The large body of published literature has shown that the effects of strength training can transfer from trained to untrained homologous limb muscles after unilateral training. These effects on strength have been shown to be very specific to the type and speed of training contraction. The aim of this study was to investigate the effects of a 4-week unilateral slow and fast velocity isokinetic concentric training, to compare the effects, and thus investigate whether these effects are speed-specific. Forty-four healthy female students allocated to slow training, fast training, or control performed 12 isokinetic concentric-concentric plantar/dorsal flexors training sessions (3 × 4 weeks) using their nondominant leg. Participants in the two experimental groups showed statistically significant gains in strength in both the trained (ranging from 8 to 41%) and untrained leg (5–26%), thus showing cross-education on strength effects. The present study demonstrated that 4 weeks (12 training sessions) of unilateral isokinetic resistance training in the concentric mode improved the strength of contralateral, untrained homologous muscles to the same extent, regardless of the contraction velocity used in females. Furthermore, the amount of concentric overload (50% more than during 60°/s) did not appear to affect the increase in strength gains. Therefore, practitioners are encouraged to use both training speeds when strength gains in the contralateral leg are the primary goal. If the training time is limited, however, training with a higher contraction speed is recommended.

Assessing the consistency of quantitative MRI measurements is critical for inclusion in longitudinal studies and clinical trials. Intraclass coefficient correlation and coefficient of variation were used to evaluate the different consistency aspects of diffusion‐ and myelin‐based MRI measures. Multi‐shell diffusion and inhomogeneous magnetization transfer data sets were collected from 20 healthy adults at a high‐frequency of five MRI sessions. The consistency was evaluated across whole bundles and the track‐profile along the bundles. The impact of the fiber populations on the consistency was also evaluated using the number of fiber orientations map. For whole and profile bundles, moderate to high reliability of diffusion and myelin measures were observed. We report higher reliability of measures for multiple fiber populations than single. The overall portrait of the most consistent measurements and bundles drawn from a wide range of MRI techniques presented here will be particularly useful for identifying reliable biomarkers capable of detecting, monitoring and predicting white matter changes in clinical applications and has the potential to inform patient‐specific treatment strategies.

Plants are sessile organisms that are under the constant influence of the environmental conditions in which they grow. Any change in "inanimate" factors that have a significant impact on plant growth and development is collectively referred to as "abiotic stress". Extreme temperatures, especially in changed climatic conditions, are one of the most harmful abiotic factors, which cause heat stress in plants. The effects of high temperatures can be manifested through various morphological, physiological and genetic changes in the plant organism. Generally speaking, plants can be divided into three groups according to temperature requirements: psychrophilic plants, mesophilic plants and thermophilic plants. Most woody species belong to the group of mesophilic plants, which require temperatures between 10 and 30 °C for successful growth and development. As the temperature changes on a daily and seasonal basis in relation to the optimal range, certain changes occur in the plant organism that are necessary to maintain cell growth and homeostasis. Regardless of their ability to adapt to temperature oscillations, plants that have been exposed to temperatures above the optimal level for a long time can show symptoms of irreversible damage, which manifest as heat stress. Generally speaking, this type of stress is considered to occur when the temperature is 10- 15 °C higher than usual at some time of the year, and lasts long enough to cause irreversible damage or disturbances in plant growth and development. As the intensity, duration and degree of temperature change change, so do the various effects of heat stress on plants. In order to survive, plants must adapt to changes in environmental conditions through a specific response that depends on the physiology and morphology of a species. According to their level of tolerance to high temperatures, plants can be divided into three categories: heat-sensitive, relatively heat-sensitive, and heattolerant. The shape and strength of tolerance to high temperatures mostly depend on the plant species, tissue type and cells that are exposed to negative influences. The increase in ambient temperature can also jeopardize the productivity of agricultural crops and forest trees, which has been visible in recent decades in light of climate change and projections regarding the security of supply of the growing population on Earth. Injuries resulting from high temperatures during a fire can initiate a cascade of complex mechanisms that affect the physiology of trees after a fire. The discovery of exact physiological mechanisms and corresponding specific injuries that occur on individual trees, as well as in forest ecosystems, are the focus of intensive modern research. Recent studies have made critical strides in understanding the physiological processes in trees that manifest after fire injuries, and these injuries can affect the tree in combination with some other stressful conditions, such as drought and insect and pathogen attacks. The paper presents a conceptual framework that combines all these processes, their mutual interactions and possible responses, and puts these plant responses in the context of existing hypotheses about the impacts of specific disturbances on plants and ecosystems. By focusing on carbon and water as the main factors in the functioning of the plant organism, this paper presents cambium/phloem necrosis and xylem damage as the main effects of fire injuries. The resulting lack of carbon and hydraulic dysfunction of plants are associated with drought and insect attack. Assessing the precise relationships of the processes presented will be crucial to fully understanding how fires can affect tree functionality and will help improve fire risk assessment and predict tree mortality models. Knowledge of the physiological responses of trees is important for a better assessment of ecosystem dynamics after a fire and their interaction with climate disturbances, and especially taking into account the predicted increase in the frequency and intensity of fires.

