ABSTRACT

Background: The aim of this study was to compare the yields of acetylsalicylic acid synthesis based on the traditional approach and green chemistry approach. Green chemistry is one of the most important approaches in pharmaceutical sustainability. The reagents used for the acetylsalicylic acid synthesis can be environmentally harmful, so there is a need for a better and safer alternative. Methods: We conducted five syntheses based on the traditional approach and five syntheses based on the green chemistry approach. The synthesis process was identical except for one part – traditional synthesis used sulfuric acid and green chemistry synthesis used phosphoric acid. After synthesis, theoretical and experimental yields were calculated. The obtained products were tested using high performance liquid chromatography.Main findings: The theoretical yield of acetylsalicylic acid in both reactions was determined as 3.896 g. Traditional synthesis gave us a mean yield of 3.113 g (79.8%). Green chemistry synthesis gave us a mean yield of 3.104 g (79.4%). The quality of both products was confirmed by the above-mentioned chromatography method. There was no statistically significant difference between the means of the product masses (p=0.8220).Principal conclusions: Phosphoric acid can be used as a safe alternative to acetylsalicylic acid synthesis instead of sulfuric acid. As phosphoric acid is much safer than sulfuric acid in terms of environmental impact, these findings can be used in the pharmaceutical industry setting to obtain a better, cleaner and safer alternative to acetylsalicylic acid synthesis.Key words: acetylsalicylic acid, synthesis, green chemistry, medicinal chemistryArticle processing history:Received July 19, 2023 Revised November 7, 2023 Accepted November 21 , 2023 ORCID IDs of the authors: L.G. 0009-0005-9137-6218M.K. 0000-0003-3904-4994Corresponding author:Assistant Professor Martin Kondža, PhD; Faculty of Pharmacy, University of Mostar, Matice hrvatske bb, 88000Mostar, Bosnia and HerzegovinaE‒mail: martin.kondza@farf.sum.baCite this article as: Golemac L, KondžaM. Synthesis of acetylsalicylic acid –an environmentally friendly approach. Annals of Biomedical and Clinical Research. 2023;2:100 -108 . https://doi.org/Copyright © School of Medicine, University of Mostar 2023

Background: The aim of this study was to determine type and frequency of complications during multiple pregnancies, mode of delivery and perinatal outcome in three groups of pregnant women, categorized by gestational age of pregnancy.Methods: In cross-sectional epidemiological study data were collected and processed from the medical database of University Clinical Hospital in Mostar in the period between 2015. and 2019. A total of 149 pregnant women and their neonates were included and divided into three groups according to gestational age: moderately preterm birth (from 22 to 33 + 6 weeks - MPTB), late preterm birth (from 34 to 36 + 6 weeks - LPTB ), term birth (from 37 weeks). SPSS for Windows software (version 23.0, SPSS Inc, Chicago, Illinois, USA) was used for statistical analysis.Main findings: Of the total 149 pregnancies, 64.86% were completed by C-section and it was the dominant mode of delivery in all gestational groups. Hypertension, PPROM and gestational diabetes pregnancy were the most common complications in the group of term birth, PPROM and preeclampsia in the LPTB group, multiple maternal complications in the group MPTB (p <0.001). In both twins, respiratory distress syndrome (RDS) was the most common complication in MPTB group, and neonatal jaundice and perinatal infection and sepsis in the LPTB and term birth group.Principal conclusions: Caesarian section was dominant mode of delivery in all groups. RDS and multiple complications were significantly more common in MPTP group, and neonatal jaundice, perinatal infections and sepsis in LPTP and term birth group.Key words: multiple pregnancy, PPROM, preeclampsia, hypertension, perinatal complications, caesarian section.

Background: The aim of this paper was to investigate the dynamics of inflammatory parameters in patients after infrarenal abdominal aneurysm surgery.Methods: The retrospective study included patients with operated infrarenalabdominal aneurysm, divided into two groups. First group were patients that underwent endovascular aneurysm repair procedure (EVAR), and second group were patients that had open surgery. General data and inflammatory parameters (CRP, leukocytes, platelets, and fibrinogen) had been taken during hospitalization, compared mutually and analyzed.Main findings:All inflammatory parameters, observed preoperatively, were close to referent range. Serum CRP values in EVAR patients raised after operation, but returned to the reference range more quickly as well as leukocyte count. Around the middle of their own hospital stay and at the day of discharge, the group operated on by the open method had statistically significant higher platelet values than the EVAR group. During the preoperative period and in the middle of hospitalization, the group operated on by the open method shows higher fibrinogen values, while the opposite is at the day of discharge, where the EVAR group shows statistically significantly higher fibrinogen values.Principal conclusions: Observed serum inflammatory parameters (CRP, leukocytes, fibrinogen, and platelets) in different phases of hospitalization were lower; closer to the reference value in patients with infrarenal abdominal aneurysm that underwent EVAR, compared to patients operated by the open surgery method. Also, the observed parameters, except fibrinogen, returned to the reference value faster in patients who underwent EVAR.Key words: abdominal aortic aneurysm, endovascular aneurysm repair, inflammatory parameters

