Introduction. The anterior and middle superior alveolar (AMSA) nerve block is an alternative technique of local anesthesia in the maxilla, unpredictably efficient for pulpal anesthesia. The aim of this study was to determine the anesthetic efficacy of the AMSA injection for pulpal anesthesia, using computer-controlled injection system or conventional syringe, and two local anesthetic solutions with or without adrenaline. Methods. The authors administered two AMSA injections during two separate appointments, utilizing the computer-controlled system and conventional syringe to 40 subjects, divided into two groups of 20 subjects each depending on the local anesthetic used. A pulp tester was used to test the achieved anesthesia of the central and lateral incisors, canine, first and second premolars, and the first molar in 10-minute cycles over a period of 60 minutes. Duration of anesthesia for all the mentioned teeth was also determined for both the anesthetic solutions and ways of application. Results. The AMSA injection with both types of equipment was successful, showing slow onset, satisfying intensity, and declining duration of pulpal anesthesia at the last two measurements. Local anesthetic with vasoconstrictor exhibited a significantly longer pulpal anesthesia. Conclusion. The AMSA nerve block could be recommended for achieving pulpal anesthesia of maxillary teeth from the region of the first incisor to the second premolar.

Introduction. Acute otitis media is a very common disease in the early childhood age, with typical symptoms such as otalgia and fever. Otogenic complications are divided into extracranial and intracranial. Although the introduction of antibiotics has significantly reduced the incidence of intracranial complications, they are still present. Clinical picture usually develops fast, with the predominance of neurologic symptoms. Case outline. An 11-year-old boy was admitted to a tertiary health care children?s hospital because of fever, agitation, altered behavior and disorder of consciousness. Based on the anamnesis, clinical examination, CT, MRI, and lumbar puncture, it has been established that it is a case of meningoencephalitis as complications of acute otitis media. Besides intense antibiotic and symptomatic therapy, surgical treatment too was conducted as well. Firstly, mastoidectomy with the implantation of ventilation tube was done, followed by radical tympanomastoidectomy, because there was no improvement. The treatment was followed by numerous complications, such as toxic hepatitis, mycoplasma pneumoniae infection, and hemolytic anemia. The treatment lasted for 71 days, and the patient was discharged from the hospital in a good general condition, without the focal motor failure. Conclusion. Meningoencephalitis is unusual and rare complication of acute otitis media that requires urgent diagnostic procedure and multidisciplinary approach to the treatment. Surgical treatment of the ear that caused complications should not be postponed, and the choice of surgical method must be adapted to each patient individually. Hospital treatment of these patients is often prolonged and auditory and neurological sequelae are substantial and require long-term treatment.

In the present study, we investigated the antiproliferative activity of essential oil from leaves of Melissa officinalis L. grown in Southern Bosnia and Herzegovina. In vitro evaluation of antiproliferative activity of the M. officinalis essential oil was carried out on three human tumor cell lines: MCF-7, NCI-H460 and MOLT-4 by MTT assay. M. officinalis essential oil was characterized by high percentage of monoterpenes (77,5%), followed by the sesquiterpene fraction (14,5%) and aliphatic compounds (2,2%). The main constituents of the essential oil of M. officinalis are citral (47,2%), caryophyllene oxide (10,2%), citronellal (5,4%), geraniol (6,6%), geranyl acetate (4,1%) and βcaryophyllene (3,8%). The essential oil showed significant antiproliferative activity against three cancer cell lines, MOLT-4, MCF-7, and NCI-H460 cells, with GI50 values of <5, 6±2 and 31±17 μg/mL, respectively. The results revealed that M. officinalis L. essential oil has a potential as anticancer therapeutic agent.

In multi-domain product development organizations, there is a continuous need to transfer captured knowledge between engineers to enable better design decisions in the future. The objective of this paper is to evaluate how engineering knowledge can be captured, disseminated and (re)used by applying a knowledge reuse tool entitled Engineering Checksheet (ECS). The tool was introduced in 2012 and this evaluation has been performed over the 2017–2018 period. This case study focused on codified knowledge in incremental product development with a high reuse potential both in and over time. The evaluation draws conclusions from the perspectives of the knowledge workers (the engineers), knowledge owners and knowledge managers. The study concludes that the ECS has been found to be valuable in enabling a timely understanding of technological concepts related to low level engineering tasks in the product development process. Hence, this enables knowledge flow and, in particular, reuse among inexperienced engineers, as well as providing quick and accurate quality control for experienced engineers. The findings regarding knowledge ownership and management relate to the need for clearly defining a knowledge owner structure in which communities of practice take responsibility for empowering engineers to use ECS and as knowledge evolves managing updates to the ECS.