Abstract The tumour suppressor p53, encoded by TP53, is a key player in a wide network of signalling pathways. We investigated its role in the bioactivation of the environmental carcinogen 3-nitrobenzanthrone (3-NBA)found in diesel exhaust and its metabolites 3-aminobenzanthrone (3-ABA) and N-hydroxy-3-aminobenzanthrone (N-OH-3-ABA) in a panel of isogenic human colorectal HCT116 cells differing only with respect to their TP53 status [i.e. TP53(+/+), TP53(+/−), TP53(−/−), TP53(R248W/+) or TP53(R248W/−)]. As a measure of metabolic competence, DNA adduct formation was determined using 32P-postlabelling. Wild-type (WT) p53 did not affect the bioactivation of 3-NBA; no difference in DNA adduct formation was observed in TP53(+/+), TP53(+/−) and TP53(−/−) cells. Bioactivation of both metabolites 3-ABA and N-OH-3-ABA on the other hand was WT-TP53 dependent. Lower 3-ABA- and N-OH-3-ABA-DNA adduct levels were found in TP53(+/−) and TP53(−/−) cells compared to TP53(+/+) cells, and p53’s impact was attributed to differences in cytochrome P450 (CYP) 1A1 expression for 3-ABA whereas for N-OH-3-ABA, an impact of this tumour suppressor on sulphotransferase (SULT) 1A1/3 expression was detected. Mutant R248W-p53 protein function was similar to or exceeded the ability of WT-p53 in activating 3-NBA and its metabolites, measured as DNA adducts. However, identification of the xenobiotic-metabolising enzyme(s) (XMEs), through which mutant-p53 regulates these responses, proved difficult to decipher. For example, although both mutant cell lines exhibited higher CYP1A1 induction after 3-NBA treatment compared to TP53(+/+) cells, 3-NBA-derived DNA adduct levels were only higher in TP53(R248W/−) cells but not in TP53(R248W/+) cells. Our results show that p53’s influence on carcinogen activation depends on the agent studied and thereby on the XMEs that mediate the bioactivation of that particular compound. The phenomenon of p53 regulating CYP1A1 expression in human cells is consistent with other recent findings; however, this is the first study highlighting the impact of p53 on sulphotransferase-mediated (i.e. SULT1A1) carcinogen metabolism in human cells.

Article history: Received 20 February 2018 Received in revised form 26 April 2018 Accepted 2 May 2018 An evaluation of aerodynamic forces and moments that act on an irregularly shaped body (fragments of HE projectile) moving at high velocities was made, using numerical simulations, analytical models, and CAD methods. Using the results obtained for aerodynamic forces and moments, and known values of body exposed area, aerodynamic drag and lift coefficients were determined for different body orientations and different flow velocity. Analysis of the influence of the body front surface and body slenderness on the position of the maximum CD value (on the CD (Ma) curve) was performed.

Motivation Segmental duplications (SDs) or low‐copy repeats, are segments of DNA > 1 Kbp with high sequence identity that are copied to other regions of the genome. SDs are among the most important sources of evolution, a common cause of genomic structural variation and several are associated with diseases of genomic origin including schizophrenia and autism. Despite their functional importance, SDs present one of the major hurdles for de novo genome assembly due to the ambiguity they cause in building and traversing both state‐of‐the‐art overlap‐layout‐consensus and de Bruijn graphs. This causes SD regions to be misassembled, collapsed into a unique representation, or completely missing from assembled reference genomes for various organisms. In turn, this missing or incorrect information limits our ability to fully understand the evolution and the architecture of the genomes. Despite the essential need to accurately characterize SDs in assemblies, there has been only one tool that was developed for this purpose, called Whole‐Genome Assembly Comparison (WGAC); its primary goal is SD detection. WGAC is comprised of several steps that employ different tools and custom scripts, which makes this strategy difficult and time consuming to use. Thus there is still a need for algorithms to characterize within‐assembly SDs quickly, accurately, and in a user friendly manner. Results Here we introduce SEgmental Duplication Evaluation Framework (SEDEF) to rapidly detect SDs through sophisticated filtering strategies based on Jaccard similarity and local chaining. We show that SEDEF accurately detects SDs while maintaining substantial speed up over WGAC that translates into practical run times of minutes instead of weeks. Notably, our algorithm captures up to 25% ‘pairwise error’ between segments, whereas previous studies focused on only 10%, allowing us to more deeply track the evolutionary history of the genome. Availability and implementation SEDEF is available at https://github.com/vpc‐ccg/sedef.

