Cilj. Studija je sprovedena da utvrdi navike u ishrani kod zdravstvenih i prosvjetnih radnika u radi bližeg uvida u nutritivni obrazac kod ovih profesija, što bi unapredilo teorijska znanja u ovoj oblasti i planiranje i sprovođenje preventivnih mera u ciljnim populacionim grupama. Metode. Svi zdravstveni radnici zaposleni u Domu zdravlja ,,Dr Nika Labović”u Beranama i svi zaposleni prosvjetni radnici u Osnovnoj školi ,,Vuk Kardžić“ u Beranama su popunili anketu o ishrani. Podaci su prikupljeni prospektivno i analizirani metodama deskriptivne statistike i testiranja hipoteze. Rezultati. Ukupno je bilo 180 odraslih ispitanika oba pola (109 zdravstvenih i 71 prosvetni radnik), u životnom dobu od 43.70±11.90 (srednja vrednost±standardna devijacija) u grupi zdravstvenih radnika i 42.29±10.90 godine u grupi prosvetnih radnika. Nije bilo statistički značajne razlike između grupa u vrsti masnoća za pripremanje hrane, učestalost pripremanje hrane kod kuće, prosječnom broj kašika šećera za šolju kafe ili čaja, dodavanje soli hrani tokom obroka i upotrebi mlijeka. Zdravstveni radnici su značajno češće imali jutarnji obrok (79 vs 42, χ2=3.444, p=0.045), veći broj šolja kafe dnevno (3.17±1.23 vs 2.67±0.98, F=7.330, p=0.008) a manje prosječan broj kriški hljeba koji nije ražani i bjeli na dnevnom niovu (2.00±1.38 vs 3.06±1.53, F=5.292, p=0.027).

In the present study modern technology of DNA extraction and automatic genotyping was applied in Bosnian and Herzegovinian autochthonous horse breed by using 17-Plex horse genotyping kit. The study was aimed at investigating usefulness of the 17-plex STR Kit for Bosnian mountain horse genotyping and establishing highly useful microsatellite markers system for genetic diversity studies in Bosnian mountain horse breed. Genomic DNA was extracted from whole blood collected from 22 unrelated Bosnian mountain horse specimens. A total of 95 alleles were detected. Average number of detected alleles per locus was 5.588, varying from 3 (HTG7) to 10 (ASB17). Average effective number of alleles was 3.603, fluctuating from 1.789 (HMS7) to 5.728 (HMS2). The observed heterozygosity ranged from 0.136 (HMS3) to 0.909 (ASB2) with a mean of 0.631. The results indicate that the studied population originates from the appropriate number of parent generations. The mean expected heterozygosity was 0.690, varying from 0.441 (HMS7) to 0.853 (ASB17) indicating high genetic variability within Bosnian mountain horse population. The PIC values ranged from 0.409 (HMS7) to 0.837 (ASB17) with a mean of 0.643, suggesting that 94.12% markers were quite informative in terms of their suitability for genetic diversity studies .The most polymorphic locus was HMS2 and the least polymorphic locus was HMS7. The inbreeding coefficient ranged from -0.030 (HMS7) to 0.807 (HMS3) with a mean of 0.077. Inbreeding coefficient values indicated no shortage of heterozygotes in Bosnian mountain horses. Deviation from Hardy-Weinberg equilibrium (p<0,05) was found in three loci (HTG10, HMS3 and ASB17). The applied set of 17 microsatellite markers proved to be sufficiently specific for use in genotyping of Bosnian mountain horse. Considering the values of HO, HEand PIC over 0.6, five microsatellite markers system (HTG4, AHT4, AHT5, ASB2, HMS2) is considered to be highly useful for genetic diversity studies in Bosnian mountain horse breed.

Microsatellites (MS) have been, for the last two decades, widely used for parentage analysis in all types of livestock, including pigs. Conservation efforts on Banija spotted pigs included genetic characterization of the breed with MS markers. Since recent comparison of pedigree and MS data revealed some inconsistencies, the aim of this study was to develop a set of highly polymorphic and heterozygous MS markers, which could be used for parentage analysis and to prevent pedigree errors. 12 MS markers with the polymorphic information content (PIC) above 0.62 were chosen and combined into a single multiplex polymerase chain reaction (PCR). The combined non-exclusion probability for one candidate parent (NE-1P) was 0.00246149, the combined NE-1P given the genotype of a known parent of the opposite sex (NE-2P) was 0.00003739 and combined non-exclusion probability for a candidate parent pair (NE-PP) was 0.00000003. Due to a high information content of selected MS markers it was possible to obtain high accuracy in parentage assignment, which was confirmed by analyzing actual data with known genetic relationships