Simple Summary Fascin, an actin-binding protein, is upregulated in different types of human cancers. It is reportedly responsible for increasing the invasive and metastatic ability of cancer cells by reducing cell–cell adhesions. This review provides a brief overview of fascin and its interactions with other genes and oncoviruses to induce the onset and progression of cancer. Abstract Fascin is an actin-binding protein that is encoded by the FSCN1 gene (located on chromosome 7). It triggers membrane projections and stimulates cell motility in cancer cells. Fascin overexpression has been described in different types of human cancers in which its expression correlated with tumor growth, migration, invasion, and metastasis. Moreover, overexpression of fascin was found in oncovirus-infected cells, such as human papillomaviruses (HPVs) and Epstein-Barr virus (EBV), disrupting the cell–cell adhesion and enhancing cancer progression. Based on these findings, several studies reported fascin as a potential biomarker and a therapeutic target in various cancers. This review provides a brief overview of the FSCN1 role in various cancers with emphasis on gynecological malignancies. We also discuss fascin interactions with other genes and oncoviruses through which it might induce cancer development and progression.

Recurrent Respiratory Papillomatosis (RRP) is a rare but severe manifestation of human papillomavirus (HPV). As our knowledge about HPV infections has expanded, it has become possible to understand the course of RRP disease and unravel plausible efficient methods to manage the disease. However, the surge in reports on HPV has not been accompanied by a similar increase in research about RRP specifically. In this paper, we review the clinical manifestation and typical presentation of the illness. In addition, the pathogenesis and progression of the disease are described. On the other hand, we discuss the types of treatments currently available and future treatment strategies. The role of vaccination in both the prevention and treatment of RRP will also be reviewed. We believe this review is essential to update the general knowledge on RRP with the latest information available to date to enhance our understanding of RRP and its management.

The paper presents the engineering practice, which the company “Regeneracija” Ltd. Velika Kladuša – Bosnia and Herzegovina uses to perform preliminary experimental testing and measurements, followed by mathematical modeling of critical pressure of these vessels, in order to obtain the projected quality of pressure vessels made of composite materials. The paper will confirm the hypothesis that it is possible to relate mathematical connection and dependence of the critical pressure of vessels of composite materials (Pkr) with mechanical characteristics of vessel material (σM), vessel diameter (D), and vessel wall thickness (s). In this way, by varying the mentioned parameters, it is possible to achieve the desired product quality in the production of composite material containers by achieving the projected critical and thus working pressure. Generally speaking, the mathematical model of critical pressure obtained in this way will be a good indicator for design engineers to know how much critical pressure a given vessel can withstand, and based on that to take quick control of working or projected pressure, but also for designing completely new vessels made of composite materials as a substitute for the expensive experimental testing.

This paper investigates the local and global character of the unique positive equilibrium of a mixed monotone fractional second-order difference equation with quadratic terms. The corresponding associated map of the equation decreases in the first variable, and it can be either decreasing or increasing in the second variable depending on the corresponding parametric values. We use the theory of monotone maps to study global dynamics. For local stability, we use the center manifold theory in the case of the non-hyperbolic equilibrium point. We show that the observed equation exhibits three types of global behavior characterized by the existence of the unique positive equilibrium, which can be locally stable, non-hyperbolic when there also exist infinitely many non-hyperbolic and stable minimal period-two solutions, and a saddle. Numerical simulations are carried out to better illustrate the results.

Determination of tensile shear strength of lap joints is carried out according to three standards: BAS EN 205:2018 for adhesives used for non–load–bearing structures, and according to BAS EN 302-1:2014 for adhesives used for the manufacture of load-bearing structures and according to EN 14257:2019 for lap joints who are exposed to elevated temperatures. The paper presents the results of tensile shear strength of lap joints made of solid fir/spruce (Abies alba ssp./Picea abies spp). Tensile shear strength was tested in 4 groups of tests samples. The Group 1 consisted the samples were 7 days in standard atmosphere [20/65]. The Group 2 consisted the samples were previously soaked in water at (20 ± 5) °C, then recondition in standard atmosphere [20/65]. The Group 3 consisted the samples were previously 6 h soaked in boiling water 2 h, then soaked in water at (20 ± 5) °C; the samples tested in the wet state. The Group 4 consisted the samples were previously exposed to heat in a preheated fan oven, at (80 ± 2) °C, for (60 ± 2) min. The test results can be applied for gluing windows, doors, stairs, high-frequency gluing, veneering panels, etc.

