Cross-kingdom RNA interference (ckRNAi), the bidirectional communication between microbes and their host counterparts, is a key element in the outcome of host colonization. Whether mutualistic fungi of the Serendipitaceae family with their broad host range use small RNA effectors (sRNAs) to colonize plant roots is still under debate. To investigate if ckRNAi is a factor in the symbiosis of Serendipita indica (Si) with Arabidopsis thaliana (Ath), we established a pipeline to validate expression, translocation and post-transcriptional gene silencing of host genes by Si-derived sRNAs (SisRNAs). First, we confirmed the expression of SisRNAs both in axenic fungal culture and during Ath root colonization using stem-loop PCR. Then, to verify the translocation of putative SisRNA effectors, an ARGONAUTE 1 immuno-purification assay (AtAGO1-IP) was employed, detecting fungal SisRNAs being loaded into the plant RNAi machinery in Si-colonised roots. Subsequently, SisRNAs and artificial sRNAs (amiRNAs), were transiently expressed in Ath protoplasts to test their gene silencing activity. Stem-loop PCR confirmed expression of sRNA effectors and qPCR validated post-transcriptional gene silencing of their predicted target genes involved in cell wall organization, hormonal signalling regulation, plant immunity and gene expression. Moreover, 5’-RLM-RACE analysis revealed amiRNA-mediated canonical cleavage in Arabidopsis targets. In conclusion, this study provides a blueprint for rapid selection and analysis of sRNA effectors in plant-microbe interactions in general and suggests cross-kingdom communication in the Sebacinoid symbiosis.

Therapeutic approaches targeting proteins on the surface of cancer cells have emerged as an important strategy for precision oncology. To fully capitalize on the potential impact of drugs targeting surface proteins, detailed knowledge about the expression patterns of the target proteins in tumor tissues is required. In castration-resistant prostate cancer (CRPC), agents targeting prostate-specific membrane antigen (PSMA) have demonstrated clinical activity. However, PSMA expression is lost in a significant number of CRPC tumors, and the identification of additional cell surface targets is necessary in order to develop new therapeutic approaches. Here, we performed a comprehensive analysis of the expression and co-expression patterns of trophoblast cell-surface antigen 2 (TROP2), delta-like ligand 3 (DLL3), and carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) in CRPC samples from a rapid autopsy cohort. We show that DLL3 and CEACAM5 exhibit the highest expression in neuroendocrine prostate cancer (NEPC), while TROP2 is expressed across different CRPC molecular subtypes, except for NEPC. We observed variable intra-tumoral and inter-tumoral heterogeneity and no dominant metastatic site predilections for TROP2, DLL3, and CEACAM5. We further show that AR amplifications were associated with higher expression of PSMA and TROP2 but lower DLL3 and CEACAM5 levels. Conversely, PSMA and TROP2 expression was lower in RB1-altered tumors. In addition to genomic alterations, we demonstrate a tight correlation between epigenetic states, particularly histone H3 lysine 27 methylation (H3K27me3) at the transcriptional start site and gene body of TACSTD2 (encoding TROP2), DLL3, and CEACAM5, and their respective protein expression in CRPC patient-derived xenografts. Collectively, these findings provide novel insights into the patterns and determinants of expression of TROP2, DLL3, and CEACAM5 with important implications for the clinical development of cell surface targeting agents in CRPC.

Abstract:  In informal circles there can still be found division of sport according to criterion of sex, therefore we have so called "male" and "female" sports. Reasons for this division can be found in different places but it seems that gender (in) equality, which was particularly pronounced in the past, is the foundation of this understanding of sports. Tradition observes sport as "male" activity in which certain anthropological dimensions, such as body constitution or muscle strength determine success, dominantly, where participation of women in such activities becomes pointless, unnecessary and irrelevant, because they have "weaker body constitution". This approach and understanding of sport lead to the opinion that historically speaking, women in all aspect of social development didn't have equal rights in doing physical activities and sport, in general, although their needs for movement were and still are on the same level as men's. The goal was to use a descriptive method to analyze and define certain gender differences in the area of anthropological dimensions (morphology, cardiovascular system, functional abilities, body composition, somatic changes during life, ...) on the basis of which certain recommendations were given for physical activity and the training process of women. Playing sports and physical exercise are important for women's health, physical fitness and physical appearance and improve their quality of life. It can be concluded that physical activity has an extremely good and positive effect on the psychophysical state of the female organism and numerous health benefits compared to women who lead a sedentary lifestyle.

