Table of contentsWORKSHOP 4: Challenging clinical scenarios (CS01–CS06)CS01 Bullous lesions in two children: solitary mastocytomaS. Tolga Yavuz, Ozan Koc, Ali Gungor, Faysal GokCS02 Multi-System Allergy (MSA) of cystic fibrosis: our institutional experienceJessica Hawley, Christopher O’Brien, Matthew Thomas, Malcolm Brodlie, Louise MichaelisCS03 Cold urticaria in pediatric age: an invisible cause for severe reactionsInês Mota, Ângela Gaspar, Susana Piedade, Graça Sampaio, José Geraldo Dias, Miguel Paiva, Mário Morais-AlmeidaCS04 Angioedema with C1 inhibitor deficiency in a girl: a challenge diagnosisCristina Madureira, Tânia Lopes, Susana Lopes, Filipa Almeida, Alexandra Sequeira, Fernanda Carvalho, José OliveiraCS05 A child with unusual multiple organ allergy disease: what is the primer?Fabienne Gay-CrosierCS06 A case of uncontrolled asthma in a 6-year-old patientIoana-Valentina Nenciu, Andreia Florina Nita, Alexandru Ulmeanu, Dumitru Oraseanu, Carmen ZapucioiuORAL ABSTRACT SESSION 1: Food allergy (OP01–OP06)OP01 Food protein-induced enterocolitis syndrome: oral food challenge outcomes for tolerance evaluation in a Pediatric HospitalAdrianna Machinena, Olga Domínguez Sánchez, Montserrat Alvaro Lozano, Rosa Jimenez Feijoo, Jaime Lozano Blasco, Mònica Piquer Gibert, Mª Teresa Giner Muñoz, Marcia Dias da Costa, Ana Maria Plaza MartínOP02 Characteristics of infants with food protein-induced enterocolitis syndrome and allergic proctocolitisEbru Arik Yilmaz, Özlem Cavkaytar, Betul Buyuktiryaki, Ozge Soyer, Cansin SackesenOP03 The clinical and immunological outcomes after consumption of baked egg by 1–5 year old egg allergic children: results of a randomised controlled trialMerrynNetting, Adaweyah El-Merhibi, Michael Gold, PatrickQuinn, IrmeliPenttila, Maria MakridesOP04 Oral immunotherapy for treatment of egg allergy using low allergenic, hydrolysed eggStavroula Giavi, Antonella Muraro, Roger Lauener, Annick Mercenier, Eugen Bersuch, Isabella M. Montagner, Maria Passioti, Nicolò Celegato, Selina Summermatter, Sophie Nutten, Tristan Bourdeau, Yvonne M. Vissers, Nikolaos G. PapadopoulosOP05 Chemical modification of a peanut extract results in an increased safety profile while maintaining efficacyHanneke van der Kleij, Hans Warmenhoven, Ronald van Ree, Raymond Pieters, Dirk Jan Opstelten, Hans van Schijndel, Joost SmitOP06 Administration of the yellow fever vaccine in egg allergic childrenRoisin Fitzsimons, Victoria Timms, George Du ToitORAL ABSTRACT SESSION 2: Asthma (OP07–OP12)OP07 Previous exacerbation is the most important risk factor for future exacerbations in school-age children with asthmaS. Tolga Yavuz, Guven Kaya, Mustafa Gulec, Mehmet Saldir, Osman Sener, Faysal GokOP08 Comparative study of degree of severity and laboratory changes between asthmatic children using different acupuncture modalitiesNagwa Hassan, Hala Shaaban, Hazem El-Hariri, Ahmed Kamel Inas E. MahfouzOP09 The concentration of exhaled carbon monoxide in asthmatic children with different controlled stadiumPapp Gabor, Biro Gabor, Kovacs CsabaOP10 Effect of vitamin D3 supplementation during pregnancy on risk of persistent wheeze in the offspring: a randomised clinical trialBo Chawes, Klaus Bønnelykke, Jakob Stokholm, Lene Heickendorff, Susanne Brix, Morten Rasmussen, Hans BisgaardOP11 Lung function development in childhoodHenrik Wegener Hallas, Bo Chawes, Lambang Arianto, Hans BisgaardOP12 Is the effect of maternal and paternal asthma different in female and male children before puberty?Maike Pincus, Thomas Keil, Andreas Reich, Ulrich Wahn, Susanne Lau, Linus GrabenhenrichORAL ABSTRACT SESSION 3: