ABSTRACT This article sheds light on the status of migrants and asylum seekers in Bosnia and Herzegovina, as well as the state response to the growing challenges in the area of acceptance and protection, following the transformation of the Balkan Migration Route. This shift has caught state-level governmental institutions and local communities unprepared, with high numbers of migrants and refugees stranded upon its territory. A special focus was paid to the Bihać and Velika Kladuša municipalities in Una-Sana Canton, in the northwest of the country, and the ways in which they responded to this situation, in particular the ‘archipelago’ of official and makeshift camps that play a fundamental role in managing migration flows. In order to gain an insight into the problem being analyzed, the methodology applied was primarily based on participant observation and interviews, together with desk research of existing reports and media coverage in different stages of the crisis. The evidence suggests that the unique political infrastructure created after the 1990s has given rise to myriad institutional weaknesses and insufficient coordination between state-level and local authorities in the efforts to prevent a humanitarian emergency.

In this research, the possibility of using Ca(OH)2 in the form of commercial hydrated lime as a catalyst for the methanolysis of refined rapeseed oil was evaluated. Characterization of unused catalyst was performed by SEM-EDS, laser diffraction, XRF, BET, XRD and TG/DTG methods, and vegetable oil was analyzed for physicochemical characteristics. Within methanolysis, the effects of catalyst loading, reaction temperature and reaction time on methyl ester yield, density and viscosity of biodiesel, as well as the possibility of catalyst reuse were investigated. The obtained results showed that hydrated lime is an efficient catalyst for transesterification of vegetable oil, whereby the use of 3 wt% of catalyst relative to oil weight, at temperature 60℃ and mixing rate 1000 rpm after 120 min of transesterification reaction achieved a yield of methyl esters 98.76%. With three reuses of hydrated lime, without intermediate washing and regeneration procedures, the yield of rapeseed oil methyl ester remained above 90%.

The world is currently at the beginning of the fourth industrial revolution – Industry 4.0, whose ultimate goal is to make everything intelligent, both production processes in the industry and system maintenance. The environment around us has plenty of piping systems such as: water, gas, oil, sewage, etc., which need to be continuously maintained. In other words, they require periodic inspections to identify errors such as corrosion, cracks, deformations, or obstruction with obstacles. Service robots for inspection and maintenance are very convenient for the inspection of piping systems. In addition, they are a point of interest to many researchers in the field, so there are countless developed service robots that are currently in use. Service robots for inspection of piping system are used for inspection and provide visual information from inside the corresponding pipe. When the service robot moves through the pipe, it records the inside with a camera and provides us with visual information, i.e., provides a video of the inside of the pipe where we can locate the error. We can use the video later to establish the condition of the recorded piping system and make the right decision what to do. The paper presents the trend of application of service robots for inspection. A number of constructions of these service robots that are already in implementation are shown. Service robots effectively reduce all problems related to the maintenance, cleaning and inspection of piping systems. The growing trend of service robots application is due to the implementation of basic technologies of Industry 4.0 because its aim is to receive the information about the operation of a system all along. Various robotic systems have been developed for inspection and examination of piping systems and plants that are dangerous to workers' health. Service robots are controlled by camera, sensor or simple tools. Most service robots for inspection are intended for tanks, piping systems for all materials for inspection of ventilation openings and pipes of air systems, sewer systems, nuclear plants, or work in aggressive environments. It is expected that the development and application of service robots for inspection will continue to grow in the nearest future. Service robots effectively reduce all problems related to the maintenance, cleaning and inspection of piping systems.

The reduction in the fresh water supply and increase in the domestic effluents with increase in population and urbanization in the Pakistan force the farmers to use untreated sewage water for the irrigation purposes. Besides high nutrient content Sewage water also have source of metal contamination in the food chain. The present field study was conducted to compare the nickel (Ni), copper (Cu) and lead (Pb) contamination in vegetables grown on soils irrigated with sewage water and canal water in Sargodha, Punjab, Pakistan. The Ni, Cu and Pb contamination was assessed using soil quality indices i.e., contamination factor (CF), metal translocation factor (MTF), pollution load index (PLI), geo-accumulation index (Igeo) and ecological risk index (ERI) were calculated in the collected samples. The physico-chemical properties of soil and water samples were determined. Based on the results, it was revealed that sewage irrigated areas were at higher risks of metals contamination compared to canal irrigated areas. From the studied metals, Pb showed highest contamination potential based on the soil quality indices. In sewage irrigated sites, metal concentrations were found higher in edible parts of the vegetables confirming that sewage water contains and supply more metals than canal irrigated water and pose more health and ecological risks.