<p><strong>Introduction. </strong>The adoption of correct walking patterns is an indicator of the locomotor system readiness to establish optimal interaction between body force and the surface, and the way of creating pressure exerted by the feet during the walking cycle. The aim was to examine how the speed and characteristics of the terrain affect the distribution of force and plantar pressure during the gait of children with different levels of physical activity.</p> <p><strong>Methods.</strong> A prospective comparative study included 150 students aged 11&ndash;12 years and their parents from Banja Luka. According to the protocol, each group of subjects walked at average and maximum speed on flat and 5% inclined terrain. For the purposes of the research, the Physical Activity Questionnaire PAQ-C (The Physical Activity Questionnaire for Older Children), a survey questionnaire for parents, measurement of anthropometric parameters and Zebris strips (Zebris Medical GmbH, Germany) were used for gait analysis.</p> <p><strong>Results.</strong> When walking at maximum speed in inactive subjects, the maximum force on the left (F(148) = 14.878, p &lt; 0.001) and right (F(148) = 8.204, p &lt; 0.001) heel decreased, while in moderately and highly active subjects it grew moderately. In highly active subjects, the highest value of maximum pressure was registered (d = -1.41 for the left leg and d = 1.36 for the right leg). When the slope of the terrain changes in inactive subjects, the maximum force on the front part of both feet decreased (F(148) = 5.043, p = 0.008, d = 0). The influence of terrain characteristics was such that walking on a 5% incline almost as a rule had greater effects on inactive children, while moderately and highly physically active children gave an adequate response when walking on the 5% incline.</p> <p><strong>Conclusion.</strong> Urbanization and new developments in society impose the need to involve children in organized activities so that children acquire the skills and demonstrate the competence they face in their environment.</p>

Soil fertility is pivotal for agricultural productivity, and iron (Fe) is a critical micro- nutrient essential for a successful crop development. This study investigates a potential of ensemble machine-learning methods in geospatial prediction of soil Fe in Croatia. Using a dataset of 686 soil samples, three individual machine-learning methods, including the extreme gradient boosting (XGB), support vector machine (SVM), and Cubist, as well as their ensemble, were evaluated for the soil Fe predi- ction. The ensemble method outperformed the individual models, exhibiting a higher prediction accuracy expressed by the coefficient of determination (R2 = 0.578), with a lower root-mean-square error (RMSE = 0.837) and the mean absolute error (MAE = 0.550). The soil clay content emerged as the most influential predictor, followed by the sand content, pH values, and select bioclimatic variables. This study’s results demonstrate the effectiveness of ensemble machine learning in an accurate predicti- on of soil Fe content and contribute to an informed decision-making in sustainable agricultural land-use planning and management. By including the complementary machine-learning methods into an ensemble with the representative environmental covariates, a geospatial prediction aids to a reliable comprehension of soil proper- ties and their spatial variability.

Resumo .

Previous research on archaeological skeletal remains in Bosnia and Herzegovina rarely included odontological analysis. The objective of this research was biological profiling through odontological analysis (dental morphological characteristics, estimation of sex and dental age at death) in medieval archaeological skeletal remains samples from Krešića greblje in Paoča (Čitluk municipality, Bosnia and Herzegovina). After the excavations of the archaeological site, odontological analysis was performed before genetic analysis. The sample comprised 22 permanent teeth from 6 individuals. Odontological analysis included odontometric estimation of sex, estimation of age at death by two morphological methods, and an analysis of dental traits and dental pathology. Genetic analysis was used for sex determination. The results of odontological analysis classified all individuals as adults, with estimated age at death 20-25 in one individual, 30-35 in three individuals, 35-45 in one individual, and one individual older than 45 years of age. Odontometric sex estimation was in high concordance with sex determination by genetic analysis, and correctly classified five individuals’ sex (four females and one male). In one individual odontometric sex was assessed as female while genetic analysis revealed male. Morphological dental traits and dental pathology were investigated as well, adding to a better understanding of the odontological characteristics of the medieval population in the area of Bosnia and Herzegovina. Sex and dental age estimation given by odontological analysis provide fast and cost-effective information, and should be performed as an auxiliary before genetic analysis.

Objective. Anticoagulant therapy is a risk factor for repeated intratumoral hemorrhage and acute enlargement of a vestibular schwannoma (VS) with neurological deficits. Therefore, we describe two cases of patients on oral anticoagulant therapy with intratumoral hemorrhage in which anticoagulant therapy prior to surgical resection was discontinued. We also discuss other similar cases from the literature since this is a rare event. Case Reports. We described the two cases of intratumoral hemorrhage in acoustic neurinoma and conducted a literature review of similar cases of patients with intratumoral hemorrhage in acoustic neurinoma who were also on oral anticoagulants. Both patients presented with CN-VII palsy prior to surgery; both also fully recovered after surgery except for hearing loss on the tumor side. Our literature review found 50 cases of VS (reported as vestibular schwannomas in the literature) with intratumoral hemorrhage. From this total, 11 patients used oral anticoagulant therapy with reported poor outcomes and high mortality; 9 of these 11 cases were reported in the past 20 years. The incidence is expected to rise due to increased use of anticoagulant therapy due to onset of atrial fibrillation, atherosclerosis, and thromboembolism from longer human lifespan. Conclusion. Anticoagulant therapy represents a risk factor for intratumoral hemorrhage and acute enlargement of VS tumor mass with neurological deficits.