Epidemiology—genetics (OP13–OP18)OP13 Lifestyle is associated with incidence and category of allergen sensitisation: the ALADDIN birth cohortSara Fagerstedt, Helena Marell Hesla, Emelie Johansson, Helen Rosenlund, Axel Mie, Annika Scheynius, Johan AlmOP15 Maternal filaggrin mutations increase the risk of atopic dermatitis in children: an effect independent of mutation inheritanceJorge Esparza-Gordillo, Anja Matanovic, Ingo Marenholz, Anja Bauerfeind, Klaus Rohde, Katja Nemat, Min-Ae Lee-Kirsch, Magnus Nordenskjöld, Marten C. G. Winge, Thomas Keil, Renate Krüger, Susanne Lau, Kirsten Beyer, Birgit Kalb, Bodo Niggemann, Norbert Hübner, Heather J. Cordell, Maria Bradley, Young-Ae LeeOP16 Allergic multimorbidity of asthma, rhinitis and eczema in the first 2 decades of the German MAS birth cohortThomas Keil, Hannah Gough, Linus Grabenhenrich, Dirk Schramm, Andreas Reich, John Beschorner, Antje Schuster, Carl-Peter Bauer, Johannes Forster, Fred Zepp, Young-Ae Lee, Renate Bergmann, Karl Bergmann, Ulrich Wahn, Susanne LauOP17 Childhood anaphylaxis: a growing concernFilipe Benito Garcia, Inês Mota, Susana Piedade, Ângela Gaspar, Natacha Santos, Helena Pité, Mário Morais-AlmeidaOP18 Indoor exposure to molds and dampness in infancy and its association to persistent atopic dermatitis in school age. Results from the Greek ISAAC II studyAthina Papadopoulou, Despina Mermiri, Elpida Xatziagorou, Ioannis Tsanakas, Stavroula Lampidi, Kostas PriftisORAL ABSTRACT SESSION 4: Pediatric rhinitis—immunotherapy (OP19–OP24)OP19 Associations between residential greenness and childhood allergic rhinitis and aeroallergen sensitisation in seven birth cohortsElaine Fuertes, Iana Markevych, Gayan Bowatte, Olena Gruzieva, Ulrike Gehring, Allan Becker, Dietrich Berdel, Michael Brauer, Chris Carlsten, Barbara Hoffmann, Anita Kozyrskyj, Caroline Lodge, Göran Pershagen, Alet Wijga, Heinrich JoachimOP20 Full symptom control in pediatric patients with allergic rhinitis and asthma: results of a 2-year sublingual allergen immunotherapy studyZorica Zivkovic, Ivana Djuric-Filipovic, Jasmina Jocić-Stevanovic, Snežana ZivanovicOP21 Nasal epithelium of different ages of atopic subjects present increased levels of oxidative stress and increased cell cytotoxicity upon rhinovirus infectionStyliani Taka, Dimitra Kokkinou, Aliki Papakonstantinou, Panagiota Stefanopoulou, Anastasia Georgountzou, Paraskevi Maggina, Sofia Stamataki, Vassiliki Papaevanggelou, Evangelos Andreakos, Nikolaos G. PapadopoulosOP22 Cluster subcutaneous immunotherapy schedule: tolerability profile in childrenMonica Piquer Gibert, Montserrat Alvaro Lozano, Jaime Lozano Blasco, Olga Domínguez Sánchez, Rosa Jiménez Feijoo, Marcia Dias da Costa, Mª Teresa Giner Muñoz, Adriana Machinena Spera, Ana Maria Plaza MartínOP23 Rhinitis as a risk factor for asthma severity in 11-year old children: population-based cohort studyMatea Deliu, Danielle Belgrave, Angela Simpson, Adnan CustovicOP24 The Global Lung Function Initiative equations in airway obstruction evaluation of asthmatic childrenJoão Gaspar Marques, Pedro Carreiro-Martins, Joana Belo, Sara Serranho, Isabel Peralta, Nuno Neuparth, Paula Leiria-PintoPOSTER DISCUSSION SESSION 1: Food allergy (PD01–PD05)PD01 Allergen-specific humoral and cellular responses in children who fail egg oral immunotherapy due to allergic reactionsMarta Vazquez-Ortiz, Mariona Pascal, Ana Maria Plaza, Manel JuanPD02 FoxP3 epigenetic features in children with cow milk allergyLorella Paparo, Rita Nocerino, Rosita Aitoro, Ilaria Langella, Antonio Amoroso, Alessia Amoroso, Carmen Di Scala, Roberto Berni CananiPD04 Combined milk and egg