Particleboards are used in the manufacture of furniture, as well as the furnishing of structures and prefabricated homes. Their quality dictates where they can be used and how long they can last. The boards’ characteristics deteriorate as they are used. To examine the change-degradation of the properties of the boards are exposed to the effects of the external climate (rain, snow, wind, sun) or regimes of accelerated aging. For this purpose, a degradation test of the properties of wood-based panels was performed, which aims to create a model that will describe the change in bending strength of the mentioned panels. The paper presents the results of bending strength and thickness swelling tests of 16 mm thick particleboards that were exposed to accelerated aging regimes, and the basic model of property degradation.

In the second half of the 20th century, the industrialisation and deagrarization of Bosnia and Herzegovina had a strong impact on the dynamics of urban development and economic growth of the post-war Sarajevo, which intensified immigration from its relatively underdeveloped regional environment. This was accompanied by accelerated housing construction, and it encouraged the spatial expansion of the city. Planning guidelines were set by the city administration and were based on the long-term development plans. They identified the disposition of urban functions necessary for housing, work, recreation and traffic, and the policy of building multi-residential buildings was aimed general social interest. At the same time, the planning activities neglected the actual socio-economic status of immigrants who had lesser opportunities for housing through the social distribution system of apartments, began the process of self-organized unregulated settlement construction with single-family houses on the city's slopes. This began an era of two parallel but controversial actions within town space: planned and unregulated housing construction. Spontaneous possession of the city's territory with unregulated construction today is characterised by: complex property-legal relations, high degree of construction, absence of public space, pedestrian communications and service functions, low quality of the infrastructure network, and that settlements are formed on unstable terrains and on active landslides. Since the consequences of the complexity of the situation cannot be addressed through radical urban transformation, we see an alternative in the idea of partial spatial interventions – transformation by method of sanation. Starting with the thesis that construction is always deeply connected to society's understanding of the function of space and the place of man in it, we have opened up a central question, and searching for answers is the basic goal of this paper: Is it possible to solve problems accumulated by decades within Sarajevo's unregulated residential settlements through means of transformation by method of sanation? Or: Can partial spatial interventions improve the overall quality of individual and social life? For the purpose of finding answers, we conducted an analysis of the causes of the formation and genesis of these settlements, as well as a series of problems produced by the accumulation of separate spatial interventions without elementary professional guidance. The results of this analysis showed that the answer to the questions asked can be positive, by establishing a critical relationship with the potential of the space of specific settlement sites, in terms of the degree of functional usability, correlation with utilities and user interactions with the environments they inhabit. We have concluded that it is precisely the potential of individual sites, by logically applying the transformation by method of sanation, will enable dual achievement – the merging the solution within the technical and structural aspect of potential landslides with the articulation of the public on new pedestrian communications. Also, it has been shown that the application of this method enables the typification of technical solutions, functions, contents, activities, urban design, and even the public itself. And this means that the conclusions on the characteristics of individual Sarajevo unregulated residential settlements, endangered by landslides, can offer general guidelines for design concepts, within them, an overall improvement of individual and social life.

Natural disasters that cause tremendous material harm and threaten human lives, infrastructure, and economic and social activities become more frequent. While most material and human casualties are the effects of sudden and brief natural disasters, it is not possible to disregard long-term disasters, most frequently triggered by climate change. Damage from long-term disasters is quite significant and hampers the sustainability of the economy and life in densely populated cities. Following climate change, it is concluded that special attention should be paid to coastal cities, cities along rivers and the potential risks of possible disasters should be reduced before they materialize. The reduction of disaster risk is an endeavor involving various professional fields, viewpoints, and stakeholders. An integrated approach that brings together all the necessary elements is therefore required. The planning for protection against natural disasters and in the very moments when it occurs, it is necessary to quickly obtain reliable data to coordinate activities in the field well. However, there are problems in the field with the availability, access, and spatial data use. In this sense, the Spatial Data Infrastructure (SDI) can provide an appropriate framework for sharing spatial data through the cooperation of administration, public, and private institutions and citizens.