In this paper, we consider the cooperative system [Formula: see text] where all parameters [Formula: see text] are positive numbers and the initial conditions [Formula: see text] are nonnegative numbers. We describe the global dynamics of this system in a number of cases. An interesting feature of this system is that it exhibits a coexistence of locally stable equilibrium and locally stable periodic solutions as well as the Allee effect.

One of the most important health problems of today's society is hypokinesia accompanied by obesity. The assumption is that reduced physical activity has a large negative impact on the school population. The problem of reduced commitment and involvement of pupils in extracurricular activities is increasingly evident in recent times, which results in a number of negative health effects on the human body of an individual. This study comprised a group of pupils from first to the fourth year of elementary school in Pale (city East Sarajevo, BIH). The total sample consisted of 175 pupils (84 male and 91 female) age 11 to 14±0,5years. As a way of gathering the required information, we used an anonymous questionnaire of the open type in order to collect information about pupils involvement in extracurricular sports activities. The survey was conducted in October 2017 and all the pupils voluntarily participated in the study. Based on the survey results have been obtained the necessary information to reflect the very good of physical activity, of the pupils population. Of the total sample of pupils, the survey confirmed that 100% are physically active pupils. This is an encouraging result because today we have less physically active school children.

<p style="text-align: justify;">PowerPoint is a popular classroom tool to make simple presentations. Such presentations can be flat when creating interactive lessons for students to use while sitting in front of the computers. Using built-in scripting features, Visual Basic for Applications, the interactivity of PowerPoint can be extended to unlimited dimensions. This paper has to aim to introduce basic scripts needed for modeling quiz questions and it is focused on educators<br />with little or no programming background. Quiz questions can be an important part of the e-learning.</p>

SAŽETAK: Iako razne vrste zanimanja različito utječu na ljudsko zdravlje, nepravilni načini držanja tijela i bolovi u leđima povezuju veliki broj radnika i zanimanja. Bolovi u leđima česta su pojava i kod radnika u trgovinama. Njihov posao prvenstveno je okarakteriziran velikim brojem ponavljanja spuštanja i podizanja trupa prilikom slaganja robe. Upravo zato je, u jednom velikom trgovačkom lancu u RH, proveden projekt u području kineziologije rada u obliku sustavnog učenja radnika o pravilnim načinima rukovanja teretima, a s ciljem prevencije bolova u leđima. Ovo istraživanje dio je tog projekta. Uzorak ispitanika čini skupina od 234 zaposlenika koji rade u trgovinama. U sklopu projekta provedene su teorijsko-praktična predavanja na kojima su radnici ispunili anketne upitnike. Anketni upitnici obrađeni su metodama deskriptivne statistike. Utvrđeno je kako 58,02 % ispitanika osjeća bol u donjem dijelu leđa na prvom mjestu, a na prvom, drugom ili trećem mjestu bol u donjem dijelu leđa osjeća 73,11 % ispitanika. Bol u leđima i/ili vratu na prvom mjestu ima 84,90 % ispitanika, a na prvom, drugom i/ili trećem mjestu zajedno bol u leđima i/ili vratu osjeća zapanjujućih 92,45 % ispitanika. Utvrđeno je i kako 86,79 % ispitanika ima automatiziran nepravilan način rukovanja teretima u obliku istaknutog zaobljavanja leđa prilikom rada. Od 100 % ispitanika koji zaobljavaju leđa 94 % ispitanika ima bolove u leđima i/ili vratu, a samo 6 % ispitanika nema bolove u leđima i/ili vratu. Ovo istraživanje sugerira kako nepravilni načini držanja tijela za vrijeme rada fizičkih radnika snažno utječe na pojavu bolova u leđima i vratu. Zaključuje se kako tehnika dizanja tereta s poda nije ništa drugo do motoričko znanje koje se mora naučiti. Međutim, prvo se treba prepoznati potreba sustavnog upoznavanja radnika s pravilnim načinima držanja tijela prilikom rukovanja teretima. Cilj je primarna i sekundarna prevencija bolova u leđima i vratu kao i očuvanje radne sposobnosti radnika.