ABSTRACTTransformations of potato (Solanum tuberosum cv. Yayla kizi) and tobacco (Nicotiana tabacum cv. Samsun NN) were achieved using tuber discs and internodes via Agrobacterium rhizogenes 8196 wild strain on MS (11) media supplemented with 5 μM Naphthalene Acetic Acid (NAA). In this study, we examined the effects of NAA on hairy root formation of potato and tobacco. Cultures of bacteria were concentrated at O.D. 600=0.2 for inoculation and MS media supplemented with 1 μM Cefotaxime (Cx) and 5 μM NAA were used for co-cultivation. Transformed hairy roots were obtained after three weeks in a regulated plant growth cabinet at 25 °C with 16 hours light followed by 8 hours dark period. DNA was extracted from these roots and digested using EcoR I and Hind III restriction endonucleases, and transformation was confirmed using the Southern blot hybridization method with [γ32P] CTP labeled Ri plasmid DNA of A. rhizogenes 8196.

The effect of lead ions on the galena and sphalerite surface properties, ethylxanthate adsorption and kinetics of ethylxanthate adsorption in alkaline medium were studied for the possible use of recycled flotation water. The flotation tests and the zeta-potential measurements were carried out and correlated with the results of the infrared attenuated total reflection spectrophotometry (ATR–IR) and UV data. Lead adsorbed on the galena or sphalerite surface as Pb(OH) + and less as Pb 2+ reduces the collectorless galena floatability from pH 7 to 9.5, but has a strong activating effect on natural sphalerite floatability (in pH range 7.6–10.5). After collection of Pb-modified minerals with K-ethylxanthate both forms of lead ethylxanthate, monolayer Pb–EX (chemically adsorbed (EX) – ), and multilayer, physically adsorbed Pb(EX)2, were detected on the mineral surfaces. Sphalerite floated nearly 100% in the examined pH range, but galena floated better only from pH 8 to 9.5 (85–95%). The main surface reaction in pH range 8–9.5 is probably the ion-exchange reaction M–Pb–OH + (EX) – = M–Pb–EX +(OH) – (M – mineral), which contributes to the chemical adsorption of (EX) – as monolayer –Pb–EX, on the surface of either of the mineral. The reaction between Pb-treated mineral and (EX) – ion is the reaction of pseudo-first order (on Pb-modified galena – rate constant is k = 0.711 min –1 ; on Pb-modified sphalerite k = 0.102 min –1 ).

Dometi i ogranicenja konvencionalne kompjuterizirane tomografije u dijagnostici disekcije aorte