Purpose: Ventilator associated pneumonia (VAP) is one of the most common hospital-acquired infection in patients hospitalized in intensive care unit (ICU). Aim of this study was to evaluate predictive values of quantitative and qualitative culture of broncholaveolar lavage (BAL) in the diagnosis of VAP comparing with clinical pulmonary infection score (CPIS), and to determine positive and negative predictive values of the tests in patients on mechanical ventilation. Methodology: 209 samples were prospectively taken from the patients hospitalized in ICU on mechanical ventilation; along with the clinical pulmonary infection score (CPIS). After sampling, quantitative and qualitative culture of BAL was done. As the cut off value of quantitative culture 104 CFU/mL was taken, according CDC recommendations. Results: In our study, sensitivity of the quantitative culture of BAL was 91%, specificity 70%, positive predictive value 80% and negative predictive value was 85%. Sensitivity of the qualitative culture of BAL was 93%, specificity 36%, positive predictive value 70,2% and negative predictive value was 76%. Conclusion: Quantitative culture of BAL has better predictive values in VAP diagnosis in patients on mechanical ventilation, helping in the discrimination between colonization and the infection. Qualitative culture of BAL has higher sensitivity, but lower specificity than quantitative culture.

Microbial pathogens secrete small RNA (sRNA) effectors into plant hosts to aid infection by silencing transcripts of immunity and signaling-related genes through RNA interference (RNAi). Similarly, sRNAs from plant hosts have been shown to contribute to plant defense against microbial pathogens by targeting transcripts involved in virulence. This phenomenon is called bidirectional RNA communication or cross kingdom RNAi (ckRNAi). How far this RNAi-mediated mechanism is evolutionarily conserved is the subject of controversial discussions. We examined the bidirectional accumulation of sRNAs in the interaction of the hemibiotrophic rice blast fungus Magnaporthe oryzae (Mo) with the grass model plant Brachypodium distachyon (Bd). By comparative deep sequencing of sRNAs and mRNAs from axenic fungal cultures and infected leaves and roots, we found a wide range of fungal sRNAs that accumulated exclusively in infected tissues. Amongst those, 20-21 nt candidate sRNA effectors were predicted in silico by selecting those Mo reads that had complementary mRNA targets in Bd. Many of those mRNAs predicted to be targeted by Mo sRNAs were differentially expressed, particularly in the necrotrophic infection phase, including gene transcripts involved in plant defense responses and signaling. Vice versa, by applying the same strategy to identify Bd sRNA effectors, we found that Bd produced sRNAs targeting a variety of fungal transcripts, encoding fungal cell wall components, virulence genes and transcription factors. Consistent with function as effectors of these Bd sRNAs, their predicted fungal targets were significantly down-regulated in the infected tissues compared to axenic cultures, and deletion mutants for some of these target genes showed heavily impaired virulence phenotypes. Overall, this study provides the first experimentally-based evidence for bidirectional ckRNAi in a grass-fungal pathosystem, paving the way for further validation of identified sRNA-target duplexes and contributing to the emerging research on naturally occurring cross-kingdom communication and its implications for agriculture on staple crops. Author Summary In the present work, we provide first experimental evidence for bidirectional RNA communication in a grass-fungal pathosystem. We deployed the monocotyledonous plant Brachypodium distachyon, which is a genetic model for the staple crops wheat and rice, to investigate the interaction-related sRNAs for their role in RNA communication. By applying a previously published bioinformatics pipeline for the detection of sRNA effectors we identified potential plant targets for fungal sRNAs and vice versa, fungal targets for plant sRNAs. Inspection of the respective targets confirmed their downregulation in infected relative to uninfected tissues and fungal axenic cultures, respectively. By focusing on potential fungal targets, we identified several genes encoding fungal cell wall components, virulence proteins and transcription factors. The deletion of those fungal targets has already been shown to produce disordered virulence phenotypes. Our findings establish the basis for further validation of identified sRNA-mRNA target duplexes and contribute to the emerging research on naturally occurring cross-kingdom communication and its implications for agriculture.

Remote photoplethysmography proved useful as a non-contact method for estimating physiological data from different parts of the body. Recent studies have mainly focused on the face region for extraction of blood pulsation signal. In this paper, we have demonstrated the feasibility of non-contact remote photoplethysmography in the monitoring of free flap tissue. An experimental study was conducted, where video recordings of free flaps are obtained during breast reconstruction surgery for 8 patients. The hemoglobin absorption rate of the free flap is closely related to signal-to-noise ratio (SNR) values extracted from free flap pulsation signal during surgery. Obtained results show significantly lower SNR values when the free flap is disconnected from the blood supply compared to the SNR values when the flap is intact or after successful blood supply establishment. This method shows potential as a convenient, non-invasive and reliable tool in post-operative microvascular free flap monitoring.

Measles are a highly contagious and communicable viral disease which may be prevented by a sustained vaccination program. Due to missed vaccination, two major epidemics of measles (1997–1999 and 2014–2015) have been recorded after the war in Bosnia and Herzegovina (BH) with over 10,000 patients registered. According to the World Health Organization, BH is categorized as a country with endemic transmission of measles. The last measles epidemic was between 2014 and 2015, with 5,083 documented patients in the Federation of BH. In the first four months of 2019, more than 700 measles cases were registered in the same region. Significant transmission rate has been observed in Sarajevo Canton (SC) with 570 documented measles cases. Out of 570 measles cases in SC, 92.5% were unvaccinated. The most affected were children up to 6 years of age (62.8%), with one documented case of death (7-month old infant). In addition to this report, we discussed key stakeholders and possible circumstances responsible for the epidemic. The measles epidemic is still ongoing.

