One of the most important health problems of today's society is hypokinesia accompanied by obesity. The assumption is that reduced physical activity has a large negative impact on the school population. The problem of reduced commitment and involvement of pupils in extracurricular activities is increasingly evident in recent times, which results in a number of negative health effects on the human body of an individual. This study comprised a group of older school children from first to fourth year of high school in Pale. The total sample consisted of 127 female pupils aged 15 to 18 years. As a way of gathering the required information we used an anonymous questionnaire of open type in order to collect information about student involvement in extracurricular sports activities. The survey was conducted in November 2012 and all the pupils voluntarily participated in the study. Based on the survey results have been obtained the necessary information to reflect the relative lack of physical activity, of the studied population. Of the total sample of female pupils, the survey confirmed that only about 22% are physically active pupils. The alarming are the results which showed that with the increasing age of pupils they are less physically active, and in the higher years is evident negative trend in physical activity (sports).

Objective: The purpose of our retrospective study was to investigate the necessity of some laboratory testing in patients with acute bronchiolitis before and after treatment. Methods: We have taken blood samples of all children puncturing the cubital vein, and analyzed it using the Colter appliances-automatic counter blood count, for analyzes of some erythrocytes, leukocytes, platelets, differential blood count, Hct, Hb. CRP concentration in serum of patients determined by laser nephelometry with CardioPhase® high sensitivity C-reactive protein (hsCRP). For assessment of acid-base status and arterial blood gas analysis were used ABL5 and ABL700 Radiometer Copenhagen. We monitored the following parameters: pH, pCO2, HCO3−, total CO2, base excess, pO2, SpO2. Results: There was a significant improvement of hypoxemia after management of acute bronchiolitis in the form of a significant increase in average values of pO2 and SpO2 after treatment of acute bronchiolitis. The mean value of the number of leukocytes and value of CRP in children were significantly decreased before and after management of acute bronchiolitis. There was no significant difference in duration of hospitalization in term and preterm newborns. Conclusion: No diagnostic tests are used routinely. However, there is an improvement of hypoxemia after management of acute bronchiolitis in children.

The aim of this study was to determine the distribution of κ-casein (κ-CN) and β-lactoglobulin (β-Lg) genotypes in the autochtonous (Busha) and dairy (Holstein-Friesian, HF) cattle breeds with PCR-RFLP (polymerase chain reaction restriction fragment length polymorphism). For the amplification of κ-CN and β-Lg gene fragments specific primers were used. After digestion with specific endonucleases genotypes were determined for both genes in 18 Busha and 19 HF cows. The results showed that κ-CN gene was represented with the AA genotype in 31.58 % HF cows, AB in 52.63 % cows, whilst the genotype BB was found in 15.79 % cows only. Among the examined Busha cattle 44.44 % cows had AA genotype and 55.56 % genotype AB for κ-CN. As for β-Lg gene in HF breed, AA genotype was found in 26.31 % cows, AB in 63.16 % and BB in 10.53 % cows. In Busha cows the following genotypes were established for β-Lg gene: AA in 44.44 % cows and AB in 55.56 %, whilst BB genotype was not found. These results indicate that Busha cows had a higher presence of A allelic forms of both genes (k-CN and β-lactoglobulin) than HF cows.