Background:Global DNA methylation has been reported to be associated with urothelial cell carcinoma (UCC) by studies using blood samples collected at diagnosis. Using the Illumina HumanMethylation450 assay, we derived genome-wide measures of blood DNA methylation and assessed them for their prospective association with UCC risk.Methods:We used 439 case–control pairs from the Melbourne Collaborative Cohort Study matched on age, sex, country of birth, DNA sample type, and collection period. Conditional logistic regression was used to compute odds ratios (OR) of UCC risk per s.d. of each genome-wide measure of DNA methylation and 95% confidence intervals (CIs), adjusted for potential confounders. We also investigated associations by disease subtype, sex, smoking, and time since blood collection.Results:The risk of superficial UCC was decreased for individuals with higher levels of our genome-wide DNA methylation measure (OR=0.71, 95% CI: 0.54–0.94; P=0.02). This association was particularly strong for current smokers at sample collection (OR=0.47, 95% CI: 0.27–0.83). Intermediate levels of our genome-wide measure were associated with decreased risk of invasive UCC. Some variation was observed between UCC subtypes and the location and regulatory function of the CpGs included in the genome-wide measures of methylation.Conclusions:Higher levels of our genome-wide DNA methylation measure were associated with decreased risk of superficial UCC and intermediate levels were associated with reduced risk of invasive disease. These findings require replication by other prospective studies.

The aim of this study was to clarify the clinical role of CD44 expression in ovarian serous cancer, and its relation to clinicopathologic prognostic factors, disease free survival and overall survival (OS). Immunohistochemical staining for CD44 was performed on 81 formalin-fixed, paraffin-embedded tumor sections. CD44 expression was found in 43% of ovarian carcinoma samples. Correlations between categorical variables were studied using the &khgr;2 and the Mann-Whitney U test. For survival analysis, the Kaplan-Meier method, the log-rank test and the Cox proportional hazard regression model were used. We did not find any statistically significant difference in the distribution of respondents according to clinical stage of the disease, tumor grade or the presence of vascular invasion in relation to the expression of CD44. According to the results of uninominal analysis, early International Federation of Gynecology and Obstetrics (FIGO) stage of the disease (P=0.003) was associated with longer disease free survival, while the expression of CD44 (P<0.001), FIGO stage III and IV (P=0.009) and the finding of vascular invasion (P=0.005) was related to a shorter OS. In conclusion, we proved that positive CD44 immunoexpression is a independent prognostic indicator of shorter OS of patients with ovarian serous cancer.

BACKGROUND AND AIM Drugs used chronically by patients with diseases of the cardiovascular system (group C of the ATC classification) may act on adrenergic receptors and/or certain ion channels, which gives them the potential to interact with the action of local dental anesthetics. The aim of the study was to investigate the effect of systemically administered chronic cardiovascular medication (oral route) on the efficacy of intraoral local anesthesia in patients with diseases of the cardiovascular system. PATIENTS AND METHODS This was a prospective cohort study which analyzed the efficacy of local terminal anesthesia (onset of anesthesia, duration anesthetized area) in the upper jaw of 70 patients: 40 patients on medication for cardiovascular system disorders and 30 patients who were not using these drugs (the control group). The following cardiovascular drugs were used: beta blockers, angiotensin converting enzyme inhibitors, calcium channel blockers, vasodilatators, diuretics, angiotensin receptor blockers, antiarrhythmics, statins and alfa blockers. RESULTS The onset of anesthesia on the vestibular side was faster in those taking cardiovascular drugs (40.50±19.87 s) than the control patients (58.93±31.07 s; P = 0.004) and duration of anesthesia on this side was shorter. Although the difference was not significant, it was evident that on vestibular and palatal side the anesthetized area was more rapidly reduced in the patients taking cardiovascular drugs. The duration of cardiovascular therapy also had a significant impact on the anesthetized area. CONCLUSION Drugs acting on cardiovascular system may influence the effect of local anesthetics used in dentistry, possibly through interaction with autonomic receptors and ion channels.

