By observing the equivalence of assertions on determining the jump of a function by its differentiated or integrated Fourier series, we generalize a previous result of Kvernadze, Hagstrom and Shapiro to the whole class of functions of harmonic bounded variation and without finiteness assumption on the number of discontinuities. Two results on determination of jump discontinuities by means of the tails of integrated Fourier-Chebyshev series are derived.

Stabilin-1 is a multifunctional scavenger receptor expressed on alternatively-activated macrophages. Stabilin-1 mediates phagocytosis of “unwanted-self” components, intracellular sorting, and endocytic clearance of extracellular ligands including SPARC that modulates breast cancer growth. The expression of stabilin-1 was found on tumor-associated macrophages (TAM) in mouse and human cancers including melanoma, lymphoma, glioblastoma, and pancreatic insulinoma. Despite its tumor-promoting role in mouse models of melanoma and lymphoma the expression and functional role of stabilin-1 in breast cancer was unknown. Here, we demonstrate that stabilin-1 is expressed on TAM in human breast cancer, and its expression is most pronounced on stage I disease. Using stabilin-1 knockout (ko) mice we show that stabilin-1 facilitates growth of mouse TS/A mammary adenocarcinoma. Endocytosis assay on stabilin-1 ko TAM demonstrated impaired clearance of stabilin-1 ligands including SPARC that was capable of inducing cell death in TS/A cells. Affymetrix microarray analysis on purified TAM and reporter assays in stabilin-1 expressing cell lines demonstrated no influence of stabilin-1 expression on intracellular signalling. Our results suggest stabilin-1 mediated silent clearance of extracellular tumor growth-inhibiting factors (e.g. SPARC) as a mechanism of stabilin-1 induced tumor growth. Silent clearance function of stabilin-1 makes it an attractive candidate for delivery of immunomodulatory anti-cancer therapeutic drugs to TAM.

U radu je opisana problematika vezana uz generiranje i zbrinjavanje mulja s UPOV-a (uređaja za prociscavanje otpadnih voda) u Hrvatskoj. Poseban osvrt je dan na mogucnost i opravdanost koristenja pepela dobivenog spaljivanjem mulja. Naglasak je stavljen na ugradnju pepela u betonskoj industriji, pri proizvodnji cementnog morta i betona. U radu su opisani rezultati do sada provedenih istraživanja u okviru svjetske prakse, s primarnim osvrtom na utjecaj ugradnje pepela na karakteristike cementnog morta i betona.

Pathogens are disease-producing microorganisms. Bacteria, mycoplasma, rickettsia, fungi and viruses are among the naturally occurring pathogens. Toxins are poisons produced through the metabolic activities of living organisms. They are organic compounds, such as proteins, polypeptides and alkaloids, that come from a variety of biological sources. Pathogens and toxins are normal constituents of the environment. However, industrial processes for fermentation and synthesis are capable of producing large quantities of pathogens and toxins for release into the environment by military or terrorist actions. The human body is vulnerable to injury from harmful exposures to environmental pathogens and toxins. Identification and quantification of these pathogens and toxins are important for protecting the body from such injuries by promptly initiating countermeasures or therapeutic regimens. Some of the environmental pathogens and toxins have been described and discussed in other chapters of this book. In this chapter, the chemical, physiochemical, biochemical and immunochemical principles serving as the basis for the detection of pathogens and toxins are reviewed and some of the technological applications of these principles to their detection are described.

The yield potential of ten plum cultivars (‘Cacak's Beauty’, ‘Cacak's Best’, ‘Cacak's Fruitful’, ‘California Blue’, ‘Elena’, ‘Hanita’, ‘Katinka’, ‘Renclode Althan’, ‘Stanley’, and ‘Top’), exhibited through the structure of buds on long bearing shoots, was analyzed under the agro-ecological growing conditions of the Banja Luka region for two fruiting seasons (2012 and 2013). Long bearing shoots with collateral buds were the most productive type of fruiting branches, which is why the structure of buds on these branches is the basis for defining specific cultural measures in order to boost yield potential. Based on the established structure of buds on the nodes along the long bearing shoots and the established yield potential, the plum cultivars evaluated were classified into 4 groups: 1) cultivars with a predisposition to have a high yield potential, without need for specific treatments in fruiting control (‘Cacak's Beauty’ and ‘Top’); 2) cultivars with a predisposition to have a high yield potential with the application of cultivar-specific cultural measures for control of fruiting (‘Cacak's Best’, ‘Elena’, and ‘Stanley’); 3) cultivars that require differentiated cultural measures in the control of yield potential for regular fruiting (‘Cacak's Fruitful’, ‘California Blue’, and ‘Katinka’) and 4) cultivars that require innovative growing systems and specific cultural treatments to control fruiting (‘Hanita’ and ‘Renclode Althan’).

Detection of viruses presence were carried out for the 225 a of pome and stone fruit trees from the collection of the Genetic Resources Institute of University of Banja Luka, located within the Botanical Garden of the University, tested by DAS-ELISA. The pome fruit trees were analyzed on presence of the following viruses: Apple Chlorotic Leaf Spot Virus (ACLSV), Apple Stem Grooving Virus (ASGV), Apple Stem Pitting Virus (ASPV) and Apple Mosaic Virus (ApMV). The stone fruits were analyzed on presence of Plum Pox Virus (PPV), Prune Dwarf Virus (PDV) and Prunus Necrotic Ring Spot Virus (PNRSV). All samples were tested serologically by DAS-ELISA. In addition to this, virus negative pear and apple accessions were tested for 'Candidatus Phytoplasma mali' and 'Candidatus Phytoplasma pyri' presence using nested-PCR/RFLP analyses.