Abstract Male infertility is caused by spermatogenetic failure, clinically noted as oligoor azoospermia. Approximately 20% of infertile patients carry a genetic defect. The most frequent genetic defect leading to azoospermia (or severe oligozoospermia) is Klinefelter syndrome (47, XXY), which is numerical chromosomal abnormality and Y- structural chromosome aberration. The human X chromosome is the most stable of all human chromosomes. The X chromosome is loaded with regions of acquired, rapidly evolving genes. The X chromosome may actually play an essential role in male infertility and sperm production. Here we will describe X chromosome aberrations, which are associated with male infertility.

Ex-vivo expanded tumour infiltrating lymphocytes (TIL) show promise in delivering durable responses among several solid tumour indications. However, characterising, quantifying and tracking the active component of TIL therapy remains challenging as the expansion process does not distinguish between tumour reactive and bystander T-cells. Achilles Therapeutics has developed ATL001, a patient-specific TIL-based product, manufactured using the VELOS™ process that specifically targets clonal neoantigens present in all tumour cells within a patient. Two Phase I/IIa clinical trials of ATL001 are ongoing in patients with advanced Non-Small Cell Lung Cancer, CHIRON (NCT04032847), and metastatic or recurrent melanoma, THETIS (NCT03997474). Extensive product characterisation and immune-monitoring are performed through Achilles’ manufacturing and translational science programme. This enables precise quantification and characterisation of the active component of this therapy – Clonal Neoantigen T cells (cNeT) – during manufacture and following patient administration, offering unique insight into the mechanism of action of ATL001 and aiding the development of next generation processes.ATL001 was manufactured using procured tumour and matched whole blood from 8 patients enrolled in the THETIS (n=5) and CHIRON (n=3) clinical trials. Following administration of ATL001, peripheral blood samples were collected up to week 6. The active component of the product was detected via re-stimulation with clonal neoantigen peptide pools and evaluation of IFN-γ and/or TNF-α production. Deconvolution of individual reactivities was achieved via ELISPOT assays. Immune reconstitution was evaluated by flow cytometry. cNeT expansion was evaluated by restimulation of isolated PBMCs with peptide pools and individual peptide reactivities (ELISPOT).The median age was 57 (range 30 – 71) and 6/8 patients were male. The median number of previous lines of systemic anti-cancer treatment at the time of ATL001 dosing was 2.5 (range 1 – 5). Proportion of cNeT in manufactured products ranged from 0.20% - 77.43% (mean 26.78%) and unique single peptide reactivities were observed in 7 of 8 products (range 0 – 28, mean 8.6). Post-dosing, cNeTs were detected in 5/8 patients and cNeT expansion was observed in 3/5 patients.These data underscore our ability to sensitively detect, quantify and track the patient-specific cNeT component of ATL001 – during manufacture and post dosing. As the dataset matures, these metrics of detection and expansion will be correlated with product, clinical and genomic characteristics to determine variables associated with peripheral cNeT dynamics and clinical response.NCT04032847, NCT03997474The first 8 patients described have all been located within the UK and both trials (CHIRON and THETIS) have been approved by the UK MHRA (among other international bodies, e.g FDA). Additionally, these trials have been approved by local ethics boards at active sites within the UK. Patient‘s are fully informed by provided materials and investigators prior to consenting to enrol into either ATL001 trial.

One of the advantages provided by fused deposition modelling (FDM) 3D printing technology is the manufacturing of product materials with infill structure, which provides advantages such as reduced production time, product weight and even the final price. In this paper, the tensile mechanical properties, tensile strength and elastic modulus, of PLA, Tough PLA and PC FDM 3D printed materials with the infill structure were analysed and compared. Also, the influence of infill pattern on tensile properties was analysed. Material testing were performed according to ISO 527-2 standard. All results are statistically analysed and results showed that infill pattern have influence on tensile mechanical properties for all three materials.