Environmental pollution is a big problem for all countries of the world, especially developing countries. Pollution is very present in our country and manifests itself through air, water and soil pollution, but also the increased presence of noise, ionizing and non-ionizing radiation in the environment. Environmental protection is an area of great interest for citizens and competent authorities, especially in the perspective of European integration, and represents a major challenge for the authorities of each country. The environment is one of the most important chapters in the negotiations for accession to the European Union (EU). This chapter deals with EU and Republic of Srpska regulations, with an analysis of the situation in various areas of the environment. It is the obligation of Bosnia and Herzegovina and Republic of Srpska to implement the aforementioned regulations into the national legislation. A major problem of environmental management is the implementation and application of regulations due to various factors such as: the state of industrial development, national policy, financial situation, lack of trained personnel and laboratories, etc.

Waste is a by-product of human activities and living. With the increase in the number of inhabitants, the standard of living and urbanization, the quantities of municipal waste are increasing day by day. Every segment of waste management starting from generation, through collection, storage, transport, treatment and disposal can pose a potential hazard to human health and the environment. Waste management in Republic of Srpska is organized at the regional level. Since only waste disposal is still present in the Republic of Srpska, the regional approach implies that there is a landfill on the territory of one of the local self-government units, where all local self-government units in the region dispose of waste. In addition, waste disposal in illegal and unregulated landfills is still evident. LGUs or utility companies often face a lack of money to organize waste collection, with the result that not all households are covered by waste collection. In recent years, a lot has been invested in infrastructure, such as the filling of containers and containers and the purchase of new or newer used waste collection vehicles. Future directions of improving the waste management system in Republic of Srpska must go in the direction of sustainable waste management, ie waste management in a way to reduce the negative impact on human health and the environment, as well as avoiding leaving this problem to future generations.

Harmful effect of noise to the human health are various, begining from psihological to the irreversible damage of hearing. In order to prevent negative impact to the living and working environment, especially at the urban and industrial places where noise influence is the largest, at the specific location noise estimation must be performed before sources are build. Action of protection as well as reduction of the noise based on law and technical regulations, followed by detailed investigation are mostly applied in Banja Luka, the largest city of Republic of Srpska with huge dense of population and intensive traffic jam. Beside traffic noise, which has strongest effect to the human health, theoretical predictions are also performed for directional speakers, mostly temporary active in urban location during cultural manifestation. Theoretically obtained results from simulations are projected to the map of noise, where are further defined borders between places with enlarged noise in comparatione with referent levels, given by international standards but in agreement with national legislative. Review of data and their analysis in order to determine degree of the environment contamination in the Republic of Srpska with noise is the primary goal of this study. The measurement methods and theoretical assessment tools used for detection and prediction of these physical pollutants, which tend to grow permanently due to lifestyle, are also presented.

The problem of air pollution has been a challenge for modern humanity in recent times. The environment, including the air, is burdened by a large amount of pollutants that are released into the environment. The atmosphere contains primary and secondary pollutants, emitted as basic or specific pollutants. Air pollution is present in industrial areas and larger cities, with the fact that there are no areas without any impact of air pollution. Air pollution is also present in the Republic of Srpska, as is the case in other areas. Areas of increased pollution in Republic of Srpska, with high concentrations of pollution, can further worsen the impact on the population and lead to unwanted health effects.

The level of radio frequency radiation is followed by the growth of the new telecommunication technologies and the needs of the user. In order to prevent increasing of exposure over doses of electromagnetic radiation permitted for the general population, it requires to planing the construction of antenna systems and examine the living as well as working environment in their surroundings. City zones are potentially the most vulnerable on the exposure to high-frequency non-ionizing radiation, described below with examples of sensitive locations in Banja Luka such as the vicinity of schools, kindergartens and the hospital center. Review of data and their analysis in order to determine environment contamination level in the Republic of Srpska with non-ionizing electromagnetic radiation is the primary goal of this study. The measurement methods and theoretical assessment tools used for detection and prediction of these physical pollutants, which tend to grow permanently due to lifestyle, are also presented.