________________________________________________________________________________________ KRKALIC, L., E. SATROVIC, T. GOLETIC, P. DŽAJA, K. SEVERIN: Chlamydophila abortus infection in a fl ock of goats in Bosnia and Herzegovinaa case report. Vet. arhiv 85, 359-368, 2015. ABSTRACT The aim of this research was to determine the presence of Chlamydophila abortus (C. abortus) infection in one fl ock of goats with a previously recorded history of reproductive failures (abortion, stillbirths, weak born kids) and long-term poor reproductive performances. The affected fl ock was from the southern region of Bosnia and Herzegovina (B&H) and consisted of 48 goats kept in semi-intensive conditions. Blood samples and vaginal swabs were collected twice during 2012 and 2013 and the sample size was estimated at a 95 % confi dence level, with predicted prevalence of 20 %, using the recommendations for determining the required sample size necessary to detect the presence of disease in a fl ock. A representative sample from this fl ock was taken by simple random sampling. In the total of 12 blood sera that were tested for the specifi c antibodies against C. abortus, with the use of enzyme-linked immunosorbent assay (CHEKIT® Chlamydophila abortus Antibody Test Kit), the results showed that 11 (91.7 %) sera were positive for C. abortus antibodies. Vaginal swabs from all animals were analysed by a modifi ed Chlamydiaceae-specifi c rtPCR test, targeting the 23S rRNA gene, to determine the presence of known Chlamydiaceae, and three (25 %) samples were positive. These positive samples were subsequently tested with a test targeting the ompA gene region (ompA-rtPCR) specifi c for Chlamydophila abortus. All three samples were also positive using this test.

Introducao: Alguns inseticidas utilizados em saude publica inibem as colinesterases, levando ao acumulo de acetilcolina nas terminacoes nervosas, provocando alteracoes no sistema nervoso central. Objetivos: Avaliar o padrao de normalidade dos niveis de colinesterase de agentes de endemias de duas Microrregionais de Saude do Ceara. Metodos: Levantamento de dados em duas unidades do Laboratorio Central de Saude Publica (LACEN), referentes a dosagens de colinesterase de 81 agentes de endemias das 20a e 21a Microrregionais de Saude, medidas entre os anos de 2014 e 2015. Os agentes eram procedentes de seis municipios, nomeados em A e B (21a Microrregional); C, D, E e F (20a Microrregional). Resultados: Os agentes de A encontravam-se com um nivel de colinesterase medio de 7.460,8 U/L (±1.232,2 U/L), e os de B, 6.941,6 (±1.122,3 U/L). Os niveis colinergicos dos agentes da 20a Microrregional variavam de 3.406 U/L a 12.108 U/L, com media de 8.318 U/L (±2.334 U/L). Nove homens apresentavam alteracao nos niveis colinergicos, sendo cinco com valores abaixo do limite inferior de normalidade (4.620 U/L), quatro, acima do limite superior (11.500 U/L) e ¼ deles apresentava um nivel de 4.470 U/L, uma diferenca de apenas 150 unidades para a taxa minima de normalidade. Conclusoes: Constataram-se alteracoes nos niveis de colinesterase de 11,1% dos agentes, e aproximacao do valor limitrofe inferior de normalidade em 25% dos casos, indicando a necessidade de monitoramento desses niveis e conhecimento do estado de saude desses trabalhadores.