIR region assemblies and a set of Illumina short-read samples sequenced from 40 validated samples from the Human Pangenome Reference Consortium collection and showed that it outperforms the existing state-of-the-art KIR genotyping tools in terms of accuracy, precision, and recall. We envision Geny becoming a valuable resource for understanding immune system response and consequently advancing the field of patient-centric medicine.

Brain natriuretic peptide (BNP) serves as a crucial cardiac biomarker in human and small animal veterinary medicine, yet its role in equine cardiology remains underexplored.This study aimed to investigate the influence of different exercise types on BNP concentrations and single brief echocardiographic parameters in two distinct horse populations, while also examining potential correlations between BNP concentrations and echocardiographic variables.Forty clinically healthy horses, categorised into working and sports groups, underwent exercise testing exercise to their purposes. BNP concentrations were measured using a horse‐specific ELISA kit, and brief echocardiographic examinations were conducted 24 h post‐exercise.Blood samples were collected before, after exercises, and at rest to determine plasma BNP concentrations. The brief echocardiographic examinations looked only at M‐mode imaging from the right parasternal window, and assessed cardiac chambers and ventricular diameters. BNP concentrations and echocardiographic variables were compared between the two groups.Exercise did not significantly increase BNP concentrations in either group, with sports horses exhibiting higher basal BNP values (18.0–1973.0 pg/mL) compared to work horses (14.4–1074.5 pg/mL). No significant differences in BNP concentrations were observed between breeds, sexes, or pregnancy statuses. Brief echocardiographic examinations using M‐mode imaging from the right parasternal window revealed significant differences in right ventricular dimensions in diastole and relative wall thickness between the groups. A low negative correlation (r = −0.329, p = 0.038) was observed between BNP concentration and fractional shortening.Single pre‐exercise blood sampling, brief M‐mode echocardiography, and lack of exercise standardisation in the endurance horse group limited assessing high biological variability on BNP concentration and echocardiographic parameters in the tested horses.Assessing BNP concentrations and echocardiographic parameters during different exercises can be helpful in examining the functional status of the equine cardiovascular system, guiding tailored exercise regimens for optimal equine well‐being.

<p>To evaluate the surface water quality of the Drina River basin, samples were collected from the main river and its tributaries at selected monitoring sites. A total of 17 samples were collected from various locations during the years 2023 and 2024. Various physicochemical parameters and concentrations of heavy metals were analyzed. Overall, most tested samples indicated generally good water quality in terms of physicochemical indicators. However, the findings also suggest that certain human activities have a measurable influence on water quality in the basin. The results serve as an indication of existing pollution pressures and provide a basis for further investigations on the environmental status of the Drina River.</p>

Cirsium (thistle) is one of the most taxonomically demanding genera within the Compositae. These taxonomic difficulties are hypothesized to result from limited morphological differentiation, incipient speciation and/or hybridization among taxa, and misinterpretations of faded and incomplete herbarium specimens. According to the latest data, the Dinarides and the Eastern Alps are inhabited by the endemic, diploid, newly described species Cirsium greimleri, which often occurs in sympatry with C. rivulare and C. erisithales. The area of the related, vicarious, tetraploid, and endemic species C. waldsteinii is limited to the southeastern Carpathians. Considering that the new knowledge refutes the existence of C. waldsteinii in Bosnia and Herzegovina, it was necessary to review all C. waldsteinii data discovered so far in Bosnia and Herzegovina. The results obtained from the distribution of the species, the predictive modelling of its ecological niche, and the analysis of the genome size confirmed the existence of the species C. greimleri in Bosnia and Herzegovina and its potential hybridization. This study indicates the need for further research into the sympatry of C. greimleri and its relatives, as well as the essential taxonomic revision of this complex.

