In this study, boroxine derivative (K2[B3O3F4OH]) was tested as an inhibitor of horseradish peroxidase (HRP) by spectrophotometric and electrochemical methods. The activity of horseradish peroxidase was first studied under steady-state kinetic conditions by a spectrophotometric method which required the use of guaiacol as a second substrate to measure guaiacol peroxidation. The results of this method have shown that, by changing the concentration of guaiacol as the literature suggests, a different type of inhibition is observed than when changing the concentration of hydrogen peroxide as the substrate. This suggests that guaiacol interferes with the reaction in some way. The electrochemical method involves direct electron transfer of HRP immobilized in Nafion nanocomposite films on a glassy carbon (GC) electrode, creating a sensor with an electro-catalytic response to the reduction of hydrogen peroxide. The electrochemical method simplifies kinetic assays by removing the requirement of reducing substrates.

This article presents architecture of integrated intelligent computer-aided design system for designing mechanical power-transmitting mechanisms (IICADkmps). The system has been developed in C# program environment with the aim of automatising the design process. This article presents a modern, automated approach to design. Developed kmps modules for calculation of geometrical and design characteristics of mechanical power-transmitting mechanisms are described. Three-dimensional geometrical parameter modelling of mechanical power-transmitting mechanisms was performed in the computer-aided design/computer-aided manufacturing/computer-aided engineering system CATIA V5. The connection between kmps calculation modules and CATIA V5 modelling system was established through initial three-dimensional models – templates. The outputs from the developed IICADkmps system generated final three-dimensional virtual models of mechanical power-transmitting mechanisms. Testing of the developed IICADkmps system was performed on friction, belt, cogged (spur and bevel gears) and chain transmitting mechanisms. Also, connection of the developed IICADkmps system with a device for rapid prototyping and computer numerical control machines was made for the purpose of additional testing and verification of practical use. Physical prototypes of designed characteristic elements of mechanical power-transmitting mechanisms were manufactured. The selected test three-dimensional virtual prototypes, obtained as an output from the developed IICADkmps system, were manufactured on the device for rapid prototyping (three-dimensional colour printer Spectrum Z510) and computer numerical control machines. Finally, at the end of the article, conclusions and suggested possible directions of further research, based on theoretical and practical research results, are presented.

Extracellular vesicles (EVs), such as exosomes and microvesicles, have been identified as mediators of a newly-discovered intercellular communication system. They are essential signaling mediators in various physiological and pathophysiological processes. Depending on their origin, they fulfill different functions. EVs of mesenchymal stem/stromal cells (MSCs) have been found to promote comparable therapeutic activities as MSCs themselves. In a variety of in vivo models, it has been observed that they suppress pro-inflammatory processes and reduce oxidative stress and fibrosis. By switching pro-inflammatory into tolerogenic immune responses, MSC-EVs very likely promote tissue regeneration by creating a pro-regenerative environment allowing endogenous stem and progenitor cells to successfully repair affected tissues. Accordingly, MSC-EVs provide a novel, very promising therapeutic agent, which has already been successfully applied to humans. However, the MSC-EV production process has not been standardized, yet. Indeed, a collection of different protocols has been used for the MSC-EV production, characterization and application. By focusing on kidney, heart, liver and brain injuries, we have reviewed the major outcomes of published MSC-EV in vivo studies.