: The subject of this paper is the research of the influence of different ratios of buckwheat, in concentrated feed, on the qualitative values of laying hen eggs. The study was conducted in four groups of laying hens: one control and three experimental, which were formed with respect to different proportions of buckwheat in meals. Within the first group of laying hens, a concentrated feed mixture with 10% relative share of buckwheat was used, within the second group with 20% relative share of buckwheat and within the third group with 30% relative share of buckwheat, while in the control group standard concentrated food was used. Based on the conducted research, it can be concluded that buckwheat in the meal of laying hens has positive effects on quality, physical properties, sensory properties and frequency of carrying. It was shown that there are statistically significant differences in mean values for the following variables: protein content in egg white, protein content in egg yolk (%), fat content in egg yolk (%), where the highest value was recorded in the third group of laying hens. Also, statistically significant differences were found in terms of shell weight (g), shell thickness (mm), yolk diameter (mm) and egg white pH. The highest average frequency of egg laying was found in the first group and the lowest average frequency of egg laying was in the control group. The general conclusion is that buckwheat can be used in poultry feed, because it has a much greater positive than negative effect on the production and quality characteristics of eggs for consumption.

Transplantable mouse methylcholanthrene-induced fibrosarcoma (CMC4 tumour growing in CBA/HZgr mice), characterized by lung metastases developing shortly after local tumour cell transplantation, was used as an experimental model to investigate the problem of tumour metastases after local tumour treatment. Surgery and/or irradiation were performed on locally growing tumour of particular size. Further, heavily irradiated, viable but not dividing tumour cells, imitating the situation in treated tumour-bearing organism, were injected intraperitoneally in a parallel group of treated tumour-bearing mice. The animals were killed 35 days after tumour transplantation and the number and volume of lung metastases were determined. Depending on the treatment performed, when the tumour mass was reduced or even eliminated, the number of lung metastases and their volume were significantly lower than in control mice, but the addition of tumour mass (injection of heavily irradiated tumour cells) resulted in a significant increase in lung metastases parameters, pointing to a possible role of the host’s immune reaction against the tumour. Further, the release of a simple molecule, such as nitric oxide, from tumour mass seems to be detrimental for the survival of tumour cells and subsequently their metastases through the induction of angiogenesis and possible suppression of immune reaction. Thus, complex mechanisms could be involved when a locally growing tumour is exposed to a particular therapeutic approach. Introduction Numerous approaches have been attempted in controlling tumour growth. Some of them, such as surgery, irradiation, or chemotherapy are still main clinical approaches either alone or in particular combinations. However, the fate of distant metastases after the treatment of locally growing tumour, particularly by using surgical treatment and/or tumour irradiation, is still an open question (Baum and Badwe,1994; Demicheli, 2001; Demicheli et al., 2001; Fidler, 2002; Baum et al., 2005; Khokha and Voura, 2005). Actually, numerous clinical reports point to the problem that metastasis growth could be accelerated following local tumor treatment (Demicheli et al., 2001; Benouchan and Colombo, 2005; Khokha and Voura, 2005; Mocellin et al., 2006). It should be mentioned that the immune reactivity of a tumour-bearing organism is detrimental either for tumour growth or for host survival (Heppner and Miller, 1998; Bubenik and Šimova, 2005; Indrova et al., 2008). This reactivity depends upon tumour mass and therapeutic approaches affecting local and/or disseminated tumour growth (Jurin and Suit, 1974; Heppner and Miller, 1998; Bubenik and Šimova, 2005; De Visser et al., 2006; Indrova et al., 2008). Further, it has been well documented that removal of the primary tumour reduces angiogenesis inhibitors, and a sharp spike in angiogenesis stimulators and growth factors is known to be involved in wound healing (Baum and Badwe, 1994; Baum et al., 2005). Thus, it is not surprising that tumour angiogenesis and its proliferation occur after (surgical) removal of locally growing tumour, and this act could be a trigger for “kick-starting” the growth of earlier established micro-metastases. Thus, the complex situation in a tumour-bearing organism could be further aggravated by anti-tumour therapy. In this study, transplantable, methylcholanthrene-induced fibrosarcoma in mice, characterized by lung metastases developing shortly after local tumour cell transplantation, was investigated. The surgical approach and/or irradiation were performed on a locally growing tumour of a particular size. To increase tumour mass (viable, but not growing tumour), heavily irradiated tumour cells were injected intraperitoneally (ip) in a parallel Received: March 12, 2009. Accepted: April 20, 2009. This research work was supported by the Ministry of Science of Republic Croatia (project 1-08-198). Corresponding author: Mislav Jurin, Department of Molecular Medicine, Ruđer Bošković Institute, Bijenička c. 54, 10000 Zagreb, Croatia. Phone: (+385) 1 4680091; Fax. (+385) 1 4561010; e-mail: jurin@irb.hr Abbreviation: ip – intraperitoneally. Folia Biologica (Praha) 55, 177-182 (2009)