Bitter and sweet wormwoods are traditional plant species in the Asteraceae (Compositae). Their use in traditional medicine has long been known. Numerous preparations of bitter and sweet wormwood (teas, tinctures) are used in the treatment of diseases of the digestive system. The content of bioactive components (polyphenols and flavonoids) and antioxidant activity of Artemisia absinthium L. (bitter wormwood) and Artemisia annua L. (sweet wormwood) were examined in this paper. Extract's series were prepared by mixing selected organic solvents (methanol, ethanol and acetone) and water in different volume ratios for both analyzed species. Antioxidant activity was tested using FRAP and DPPH methods. Extracts of sweet wormwood contain more bioactive components and have a higher antioxidant capacity compared to extracts of bitter wormwood. In terms of extraction efficiency, the mixture of acetone and water (20:30 v/v) proved to be the most efficient. Regarding pure organic solvents, the most effective for the bioactive components isolation is ethanol, while acetone showed the weakest extraction power.

Two highly invasive plant species, Lysimachia vulgaris and Lythrum salicaria are well-known for its anti-inflammatory, hemostatic, and antidiaroic activity. These plants are most widespread on the territory of Euroasia, where their traditional medicinal use has been reported. Due to their invasiveness, they are undesirable plants in  other parts of the world. Till this day, many studies were conducted regarding the biological and pharmacological activity of L. salicaria. In this study, the polyphenol content of plant extracts was examined using the Folin-Ciocalteou method. Antioxidant activity of selected plant species was also determined, using DPPH and FRAP methods. Extracts of different polarities were prepared using methanol, water, and acetone. Extraction was performed by maceration and ultrasonic extraction. The results of the study show that both plant species possess antioxidant activity. Lythrum salicaria extracts show a significant polyphenol content and antioxidant capacity, with results notably higher than the results of studies conducted so far. The different antioxidant activity of the prepared extracts confirms the influence of solvents and extraction methods on the utilization of the antioxidant potential of plants. Additionally, for the aqueous extracts prepared by ultrasonic extraction method, an in vitro study of antibacterial activity was conducted. Both plant species show antibacterial activity, with an emphasis on the very strong antibacterial activity of L. salicaria extracts against selected bacterial strains.

Introduction: The early adolescence period represents a phase of turbulent physical development, characterized by disproportion in the musculoskeletal system, where the muscular system's inability to maintain a normal relationship between certain body segments results in poor posture among adolescents. Back pain is an increasing health problem among children and adolescents. Postural deviations and back pain are common issues among children, caused by the long-term effects of various risk factors associated with modern lifestyles. Methods: This study is a non-experimental (qualitative) research, specifically a scientific literature review. For the purposes of this paper, a search of relevant databases including Web of Science, Scopus, PubMed, and Med-Line was conducted for articles published between 2011 and 2024. Results: A significant number of articles were published between 2011 and 2024 in relevant scientific databases. After removing duplicates and irrelevant studies, 13 studies were included in the research. The studies used in this paper were published in Poland, the Czech Republic, Portugal, Tunisia, China, Egypt, Turkey, Pakistan, Brazil, Lebanon, Bosnia and Herzegovina, and Slovenia. Conclusion: Based on the review of scientific literature, it can be concluded that early identification of risk factors is essential, as it will reduce the frequency of postural deviations and back pain in children during early adolescence. Timely implementation of educational preventive programs on exercises, education about proper posture, sitting techniques, correct school bag carrying, the use of ergonomically adjusted school furniture, and promoting an active lifestyle at home and school, along with regular physical activity, is necessary.

The paper deals with the analysis of the fuel consumption of skidders during timber extraction from thinning of even-aged beech forest on mountain terrain. Fuel consumption research was conducted on the Ecotrac 140V cable skidder over 8 working days at the same worksite during real timber extraction work. The worksite was organized so that the empty skidder traveled uphill, and when loaded, it moved downhill. The skidder was equipped with measuring devices for collecting data from sensors, the motor, and data transfer. The key parameters measured include total fuel consumption (mL) and skidder GPS position, while slopes of skid trails and load volumes were measured directly on terrain. Fuel consumption (L, L/m3) was determined per work cycle and work cycle elements. The highest fuel consumption occurred while driving the unloaded skidder, accounting for 38% of the total. This is primarily because fuel usage during skidder movement is significantly affected by factors such as skidding distance, slope, and skid trail conditions, especially since the unloaded skidder was moving uphill. Guidelines for better and more efficient organization of work and reduction in fuel costs are presented, and the suitability of the skidder and harvesting system are considered based on the results of fuel consumption.

