Background: Fetal midgut volvulus is a serious fi nding with a high risk of life-threatening fetal complications. Aim: To describe a sudden intrauterine fetal death caused by midgut volvulus at term. Methods: This is a case report of a 28-year-old G2P0 patient with no signi fi cant medical history. At 34 weeks of gestation, an ultrasound revealed a cystic formation in the right upper abdominal quadrant of the fetus. In the 38th week, abrupt fetal intestinal dilatation and the absence of bowel peristalsis in what had been a normally developing fetus prompted the decision to induce labor and perform an emergency caesarean section due to terminal bradycardia. Neonatal resuscitation was attempted but unsuccessful (Apgar score 0/0/0 at 1, 5, and 15 minutes). Conclusion: In this case, the true diagnosis of a fetal midgut volvulus and the cause of fetal death were con fi rmed by autopsy and a pathologist’s fi nding.
. Aim: To assess Red blood cell Distribution Width (RDW) and platelet indices values in patients with type 2 diabetes mellitus (T2DM) and to verify its association with kidney dysfunction (KD). Patients and Methods: A cross-sectional study included 149 T2DM subjects divided into two groups with (T2DM – KD; n=52) and without (T2DM-nKD; n=97) presence of kidney dysfunction and 30 healthy subjects. White Blood Cells (WBC) count, C-reactive protein (CRP), fibrinogen, RDW, platelet indices, urea, and creatinine, were measured in all participants. Kidney function was evaluated by the estimated glomerular filtration rate (eGFR) calculated using the simplified Modification of Diet in Renal Disease (MDRD) formula. Results: T2DM-KD subjects showed statistically significantly higher values of the parameters RDW (p<0.01), Mean Platelet Volume – MPV (p<0.01), Platelet Distribution Width-PDW (p<0.01), Platelecrit-PCT (p<0.01), and Platelet Mass Index-PMI (p<0.01) compared to T2DM-nKD subjects, and statistically significantly lower values of the WBC count in T2DM-KD subjects compared to subjects suffering from T2DM without kidney dysfunction (p<0.01). ROC curve analysis revealed that RDW (sensitivity of 80.8%, specificity of 78.3%), MPV (sensitivity of 75%, specificity of 78.4 %), and PDW (sensitivity of 80.8%, specificity of 83.5%) could be used as markers in distinguishing between T2DM subjects with and without kidney dysfunction. Conclusion: This study confirms the reliability of the RDW,MPV, and PDW as simple, low cost and useful markers in distinguishing between T2DM subjects with and without kidney dysfunction.
Ten biologically active derivatives of 2,6,7-trihydroxyxanthen-3-one, previously synthesized and characterised, were investi - gated for their in vitro antimicrobial and antiproliferative activity. Compounds were tested on three bacteria, Staphylococcus aureus , Bacillus subtilis and Escherichia coli , and two fungi strains, Candida albicans , and Saccharomyces cerevisiae . The best activity against E. coli showed non-substituted compound 1 . The most potent against fungi strains was compound 7 with ortho methoxy substituent. Compound 4 exerted the most potent antiproliferative activity in the micromolar range (0.1–10 µM) on tested tumour cell lines except on SW620. Additional Western blot analyses showed increased cyclin B1 levels in HeLa cells treated with compound 4 , which is a major mitotic catastrophe’s marker and decreased levels of Wee1 and Erk ½ kinases involved in regulation of the mitotic process. The most potent compounds after in vitro tests were subjected to molecular docking simulations to evaluate enzyme binding affinity, and provide further evidence for experimentally observed biological effects in vitro .
Introduction: Healthcare organizations are faced with numerous problems, including a chronic lack of financial resources. Aim: To determine the difference in familiarity with the standards related to the quality management system between the primary, secondary and tertiary levels of health care, to determine the difference in the implementation of the quality system; determine the connection between the implementation of the quality system and the satisfaction of service users – patient. Materials and methods: Statistical processing of all data from the survey questionnaire was performed in the SPSS 17.0 package (SPSS Inc.). The level of statistical significance was set at 0.05, and all confidence intervals are given at the 95% level. The normality of the distributions was checked by Shapiro-Wilk tests when the samples were smaller than n=30, and by the Kolmogorov-Smirnov test for larger samples. Given that in most cases they indicated statistically significant deviations from normal distributions, the median and interquartile ranges were used as measures of central tendency and dispersion. Mutual comparisons of all three levels of health activity were analyzed with the Mann-Whitney U test, with Bonferroni’s correction of the level of statistical significance. The survey questionnaire was sent to the addresses of a total of 110 BiH health institutions at the primary, secondary and tertiary levels of health care. In the period June/July 2024, a total of 86 completed survey questionnaires were collected, which represents 76,18 % of all respondents. Conclusion: Research on a sample of primary, secondary and tertiary healthcare institutions in Bosnia and Herzegovina showed that the knowledge and application of the quality management system is still insufficient and points to the need to raise awareness of the importance of the application of the quality management system. Clinical hospital centers, as expected, given the application of the highest standard of medicine and the highest level of provision of health services, showed: greater knowledge of the importance of the quality management system and representation of quality assurance requirements of the ISO standard. The research also found that healthcare institutions that use international standards of quality management systems perform systematic monitoring of patient satisfaction with
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