The world’s leading health institutions warn that adolescents do not meet the minimum level of physical activity necessary for the proper physical development of young people, which is considered one of the main problems of public health. In addition to personal preference, environmental and cultural factors play an important role in the involvement of young people in sports. This study aimed to examine potential differences in involvement in sports, anthropometric characteristics and motor abilities, in adolescents of different residential statuses. The sample included 83 adolescents, of whom 45 (54.2%, 13.51±0.55 yrs) were from urban areas and 38 (45%, 13.45±0.50 yrs) were from rural areas in the Belgrade region. Surveying detected their involvement in sports, and anthropometric characteristics were assessed through body height (BH) body mass (BM), body mass index (BMI), and motor abilities through explosive strength of lower and upper extremities and agility. A Mann-Whitney U test has shown that place of residency impacts involvement in sports and motor abilities, while there was no impact on anthropometric characteristics. Cohen’s criteria (r) detected the level of impact. Adolescents from urban areas are more involved at sports and have better motor abilities. There is no difference in anthropometric characteristics. Considering the relatively small sample of respondents and variables assessed, the obtained results can hardly be generalized. However, this does not diminish the importance of the present study which examined the urban-rural state of sports activity, anthropometric characteristics and motor abilities of adolescents in Serbia, thus making an important contribution to this field.

A growing issue on a global scale is the emergence of helminth species and populations that are resistant to one or more anthelmintics. The majority of currently available anthelmintics used to control parasitic nematodes of cattle and sheep belong to only three main groups, benzimidazoles, imidazothiazoles and avermectins/milbemycins. The availability of reliable and precise techniques for its identification and monitoring is a critical component of the success of helminth control programs intended to prevent the spread of resistance in nematode populations. In vivo method like fecal egg count reduction test and in vitro methods such as egg hatch assays, larval motility test, larval development test and polymerase chain reaction (PCR) can be used for the detection of anthelmintic resistance although each has some reliability, repeatability, sensitivity, and ease of interpretation issues. The genetic basis of resistance to the majority of anthelmintics are still not well understood. Thanks to recent developments in high-throughput sequencing, it is now possible to define features such as drug resistance using genome-wide techniques. Keywords: Anthelmintics; Helminths; Resistance; Detection assays; Molecular diagnostics; Parasite control

In this study, using the methods of spatial autocorrelation in geographic information systems (GIS), an analysis of the spatial distribution of mortality rates in Bosnia and Herzegovina was performed in order to identify high-risk areas with increased mortality and depopulation. Spatial analysis is based on the calculation of global (Global Moran’s I and Getis-Ord General G) and local (Anselin Local Moran’s I and Getis-Ord Gi*) statistical indices of spatial autocorrelation. The values of global statistical indices confirmed a clustering of high mortality values, while local statistical indices confirmed high mortality rates in the municipalities of northwestern and eastern Bosnia and Herzegovina. Conducted research provides an insight into spatial patterns of demographic processes, while results obtained by this research are significant for demographic development and future spatial demographic research in Bosnia and Herzegovina.

This study aimed to explore the use of nominal and verbal Anglicisms by Bosnian high school students, the students’ familiarity with these Anglicisms and their attitudes towards them and it also aimed to see whether these factors vary with respect to the frequency of students’ use of English in speaking, listening, reading and writing. The findings point to a very frequent use of Anglicisms among Bosnian participants, particularly verbal Anglicisms which tended to be significantly more frequently employed than nominal Anglicisms, and slightly greater familiarity with the English form rather than with the Bosnian equivalent form. Moreover, the frequency of the use of Anglicisms was related to the frequency of reading and writing in English, while the familiarity with the Bosnian equivalent was significantly related to speaking and reading and familiarity with the English equivalent form to all four aspects of English language use. The participants also expressed rather positive attitudes towards Anglicisms, perceiving them as a useful addition and not a serious threat to their native language.

