The existing building stock energy consumption accounts for about 38% of final energy consumption in Republic of Serbia. 70% of that energy is consumed by residential sector, mostly for space heating. This research is addressed to the single family house building placed in the Belgrade city. The house has ground and first floor with total heating area of 130 m2 and pellet as space heating source. The aim of this paper is to evaluate energy and economic analysis for different heating systems. Several homeheating were compared: Option 1 (biomass combustion boiler using pellet as a fuel), Option 2 (gas combustion boiler) and Option 3 (heat pump). The building performance was evaluated by TRNSYS 17 simulation code. Results show estimated savings using renewable energy sources. [Projekat Ministarstva nauke Republike Srbije, br. III42008]

: Nutrition is recognised as one of the major factors that can influence the development of cancer. Heterocyclic amines formed during the cooking of meat are genotoxic carcinogens and consumption of meat positively correlates with certain types of human cancer. On the other hand, it has been reported that many plant components reduce the genotoxic activities of carcinogens, including HCAs. In this study we investigated the antigenotoxic potential of the monoterpenes linalool, myrcene and eucalyptol against two food-borne carcinogens: 2-amino-1-methyl-6-phenylimidazo[4-5- b ]pyridine (PhIP) and 2-amino-3-methylimidazo[4,5-f]-quinoline (IQ). The study was performed by the comet assay method in metabolically active HepG2 human hepatoma cells. In addition, the lipid peroxidation TBA assay was used to determine the anti-oxidative potential of these monoterpenes. To evaluate the antigenotoxic potential, cells were treated simultaneously with 0.01, 0.1 or 1 μg/mL of monoterpenes and either 90 μmol/LPhIP or 0.75 mmol/L IQ. The DNA damage induced by PhIP was reduced significantly (by 40-63%) by each of the three monoterpenes, while inhibition of IQ-induced DNA damage was less efficient (12-26% reduction) and limited to myrcene and eucalyptol. Lipid peroxidation was significantly inhibited by linalool and myrcene, with IC 50 values of approximately 30 μg/mL and 300 μg/mL, respectively. Eucalyptol showed only weak antioxidant activity (15% inhibition at 500 μg/mL). Taking into account the fact that reactive oxygen species (ROS) are formed during metabolic processing of PhIP, we assume that more efficient protection against PhIP-induced DNA damage was probably due to antioxidative activity of the monoterpenes or their metabolites, but the ability to interfere with metabolic activation of PhIP and IQ could also be involved.

This paper presents R&D project of multi fuel concept (MFC) for future coal-based power plants, demonstrated on example of cofiring Middle-Bosnia brown coal with waste woody biomass and natural gas. Pulverised Combustion (PC) lab-scale furnace has been used for the cofiring tests, varying up to 20%w portion of biomass and up to 10%th portion of natural gas in the fuel mix. Tests were purposed to optimize the combustion temperature, air distribution, including Over Fire Air System (OFAS), fuel combination and fuel distribution, including reburning concept, as function of emissions and combustion efficiency estimated through the ash deposits behaviours and unburnt. Considering application of proposed MFC in case of TPP Kakanj unit 6 (118 MWe) set here as a referent power plant, temperature levels and fuel distributions for lowest emissions of CO2 and NOx were found during lab tests, provided that combustion efficiency is at an acceptable level. Derived research results yield input data for calculation sustainability indicators of MFC for the referent power plant, considering 6 fuel options - different combinations of coal, biomass and natural gas. Single criteria analysis and multicriteria sustainability assessment have been done, giving an advantage to the options of cofiring coal with woody biomass and natural gas in the case demonstrated.

Introduction. Synchronous multicentric cerebral gliomas are uncommon brain tumors, mostly malignant, with unknown pathogenesis, unfavorable prognosis and still controversial management. Preoperative differentiation from other multiple brain pathologies by conventional magnetic resonance imaging (MRI) is often difficult, but supplemental use of advanced magnetic resonance techniques should allow the tumor biology to be predicted, and an appropriate treatment strategy planned. Case report. We report the case of a 59-year-old man with double synchronous multicentric cerebral lesions, which had initial MRI and diffusion-weighted imaging presentation as left parietal metastasis and ipsilateral amygdalo-hippocampal low-grade glioma. However, magnetic resonance spectroscopy (MRS) of both lesions showed different metabolite profiles of malignant glioma. En bloc resection of the easily accessible parietal lesion revealed glioblastoma with methylated O6-methylguanine-DNA methyltransferase (MGMT) gene promoter. Subsequently, the patient was treated with temozolomide (TMZ)-based chemoradiation according to Stupp’s protocol, with continuous standard (5/28) adjuvant TMZ in 12 courses. Despite prolonged stabilization of the disease with good life-quality during treatment, the patient died 19 months after diagnosis. The time to tumor progression estimated by MRI was 17 months. Conclusion. MRS significantly improved the differential diagnostic accuracy of conventional MRI in our patient. In accordance with reviewed literature data, the younger age, good initial performance status and methylated MGMT gene promoter were all favorable predictors of longer survival in the reported case. Resection of at least one easily accessible tumor lesion, followed by TMZ-based chemoradiation, with continuous adjuvant TMZ in more than 6 standard courses, seems currently to be the most beneficial therapeutic option for such cases.