Doxorubicin is a very potent chemotherapeutic drug, however its side effects limit its clinical use. The aim of this research was to investigate the properties of a fullerenol/doxorubicin nanocomposite, its potentially cytotoxic and genotoxic effects on malignant cell lines, as well as its toxicity towards zebra fish embryos. Chromatographic, NMR and mass spectral analysis of the nanocomposite imply that interactions between doxorubicin and fullerenol are non-covalent bonds. The stability of the nanocomposite was confirmed by the use of atomic force microscopy, dynamic light scattering and transmission electron microscopy. The nanocomposite, compared to the free doxorubicin at equivalent concentrations, significantly decreased the viability of MCF-7 and MDA-MB-231 cells. The flow cytometry results indicated that doxorubicin-loaded fullerenol could remarkably increase the uptake of doxorubicin suggesting that fullerenol might be a promising intracellular targeting carrier for the efficient delivery of antitumor drugs into tumor cells. The nanocomposite also affected cell cycle distribution. A genotoxicity test showed that the nanocomposite at all examined concentrations on MCF-7 and at lower concentrations on MDA-MB-231 cells caused DNA damage. Consequently, cell proliferation was notably reduced when compared with controls. Results of the zebrafish embryotoxicity assay showed a decreased overall toxicity, particularly cardiotoxicity and increased safety of the nanocomposite in comparison to doxorubicin alone, as manifested by a higher survival of embryos and less pericardial edema.

Kadifica ( Tagetes patula L.) se ubraja u grupu jednogodisnjih cvjetnih kultura, a zbog svojih dekorativnih osobina posljednjih godina zauzima sve veci udio u proizvodnom asortimanu proizvođaca cvijeca u Bosni i Hercegovini.Sve veca potražnja za rasadom kadifice namece potrebu intenziviranja njene proizvodnje i podizanje njene kvalitete, a jedan od nacina kojim se to pokusava postici je primjena razlicitih stimulatora rasta. Cilj ovog rada je bio ispitati uticaj stimulatora rasta Bio-algeena S-92, Slavola i Ergonfilla na parametre rasta i razvoja kadifice ( Tagetes patula L.). Od parametara rasta i razvoja kadifice u ovom istraživanju ispitivani su: sadržaj pigmenta u listovima i povrsina listova, te precnik cvijeta i broj cvjetnih glavica po biljci. Rezultati istraživanja su pokazali da ni jedan od primijenjenih stimulatora rasta nije iskazao statisticki znacajan uticaj na povecanje estetski važnih parametara kvaliteta cvijeca: precnika cvijeta i broja cvjetnih glavica na biljci iz cega se može zakljuciti da kadifica nije zahtjevna kultura za uzgoj jer je u stanju uspjesno razviti svoje cvjetove cak i ako joj se u toku uzgoja ne dodaju stimulatori rasta. Preduslov za ostvarenje navedenog je da supstrat u kojem se kadifice uzgajaju svojim fizickim i hemijskim karakteristikama zadovoljava sve kriterije potrebne biljkama za njihov uspjesan rast i razvoj.

Cilj ovog istraživanja je bio ispitati uticaj primjene razlicitih koncentracija sintetski proizvedenih biljnih hormonana parametre razvoja presadnica paprike ( Capsicum annuum L. cv. 'Sivrija'). Biljni hormoni koristeni u ovom ogledu bili su indol-sircetna kiselina (IAA), indol-butilna kiselina (IBA) i benzil adenin (BA), a svaki od njih primijenjen je u pet razlicitih koncentracija: 100, 300, 500, 1000 i 3000 mg l -1 .Od parametara kvaliteta presadnica paprike u ovom istraživanju ispitivani su: masa svježe tvari korijena, broj korijenovih dlacica po jedinici povrsine korijena, povrsina listova, te sadržaj fotosintetskih pigmenata u listovima.Najveci uticaj na povecanje ispitivanih parametara kvaliteta presadnica paprike je iskazala primjenahormonaIAA i IBA u koncentracijama 100 i 300 mg l -1 , te se iste preporucuju za primjenu kod uzgoja presadnica paprike ( Capsicum annuum L. cv. 'Sivrija').U varijantama gdje su hormoni IAA, IBA i BA primijenjeniu koncentracijama od 500 i vise mg l -1 mlade presadnice paprike nisu uspjele nastaviti svoj životni ciklus, te je doslo do njihovog venuca, sto se može objasniti cinjenicom da previsoka koncentracija hormona može iskazati i herbicidni ucinak na biljku, posebno u ranim stadijima razvoja biljke.

Since agriculture is the highest consumer of water, there are many trials to improve water use efficiency especially in arid countries. Thus, in the world are developed many irrigation techniques that could decrease water use. In this paper is analyzed new irrigation technique (AutoAgronom) used in greenhouse cucumber (two varieties Rocky and Champion) production in order to improve water use in Israel. AutoAgronom is tested against conventional system where drip irrigation was used. AutoAgronom (AA) system follows pH, Electrical conductivity, oxygen level and nitrates on which basis irrigation was done. Obtained results strongly recommend use of AA system in vegetable production. In variety Champion irrigated by AA system (9.167 kg/m 2 ) obtained yield was 20% higher in comparison with the same variety under conventional drip irrigation. Quantity of irrigation water, as well as fertilizers using AutoAgronom system are saved up to 9.7%, while phosphoric acid is saved up to 8.3%.