Derviš Sušić is undoubtedly one of the most versatile writers of the 20th century Bosnia and Herzegovina. His opus, characterised by a peculiar stylistic expression contains metaphors that make the storyline, otherwise set in a certain historical period, timeless, i.e., ever-current and again-enlightening. This paper aims at analysing metaphors in the translation of Sušić’s novel Uhode, published in English in 2017 by the Academy of Arts and Sciences of Bosnia and Herzegovina as Spies. The methodological framework applied is Lakoff and Johnson’s linguistic approach, whereby the source text and the translation are compared within their source and target domains. In that sense, translatability, i.e. the possibility of achieving the identical content of the source and target domains in the translation is observed within Newmark’s model of translation strategies.

The paper provides a brief analysis of the crime of genocide through historical and legal contexts, emphasizing that the twentieth century is often referred to as the “century of genocide” due to the numerous mass atrocities committed. The paper relies on the legal definition of genocide and the efforts of Raphael Lemkin that led to the adoption of the 1948 Convention on the Prevention and Punishment of the Crime of Genocide. Among other things, we explore the complexity of proving genocidal intent, examine the role of ideology in the execution of genocide, and highlight the challenges in classifying crimes as genocide. Despite international efforts to prevent and punish perpetrators of genocide, crimes with elements of genocide continued throughout the 20th and 21st centuries, as illustrated by the example of the genocide in Bosnia and Herzegovina. The paper pays special attention to the chronology of the genocide in and around Srebrenica in July 1995 as an example of genocidal intent and execution. It also points out the ongoing denial of the adjudicated genocide in Srebrenica and its consequences for Bosnian society and the state.

Traditional Diffusion Tensor Imaging (DTI) metrics are affected by crossing fibers and lesions. Most of the previous tractometry works use the single diffusion tensor, which leads to limited sensitivity and challenging interpretation of the results in crossing fiber regions. In this work, we propose a tractometry pipeline that combines white matter tractography with multi-tensor fixel-based metrics. These multi-tensors are estimated using the stable, accurate and robust to noise Multi-Resolution Discrete Search method (MRDS). The spatial coherence of the multi-tensor field estimated with MRDS, which includes up to three anisotropic and one isotropic tensors, is tractography-regularized using the Track Orientation Density Imaging method. Our end-to-end tractometry pipeline goes from raw data to track-specific multi-tensor-metrics tract profiles that are robust to noise and crossing fibers. A comprehensive evaluation conducted in a phantom simulating healthy and damaged tissue with the standard model, as well as in a healthy cohort of 20 individuals scanned along 5 time points, demonstrates the advantages of using multi-tensor metrics over traditional single-tensor metrics in tractometry. Qualitative assessment in a cohort of patients with relapsing-remitting multiple sclerosis reveals that the pipeline effectively detects white matter anomalies in the presence of crossing fibers and lesions.

Educating teachers to teach language, culture and history in a post-conflict country such as Bosnia and Herzegovina, which is still deeply divided and fragmented, implies enormous social and moral responsibilities. These endeavours represent continuous challenges where the processes of healing the wounds in such vulnerable situations, so deep and irreparable, are discussed and contextualised within the long-term social recovery in which the education of children and young people takes on a primarytransformative role. The present paper examines the education policies and divisions in Bosnia and Herzegovina in the past twenty years, which reflect the segregation, politicisation and fragmentation in the post-war society. It also focuses on the role of educators in teaching for peace, antidiscriminationand intercultural understanding by addressing the issues of social exclusion, injustice, prejudice, privilege and violence across the curriculum. It problematises the construction and representation of historicaland cultural knowledge, which is usually ethnocentric in orientation. Special emphasis is placed on the role of universities, i.e., university teachers and teacher educators, in promoting critical thinking and universal humanistic values among students in Bosnia and Herzegovina in general, and those studying at the English Department, University of Sarajevo, in particular. Drawing on the theories and resources of critical and intercultural pedagogy and peace education, the paper explores the possibilities of discussing stereotypes and prejudice with first-year BA students at the English Department within their Introduction to BritishStudies course, and with MA student teachers within their Interculturalism in Language Education course, which deals with peacebuilding and intercultural sensitivity in teaching English from a cultural perspective.