Chronic myeloid leukemia (CML) is a hematological disorder characterized by increased proliferation of the granulocytic cell lineage. We diagnose CML by presence of the breakpoint cluster region-abelson (BCR-ABL1) oncogene using the quantitative real-time polymerase chain reaction (QRT-PCR). This method provides an accurate and unambiguous follow-up of the treatment response to tyrosine kinase inhibitors (TKIs). This study aimed to determine the molecular response to the first and second generation of TKIs in first and second line of treatment using the QRT-PCR method. We conducted a retrospective study on 48 CML patients treated with the first and second generation of TKIs in first and second-line treatment. Treatment responses have been followed-up every 3 months using the QRT-PCR method. Patients were divided into three groups according to molecular responses to the first line of TKIs. Results obtained in this study showed that the first group of patients did not achieve major molecular response (MMR) in the first 18 months of TKI treatment. The second and third group of patients achieved MMR and deep molecular response (DMR) in the first 18 months of TKI treatment. These results indicate that patients with MMR and DMR in the first 18 months of TKIs treatment had a favourable clinical course of the disease. Inadequate molecular responses to the first line of TKIs can be improved with in increase of the dose of TKIs or by switching to other TKIs. Continuous and timely molecular monitoring of TKI’s response in CML patients provides a careful observation of the disease's course and a proper treatment approach.

Background Understanding the anatomy and morphological variability of the orbital region is of great importance in clinical practice, forensic medicine, and biological anthropology. Several methods are used to estimate sex based on the skeleton or parts of the skeleton: classic methods and the geometric morphometric method. The objective of this research was to analyse sex estimation of the orbital region on a sample of skulls from a Bosnian population using the geometric morphometric method. Materials and Methods The research was conducted on three-dimensional models of 211 human adult skulls (139 males and 72 females) from the Osteological Collection at the Faculty of Medicine in Sarajevo. The skulls were recorded using a laser scanner to obtain skull 3D models. We marked 12 landmarks on each model to analyse sexual dimorphism. Landmarks were marked using the program Landmark Editor. After marking the landmarks, we used the MorphoJ program to analyse the morphological variability between male and female orbital regions. Results After Procrustes superimposition, generating a covariant matrix, and introducing sex as a variable for classification, a discriminant functional analysis (DFA) was applied which determined the estimation for males with 86.33% accuracy and for females with 88.89% based on the form of the orbital region. The results of regression analysis showed that the size of the orbital region has a statistically significant effect on its shape's sexual dimorphism. After excluding the influence of size and providing DFA, we concluded that sex estimation was possible with 82.01% accuracy for males and 80.55% accuracy for females based on the shape of the orbital region in the examined sample. Conclusion Sex estimation based on the orbital region was possible with more than 80% accuracy for both sexes, which is a high percentage of correct estimation. Therefore, we recommend using the orbital region of the skull for sex estimation.

The objective of this paper was to present basic clinical characteristics and outcomes of treating Covid 19 patients during the second wave of the pandemic. In the retrospective study for the period from September 2020 to February 2021 it was analyzed disease history data and radiological lung changes, time from the initial start of the disease until hospitalization, parameters of blood gas analysis, comorbidities, and the outcome. The research covered 409 patients, out of which 263 (64.3%) were males. Average age was 67.07± 12.44 years (min. 20; max. 93). A high comorbidities prevalence (82.9%) was noticed out of which arterial hypertension (69.2%), diabetes mellitus (37.7%) and obesity (24.7%). On the radiological lung scan the most noticed changes were consolidation (46.2%), “ground glass” (41.3%) and interstitial changes (13%). Bilateral lung infiltration was noticed in 91.9% of the patients. Average oxygen saturation was 84.29%±10.28% (min. 35; max. 98; med. 87%). In patients with unilateral lung infiltration, average oxygen saturation was 85.09%±8.60% (med. 89%, min.61% max 98%), while in patients with bilateral lung infiltration average was 84.22%±10.42% (med. 87%, min. 35%, max. 98%). From the total all patients’ death was noticed in 35.7% cases. Morbidity of patients with unilateral lung infiltration was 27.3% and in patients with unilateral infiltration 36.4%. Hospital admission in the first week of the disease indicates the severity of the clinical condition and can be a predictor of poor outcome. Bilateral pulmonary infiltration, obesity and diabetes mellitus are risk factors for high mortality.

Inflammation in the central nervous system (CNS) can impair the function of neuronal mitochondria and contributes to axon degeneration in the common neuroinflammatory disease multiple sclerosis (MS). Here we combine cell type-specific mitochondrial proteomics with in vivo biosensor imaging to dissect how inflammation alters the molecular composition and functional capacity of neuronal mitochondria. We show that neuroinflammatory lesions in the mouse spinal cord cause widespread and persisting axonal ATP deficiency, which precedes mitochondrial oxidation and calcium overload. This axonal energy deficiency is associated with impaired electron transport chain function, but also an upstream imbalance of tricarboxylic acid (TCA) cycle enzymes, with several, including key rate-limiting, enzymes being depleted in neuronal mitochondria in experimental models and in MS lesions. Notably, viral overexpression of individual TCA enzymes can ameliorate the axonal energy deficits in neuroinflammatory lesions, suggesting that TCA cycle dysfunction in MS may be amendable to therapy.