U članku se raspravlja o upotrebi govornih činova pozdravljanja i zahvaljivanja kod učenika srednjoškolskog uzrasta i važnosti ovih komunikacijskih jedinica u svakodnevnom ophođenju u školskom i vanškolskom okruženju. Poznato je da ljudi govornim činovima obavljaju specifične društvene aktivnosti. Pozdravljanje i zahvaljivanje predstavljaju jednostavne govorne činove iz područja fatičke komunikacije koji se odlikuju visokim stupnjem konvencionaliziranosti, ritualnosti i stereotipnosti i pokazatelji su kulturno preporučenog i prihvatljivog ponašanja u društvu. Mnogi govorni činovi u određenoj zajednici obuhvaćaju ustaljene, često formulaične obrasce koji su kolektivno usvojeni odnjezinih pripadnika. Tu svakako spadaju i pozdravi i zahvale kao visoko tipizirani fatički iskazi koji se ubrajaju u skupinu predstrukturiranih jezičkih obrazaca u okviru institucionaliziranih govornih činova. Govorni činovi pozdravljanja i zahvaljivanja imaju značajne pedagoške vrijednosti jer igraju ključnu ulogu u razvoju komunikacijskih i socijalnih vještina kod djece i mladih. Cilj je rada pokazati načine jezičkoga ostvarivanja navedenih govornih činova kod srednjoškolaca te utvrditi i analizirati njihove stavove o važnosti takve vrste jezičkog ponašanja i djelovanja s aspekta iskazivanja ljubaznosti i zauzimanja odgovarajućeg stava prema drugim ljudima i u odgojno-obrazovnoj praksi i u svakodnevnoj komunikaciji izvan školskog okruženja. Posebno je značajno ukazati i na obrazovnu potrebu za poučavanjem o važnosti pozdravljanja i zahvaljivanja u nastavnoj praksi u kontekstu kulture izražavanja i jezičkog bontona. Istraživanje je provedeno anketiranjem srednjoškolaca triju sarajevskih gimnazija, s pretpostavkom da općenito postoje razlike u izvođenju ispitivanih govornih činova u školskom okruženju i izvan njega. Također se očekuje da su navedene nepodudarnosti uvjetovane društvenim statusom komunikatora, stupnjem bliskosti odnosno distanciranosti, dobnim ili spolnim razlikama te porodičnim i drugim vrijednostima i običajima.

The article is devoted to the actual issues of individual labor dispute resolution. In particular, the author draws attention to the fact that in connection with the armed aggression of the Russian Federation, quite a large number of workers found themselves in a situation of forced migration, and some enterprises remained in temporarily occupied territories or faced the problem of the impossibility of relocation, which causes problems in the normal development of labor relations between the employee and the employer. An analysis of the current labor legislation and the existing approaches in the legal literature regarding the procedure for resolving individual labor disputes was carried out. The author highlighted the following reasons for labor disputes, including reduction of the main staff, changes in existing working conditions, dissatisfaction of the employee with the existing level of remuneration and others, in particular, and the martial law, which caused a number of reasons that impact on the occurrence of labor disputes - suspension of labor relations, the relocation of enterprises from the occupied territories and the impossibility of ensuring the safety of workers, downtime. These obstacles cause the problems in the relationship between the employer and the employee, resulting in the employees being forced to defend their rights. Conclusions were made by the author that, to date, the labor legislation of Ukraine ensures the implementation of the right to protect the rights of employees in individual labor relations in the pre­trial (commissions on labor disputes and through mediation) and the judicial procedure. It should be noted that an attempt was made by the legislator to regulate labor relations during the legal regime of martial law by adopting the Law of Ukraine «On the Organization of Labor Relations in the Conditions of Martial Law», but it did not define the issues related to the resolution of individual labor disputes, as well as the practice of its application provisions to date is ambiguous, which is confirmed by a number of court cases.