allergy in early childhood: let them eat cake?Santanu Maity, Giuseppina Rotiroti, Minal GandhiPD05 Introduction of complementary foods in relation to allergy and gut microbiota in farm and non-farm childrenKarin Jonsson, Annika Ljung, Bill Hesselmar, Ingegerd Adlerbert, Hilde Brekke, Susanne Johansen, Agnes Wold, Ann-Sofie SandbergPOSTER DISCUSSION SESSION 2: Asthma and wheeze (PD06–PD16)PD06 The association between asthma and exhaled nitric oxide is influenced by genetics and sensitisationBjörn Nordlund, Cecilia Lundholm, Villhelmina Ullemar, Marianne van Hage, Anne Örtqvist, Catarina AlmqvistPD09 Prevalence patterns of infant wheeze across EuropeAnna Selby, Kate Grimshaw, Thomas Keil, Linus Grabenhenrich, Michael Clausen, Ruta Dubakiene, Alessandro Fiocchi, Marek Kowalski, Nikos Papadopoulos, Marta Reche, Sigurveig Sigurdardottir, Aline Sprikkleman, Paraskevi Xepapadaki, Clare Mills, Kirsten Beyer, Graham RobertsPD10 Epidemiologic changes in recurrent wheezing infantsHerberto Jose Chong Neto, Gustavo Falbo Wandalsen, Ana Carolina Dela Bianca, Carolina Aranda, Nelson Augusto Rosário, Dirceu Solé, Javier Mallol, Luis García MarcosPD13 A single nucleotide polymorphism in the GLCCI1 gene is associated with response to asthma treatment in childrenIvanaBanic, Matija Rijavec, Davor Plavec, Peter Korosec, Mirjana TurkaljPD14 Pollen induced asthma: Could small molecules in pollen exacerbate the protein-mediated allergic response?Alen Bozicevic, Maria De Mieri, Matthias HamburgerPD15 A qualitative study to understand how we can empower teenagers to better self-manage their asthmaSimone Holley, Ruth Morris, Frances Mitchell, Rebecca Knibb, Susan Latter, Christina Liossi, Graham RobertsPD16 Polymorphism of endothelial nitric oxide synthase (eNOS) gene among Egyptian children with bronchial asthmaMostafa M. M. HassanPOSTER DISCUSSION SESSION 3: Mechanisms—Epidemiology (PD17–PD21)PD17 Pregnancy outcomes in relation to development of allergy in a Swedish birth cohortMalin Barman, Anna Sandin, Agnes Wold, Ann-Sofie SandbergPD18 Evolution of the IgE response to house dust mite molecules in childhoodDaniela Posa, Serena Perna, Carl-Peter Bauer, Ute Hoffmann, Johannes Forster, Fred Zepp, Antje Schuster, Ulrich Wahn, Thomas Keil, Susanne Lau, Kuan-Wei Chen, Yvonne Resch, Susanne Vrtala, Rudolf Valenta, Paolo Maria MatricardiPD19 Antibody recognition of nsLTP-molecules as antigens but not as allergens in the German-MAS birth cohortOlympia Tsilochristou, Alexander Rohrbach, Antonio Cappella, Stephanie Hofmaier, Laura Hatzler, Carl-Peter Bauer, Ute Hoffmann, Johannes Forster, Fred Zepp, Antje Schuster, RaffaeleD’Amelio, Ulrich Wahn, Thomas Keil, Susanne Lau, Paolo Maria MatricardiPD20 Early life coloniza

Using the X-ray standing wave method, scanning tunneling microscopy, low energy electron diffraction, and density functional theory, we precisely determine the lateral and vertical structure of hexagonal boron nitride on Ir(111). The moiré superstructure leads to a periodic arrangement of strongly chemisorbed valleys in an otherwise rather flat, weakly physisorbed plane. The best commensurate approximation of the moiré unit cell is (12 × 12) boron nitride cells resting on (11 × 11) substrate cells, which is at variance with several earlier studies. We uncover the existence of two fundamentally different mechanisms of layer formation for hexagonal boron nitride, namely, nucleation and growth as opposed to network formation without nucleation. The different pathways are linked to different distributions of rotational domains, and the latter enables selection of a single orientation only.