This study presents a comparative analysis of titanium leaching from tionite (a byproduct of the titanium dioxide production process) and carbothermally reduced red mud (derived from aluminum residues). Tionites from the sulfate process and red mud residue are known for their environmental impacts due to their metal content and acidic/basic nature. This study explored leaching as a method to recover titanium and other metals under high-pressure and high-temperature conditions using sulfuric acid. Experiments were conducted in an autoclave with different parameter changes, like varying oxygen pressure, temperature, and reaction time to optimize metal extraction. The leaching efficiency of titanium was found to be higher in the carbothermal-reduced slag compared to tionite due to the altered mineral phases in the reduced material. XRD and SEM-EDS analyses confirmed the differing leaching behaviors, with titanium compounds in tionite showing greater resistance to dissolution. These findings highlight the importance of thermal pre-treatment for optimizing metal recovery from industrial residues. The main aim of this study is to contribute to the development of sustainable waste management solutions for tionites and red mud, emphasizing the potential of hydrometallurgical methods for metal recovery. The results are expected to inform future research and industrial applications, advancing the recovery of valuable metals while reducing the environmental footprint of titanium and aluminum residue disposal.

Pancreatic steatosis and metabolic-dysfunction-associated steatotic liver disease are characterised by fat accumulation in abdominal organs, but their correlation remains inconclusive. Recently proposed deep learning (DL) for proton density fat fraction (PDFF) estimation, which quantifies organ fat, has primarily been assessed for quantifying liver fat. This study aims to validate DL models for pancreatic PDFF quantification and compare pancreas and liver fat content. We evaluated three DL models—Non-Linear Variables Neural Network (NLV-Net), U-Net, and Multi-Decoder Water-Fat separation Network—against a reference PDFF measured using a graph-cut-based method. NLV-Net showed a strong correlation (Spearman rho) with the reference PDFF in the six-echo pancreatic head (slope: 1.02, rho: 0.95) and body (slope: 1.04, rho: 0.94) and a moderate correlation in the three-echo pancreatic head (slope: 0.44, rho: 0.40) and body (slope: 0.49, rho: 0.34). Weak correlations were found between liver and pancreatic body PDFF using graph cut in six-echo (slope: −0.041, rho: −0.12) and three-echo images (slope: 0.0014, rho: 0.073) and using NLV-Net in six-echo (slope: −0.053, rho: −0.12) and three-echo images (slope: −0.014, rho: −0.033). In conclusion, NLV-Net showed the best agreement with the reference for pancreatic fat quantification, and no correlation was found between liver and pancreas fat.

U prvom dijelu teksta ukratko je skrenuta pozornost na fenomen pasa kao kućnih ljubimaca s jedne, te pasa lutalica s druge strane. Osim toga, navedeni su određeni primjeri intenzivnog suodnosa čovjeka i psa, kako kroz historiju, jednako tako i u epohi postkulture. Posebno je razviđan bioetički problem odnosa ljudi prema psima lutalicama u Bosni i Hercegovini pri čemu je podcrtana etičko-moralna (ne)odgovornost nekadašnjih vlasnika pasa. Tijekom reflektiranja navedenih sadržaja, osim u bioetičkom rakursu, ova tematika je promišljana i iz rakursa muslimanske Tradicije, odnosno teologije morala. Tom prigodom pojam psa je sagledavan dominantno iz muslimanske teološko-moralne perspektive, ali u dijalogu sa bioetikom, tj. kroz prizmu integrativne bioetike.