<p><strong>Introduction.</strong> Denture-induced fibrous hyperplasia (DFH) is a reactive lesion that occurs as a result of chronic trauma caused by the denture on the oral mucosa.</p> <p><strong>Case report.</strong> A female patient, 56 years old, reported to the Specialist Center for Dentistry at the Faculty of Medicine in Foča for the fabrication of complete dentures. She wore previous complete dentures for about 25 years and complained about their age and discoloration. Clinically, both dentures were poorly retained and stabilized by short wings, with pronounced signs of wear. Denture hygiene was poor. Multiple folds of hyperplasic connective tissue were observed in oral cavity upper vestibule mucosa. The lesion folds were pink and firm, corresponding to denture edges. Based on the anamnesis and clinical examination, a temporary diagnosis of denture-induced hyperplasia was made. The patient was informed about clinical condition assessment as well as possible therapeutic procedures and gave written consent to accept the offered therapeutic procedures. She was advised not to wear dentures for four weeks and to maintain proper oral hygiene. After that, the hyperplasia was surgically removed under local anesthesia. A tissue sample was sent for pathohistological analysis. A new pair of total dentures was made. Removed tissue pathohistological examination confirmed the diagnosis: &ldquo;Epulis fissuratum&rdquo;.</p> <p><strong>Conclusion.</strong> Education and regular check-ups are essential for the prevention of DFH. Patients should be given detailed oral and written instructions on oral and denture hygiene maintenance, with special reference to possible changes in oral tissues and the importance of regular follow-ups.</p>

<p><strong>Introduction. </strong>Long-term care is series of health and social care services provided at home or in an institutional environment to people with reduced functional capacity. Even though it is known that the quality of life (QoL) of elderly is on a lower level when compared to younger people, there is a small amount of information about QoL of long-term health care patients. The aim of our study was to determine the difference in QoL, depression, anxiety and stress of longterm health care users placed in and outside the institution.</p> <p><strong>Methods.</strong> This cross-sectional study included 100 long-term healthcare users, divided in two groups. First group consisted of 50 respondents from primary level of health care and second group consisted of 50 residents of nursing home. The research was conducted in the period from June to July 2022. A general socio-demographic questionnaire, SF-36 health questionnaire for estimating the QoL and the scale of depression, anxiety and stress (DASS-21) were used as instruments in this study.</p> <p><strong>Results. </strong>Respondents who were institutionalized had significantly lower values of domains and summary scores of QoL compared to respondents whose health care took place outside of institutions, where the difference was observed in physical health summary score (37.67&plusmn;6.20% vs. 50.39&plusmn;10.74%, p&lt;0.001), and the mental health summary score (44.87&plusmn;11.82% vs. 51.83&plusmn;16.01%, p =0.015). Respondents who received long-term health care institutionally had significantly (p=0.011) more mild anxiety (12%) and moderate anxiety (34%) compared to respondents who received long-term health care outside the institution (4% vs. 14%).</p> <p><strong>Conclusion. </strong>Our results showed that residents of nursing home had significantly worse physical and mental functioning, as well as more frequent occurrence of anxiety symptoms compared to users of longterm health care placed outside institutions, in home environment.</p>

Angiogenesis is the process of forming new blood capillaries from pre-existing vessels. Even though it is essential during normal development, it plays a major role in cancer progression. Neratinib is a pan-human epidermal growth factor receptor (HER) inhibitor that has recently been approved for the treatment of HER2-positive breast cancer. However, its effects on angiogenesis and embryogenesis remain unknown. This study examined the antiangiogenic effects of neratinib using the chorioallantoic membrane (CAM) of chicken embryos. We also evaluated neratinib’s toxicity during the early stages of normal development using the chicken embryos, primary embryonic fibroblasts (EFBs), and human umbilical vein endothelial cells (HUVEC). Our findings revealed that neratinib significantly inhibited the CAM angiogenesis compared to controls by reducing vessel percentage area and the average vessel length. Furthermore, neratinib downregulated vascular endothelial growth factor (VEGF), a key mediator of angiogenesis. At lower concentrations, neratinib was well-tolerated during early stages of normal development. Additionally, EFBs treated with neratinib showed no morphological or viability changes when compared to controls. However, at the highest concentration tested, neratinib treatment reduced HUVEC cell viability. This effect may be associated with the dysregulation of key apoptotic genes, including caspase-3, caspase-8, caspase-9, and the B-cell lymphoma 2 (Bcl2) gene. Our findings indicate a novel potential application of neratinib as an antiangiogenic agent, exhibiting tolerable toxicity in the early stages of embryogenesis.