Monitoring of the lineages SARS-CoV-2 is equally important in a fight against COVID-19 epidemics, as is regular RT - PCR testing. Ion AmpliSeq Library kit plus is a robust and validated protocol for library preparation, but certain optimizations for better sequencing results were required. Clinical SARS-CoV-2 samples were transported in three different viral transport mediums (VTM), on arrival at the testing lab, samples were stored on -20OC. Viral RNA isolation was done on an automatic extractor using a magnetic beads-based protocol. Screening for positive SARS-CoV-2 samples was performed on RT–PCR with IVD certified detection kit. This study aims to present results as follows: impact of first PCR cycle variation on library quantity, comparison of VTMs with a quantified library, maximum storage time of virus and correlation between used cDNA synthesis kit with generated target base coverage. Our results confirmed the adequacy of the three tested VTMs for SARS-CoV-2 whole-genome sequencing. Tested cDNA synthesis kits are valid for NGS library preparation and all kits give good quality cDNA uniformed in viral sequence coverage. Results of this report are useful for applicative scientists who work on SARS-CoV-2 whole-genome sequencing to compare and apply good laboratory practice for optimal preparation of the NGS library.

Starting from the emergence of 1st Generation network (1G), wireless mobile communications have been undergoing an evolution - from 2nd Generation (2G), 3rd Generation (3G), 4th Generation (4G) networks to 5th Generation network (5G) at present. The fifth era is only a continuation of the ongoing evolution as it is still in the research phase and is also the basis for further development of industries and the society in general. The paper presents and compares the fourth and fifth generation of wireless mobile communications, focusing on the differences and progress in terms of data transmission rate, capacity, architecture, technology and applied multiple-technique approaches and services provided.

Abstract Introduction Professional drivers’ knowledge about driving-impairing medications is not satisfactory. The aim of this study was to develop and test the reliability and validity of the questionnaires designed to measure the knowledge and attitude of professional drivers about the influence of various medications on driving ability. Methods The questionnaires for assessing professional driver’s knowledge (performance-based) and attitudes about influence of various medications on driving abilities were developed by creating the item pool, testing reliability and validity, and factor analysis. The study was conducted as a multicenter, cross-sectional study in Serbia and Bosnia and Herzegovina. The study population consisted of professional drivers, who filled out both questionnaires in three time intervals. Results Both questionnaires showed great internal consistency and temporal stability. Cronbach’s Alpha for the first questionnaire was 0.984 and for the second it was 0.944. The Kaiser–Meyer–Olkin test for the first questionnaire confirmed sampling adequacy with its value of 0.964 and for the second questionnaire it was 0.933. Exploratory factor analysis of the questionnaire showed that three factors were revealed after rotation for the first questionnaire and they explained 78.0% of variance. Both questionnaires showed high degree of correlation between scores after the first and repeated administration, Spearman’s rho coefficient of correlation for was 0.962 and 0.980. Conclusion Based on the results of this study, we believe that both questionnaires are useful tools for testing professional drivers’ knowledge and attitudes about the influence of medications on driving ability.

Globalization has led to the spread of English, which has become the world’s international language. As a result of its importance in social, economic, and political spheres, many countries have introduced English language learning in their school curricula. However, despite its importance many learners still seem to struggle with English second language (L2) motivation, which is an important element of L2 learning success. The aim of this study was to analyse the relationship between several phases of learners’ motivation, including learners’ attributions of past English L2 learning success, current motivational characteristics, and motivation to learn English in the future. Using the L2 Motivational Self System (L2MSS) as a framework, it was found that past learning experiences did not have a significant effect on current learner motivational dispositions. However, elements of the L2MSS had an effect on learners’ intentions to exert effort into future English language learning. In other words, Croatian university students’ L2 motivation was based on their visions for the future rather than on past learning experiences. Moreover, it appears that the ideal L2 self plays a much stronger role in learners’ motivation compared to the ought-to L2 self, and this motivation is tied to utilitarian motives for learning English. In addition, the results showed that other motivational components, such as interest, as well as L2 anxiety, are important elements of learners’ motivation, suggesting cognitive as well as affective aspects in this motivational profile. The complexity of motivation as shown in this study suggests the need for further investigations in various contexts.

The genus Artemisia (fam. Asteraceae) is one of the largest and widely distributed with around 500 species, majority used as aromatic and medicinal plants. Artemisia annua L. is widely used as a dietary spice, herbal tea, as a supplement, and in a non-pharmaceutical form for treatment of malaria and fever. It is orally consumed as capsules, extracts and tinctures and topically applied as an essential oil diluted in lotions and ointments. Artemisinin is the main constituent of Artemisia annua L. extracts. Since the discovery that the artemisinin is efficient in malaria treatment, there is also a growth in consumption of A. annua extracts for antitumour and even recently for antiviral treatments against SARS-CoV-2 infections. This study aimed to investigate genotoxic effect in peripheral blood culture and cytotoxic effects in cancer and normal cell lines, of commercially available A. annua L. tincture in series of dilutions. Both comet and neutral red uptake assays revealed dose-dependent genotoxicity and cytotoxicity of A. annua tincture dilutions. Comet assay revealed significantly increased DNA damage in peripheral blood cells while neutral-red assays showed increase in cytotoxicity (p<0.001) in both normal and cancer cell cultures treated with the lowest extract dilution compared to the highest one applied. Obtained results indicate caution needed in A. annua L. tincture use, especially when poorly diluted.