This paper is related to a research of modelling fuzzy-neural systems using the coevolutionary algorithm, and has the focus on advantages of using the coevolutionary algorithm for system structure optimization. In the context of this work, the term fuzzy-neural system defines the system that can be used as the fuzzy system with all its functionalities or as the neural network with all its functionalities. The hybridization of fuzzy logic, neural networks and coevolutionary algorithm and its architecture are presented in general, and the role of the coevolutionary algorithm in structure optimization is described in details. Results of testing with Iris Database, from UCI Machine Learning Repository are also presented. Tests performed during the research supports the conclusion that usage of the coevolutionary algorithm for the fuzzy-neural system’s structure optimization is very efficient.

PURPOSE: The aim of this study was to examine the reliability, validity and usefulness of the 30–15IFT in competitive female soccer players. METHODS: Seventeen elite female soccer players participated in the study. A within subject test-retest study design was utilized to assess the reliability of the 30–15 intermittent fitness test (IFT). Seven days prior to 30–15IFT, subjects performed a continuous aerobic running test (CT) under laboratory conditions to assess the criterion validity of the 30–15IFT. End running velocity (VCT and VIFT), peak heart rate (HRpeak) and maximal oxygen consumption (VO2max) were collected and/or estimated for both tests. RESULTS: VIFT (ICC = 0.91; CV = 1.8%), HRpeak (ICC = 0.94; CV = 1.2%), and VO2max (ICC = 0.94; CV = 1.6%) obtained from the 30–15IFT were all deemed highly reliable (p > 0.05). Pearson product moment correlations between the CT and 30–15IFT for VO2max, HRpeak and end running velocity were large (r = 0.67, p = 0.013), very large (r = 0.77, p = 0.02) and large (r = 0.57, p = 0.042), respectively. CONCLUSION: Current findings suggest that the 30–15IFT is a valid and reliable intermittent aerobic fitness test of elite female soccer players. The findings have also provided practitioners with evidence to support the accurate detection of meaningful individual changes in VIFT of 0.5 km/h (1 stage) and HRpeak of 2 bpm. This information may assist coaches in monitoring “real” aerobic fitness changes to better inform training of female intermittent team sport athletes. Lastly, coaches could use the 30–15IFT as a practical alternative to laboratory based assessments to assess and monitor intermittent aerobic fitness changes in their athletes.

Heavy metal ATPases (HMAs) are the most important proteins involved in heavy metal accumulation process. Brassica oleracea has 5 HMA (1-5) homologues whose 3D structure has been predicted and validated in this study by different bioinformatics tools. Phylogenetic and multiple sequence alignment analyses showed high relationship between HMA2 and HMA4, while two same domains were identified in all five HMA proteins: E1-E2 ATPase and haloacid dehydrogenase (HAD) domain. Four HMA (2-5) proteins were identified to be localized in the plasma membrane, while HMA1 localization is predicted to be in plastid. Interactome analysis revealed high interaction of all HMA (1-5) proteins with many metal ion binding proteins and chaperones. Among these, interesting and strong interaction is observed between all HMA (1-5) proteins and ATX1, while HMA1, HMA2 and HMA4 have been found to strongly interact with FP3 (farnesylated protein 3) and FP6 (farnesylated protein 6) proteins. Docking site predictions and electrostatic potentials between HMA2/HMA4 and the interactome proteins were explained and discussed in this study.

Nanoparticles are molecules with size depended chemical and pyhsical characteristics, enabling interesting and correlated approaches while dealing with fundamental biological questions. Nanoparticles are capable of strong and important interaction with other molecules. Many different nanoparticles are produced, with variety of different roles, but Gold nanoparticle as metal based beads, have specific importance due to their attractive physical and chemical properties, biocompatibility, and facile surface modification. In general, nanoparticles have the ability to interact with whole physiological surrounding once when they enter human body. In most of the cases, first molecule they interact with are proteins, which are the main constituens of human body and the driving force of most of the biological processes. This understanding of interaction between nanoparticles and proteins represents an important essence for secure and efficient application of nanoparticles. In this regards, several methods for nanoparticle-protein interaction were developed and analysed in this review. Further, this paper reviews the current scientific development in nanoparticle-protein interactions.