Bosnia and Herzegovina (BiH), a country rich in history and culture, has for centuries been at the crossroads between the West and the East, which has resulted in quite a mixture of cross-cultural elements that pose many obstacles for translators. As the issues of tourism-language translation have been relatively underexplored in BiH, this article attempts to investigate the extent to which the translators of tourism-related content available online succeeded in making the local color of architecture-related terms accessible to foreign visitors. The article also examines whether the issue of accessibility of local color was addressed adequately by local translators. Many formal and informal online sources were used for the selection of prominent cultural terms and their English translations, which were analyzed in terms of lexis and semantics. We identified many examples where the cultural content was made accessible to the target audience, as well as examples where the transfer of the cultural content was done less successfully. In cases where translators experienced difficulty while transferring cultural terms, we attempted to draw prospective translators’ attention to potential pitfalls in the process of cultural mediation in translation from BiH’s official languages into English. Keywords: Bosnia and Herzegovina, tourism online, mediation, (in)accessibility, local color, translation, architecture

<p>Diabetic retinopathy (DR) is a chronic microvascular complication of diabetes. Due to the dramatic increase in the number of diabetes cases, the prevalence of DR has also risen, making it the leading cause of blindness among the working-age population worldwide, despite the availability of screenings and modern therapeutic options. Risk factors for the development and progression of DR (duration of diabetes, hypertension, hyperglycemia, dyslipidemia, and genetic factors) have been investigated in numerous epidemiological studies and clinical researches, but the research results were not consistent. In recent years, there has been considerable interest in the study of dyslipidemia in diabetes as one of the factors that could influence the onset and progression of DR, as well as apolipoproteins as potentially better biomarkers for DR. The results of our research also point in that direction. Identifying the risk factors for DR is crucial for enabling adequate prevention and raising awareness among individuals with diabetes about the importance of taking appropriate measures to prevent this microvascular complication.</p>

Rad na evidenciji i obradi stećaka Bosne i Hercegovine, koji se kontinuirano provodi putem usvajanja Odluka Komisije za očuvanje nacionalnih spomenika o proglašenju određenih lokaliteta nacionalnim spomenicima Bosne i Hercegovine, redovno rezultira pronalaženjem većeg broja stećaka i ukrasa zastupljenih na njima, kao i stećaka s natpisima pisanim srednjovjekovnom bosanskom epigrafskom ćirilicom. Nekropolu sa stećcima na lokalitetu Pitomi brijeg, na Morinama, u Nevesinju, pronašao je Gordan Čahtarević i evidentirao postojanje natpisa na pet stećaka, koji se u ovom radu prvi put objavljuju. Godine 2018. u mjesecu septembru i decembru izvršili smo opservaciju lokaliteta te utvrdili postojanje još dva natpisa. U ovom radu predstavljeno je dosadašnje čitanje Semoradovih natpisa s Boljuna te priređeno čitanje natpisa s lokaliteta Pitomi brijeg na Morinama u Nevesinju i lokaliteta Bojičića do na planini Crvanj u općini Kalinovik.

Vines in Bosnia and Herzegovina are traditionally grown in the Herzegovina region, where, in addition to many international varieties, indigenous and domesticated varieties are also successfully grown. The most important varieties are Žilavka and Blatina, while others are found in a much smaller area. One of these is the Radovača variety, which is preserved in only one micro-locality in Herzegovina region. The paper presents the results of a two-year analysis (2017 - 2018) of the qualitative characteristics of grapes and wines of the Radovača variety. The average cluster weight ranged from 195,50 to 308,97g, and the weight of 10 berries ranged from 23,67g to 29,79g. A favorable proportion of the weight of berries in the cluster weight (96,63 - 97,86 g/100 gcluster) compared to the proportion of petioles (2,14 - 3,37 g/100 g cluster) was observed. The year had a significant impact on the observed characteristics, considering that the analyzed parameters had a slightly higher value during 2017. Oscillations in the value of TSS consequently caused oscillations in the alcohol content of the wine (13,88 - 8,19 %v/v). The values ​​of TTA in grape juice and TA of wine in the years of research were quite uniform, which may also indicate the genetic specificity of this variety in this respect. The content of phenolic compounds in grapes was also significant, especially in seeds (428,74 - 977,27 mg/kg). Regardless of the fluctuations in the years of research, the Radovača variety has a certain oenological potential, which is especially important considering that it is an indigenous grape variety.