Introduction: Treatment of cancer has been subject of great interest. Researchers are continuously searching for new medicines. In this sense, ruthenium complexes have big potential. Some evidences suggest that ruthenium compounds possess anticancer activities. We synthesized two recently published ruthenium(III) complexes with bidentate O,N and tridentate O,O,N Schiff bases derived from 5-substituted salicylaldehyde and aminophenol or anilineare. These compounds showed affinity for binding to the DNA molecule, however, insufficient data are available regarding their possible toxic effects on biological systems.Methods: In the present study we evaluated genotoxic, cytotoxic, and cytostatic effects of Na[RuCl2(L1)2] and Na[Ru(L2)2], using the Allium cepa assay.Results: Different toxic effects were observed depending on the substance, tested concentration, and endpoint measured. In general, the tested compounds significantly lowered the root growth and mitotic index values as compared to the control group. Additionally, a wide range of abnormal mitotic stages, both clastogenic and non-clastogenic were observed in the treated cells. Na[RuCl2(L1)2] significantly increased the frequency of sticky metaphases, chromosome bridges, micronuclei, impaired chromosome segregation, as well as number of apoptotic and necrotic cells over the controls. In contrast, Na[Ru(L2)2] did not show significant evidence of genotoxicity with regard to chromosome aberrations and micronuclei, however, significant differences were detected in the number of apoptotic and necrotic cells when the highest concentration was applied.Conclusions: In this study we demonstrated antiproliferative effects of Na[RuCl2(L1)2] and Na[Ru(L2)2]. At clinical level, these results could be interesting for further studies on anticancer potential of the ruthenium(III) complexes using animal models.

UDK 631.41:631.482(1-751.2 Una)(497.6) The paper presented results of a comparison of alluvial soils of different land use at two sites within the National Park "Una". The sites were related to a natural meadow (BUK) and artificial meadow (KLISA). The main objective of the research, in addition to the physical and chemical parameters of soil quality, was to determine the distribution of the total content of cadmium (Cd), nickel (Ni) and arsenic (As). The total content of these elements were observed in composite samples at two depths of 0-10 cm and 0-20 cm and horizons profile. Their total content was measured by atomic AAS. The results were analyzed using Kruskal – Wallis test (p≤0.05) using coefficient χ2. The results showed a single legality of the distribution of Cd, Ni and As in samples of soil profile, while the average soil samples showed unique legality of the distribution of observed elements.

Introduction: Surgical and medical treatments of nasal obstruction are a common parts of otolaryngologist practice. The definitive treatment of deviated nasal septum is septoplasty. Aim: In this study was to evaluate the values of subjective parameters, and active anterior rhinomanometry parameters prior and three months after the septoplasty. Patients and Methods: We analyzed the subjective parameters (“NOSE” scale), the active anterior rhinomanometry parameters according to International Committee on Standardization of Rhinomanometry, on 40 patients. Thirty healthy adult volunteers participated belonged to the control group. None of the patients or healthy volunteers had previous history of nasal surgery or active rhinological disease. Results: The post-operative improvement in symptoms of nasal obstruction obtained in 92,5% patients and improvement parameters of the active anterior rhinomanometry in 42,5% patients. Conclusion: The correlation between the findings with rhinomanometry and subjective sensation of nasal patency remains uncertain. There still seems to be only a limited argument for the use of rhinomanometry for quantifying surgical results. Three months postoperative findings are very early results to interpret the permanent effects.

Targeted immunotherapy based on PD-1/PD-L1 suppression has revolutionized the treatment of various solid tumors. A remarkable improvement has also been observed in the treatment of patients with refractory/relapsing classical Hodgkin lymphoma (cHL). We investigated PD-L1 status in a variety of treatment resistant lymphomas. Tumor samples from 78 patients with therapy resistant lymphomas were immunohistochemically (IHC) investigated for the expression of PD-L1 using two antibody clones (SP142 and SP263, Ventana). Thirteen PD-L1+ cases were further analyzed for gene copy number variations (CNV) by NGS and for PD-L1/JAK2/PD-L2 co-amplification using fluorescent in-situ hybridization assay (FISH). PD-L1 positivity (≥5% positive cancer cells, IHC) was present in 32/77 (42%) and 33/71 cases (46%) using SP142 and SP263 antibodies, respectively. Concordance between the two anti-PD-L1 clones was high with only three (4%) discrepant cases. The strongest and consistent (10/11 cases) expression was observed in cHL and primary mediastinal B-cell lymphomas (3/3). Diffuse large B-cell lymphomas (DLBCL) were frequently positive (13/26) irrespective of subtype. Follicular (1/8), peripheral T-cell (3/11) and mantle cell (1/8) lymphomas were rarely positive, while small lymphocytic lymphoma/CLL and marginal zone lymphomas were consistently negative (3/3). Co-amplification/CNVs of PD-L1/JAK2/PD-L2 were observed in 3 cases of DLBCL and cHL, respectively. Of note, all three cHL-amplified cases were positive by FISH, but not by NGS. Since only a fraction of the IHC positive lymphoma cases were positive by FISH and NGS assays, other mechanisms are involved in PD-L1 upregulation, especially in DLBCL. FISH assay may be more suitable than NGS assay for determination of PD-L1 alterations in cHL.