Mammary analogue secretory carcinoma (MASC) is a recently described low-grade malignant tumor of the salivary glands, biologically and morphologically equivalent to secretory breast carcinoma. We give a brief overview of this new entity, including morphological, immunohistochemical, molecular-genetic, clinical, epidemiologic features, differential diagnosis, and outcome results.

Although it is widely accepted that robots will be used in everyday contexts in near future, many people feel anxious and hold negative attitudes toward robots. This negative reaction might be stronger when users come into direct physical contact with them, particularly when touch is required between robots and humans, (e.g., when using robots as assistants to help elderly people at home). Intergroup contact research in social psychology has proposed various forms of contact as a means to reduce negative feelings toward outgroup members. The present study examined how Contact Type (Actual vs. Imagined) and Contact Modality (Look vs. Touch) with a NAO robot would impact attitudes toward NAO compared to a no-contact control condition. Results showed that nearly any type of contact effectively reduced negative emotions compared to the control condition. However, for participants with preexisting negative emotions toward robots, contact sometimes produced more negative attitudes. We discuss these findings and the resulting implications for future research.

Assistive robots designed to support independently living older adults are expected to solve diverse physical, social and mental challenges related to aging. This paper presents how older adults-the potential users of assistive robots-interpret robotic technologies in terms of their usefulness and fit in their everyday lives. We interviewed 7 older adults aged 57-72, showing them photos and videos of assistive robots designed with their age group in mind. Most participants saw the robots as potentially useful in general terms, yet also commented that “those robots are not for me.” An analysis of their discussion about the robots identified several aspects of robot design that participants found problematic, particularly negative representations of aging depicted in robot use scenarios. Participants saw issues related to aging as important daily problems, but did not appreciate the manner in which robotics was expected to address those issues. They were particularly sensitive to the dominance of stereotypes defining aging primarily in terms of disability in robotics discourse and design. Based on participant feedback, we suggest a broader understanding of the experience of aging as the basis for future assistive robot design for this population, and conclude by discussing related robot design possibilities.

This paper investigates whether empathy towards an interactive trash bin, particularly empathy induced by the bin's anthropomorphic appearance and behavior, affects people's evaluations of the artifact and their intention to use it to recycle. We performed an online survey in which 344 participants viewed a video showing a person interacting with a recycling bin in one of three conditions: non-interactive/control, non-anthropomorphic interactive, and anthropomorphic interactive. We measured people's perceptions of the bin's anthropomorphism and animacy, their empathy and anxiety towards and enjoyment of the bin, their perceptions of its sociability and usefulness, and finally their intentions to use the bin. The results suggest that the level of anthropomorphism attributed to the artifact by participants positively affected their empathy towards the recycling bin, which in turn directly influenced their intentions to use the bin.

Aim To examine the presence of morphologic variations of occipital sulci patternsin patients with schizophrenia and migraine headacheregarding gender and laterality using magnetic resonance imaging (MRI). Methods This study included 80 patients and brain scans were performed to analyze interhemispheric symmetry and the sulcal patterns of the occipital region of both hemispheres. Average total volumes of both hemispheres of the healthy population were used for comparison. Results There was statistically significant difference between subjects considering gender (p=0.012)with no difference regarding age(p=0.1821). Parameters of parieto-occipital fissure (p=0.0314), body of the calcarine sulcus (p=0.0213), inferior sagittal sulcus (p=0.0443), and lateral occipital sulcus (p=0.0411) showed statistically significant difference only of left hemisphere in male patients with schizophrenia with shallowerdepth of the sulcus. Conclusion Representation of neuroanatomical structures suggests the existence of structural neuroanatomic disorders with focal brain changes. Comparative analysis of occipital lobe and their morphologic structures (cortical dysmorphology) in patients with schizophreniausing MRI, according to genderindicates a significant cortical reduction in the left hemisphere only in the group of male patients compared to female patients and the control group.