In this work we describe the development of the kinetic molecular theory of liquids (KMTL) concept inventory, as well as its use in investigating students’ conceptual understanding of the KMTL within the contexts of aggregate states, evaporation, boiling, condensation, conduction, convection, diffusion, and surface tension. We implemented think-alouds to prepare distractors for the closed-ended version of the KMTL, which was administered to two groups of respondents: non-physicists and physicists (166 students in total from the Universities of Rijeka and Split, Croatia). From the think-alouds and results of written survey research we drew conclusions about the students’ understanding of the structure of matter, thermal internal energy, entropy, temperature, and pressure. Our study not only reiterates earlier findings on students’ ideas about the KMTL, it also reveals numerous additional misconceptions that had not been reported earlier. Psychometric analyses support a formative use of the KMTL inventory. The inventory questions may be extensively used for identifying misconceptions, as well as for stimulating classroom discussions and conceptual change.

Extracurricular activities represent educational, preplanned actions which enable complete confirmation of students’ personality as well as development of their communication skills and competencies; whilst they enable the teachers to expand their educational influence. Modern school can’t be imagined without innovations or modifications to the entire communicational discourse, since its perspective is to be a progressive model whereat main communicational types of behavior are learnt and formed. The research was carried out at primary and secondary schools in the area of Bosnia and Herzegovina (Mostar region) and it included 590 students and 315 teachers. With this empirical research we have tested the presence of a democratic, voluntary, partnership and pedagogic-stimulating communication within the process of putting the extracurricular activities into realization. The goal of our research was to determine whether there is a statistically significant difference between evaluations made by students and those made by teachers of primary and secondary schools that a democratic, voluntary, partnership and pedagogic stimulating communication is mostly dominant in the process of putting extracurricular activities into realization. The results we have collected have shown the difference between students’ and teachers’ perception of dominant communication in realization of extracurricular activities.

A acao repetitiva e o esforco fisico podem provocam acidentes como os cortes, quedas, torcoes, causarem problemas nas colunas e pes, alem de caibras e tendinites. Diante deste cenario, o presente estudo  objetivou analisar os riscos  que ocasionam  acidentes de trabalho numa area de usina de acucar e alcool na Zona da Mata Norte do Estado de Pernambuco.  Foram analisados atraves de pesquisa de campo de carater quantitativa, por amostragem com aplicacao de um questionario, com aspectos investigativos, os tipos de acidentes com maiores frequencias, gravidades e quantos dias que este acidente afastou o trabalhador de sua atividade laboral, para assim, compreender e prevenir esses riscos que se acentuam nos canaviais,. Os trabalhadores recebem os EPI’s, mas nao os usam corretamente, utilizando pedra de esmeril para amolar o facao de forma insegura, causando ferimento na mao, dependendo da gravidade o trabalhador pode ficar em media de 7 a 10 dias afastado. E organizada uma forca tarefa no campo com a participacao dos Gerentes do campo, Tecnicos agricolas liderem de turma e em especial os tecnicos do Servico Especializado em Seguranca do Trabalho Rural – SESTR com a realizacaode treinamentos no canavial, educando e conscientizando cada trabalhador sobre a importância de se proteger. O campo recebe esta visita duas vezes por semana, incluindo a presenca da Enfermeira do Trabalho desta forma o indice de acidente por amolar o facao reduziu em media de 70%.  Case study on risks and accidents among rural workers of a plant marta Pernambuco north Abstract : The repetitive action and physical exertion can cause accidents such as cuts, falls, sprains, causing problems in columns and feet, as well as cramps and tendonitis. In this scenario, this study aimed to analyze the risks that cause workplace accidents in an area of sugar and alcohol plant in the Zona da Mata Norte of Pernambuco. Were analyzed by quantitative character of field research, sample by applying a questionnaire with investigative aspects, types of accidents with higher frequencies, severities and how many days this accident away the worker of his labor activity, thus, understand and prevent these risks are accentuated in the cane fields. Workers receive the PPE, but not use them properly, using emery stone to sharpen the knife in an unsafe manner, causing injury to the hand, depending on the severity worker can get an average of 7 to 10 days away. It organized a task force in the field with the participation of field managers, shift to lead agricultural technicians and especially the staff of the Specialized Service in Rural Work Safety - SESTR with realizacaode training in the cane fields, educating and educating each employee of the importance to protect. The field gets this visit twice a week, including the presence of the Labor Nurse this way the accident rate by sharpening the machete reduced by an average of 70%.