CAT gene encodes catalase, a key antioxidant enzyme in the body against oxidative stress. This enzyme plays an important role in the molecular mechanisms of inflammation, apoptosis, mutagenesis and tumorigenesis. Anti-oxidant L-carnitine is used in food supplementation, medical co-treatment and bodyweight regulation. We aimed to investigate molecular basis of L-carnitine commercial preparations supplementation in reducing oxidative stress with customized CAT gene assay in vitro. Human lymphocytes cell culture was established using standard procedure and treated with range of concentrations of L-carnitine in two preparations. We tested two preparations: 500 mg tablets of L-carnitine and liquid L-carnitine with vitamin B6. L-carnitine significantly reduced the expression of CAT gene in cultured lymphocytes at concentrations of 50 μmol/l and 250 μmol/l compared to negative control, (p = 0,001; p = 0,001; respectively). The L-carnitine liquid supplement with vitamin B6 also reduced the transcription of CAT gene at concentrations of 50 μmol/l and 250 μmol/l as compared to the negative control (p = 0,018; p = 0,006; respectively). Selected L-carnitine preparations modulated the transcriptional activity of the antioxidant enzyme gene in human lymphocyte culture, indicating its possible effects in inhibition of pro-inflammatory processes that involve catalase activity.

Abstract Rare diseases (RD) are serious chronic diseases affecting small number of people compared to the general population. There are between 6000 and 8000 RDs, which affect about 400 million people worldwide. Drugs used for causal treatment of RDs are called orphan drugs. RDs bear great clinical and economic burden for patients, their families, healthcare systems and society overall. There are at least two reasons for the high cost of treatment of RDs. First, there is no causal therapy for majority of RDs, so exacerbations, complications, and hospitalizations in those patients are common. The second reason is high price of available orphan drugs, which are not cost-effective when traditional pharmacoeconomic evaluation is employed. The pharmacoeconomic aspect of the treatment of RDs is especially important in the field of neurology, since at least one fifth of all RDs is composed of neurological conditions. The aim of this paper was to provide a concise overview of the pathophysiological, epidemiological and clinical characteristics of some of the most important and common rare neurological diseases, with special reference to their impact on society and economy.

Purpose: The aim of the study was to determine the differences between football players and basketball players in the mean absolute values ​​of maximum torque flexors and extensors, ratio of maximum hamstring torque to maximum quadriceps torque dominant (DOM) non-dominant (ND) leg and differences in bilateral imbalance of flexor muscles and knee extensors. Material and methods: The research included a sample of 39 professional athletes. The first subsample included 19 professional basketball players while the second subsample included 20 professional soccer players. Results: Based on the results of the torques of the extensors in the knee joint of the DOM and ND legs, it was established that there is no statistically significant difference between basketball players and football players. However, a statistically significant difference was found in the torque flexors of the knee joint DOM (p≤0.01) and ND (p≤0.00) of the leg between basketball players and football players. On the other hand, the results of the research indicate that the difference between basketball players and football players in the ratio of Hamstrings peak torque to Quadriceps peak torque was recorded only in the ND leg (p≤0.02), while the difference in the DOM leg is not statistically significant. The results of our study indicate that basketball players have a higher percentage of imbalances compared to football players, especially in m. hamstrings. Conclusion: This study provides normative data on populations specific to soccer and basketball, but does not provide evidence of the ability of the isokinetic assessment of lower extremity muscle strength to predict injuries to football players and basketball players.

Purpose: to determine the most significant kinematic indicators in the sports selection of beginner shot putters. Material and Methods: This study was carried out on a sample of 9 students at the fourth stage of the competition in Division 1, which took place in the 2017/2018 academic year at the Faculty of Physical Education of Maysan University. The following kinematic (biomechanical) parameters were analyzed: the angle of release of the nucleus, the velocity of release, the height of the point of ejection of the nucleus and the speed of swing. The correlation coefficients were determined between the kinematic indicators and the result in the shot put, as well as the regression equation for the dependence of the result in the shot put on the knematic indicators. The data obtained in the study were presented in the form of the arithmetic mean, standard deviation, median, skewness coefficient, Pearson's correlation coefficient, analysis of variance and linear regression, which included the contribution coefficients of each analyzed indicator, standard error, reliability of the regression equation as a whole, and reliability of the coefficients contribution to the shot put result of each kinematic exponent. Results. It has been shown that the swing speed has the greatest influence on the result in the shot put among beginner athletes. The swing speed, shot angle, shot speed and shot height have significant relationships with the shot put result. The multiple regression equation for the dependence of the shot put result on the swing speed, shot angle, shot height and shot point turned out to be reliable in general. However, only the swing speed has a reliable coefficient of the regression equation. The shot angle tends to be the determining factor in the shot put result. The release rate and the height of the release point have significant correlations with the shot put result, although in the regression equation they have unreliable indicators of influence on the shot put result. Conclusions. When teaching beginner shot putters, the greatest attention should be paid to the pushing swing technique, namely the swing speed. The second most important indicator is the angle of the shot put, it is recommended to use the basic prediction equation, which determines the expected results in the selection of young athletes in shot put, with high reliability of the results obtained. These characteristics are recommended to be used for evaluating young athletes, as well as in the process of training and preparing athletes for competitions.