Demencija je progresivna bolest koja dovodi do gubitka mentalnih sposobnosti, što rezultira promjenom memorije, te gubitka socijalnih sposobnosti. Demencija nastaje zbog stanja koja izazivaju promjenu mišljenja, pamćenje, zaključivanje i govor. Demencija je postala sve učestalija, a može se otkriti već u srednjim godinama iako je u starosti mnogo češća. Pozitivna veza između ishrane i nastanka mentalnih poremećaja ogleda se i u jakoj korelaciji između unosa rafinisanog šećera i smanjenja stava svijesti oboljelih od šizofrenije i nastanka depresije. Mentalne promjene u toku starenja i postepeno odvajanje od aktivnog društvenog života, obično se emotivno odražavaju u prvoj fazi starenja bjekstvom u hranu, a u drugoj fazi poslije 75. godine apatijom, kada i hrana gubi svoju privlačnost. Demencije predstavljaju jedan od najznačajnijih savremenih javnozdravstvenih problema. Dugotrajan nutritivni deficit u ishrani povezuje se sa depresivnim raspoloženjem, anksioznošću i kongnitivnim propadanjem.

This paper presents the first estimation of polymorphism of the Bosnian-Herzegovinian-Croatian Shepherd dog Tornjak in Bosnia and Herzegovina using 10 microsatellite loci, which are an integral part of StockMarks® for Canine Genotyping Kit (Applied Biosystems, Foster City, CA, USA). Ten microsatellite loci used in this study are appropriate for assessing the genetic diversity for this breed. Measures of genetic diversity were estimated based on allelic and genotypic calculations, observed (HO) and expected (HE) heterozygosities, deviations from Hardy-Weinberg equilibrium and polymorphism information content (PIC). The lowest genetic diversity was estimated for locus PEZ20, and the highest for PEZ6 locus. Observed and expected mean heterozygosities were 0.7261 and 0.7392, respectively. Statistically significant deviation (p<0.05) from Hardy-Weinberg equilibrium was found for PEZ1, PEZ12, PEZ3 and PEZ6 loci. The PIC values suggested that all markers (100%) are very informative (PIC > 0.5) in terms of their suitability for genetic diversity studies. When all observed parameters are taken into account (observed and expected heterozygosities, PIC, number of detective and effective alleles, number of detected and maximum possible genotypes, major allele frequency and major allele frequency index), we can conclude that PEZ6 locus shows the highest genetic diversity while PEZ3 displays the lowest. However, assuming values of observed and expected heterozygosities, as well as PIC, we consider loci PEZ20 to be the least diverse, but this locus has more effective alleles and more genotypes present than PEZ3. These preliminary results are the first genetic diversity survey of the Bosnian-Herzegovinian-Croatian Shepherd dog Tornjak in Bosnia and Herzegovina and could be useful to the dog breeders in designing and managing breeding strategies. Summarizing the information above, we can conclude that the population of the Bosnian-Herzegovinian-Croatian Shepherd dog Tornjak from B&H is not affected by substantial loss of genetic diversity. Results of our study indicate presence of reasonably high level of genetic variability and lead to a better understanding of this dog breed.

Steroid hormones such as estrogen play a central role in postnatal female physiology and their effects are exerted through its receptors. The estrogen receptor gene is one of the first and most extensively investigated candidate genes for reproductive traits in pigs, especially for litter size. The aim of the study was to investigate the estrogen receptor gene polymorphism using endonuclease PvuII and its association with the number of stillborn (NSB) and number of mummified (NMUM) piglets in the first, second, third and subsequent parities and in overall parities. Topigs 20 sows (n=101) from 1st to 7th parities were analyzed. Estrogen receptor genotypes were detected by the polymerase chain reaction-restriction fragment length polymorphism method. Two alleles (A and B) were identified with three genotypes. Alleles (A and B) and genotype frequencies were determined. Comparison of observed and expected genotype frequencies was performed using the χ2-test and considerable deviation from Hardy-Weinberg principle was found. All data were analyzed using the General Linear Model. Statistical analysis showed a significant difference (P<0.05) in NMUM between the AB and BB genotypes in the third and subsequent parities. The statistical significance of differences between AA and BB genotypes tended to be lower (P<0.1) in the third and subsequent parities in NMUM. The sows with A allele had less NSB indicating a beneficial effect of this allele, unlike B allele in NMUM. The results obtained will contribute to the understanding of the effect of ESR genotype on NSB and NMUM and substantiate genetic evaluation of litter size traits in pigs.