According to the oral tradition, four to five degrees of kinship back, at the locality of the village of Batočići, the hamlet of Bare, the municipality of Čajnice – Republic of Srpska in BiH, there was an appearance of a tree with atypical sympodial graft in the spiral or alternating position of annual growth with a different tendency of negative heliotropism. This tree reached a height of up to 20 m and dried at the age of about 170 years. According to the oral tradition, after a longer period, between two and three degrees of kinship backward, the appearance of new single beech trees with the same characteristics in the formation of habit was observed. These new trees appeared successively in a relatively narrow space around the first tree, which for this reason was called the "Mother of Fairy Beech". Morphological characteristics of tree habit in this beech population conform with the variety of "tortuous beech" Fagus sylvatica var. – tortuosa, as well partly conforming to the variety of "weeping beech" Fagus sylvatica var. pendula. In this research, the question of inheritance of irregular sympodial branching with a tendency of negative heliotropism in the population of "Fairy Beech from Čajniče" has been raised, regardless of the characterization of the varieties. Thus, in the population of Fairy Beech from Čajniče, which makes about 40 trees in relatively close surroundings, four trees aged over 30 years have been identified in order to determine whether their tree form is reproduced by vegetative cloning and generatively, by seed. The research was conducted in the period from 2014 to 2016 at the Institute for Genetic Resources of the University of Banja Luka. The results show that the form of habit of all four parent trees are conveyed by branching as well as by seed, which proves that there was a mutation of the first tree, i.e. the mother of Fairy Beech from Čajniče, which is transmitted to the offspring.

The aim of this paper is to present the application of quantitative methods in the analysis of foreign trade indicators of corn in Bosnia and Herzegovina. Subsequently, based on the analysis, the forecast of import and export parameters was created for the 2018-20 period which predicted that corn imports and exports would increase both in the natural and value form. Furthermore, it was established for the observed period that Serbia was the largest importer of this crop to Bosnia and Herzegovina, and that Turkey was a country to which the corn from Bosnia and Herzegovina was mostly exported. The research in this paper can serve the purpose of further planning and development of both the production and the markets of this crop and agriculture as a whole.

U ovom radu sintetisana je serija segmentiranih poliuretanskih materijala primenom dverazličite vrste alifatičnih polikarbonatnih diola, oznaka T5651 (50% pentanskih i 50%heksanskih grupa kao ponavljajućih jedinica) i T4671 (30% butanskih i 70% heksanskihgrupa kao ponavljajućih jedinica u strukturi lanca). Segmentirani termoplastični poliuretanskimaterijali su ojačani nanočesticama glina sa slojevitom strukturom (montmorilonit ibentonit). Ispitivan je uticaj udela tvrdih segmenata i nanopunila na mehanička svojstva iapsolutno izduženje segmentiranih poliuretanskih elastomera i hibridnih materijala. Utvrđenoje da udeo tvrdih segmenata kod uzoraka na osnovu polikarbonatnog diola oznake T5651 neutiče na prekidnu čvrstoću, ali ima značajan uticaj na prekidnu čvrstoću elastomerastrukturiranih primenom polikarbonatne komponente oznake T4671. Smanjenje udela tvrdihsegmenata u znatnoj meri povećava prekidno izduženje. Dodatak 1 mas.% nanočesticaorganski modifikovanog bentonita utiče na znatno povećanje vrednosti prekidne čvrstoće iprekidnog izduženja poliuretanskih elastomera sa polikarbonatnom komponentom oznakeT5651, kao mekim segmentom (od 23.5 do 46.7 MPa i od 420 do 702%, redom). Elastomeristrukturirani primenom diola oznake T4671 i ojačani nanočesticama montmorilonita ibentonita pokazuju pogoršana mehanička svojstva u odnosu na neojačane poliuretane saistom vrstom polikarbonatne komponente kao mekog segmenta. Veću sposobnost izduženjapokazuju poliuretanski materijali strukturirani primenom polikarbonatnog prekursora oznakeT5651, usled veće pravilnosti u strukturi lanca polikarbonatnog diola koji sadrži isti brojpentanskih i heksanskih grupa.

Background Because nearly 23,000 more neurosurgeons are needed globally to address 5 million essential neurosurgical cases that go untreated each year, there is an increasing interest in task-shifting and task-sharing (TS/S), delegating neurosurgical tasks to nonspecialists, particularly in low- and middle-income countries (LMICs). This global survey aimed to provide a cross-sectional understanding of the prevalence and structure of current neurosurgical TS/S practices in LMICs. Methods The survey was distributed to a convenience sample of individuals providing neurosurgical care in LMICs with a Web-based survey link via electronic mailing lists of continental societies and various neurosurgical groups, conference announcements, e-mailing lists, and social media platforms. Country-level data were analyzed by descriptive statistics. Results The survey yielded 127 responses from 47 LMICs; 20 countries (42.6%) reported ongoing TS/S. Most TS/S procedures involved emergency interventions, the top 3 being burr holes, craniotomy for hematoma evacuation, and external ventricular drain. Most (65.0%) believed that their Ministry of Health does not endorse TS/S (24.0% unsure), and only 11% believed that TS/S training was structured. There were few opportunities for TS/S providers to continue medical education (11.6%) or maintenance of certification (9.4%, or receive remuneration (4.2%). Conclusions TS/S is ongoing in many LMICs without substantial structure or oversight, which is concerning for patient safety. These data invite future clinical outcomes studies to assess effectiveness and discussions on policy recommendations such as standardized curricula, certification protocols, specialist oversight, and referral networks to increase the level of TS/S care and to continue to increase the specialist workforce.