UDK 631.164(497.6) The main act to consider the value of land from the perspective of various sectors and their needs (urban planning, agriculture etc.) in the Federation of B&H is the Decree on unique methodology for preparation of the spatial planning documents (Official Gazette of the Federation of B&H, No. 63/04 and No. 50/07), which prescribes the obligation of making the Land capability study as a segment of spatial basis. Land capability study projects are being implemented by the Institute of Soil, Agro chemistry and Melioration (PAM) of the Faculty of Agricultural and Food Sciences University of Sarajevo. By using defined soil bonity categories, land capability study defines the natural potential of soils in terms of agricultural production and food production, and defines the zones for various land use types. From the rational land use point of view, it means adequate spatial planning, urban development and environmental protection. This paper aims to present the importance, characteristics as well as use of the Land capability study at the municipality level using municipalities of Tuzla Canton as example. Agricultural areas of on the territory of five municipalities in Tuzla Canton (Gradacac, Gracanica, Kalesija, Zivinice, Sapna) account for 44,356.1 ha or 42.6% of the total area. Represented in these areas are soil bonity categories that range from II to VII. The first four bonity categories that are determined exclusively as agricultural land account for 28,739.91 ha or 63.1% of the total area. Within this group, the most represented is the IVb category with 17,229.8 ha. The best quality bonity categories II and III account for 7,514.3 ha or 16.5% of the total area. Analysis of the orthophoto images has identified a significant dynamics of change in space in the period 2008-2012 in four municipalities (Gradacac, Kalesija, Zivinice, Gracanica). Analysis has established that artificial areas (developed) increased by 737 ha, agricultural areas without orchards decreased by 1,136.72 ha, while the areas under orchards increased by 283.13 ha. The largest expansion of developed areas and settlements was recorded within the bonity categories IVb (47% of the total change) and III (27%).

UDK 631.4:551.583(497.6) The value of the landscape in the karst region of Bosnia and Herzegovina (B&H) is the foundation of its existence and the key role in its appearance and formation is played by geomorphology and the soil. Through history the man has created a „cultural landscape” which is completely adapted to the natural conditions. He further enriches the space and makes it more appealing. Soils in B&H karst are extremely heterogeneous and form a real pedological mosaic. Found on the Mesozoic sediments of limestone and dolomite of the Middle and Upper Jurassic and Early and Late Cretaceous are Bare rocks (barren land), Rocky grounds (Lithosol), Limestone-dolomite Black soil (Calcomelanosol) and Brown soil on limestone and dolomite (Calcocambisol). A specificity of these rocks are the screes that are transported down the hillside. A series may occur on them in which, if shale materials are contained, Rendzina appears as a calcareous soil. In addition to the above mentioned limestone and dolomite, there are marly limestones of Jurassic and Cretaceous age and Quaternary sediments on which Alluvial soils (Fluvisol) developed as well as the soils of karst fields which are sometimes very porous and skeletal and sometimes heavy and clayey on impermeable substrate (hydromorphic soils). The paper will provide an overview of characteristic soil types in the Sub-Mediterranean upper and lower karst region of B&H taking into account a range of properties that make them sensitive and vulnerable within the ecosystem. These should include a lack of water on the surface and large fluctuations in the amount of water during the rainy and dry seasons and whimsicality of climate in general, which has a huge impact on the state of biodiversity and human lives in this region.

UDK 631.4:551.435.8(497.6) Natural characteristics of karst areas (lack of water on the surface, scarce soil and vegetation) are rather specific and because of them they are often viewed as unsuitable for human habitation. They are often described as inhospitable and passive areas, and Aley (1992) states that karst areas in America are correlated with areas of rural poverty. A large part of Bosnia and Herzegovina consists of karst areas (karst land extending northwest-southeast) which on average are quite sparsely populated. The scarcity of nature, and especially of soil functions in terms of agriculture, as well as the previous period of industrial development caused depopulation which became particularly apparent after the recent war. This contributed to the fact that in some karst areas population has been drastically reduced, in some places by more than 60%, which affects the condition of land cover and land use as well as change in functions of soil in the ecosystem. Given the fact that the soil formation process on karst terrain is very slow (these are mainly shallow and skeletal soils) and in view of the importance of soil and its multi- functionality, this paper is aimed at analyzing the extent and nature of changes which occurred in land cover and in land use in this part of BiH, namely at analyzing the causes and effects. In order to be able to make such analysis, we used land cover databases, aerial photographs as well as orthophoto maps of the terrain.