Autobioassay can be used in analyses of drug effects in two ways. It can be used as an instrument or as a place of drug activity. In the first case it can replace a lacking apparatus and in the second case it can show the effect of drugs that could not otherwise be recorded. By autobioassay on isolated organs an analysis of exogenously added drugs is meant with a simultaneous stimulation of pertaining motor nerves. By applying autobioassay with isolated tissues vivisection can be avoided, especially the one done by repetition, to satisfy certain standards. The described IIO can be used in analyses of neurotransmission and cotransmission. Autobioassay can be used in the quality control of drug effects.

The isolated human ureter obtained of a healthy donor after the kidney transplantation were used as a model-system. It was used for the evaluation of the effect of various substances on the neuromuscular transmission. The piece of the isolated human ureter was long 2—3 cm and was set up in an isolated organ bath in Tyrode solution. In the ureter platinum electrode was introduced and another electrode was in the organ bath. The ureter was stimulated transmurally with the constant stimulant in the constant one min. intervals. Acetylcholine usually decreased the height of the induced contractions. Physostigmine decreased contraction and in some ureter caused block of the effect of the electrical stimulations. Atropine had no effect on the height of the contractions, so the preparation could be considered as atropine resistant organ. Noradrenaline, histamine and serotonin increased the contractions. Aminophylline and morphine decreased the contractions. It is possible that there are unknown neurotransmitters in human ureter that induced contractions and relaxation. Isolated human ureter electrically stimulated in vitro could be used as a model-system for the pharmacological and toxicological investigations.

Isolated and innervated organs from the mouth were prepared. They were striated muscles from the tongue and from the farinx. They were prepared with the motor nerve and set up in an isolated organ bath. The induced contractions were recorded and the xenobiotics-ecotoxins were injected. The toxins were benzene and derivatives, trichlorethylene, mercury and lead. It was found that they increase  the effect of nerve stimulation, they increased the height of induced contractions, depending on concentration. The explanation of the effect was that they inhibit enzymes and they free the neurotransmitters.